var textForPages = ["Trends in Natural Products 2024                                     PSE Young Scientists' Meeting                             Phytochemical Society of Europe (PSE)                                           21st - 24th May 2024, Brno, Czech Republic                   BIOACTIVE NATURAL PRODUCTS IN BIOLOGY,                                 PHARMACY AND COSMETICS                                              Book of Abstracts","","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                                Phytochemical Society of Europe                                  Trends in Natural Products 2024                                       Young Scientists' Meeting                                         th                      21  - 24  May 2024, Brno, Czech Republic                          st                      BIOACTIVE NATURAL PRODUCTS IN                               BIOLOGY, PHARMACY AND                                                COSMETICS                                                      Book of Abstracts                                                                                                                                 1","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                                                    Editors:                 Hana Brožová (Masaryk University, Brno, Czech Republic)                Martin Helcman (Masaryk University, Brno, Czech Republic)                Daniela Hlavatá (Masaryk University, Brno, Czech Republic)                Mária Gáborová (Masaryk University, Brno, Czech Republic)                Milan Malaník (Masaryk University, Brno, Czech Republic)                Lenka Molčanová (Masaryk University, Brno, Czech Republic)                Karel Šmejkal (Masaryk University, Brno, Czech Republic)                            CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives 4.0)                 Published by Masaryk University, Žerotínovo náměstí 617/9, 601 77 Brno, Czech Republic                 1st edition, Brno 2024                 © 2024 Masaryk University Press                 © 2024                 ISBN 978-80-280-0559-7                                                                                                          2","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                                    Welcome address                 Dear colleagues,                  On behalf of the Phytochemical Society Europe and Faculty of Pharmacy, Masaryk University,                I would like to express the welcomes and our pleasure to meet you at the conference Trends in                Natural  Products  2024,  Young  Scientists'  Meeting:  Bioactive  natural  products  in  biology,                pharmacy and cosmetics.                  In 2024, YSM is hosted in Hotel Passage, in Brno, Czech Republic. Brno, the second largest                city in the Czech Republic located in the South Moravian Region, is renowned for its historical                and cultural significance as well as its various economic contributions. Brno is strategically                situated  at  the  confluence  of  the  Svitava  and  Svratka  rivers.  At  the  time  of  the  provided                information, it boasted a population of approximately 390,000 residents, making it the second-                largest  city  in  the  Czech  Republic,  following  Prague.  The  greater  Brno  metropolitan  area                encompasses  nearly  700,000  people.  Brno  is  located  almost  in  the  geographical  center  of                Europe. Brno, the former capital of Moravia, holds a special place in the history and culture of                the South Moravian Region.                  The aim of the annually organized Young Scientists' Meeting is to give doctoral students and                postdoctoral fellows the opportunity to meet their experienced senior colleagues, and to present                their research on a broad scientific forum. Beside this, we would like to accent the personal                meetings, creation of (sometimes long lasting) contacts and possible networking of students                from many countries, we can therefore express the conference as worldwide.                 Judging the multicultural and international spirit of conference (almost 150 attendants from                more than 20 countries), we hope that this international environment will stimulate the creation                of contacts not only on scientific basis, but also socially important, will help to create friends,                and will help to strengthen the global community connected by an interest in medicinal plants,                natural compounds and their broad utilization in human life.                  I would like to thank the mayor of the city of Brno, JUDr. Markéta Vaňková, and the rector of                Masaryk  University  prof.  MUDr.  Martin  Bareš,  Ph.D.  The  conference  is  held  under  their                auspices.                 We wish you all a successful conference and a pleasant stay in Brno. Hopefully, it is not last                time that we can meet.                                                                       Karel Šmejkal                                                                      On behalf of Organisation Committee                                                                      May 2024, Brno                                                                                                                  3","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                                      Committees                Chair of the conference                Karel Šmejkal (Masaryk University, Brno, Czech Republic)                 Organisation committee                Hana Brožová (Masaryk University, Brno, Czech Republic)                Margita Dvorská (Masaryk University, Brno, Czech Republic)                Mária Gáborová (Masaryk University, Brno, Czech Republic)                Martin Helcman (Masaryk University, Brno, Czech Republic)                Daniela Hlavatá (Masaryk University, Brno, Czech Republic)                Dagmar Jankovská (Masaryk University, Brno, Czech Republic)                Milan Malaník (Masaryk University, Brno, Czech Republic)                Lenka Molčanová (Masaryk University, Brno, Czech Republic)                Erika Moudrá (Masaryk University, Brno, Czech Republic)                 Scientific committee                Randolph Arroo (De Montford University, Leicester, UK)                Pinarosa Avato (University of Bari, Bari, Italy)                Petr Babula (Masaryk University, Brno, Czech Republic)                Søren Bak (University of Copenhagen, Denmark)                Franz Bucar (Graz University, Graz, Austria)                Lucie Cahlíková (Charles University, Hradec Králové, Czech Republic)                Paula Castilho (Madeira University, Madeira, Portugal)                Stefano Dall'Acqua (Padova University, Padua, Italy)                Thomas Efferth (Johannes Gutenberg-Universität Mainz, Mainz, Germany)                Francesco Epifano (University of Chieti-Pescara, Chieti, Italy)                Jaroslav Havlík (Czech University of Life Sciences, Prague, Czech Republic)                Pavel Klouček (Czech University of Life Sciences, Prague, Czech Republic)                Ilina Krasteva (University of Sofia, Sofia, Bulgaria)                Marina Kritsanida (Université Paris Cité, Paris, France)                Simon Vlad Luca (Technische Universität München, Munich, Germany)                Francisco A. Macías (University of Cadiz, Cadiz, Spain)                Milan Malaník (Masaryk University, Brno, Czech Republic)                Kenneth Ritchie (John Moores University Liverpool, Liverpool, UK)                Celine Riviere (Université de Lille, Lille, France)                Satyajit Sarker (John Moores University Liverpool, Liverpool, UK)                Krystyna Skalicka-Woźniak (Medical University of Lublin, Lublin, Poland)                Didem Şöhretoğlu (Hacetteppe University, Ankara, Turkey)                Miroslav Strnad (Palacký University, Olomouc, Czech Republic)                Karel Šmejkal (Masaryk University, Brno, Czech Republic)                Maria José Umbelino Ferreira (Universidade de Lisboa, Lisbon, Portugal)                Evelyn Wolfram (Zurich University of Applied Sciences, Wädenswil, Switzerland)                                                                                                                            4","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                       List of Plenary Lectures (Listed according to appearance in program)                    PL1     Francisco A. Macías                         In Nature, nothing is what it seems. Looking for multipurpose bioactive hybrids                 PL2     Maria José Umbelino Ferreira                         Alkaloid derivatives for targeting drug resistance in cancer                 PL3     Didem Şöhretoğlu                         Application of computational methods to phytochemical drug discovery                 PL4     Satya Sarker                         Natural skin-whitening agents                 PL5     Kenneth Ritchie                         Phytochemical inducers of the antioxidant response                 PL6     Petr Babula                         Plant secondary metabolites in wound healing                 PL7     Giovanni Appendino                         Phytocannabinoids: Reflexions from a two decade journey                 PL8     Lutfun Nahar                         The magical story of coffee and its benefits in skincare                 PL9     Attila Hunyadi                         Insect molting hormones: from ecological significance to drug discovery                 PL10  Meghan Lucas                         Getting published in 2024 and beyond: How to adapt to a rapidly changing                         publishing landscape                 PL11  Francesco Epifano                         Auraptene: a tale of a natural product                 PL12  Céline Rivière                         Natural products and synthetic derivatives as anti-coronavirus agents                 PL13  Pavel Mučaji                         Analysis of agrimony extracts in wound healing                 PL14  Miroslav Strnad                         Natural adenines exhibit an incredible range of biological activities                 PL15  Franz Bucar                         Antiadipogenic effects of coumarins from Citrus x limon                 PL16  Szabolcs Béni                         Enhancing oligosaccharide characterization: Leveraging the power of NMR                         spectroscopy                 PL17  Simon Vlad Luca                         Integration of liquid-liquid chromatography in natural product research                 PL18  Christian Zidorn                         Essentials in the acquisition, interpretation, and reporting of plant metabolite                         profiles                 PL19  Evelyn Wolfram                         Quality, safety and efficacy of natural health product – how HPTLC, bioassays, and                         KI can contribute to evidence                 PL20  Dra. Mariola Macías Lecture – Jesús G. Zorrilla                         Taking advantage of allelopathy: Discovery of natural products and design of new                         strigolactone analogues for weed management                 PL21  Randolph Arroo                         CYP1-catalysed bio-activation of the polymethoxy flavone chrysosplenetin                                                                                                             5","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 PL22  Stefano Dall'Acqua                         The challenges in the study of bioactive natural products using comprehensive                         analytical approaches                 PL23  Lucie Cahlíková                         Semisynthetic derivatives of galanthamine and their biological activity                 PL24  Adam Matkowski                         Corn-Cockle phytochemical cocktail – How to make good stuff from old weed?                                                                                                                                                                                        6","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL1                    In Nature, nothing is what it seems. Looking for multipurpose bioactive                                                         hybrids.                                        1,                   Francisco A. Macías *, Juan J. Merino Fernández, Miriam I. Martínez, Nuria Chinchilla,                                Francisco J. R. Mejías, Rosa M. Varela, José M. G. Molinillo                  1  Cadiz Allelopathy Group, Department of Organic Chemistry, School of Science, Institute of                 Biomolecules (INBIO), University of Cadiz, C/ República Saharaui nº 7, 11510-Puerto Real                                                       (Cádiz), Spain                                          *Corresponding author: famacias@uca.es                 Allelopathy emerged as an alternative to obtain new agrochemicals based on natural products,                which would be an alternative to classical herbicides due to the occurrence of weed resistance                phenomena  and reduce environmental impact.                           1                Our  previous  experience  in  this  area  has  allowed  us  to  develop  compounds  based  on                sesquiterpene lactones that exhibit high phytotoxic activity against different weeds as potential                pre-emergency  herbicides.  Bioactivity  and  other  properties  of  natural  compounds  obtained                directly from plants can be increased by preparing derivatives of natural sesquiterpene lactones.                More specifically, this is achieved through functionalization by introducing structural changes                in the α-methylene-γ-lactone ring with the simultaneous loss of C-13 exocyclic double bond.                 Recent years have witnessed significant advances in molecular hybridization to discover new                drugs . Inspired by this idea, our group has synthesized hybrids by combining active natural                      2                                                                              3                products to achieve a synergistic manifestation of the activities . Hybrids from sesquiterpene                lactones and benzohydroxamic acids retain the exocyclic double bond of lactone rings, which                taken individually exhibit a good profile of phytotoxic activity.                Those new hybrids were tested in phytotoxicity and cytotoxicity bioassays. Unexpectedly, a                decreased activity was observed in comparison to the constituent molecules. Therefore, in silico                assays  were  carried  out  by  us  using  credible  databases,  revealing  a  different  target:  the                inhibition of cellular hypoxia. Our synthesized hybrids seem to show a beneficial action to                cellular  functions,  preventing  oxygen  deficiency.  These  preliminary  results  display  a  new                exploration that was not anticipated by our hybrid molecules. Nevertheless, the initial insights                highlight the complexity of nature, where nothing is what it seems.                Acknowledgments:  This  work  was  supported  financially  by  the  Ministerio  de  Ciencias  e                Innovación (Project PID2020-115747RB-I00), Spain.                 References:                1.     T. S. Prather, J. M. Ditomasso, J. S. Holt. (2000). Publication 8012, Division of Agriculture and Natural                Resources, University of California. Doi:10.3733/UCANR.8012.                2.     Proschak,  E.  Stark,  H.,  Merk,  D.  (2019).  Journal  of  Medicinal  Chemistry,  62,  420–444.  Doi:                10.1021/acs.jmedchem.8b00760.                3.     Karagöz  AÇ,  Reiter  C,  Seo  E-J,  et  al.  (2018).  Bioorg.  Med.  Chem.  26:3610–3618.  Doi:                10.1016/j.bmc.2018.05.041.                                                                                                                7","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL2                          Alkaloid derivatives for targeting drug resistance in cancer                                                      Maria-José U. Ferreira                     Research Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, Universidade de                                             Lisboa, 1649-003 Lisbon, Portugal                                      *Corresponding author: mjuferreira@ff.ulisboa.pt                  Despite the number of strategies that have been developed in cancer treatment in recent decades,                it remains a leading cause of death globally, mainly due to drug resistance. Plants have been                playing a key role as source of bioactive compounds, namely anticancer agents. To obtain plant-                derived compounds with anticancer activity, we have focused on the phytochemical study of                plants  capable  of  producing  large  amounts  of  alkaloids,  allowing  further  molecular                derivatization.  Characterised  for  bearing  diverse  and  complex  scaffolds,  alkaloids  are                commonly characterized with strong biological properties.                Taking  advantage  of  their  functional  groups,  libraries  of  monoterpene  indole  and                Amaryllidaceae-type alkaloid derivatives have been generated.  The ability of natural alkaloids                and derivatives as ABC transporter inhibitors, namely as P-glycoprotein inhibitors, in different                resistant cancer cell lines has been  investigated through chemo-sensitization and functional                assays.  The antiproliferative activity of alkaloid derivatives has also been investigated in a                panel  of  human  cancer  cells.  Some  derivatives  showed  a  selective  antiproliferative  effect  on                resistant cells, having a collateral sensitivity effect.                 Acknowledgements:  Fundação  para  a  Ciência  e  a  Tecnologia  (FCT),  Portugal  (project                2022.05718.PTDC).                                                                                                                                              8","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL3                   Application of computational methods to phytochemical drug discovery                                                                             2                                                                1,                                              Didem Şöhretoğlu *, Suat Sari                 1  Department of Pharmacognosy, Hacettepe University, Faculty of Pharmacy, Ankara, Turkiye                   2  Department of Pharmaceutical Chemistry, Hacettepe University, Faculty of Pharmacy,                                                      Ankara, Turkiye                                      *Corresponding author: didems@hacettepe.edu.tr                   Natural  products  and  their  derivatives  have  historically  been  invaluable  source  for  drug                discovery. An increasing number of computational strategies are being applied to enhance and                speed up natural product-based drug design. Virtual screening has been widely used to identify                and optimize bioactive compounds including natural products since it reduces time and costs in                drug  discovery.  Numerous  molecular  modelling  tools  are  included  in  the  virtual  screening                process  of  natural  compounds. ADMET  and  druglikeness  filtering,  fingerprint  and  QSAR,                shape  and  pharmacophore  screening  are  among  the  techniques  where  ligand-based  data  is                widely used. On the other hand, structure-based methods such as molecular docking, molecular                dynamics simulations, and free binding energy calculation have gained momentum with the                increasing availability of macromolecular data and high-precision software. Combination of                ligand- and structure-based methods and using consensus approaches are currently becoming                all the more popular since these approaches have proven to outperform the applications where                a single method or approach is used . In the course of our research on Turkish medicinal plants                                                  1                to discover natural hits, we utilized different computational tools to find new  α-glucosidase                         2,3                inhibitors . We provide brief descriptions and applications of computational techniques and                approaches used in rational drug design. Discovery of natural products via these approaches is                presented to highlight the importance of computational methods in natural product drug design.                 References:                  1.     Sari,  S.;  Şöhretoğlu,  D.,  Chapter  12  -  Computational  approaches  to  phytochemical  drug                discovery. In Computational Phytochemistry (Second Edition), Sarker, S. D.; Nahar, L., Eds. Elsevier:                2024; pp 395-412.                2.     Şöhretoğlu,  D.;    Sari,  S.;    Barut,  B.;  Özel,  A.,  Discovery  of  potent  α-glucosidase  inhibitor                flavonols: Insights into mechanism of action through inhibition kinetics and docking simulations. Bioorg                Chem 2018, 79, 257-264.                3.     Şöhretoğlu, D.;  Sari, S.;  Šoral, M.;  Barut, B.;  Özel, A.; Liptaj, T., Potential of Potentilla                inclinata  and  its  polyphenolic  compounds  in  α-glucosidase  inhibition:  Kinetics  and  interaction                mechanism merged with docking simulations. Int J Biol Macromol 2018, 108, 81-87.                                                                                                                      9","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL4                                            Natural skin-whitening agents                                                                                                      3                                    1,                                                    2,                                                                        3                  Satyajit D. Sarker *, Lutfun Nahar *, Sarita Sangthong , Tinnakorn Theansungnoen  and                                                                       3                                                   Phanuphong Chaiwut                   1 Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool                                  John Moores University, Byrom Street, Liverpool L3 3AF, UK                   2 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The                            Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic                       3  School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand                               *Corresponding authors: S.Sarker@ljmu.ac.uk and nahar@ueb.cas.cz                 The skin is the largest and primary protective organ of the body, covering its entire external                surface and serving as a first-order physical barrier against the environment. The skin tone or                ‘complexion’ ranges from the palest hue to the darkest brown. The colour of the skin is not                someone’s choice but inherited by an individual. The amount of melanin, which is a tyrosine-                derived  complex  polymer,  defines  the  colour  of  the  skin.  In  certain  regions  of  the  world,                ‘beauty’ is often defined by fair skin colour, hence is the unsatiable quest for skin-whitening or                skin-lightening agents that may enhance ‘beauty’. This is not a new phenomenon; in fact, skin-                whitening attempts started in many civilizations about 200 BCA; for example, the use of honey                and olive oil as a method of whitening the skin in Egypt and Greece goes back centuries. From                the very beginning, natural products, (organic e.g., plant extracts, or inorganic e.g., mercury),                have played a major role as skin-whitening agents. Over the years, several plants, e.g., green                tea, pears, rose petal extract, olive oil, pomegranates, peony, Phyllanthus emblica and Rosa                roxburghii, have been identified to have skin-whitening properties and various skin-whitening                                            1                phytochemicals, e.g., arbutin  and kojic acid , have been reported in the published literature.                                                            2                Some of these natural products have been formulated into commercially available cosmetic                products. This talk will present an overview of skin-whitening natural products available to date                and comment on their safety aspects.                  Acknowledgements: We gratefully acknowledge Mae Fah Luang University Research Funding                (651B02002) and the Reinventing University fund from the Office of the Permanent Secretary                of the Ministry of Higher Education, Science, Research and Innovation, Thailand. Lutfun Nahar                gratefully acknowledges the financial support of the European Regional Development Fund -                Project  ENOCH  (No.  CZ.02.1.01/0.0/0.0/16_019/0000868)  and  the  Czech Agency  Grant  -                Project 23-05474S.                  References:                1.     Nahar, L., Al-Groshi, A., Kumar, A. and Sarker, S. D. Arbutin: Occurrence in plants and its potential as                an anticancer agent, Molecules 2022, 27, article number: 8786.                2.     Nurunnabi, T. R., Al-Majmaie, S., Nakouti I., Nahar, L., Rahman, S. M. M., Sohrab, M. H., Billah, M.                M., Ismail, F. M. D., Sharples, G. P. and Sarker, S. D. Antimicrobial activity of the ethyl acetate extract and its                major metabolite, kojic acid, from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia                apetala, a mangrove plant of the Sundarbans, Asian Pacific J. Trop. Med. 2018, 11, 350-354.                                                                                                              10","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL5                          Phytochemical inducers of the antioxidant response                                                                                1,3                                                                                             2                                                      1                                    1,                  Kenneth J. Ritchie *, Waed Alsheikh , Chuanchom Khuniad , Lutfun Nahar , Satyajit D.                                                                1                                                          Sarker                    1 Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular                     Sciences, Liverpool John Moores University, James Parsons Building, Byrom Street,                                             Liverpool L3 3AF, United Kingdom                  2 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany,                       The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic                   3 Department of Thai Traditional Medicine, Faculty of Health and Sports Science, Thaksin                                          University, Phatthalung, 93210, Thailand                                          *Corresponding author: k.j.ritchie@ljmu.ac.uk                 Cancer chemoprevention is the generic term used to describe the process of using a chemical                to prevent cancer. The prevention of cancer by such chemicals can generally be broken down                into four distinct areas: reducing inflammation, increasing apoptosis, increasing cell defence                genes and the prevention of metastasis. Currently, chemicals (both natural and synthetic) with                the ability to modulate any one of these cancer promoting processes are classed as cancer                chemopreventive agents. At the cellular level, much of the phytochemical modulation of cell                defence genes can be distilled down to the effects of one transcription factor, NF-E2 p45-related                factor-2 (Nrf2), which binds to the antioxidant response element (ARE) found in the regulatory                regions of over 200 genes involved in both cellular protection.                 In a quest to discover new phytochemicals with cancer chemopreventive capability, we have                utilised  a  cell-based  assay  to  identify  phytochemicals  with  the  ability  to  activate  the                transcription factor Nuclear factor-erythroid 2 p45-related factor 2 (Nrf2).  In this presentation,                our recent efforts to investigate the delicate balance that phytochemicals play in cancer will be                considered.                  Acknowledgement:  Lutfun  Nahar  gratefully  acknowledges  the  financial  support  of                the European     Regional     Development       Fund     -     Project    ENOCH        (No.                CZ.02.1.01/0.0/0.0/16_019/0000868) and the Czech Agency Grant - Project 23-05474S.                                                                                                                       11","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL6                                  Plant secondary metabolites in wound healing                                                       Petr Babula 1,2,3, *                  1  Department of Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic                   2  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Brno, Czech                                                         Republic                 3  Department of Sport Performance and Exercise Testing, Faculty of Sports Studies, Masaryk                                              University, Brno, Czech Republic                                        *Corresponding author: babula @med.muni.cz                   Wound healing is an extremely complicated process that reflects a number of factors, both                external  and  internal.  It  involves  a  cascade  of  events  that  includes  inflammation,                neovascularisation,  collagen  synthesis,  granulation  tissue  formation,  epithelialisation  and                                   1                wound remodelling . A number of different cell types participate in this process, starting with                fibroblasts, keratinocytes, adipocytes, endothelial cells, but also melanocytes or certain types                of leukocytes. At the same time, the wound healing environment is characterized by a very                dynamic redox environment, which somewhat extends our thinking about the importance of                reactive oxygen species. These have so far been discussed very often in the context of their                involvement in immune system responses, but their important role in signalling pathways is                         2                emerging .  This  presentation  discusses  the  possible  contribution  of  different  groups  of                secondary metabolites in the wound healing process with potentially practical applications.                   Acknowledgements:  Author  thanks  to  the  project  MUNI/A/1547/2023,  “Analysis  of                (dys)function: from molecules to living organisms”.                  References:                 1.     Peña,  O. A.,  Martin,  P.  Cellular  and  molecular  mechanisms of  skin  wound  healing.  Nature  reviews.                Molecular cell biology. 2024, Advance online publication. https://doi.org/10.1038/s41580-024-00715-1                2.     Krizanova, O., Penesova, A., Sokol, J., Hokynkova, A., Samadian, A., Babula, P. Signaling pathways in                cutaneous    wound     healing.   Frontiers    in    physiology.   2022,    13,    1030851.                https://doi.org/10.3389/fphys.2022.1030851                                                                                                                            12","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL7                           Phytocannabinoids: Reflexions from a two decade journey                                                    Giovanni Appendino*                         Dipartimento di Scienze del Farmaco, Largo Donegani 2, 28100 Novara, Italy                                    *Corresponding author: giovanni.appendino@uniupo.it                   Modern studies on Cannabis and phytocannabinoids can be traced to seminal investigations                carried out in 1940-1970 by Roger Adams in USA, František Šantavý in Czechoslovakia, and                Raphael Mechoulan in Israel, the latter also pioneering studies on their molecular targets and                                    1                clinical  applications .  Spurred  by  the  identification  of  cannabinoid  receptors  and  the                endocannabinoid  system,  biological  studies  on  phytocannabinoids  have  then  progressed                regularly, culminating in the clinical introduction of Cannabis-based mainstream medicines                                                              2                (dronabinol,  nabilone,  nabiximol,  cannabidiol) .  Conversely,  phytochemical  studies  did  not                progress  significantly  after  the  mid-eighties  of  the  past  century,  mainly  because  of  legal                restrictions on the study of Cannabis . Thanks to a EU-sponsored project and the collaboration                                                   3                with plant genetists and pharmacologists, I had the opportunity to start systematic studies on                what I considered uncharted areas of the phytocannabinoid chemical and biological space. Two                main research lines were pursued, namely, the study of native (acidic) phytocannabinoids, and                the one of minor phytocannabinoids. Acidic phytocannabinoids can be obtained in significant                amounts by isolation, while many minor phytocannabinoids are only trace constituents, and                their biological evaluation required the development of synthetic/semi-synthetic alternatives to                isolation.  Acidic  phytocannabinoids  are  prone  to  decarboxylation,  and  many  neutral                phytocannabinoids are then  easily oxidized under  aerobic  conditions. A critical part of our                phytocannabinoid project was therefore aimed at the study of this “programmed” chemical                diversity, and I will provide an outline of the most significant results obtained in exploiting the                exuberance of Cannabis as a phytocannabinoid factory for biomedical research.                   Acknowledgements: I am grateful to all colleagues and collaborators with whom I shared the                excitement of two decades of research on Cannabis and phytocannabinoids                 References:                  1.     Appendino G. The Early History of Cannabinoid Research. Rendiconti Lincei Scienze Fisiche e Naturali                2020, 31, 919-929.                2.     Di Marzo V. New Approaches and Challenges to Targeting the Endocannabinoid System. Nat. Rev. Drug                Discov. 2018, 17, 623-663.                3.     Hanuš, L. O.; Mayer, S. M.; Muñoz, E.; Taglialatela-Scafati, O.; Appendino, G. Phytocannabinoids: a                unified critical inventory. Nat. Prod. Rep. 2016, 33, 1357-1392.                                                                                                                 13","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL8                         The magical story of coffee and its benefits in skincare                                                                                                         2                                                    2                                1,                   Lutfun Nahar *, Sarita Sangthong , Tinnakorn Theansungnoen , Phanuphong Chaiwut ,                                                                                2                                                     Satyajit D Sarker                                                                     3                    1 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The                            Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic                       2  School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand                  3 Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool                                  John Moores University, Byrom Street, Liverpool L3 3AF, UK                                           *Corresponding author: nahar@ueb.cas.cz                 The world of skincare is continuously evolving, with new ingredients such as coffee being                utilized for their rejuvenating effects. This plenary talk will explore the ways in which coffee                benefits the skin. Coffee is rich in antioxidants, capable of repairing the skin, and shielding it                from environmental damage. Utilizing coffee grounds in face scrubs is highly effective as they                exfoliate dead skin cells, leaving the skin smoother and more radiant. Additionally, coffee beans                contain natural oils that moisturize the skin, making them valuable in various skincare products.                Recent  research  indicates  that  coffee  can  stimulate  the  production  of  collagen  and  elastin,                proteins crucial for maintaining skin elasticity and firmness. The primary active ingredient in                coffee is caffeine, which is known for its anti-skin-cancer properties and helps protect against                             1                sun  damage.   Coffee  also  contains  chlorogenic  acid  which  combats  free  radicals,  prevent                premature aging, and preserve youthful skin. Coffee grounds make excellent DIY face scrubs,                gently buffing away dead cells for a brighter complexion. These advantages have spurred the                development of new coffee-based skincare products designed to combat ageing.                  Acknowledgements: We gratefully acknowledge Mae Fah Luang University Research Funding                (651B02002) and the Reinventing University fund from the Office of the Permanent Secretary                of the Ministry of Higher Education, Science, Research and Innovation, Thailand. Lutfun Nahar                gratefully acknowledges the financial support of the European Regional Development Fund -                Project  ENOCH  (No.  CZ.02.1.01/0.0/0.0/16_019/0000868)  and  the  Czech Agency  Grant  -                Project 23-05474S.                  References:                1.     Elias, M. L., Israeli, A. F. and Madan, R. Caffeine in skincare: Its role in skin cancer, sun protection and                cosmetics, Ind. J. Dermatol. 2023, 68, 546-550.                                                                                                                         14","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL9                   Insect molting hormones: from ecological significance to drug discovery                                                       Attila Hunyadi *                                                                   1,2,                      1  Institute of Pharmacognosy, University of Szeged, Eötvös str. 6, 6720 Szeged, Hungary                  2  HUN-REN-SZTE Biologically Active Natural Products Research Group, Eötvös str. 6, H-                                                   6720 Szeged, Hungary                    Ecdysteroids represent the most widely distributed group of steroid hormones that play a highly                complex role in nature. In insects, 20-hydroxyecdysone is the so-called molting hormone. In                plants,  well  over  500  of  its  analogs  exist,  and  large  amounts  of  these  compounds  are                biosynthesized by many plant species as natural pesticides. In mammals, however, ecdysteroids                are neither hormones nor toxic compounds: Some are known to act as adaptogenic and anabolic                agents,  increase  resistance  to  stress,  and  provide  various  health  benefits.  Due  to  these,  a                worldwide  market  of  ecdysteroid-containing  food  supplements,  and  its  possible  abuse  in                competitive  sports  made  20-hydroxyecdysone  included  in  the  watchlist  of  the World Anti-                Doping Agency (WADA).                The wide chemical diversity of ecdysteroids makes these compounds a valuable pool for drug                discovery, and their semi-synthetic derivatives may interfere with cell death and survival in                either direction, i.e., using different chemical strategies, they can be engineered towards potent                antitumor  or  cytoprotective  agents.  Our  recent  studies  revealed  that  certain  ecdysteroid-                containing  nanoassemblies  sensitize  CNS-originated  tumor  cells  to  oxidative  stress  while                protecting the surrounding normal tissues.                The presentation provides an overview of the complex ecological role of ecdysteroids and on                our recent developments on their drug discovery perspectives.                   Acknowledgments: Financial support from the NRDIO, Hungary (K-146359 and TKP2021-                EGA-32) is acknowledged.                                                                                                                                  15","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL10                   Getting published in 2024 and beyond: How to adapt to a rapidly changing                                                 publishing landscape                                                                      1,                                                     Meghan Lucas *                       1  Chemistry \& Biodiversity, Wiley-VCH, Boschstr. 12, 69469 Weinheim, Germany                                         *Corresponding author: mlucas@wiley.com                    Let’s face it, the scientific publishing landscape has changed rapidly over the past years. From                print-only to online access, and now to a world where +90% of the papers are found either                through a search engine or social media. Interestingly, the process of bringing together your                research into a publishable manuscript has not changed that much, but our reading (or media                consumption) habits have changed drastically. I am going to show you various tips and tricks                that you can use to adapt your manuscripts to the “new publishing world” — It is now more                important than ever, not only to get published, but to get noticed.                                                                                                                                                         16","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL11                                        Auraptene: a tale of a natural product                          Francesco Epifano*, Serena Fiorito, Chiara Collevecchio, Salvatore Genovese                    Department of Pharmacy, University “Gabriele d’Annunzio” of Chieti-Pescara, Via dei                                         Vestini 31, 66100 Chieti Scalo (CH), Italy                                          *Corresponding author: fepifano@unich.it                    Auraptene (7-geranyloxycoumarin) 1 is the most representative compound of a rare class of                natural products, the oxyprenylated phenylpropanoids.                                                                   1                 Despite the fact that it has been isolated for the first time in 1930 from the peels and skin of                fruits  of  Citrus  aurantium  L.  (bitter  orange),  only  in  the  last  two  decades  this  secondary                metabolite has been subject of intensive research aimed at better characterizing new natural                sources,  its  phytochemical  and  nutraceutical  properties,  and  to  get  more  insights  into  its                biological activity and therapeutic potential. Like in a fairy tale with a happy ending lasting                more than 20 years, auraptene has been characterized among the most interesting and promising                active principle that can be obtained from a wide panel of plant sources.                At the very beginning of this “tale”, a single easy to handle and high yielding synthetic step                made auraptene 1 available in gram scale so that several in vitro pharmacological assays could                be performed. Thus, it has been found how compound 1 exerted numerous beneficial effects                mainly as an anti-inflammatory, anti-cancer, and neuroprotective agent. Moving to in vivo tests                using suitable and predictive animal models such properties have been confirmed. In parallel,                apart from the “classic” plant sources belonging to the Rutaceae family, investigations revealed                how auraptene represented an additional component of the phytochemical pool of medicinal,                healthy, and food plant species known to have beneficial properties for human health and for                the dietary feeding prevention of acute and chronic syndromes. The overall data accumulated                over  the  last  20  years  did  encourage  the  performance  of  a  clinical  trial  in  humans  using                phytopreparations derived from fruits of Citrus limon standardized in their composition for                auraptene and total flavonoids as a nutraceutical remedy for the prevention of Alzheimer’s                disease.                                                                                                                   17","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL12                     Natural products and synthetic derivatives as anti-coronavirus agents                                   1                                                                                           1,4                                                                                                      1,5                                                       2                                                                          1,2                  Gabriel Lefèvre , Imelda Raczkiewicz , Malak Al Ibrahim , Asma Hakem , Abda Ba ,                                      1                                                          3                                                                             1                    Lowiese Desmarets , Alexandra Hubert , Jennifer Samaillie , Lucie Borkova , Charline                                                                                             3                                                           1                             2                                                                                1                                                                                              1                    Camuzet , Zachee Louis Evariste Akissi , Anne-Sophie Paguet , Ramla Sahli , Sophie                                                                                                       2                           1                                                          2                                                                         4                                                                                      5                                          1                   Moureu , Sevser Sahpaz , Anne Goffard , Riadh Ksouri , Matar Seck , Jean Dubuisson ,                                                                3                                              2                           Sandrine Belouzard , Mourad Elhabiri , Karin Séron , Céline Rivière *                                                                                              1,                                                                              2                1  UMR 1158 BioEcoAgro, Univ. Lille, JUNIA, UPJV, Univ. Liège, INRAE, Univ. Artois, Univ.                  Littoral Cote D’Opale, ICV—Institut Charles Viollette, F59650 Villeneuve D’Ascq, France                    2  Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR9017 –                                      Center for Infection and Immunity of Lille (CIIL)                  3  CNRS - Université de Strasbourg - Université de Haute-Alsace, LIMA, CNRS UMR 7042,                  Equipe Chimie Bioorganique et Médicinale, ECPM, 25 Rue Becquerel, 67200 Strasbourg,                                                          France                    4  Laboratory of Aromatic and Medicinal Plants, Biotechnology Centre of Borj-Cedria                                            (CBBC), Hammam-Lif 2050, Tunisia                 5  Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et                  d’Odontologie de l’Université Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Sénégal                                     *Corresponding author: celine.riviere@univ-lille.fr                Coronaviruses (HCoVs) cause mild, moderate, or severe respiratory illnesses in humans. The                COVID-19 pandemic, caused by SARS-CoV-2, has had significant global health and economic                consequences.  Despite  significant  advances  in  understanding  the  pathology  and  clinical                management of coronaviruses, as well as creating effective vaccines, these viral diseases remain                a  public  health  concern  due  to  the  risk  of  recurrent  outbreaks  linked  to  the  emergence  of                variants, unsatisfactory vaccination rates in at-risk populations and a lack of specific antiviral                agents available against HCoVs. Our overall strategy is to identify pan-coronavirus antivirals,                leveraging naturally occurring structural diversity. This presentation will be an opportunity to                present  certain  collaborative  work  carried  out  which  has  highlighted  the  anti-coronavirus                potential of certain substances of natural origin and synthetic analogues.                 Acknowledgements: Authors  gratefully  thank  the  different  funding  organizations  for  phD                theses of Malak Al Ibrahim, Asma Hakem, Abda Ba and Imelda Raczkiewicz, as well as for                research programs ANR Flash FlavoCoV, CNRS prematuration and PHC Utique, in particular                Hauts-de-France region, University of Lille (I-Site), Campus France and CNRS Innovation.                References:                1.     Hakem, A, Desmarets, L, Sahli, R, Ben Malek, R, Camuzet, C, François, N, Lefèvre, G, Samaillie, J,                Moureu, S, Sahpaz, S, Belouzard, S, Ksouri, R, Séron K, Rivière C. Luteolin isolated from Juncus acutus L., a                potential   remedy     for    human      coronavirus   229E.     Molecules 2023, 28,   4263.                https://doi.org/10.3390/molecules28114263                2.     Al  Ibrahim,  M,  Akissi,  ZLE,  Desmarets,  L,  Lefèvre,  G,  Samaillie,  J,  Raczkiewicz,  I,  Sahpaz,  S,                Dubuisson, J, Belouzard, S, Rivière, C, Séron, K. Discovery of anti-coronavirus cinnamoyl triterpenoids isolated                from Hippophae rhamnoides during a screening of halophytes from the North Sea and Channel coasts in northern                France. Int. J. Mol Sci. 2023, 24, 16617. https://doi.org/10.3390/ijms242316617                                                                                                                18","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL13                                 Analysis of agrimony extracts in wound healing                                                                        1                                                         1                                 1,                                                                                     1                                                                                                1                    Pavel Mučaji *, Silvia Bittner Fialová , Milan Nagy , Elena Kurin , Peter Gál  , Ema                                                                             2                                                           1                                      1                                                                                                 3                         Kostovčíková , Kamila Dokupilová , Emil Švajdlenka , Martina Papadakos                    1 Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University,                                         Odbojárov 10, 832 32 Bratislava, Slovakia                   2  Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University,                                         Odbojárov 10, 832 32 Bratislava, Slovakia                  3  Department of Galenic Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov                                              10, 832 32 Bratislava, Slovakia                                      *Corresponding author: mucaji@fpharm.uniba.sk                  Agrimonia eupatoria L. is an herb of the Rosaceae family, widely used in traditional medicine                for its beneficial effects. The HMPC's conclusions on using agrimony medicines to relieve mild                diarrhea, mouth and throat inflammation, skin inflammation, and wounds are based on their                traditional use in these conditions. In previous studies, we established that Agrimonia eupatoria                L.  water  extract  (AE)  possesses  a  rich  polyphenolic  composition,  displaying  remarkable                antioxidant properties. Our research demonstrated its potential clinical utility in preventing and                managing cardiovascular complications linked to diabetes and enhancing skin wound healing                      1                in rats .                These findings led us to extend the evaluation to fibroblasts and keratinocytes, as well as open                and  sutured  wounds  in  rats,  and  to  formulate  a  topical  preparation  to  treat  minor  skin                inflammation and small, superficial wounds. The total phenolic liberating capability of prepared                gels was realized using a Franz diffusion cell system with different pH conditions. The in vitro                and in vivo experiments revealed that AE extract induced the myofibroblast-like phenotype and                enhanced extracellular matrix deposition in both experimental models. Furthermore, the wound                tensile  strength  of  skin  incisions  and  the  contraction  rates  of  open  excisions  significantly                increased in the AE-treated group.                 Conclusion: The present data show that AE water extract significantly improves the healing of                open and sutured skin wounds. Further testing in animal models that are physiologically and                evolutionarily closer to humans will be necessary.                 Acknowledgments: Authors thank Agency VEGA Grant No., 1/0226/22 and APVV-20-0017.                  References:                 1.     Kuczmannová A, Gál P, Varinská L, Treml J, Kováč I, Novotný M, Vasilenko T, Dall’Acqua S, Nagy M                and Mučaji P: Agrimonia eupatoria L. and Cynara cardunculus L. water infusions: phenolic profile and comparison                of   antioxidant   activities.   Molecules   20(11):   20538-20550,   2015.   PMID:   26593896.   DOI:                10.3390/molecules201119715                                                                                                                19","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL14                      Natural adenines exhibit an incredible range of biological activities                                                    1,3                                   1,                                                                                                   2                                                                      3                                                                                         2                   Miroslav Strnad *, Karel Doležal , Lucie Plíhalová , Gabriel Gonzáles , Jiří Grúz , Jiří                                        2                                   Voller , Lucie Rárová , Vladimír Kryštof , Radek Jorda 2                                                                          2                                                       2                   1  Laboratory of Growth Regulators, Palacký University \& Institute of Experimental Botany                                           ASCR, Šlechtitelů 27, 783 71 Olomouc                     2  Department of Experimental Biology, Palacký University, Šlechtitelů 27, Olomouc,                    3  Department of Chemical Biology, Palacký University, Šlechtitelů 27, Olomouc, Czech                                                         Republic                                       *Corresponding author: miroslav.strnad@upol.cz                 The purine ring system (imidazo[4,5-d]pyrimidine) is among the most ubiquitous of all the                heterocyclic compounds. Although purine itself has never been found in nature, substituted                purines like adenine and guanine or their respective nucleoside/nucleotide derivatives are the                most common class of nitrogen heterocycles which play  crucial roles in  a wide variety of                functions of living species. As nucleotides (AMP, GMP), they are the building blocks of nucleic                acids  (RNA/DNA).  They  serve  as  energy  cofactors  (ATP,  GTP),  as  part  of  coenzymes                (NAD/FAD)  in  oxidation-reduction  reactions,  as  important  second  messengers  in  many                intracellular  signal  transduction  processes  (cAMP/cGMP,  or  as  direct  neurotransmitters  by                binding to purinergic receptors (adenosine receptors). Therefore, it is not surprising that the                analogues of purines have found utility both as chemotherapeutics (antiviral, antibiotic, and                anticancer  agents)  and  pharmacodynamic  entities  (regulation  of  myocardial  oxygen                consumption and cardiac blood flow). Since the topic on adenine and its derivatives is obviously                too vast and broad  to cover it all in  a single talk, we will  mainly focus on  medicinal and                pharmaceutical  aspects  of  adenines  that  are  deemed  most  important  based  on  the  recent                scientific discoveries. Of particular interesting is their ability to protect cells against various                forms of stress and prevent some of the harmful effects of cell aging. For example, human skin                fibroblasts  cultured  in  the  presence  of  cytokinin  kinetin  or  trans-zeatin  retain  some                characteristics  of  cells  of  lower  passage.  Kinetin  is  even  able  to  increase  the  lifespan  of                invertebrates. The anti-proliferative activity of cytokinin ribosides (through induction of cell                cycle block or/and cell death) and bases (through induction of cell differentiation) has prompted                studies into their potential utility for the therapy of proliferative diseases like leukaemia, cancer                and  psoriasis.  Furthermore,  inhibitors  of  cyclin-dependent  kinases  olomoucine,  bohemine,                roscovitine, indirubin, etc., were inspired also by adenines. Particularly CDK5 inhibitors, as                                                                                      6                antiangiogenic  agents,  indicated  that  structure-activity  analyses  of  N -substituted  adenine                derivatives could greatly facilitate the identification of potent new antiangiogenic compounds.                In this presentation, the protective effects of phytohormones at molecular, cellular, tissue and                organismal  levels will be discussed. We would also like to discuss potential application of                phytohormones for the treatment of age-related diseases, including neurodegenerations.                  Acknowledgements: We wish to thank to Grant Agency of the Czech Republic, grant No. 23-                05474S.                                                                                                              20","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL15                            Antiadipogenic effects of coumarins from Citrus x limon                                                                                                 2                                                                             2                                      1,                                                       1                          Franz Bucar *, Lisa Raimann , Gabriele Schoiswohl , Alexander Kollau ,                                                          2                                                                             2                                             Bernd Mayer , Astrid Schrammel                   1 Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz,                                         Beethovenstrasse 8, A-8010 Graz, Austria                     2 Department of Pharmacology and Toxicology, Institute of Pharmaceutical Sciences,                               University of Graz, Humboldtstrasse 46, A-8010 Graz, Austria                                       *Corresponding author: franz.bucar@uni-graz.at                 The role of fat tissue as an endocrine organ, central to the biosynthesis of various hormones is                -  next to its close association to several metabolic diseases particularly in those suffering from                                                                     1                obesity- a focus for a large body of ongoing research . Given the various reported effects of                herbal substances on several organ systems, their effects on adipose tissue are a promising, yet                insufficiently explored area of research.                                                                                               2                For fermented products of lemon an anti-obesity effect could be shown previously . The aim of                this  study  was  to  characterize  the  impact  of  a  non-fermented  lemon  peel  extract  on                adipogenesis, to identify bioactive compounds and to clarify the underlying mechanism. The                study was performed with 3T3-L1 cells, which represent a well-established and widely used in                                                                3                vitro model to study aspects of adipocyte biology .                Applying a bioassay-guided approach, we identified coumarin derivatives in the flavedo layer                of  ripe  lemon  peels  that  reduced  3T3-L1  cell  differentiation.  LC-PDA-ESI-MS  analysis                revealed one of the active compounds as bergamottin whereas limettin was less potent.                Moreover, we shed light on the mechanism underlying the observed antiadipogenic effect.                  Acknowledgements: The authors thank Heike Stessel and Evamaria Imp for helpful technical                assistance  and  aws  (Austria  Wirtschaftservice  GmbH,  grant  no.  P2389315)  for  financial                support.                 References:                  1.     Giroud,  M.;  Jodeleit,  H.;  Prentice,  K.J.;  Bartelt,  A.  Adipocyte  function  and  the  development  of                cardiometabolic disease. J. Physiol. 2022, 600, 1189-1208. https://doi.org/10.1113/JP281979                2.     Wu, C.-C.; Huang, Y.-W.; Hou, C.-Y.; Chen, Y.-T.; Dong, C.-D.; Chen, C.-W.; Singhania, R.-R.; Leang,                J.-Y.; Hsieh, S.-L. The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat                Model with High-Calorie Diet-Induced Obesity. Nutrients 2021, 13, 2809. https://doi.org/10.3390/nu13082809                3.     Mussbacher, M.; Stessel, H.; Pirker, T.; Gorren, A. C. F.; Mayer, B.; Schrammel, A. S-nitrosoglutathione                inhibits adipogenesis in 3T3-L1 preadipocytes by S-nitrosation of CCAAT/enhancer-binding protein beta. Sci.                Rep. 2019, 9, 15403. https://doi.org/10.1038/s41598-019-51579-x                                                                                                                     21","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL16                Enhancing oligosaccharide characterization: Leveraging the power of NMR                                                      spectroscopy                                                                     1,                                                      Szabolcs Béni *                      1  Department of Analytical Chemistry, Institute of Chemistry, Eötvös Loránd University, 1117                                                     Budapest, Hungary                                      *Corresponding author: szabolcs.beni@ttk.elte.hu                  The growing interest in diverse carbohydrates stems from their multitude of beneficial health                properties, biocompatibility and widespread availability. However, within the realm of natural                products, carbohydrates present notable challenges in characterization due to their complex                structures. Consequently, the food and pharmaceutical industries require rapid and reliable yet                non-destructive analytical techniques for effectively assessing their structures.                NMR spectroscopy stands out as an effective method for rapid and non-destructive analysis,                offering detailed and direct structural insights into samples, enabling the simultaneous detection                and  quantification  of  byproducts,  impurities,  and  residual  reagents.  Moreover,  NMR                spectroscopy, despite being categorized as a relatively insensitive technique, furnishes intricate                structural  details,  especially  beneficial  in  detecting  subtle  differences  among  isomeric                carbohydrates. Its ability to provide the most comprehensive structural information underscores                its significance in analytical chemistry.                This  presentation  will  showcase  ongoing  projects  in  our  lab,  focusing  on  using  NMR  for                characterizing  bioactive  oligosaccharides.  We  will  discuss  the  challenges  in  characterizing                microheterogeneous glycosaminoglycans and delve into their detailed structural analysis during                degradation. Additionally, we present a straightforward and non-destructive NMR method for                determining the substitution pattern of 2-hydroxypropyl-β-cyclodextrin, a key pharmaceutical                excipient oligosaccharide. Lastly, we aim to highlight the utility of NMR in characterizing                human  milk  oligosaccharides,  emphasizing  its  extensive  use  in  distinguishing  isomeric                                                 1                structures through techniques like  H- N HSQC NMR.                                                     15                Acknowledgements: This project has received funding from the European Union’s Horizon                Europe  research  and  innovation  programme  under  the  Marie  Sklodowska-Curie  grant                agreement Bicyclos N° 101130235.                 References:                1.     Várnai,  B.;  Grabarics,  M.;  Szakács,  Z.;  Pagel,  K.;  Malanga,  M.;  Sohajda,  T.;  Béni,  S.  Structural                Characterization of Fondaparinux Interaction with Per-6-Amino-Beta-Cyclodextrin: An NMR and MS Study. J.                Pharm. Biomed. Anal. 2021, 197, 113947. https://doi.org/10.1016/j.jpba.2021.113947.                2.     Kalydi, E.; Malanga, M.; Nielsen T. T.; Wimmer, R.; Béni, S. Solving the puzzle of 2-hydroxypropyl β-                cyclodextrin: Detailed assignment of the substituent distribution by NMR spectroscopy Carb. Polym. 2024, in                press. https://doi.org/10.1016/j.carbpol.2024.122167.                                                                                 1                                                                                    15                3.     Garádi,  Z.;  Tóth,  A.;  Gáti,  T.;  Dancsó,  A.;  Béni,  S.  Utilizing  the  H- N  NMR  Methods  for  the                Characterization  of  Isomeric  Human  Milk  Oligosaccharides.  Int.  J.  Mol.  Sci.  2023,  24  (3),  2180.                https://doi.org/10.3390/ijms24032180.                                                                                                              22","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL17                   Integration of liquid-liquid chromatography in natural product research                                                       Simon Vlad Luca*                   Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, 85354                                                    Freising, Germany                                          *Corresponding author: vlad.luca@tum.de                  Liquid-liquid  chromatography  (LLC),  commonly  referred  to  as  countercurrent  separation                (CCS) or countercurrent distribution (CCD), is a solid-support-free preparative technique that                uses the two liquid phases of a pre-equilibrated biphasic system composed of two or more                solvents [1]. One of the liquid phases is maintained stationary inside the column with the help                of a centrifugal field, while the other (mobile phase) is passed through it. Due  to its high                adaptability,  versatility,  and  unique  features,  LLC  can  successfully  separate  complex                metabolomic mixtures, such as plant extracts. The flow direction and role of the phases can be                easily changed during operation, allowing for the implementation of various operating modes.                Some of these modes are unique to LLC and are employed to enhance the separation resolution                and  productivity  or  reduce  the  processing  time  and  solvent  consumption  [2].  The  almost                limitless variety of biphasic solvent combinations allows users to design a biphasic system                tailored to a particular separation. LLC has been shown to be helpful for the purification of                >2,500 natural products (NPs) with diverse chemical scaffolds [3]. As applications, numerous                examples in which LLC was integrated as a complementary platform preceding the evaluation                of  the  pharmacological  activity  of  NPs  will  be  presented.  For  instance,  the  separation  of                cannabinoids  with  cosmeceutical  properties,  the  fractionation  of  coumarins  with  central                nervous  system  modulatory  and  trypanocidal  effects,  the  purification  of  lignans  with  anti-                Trychophyton effects, or the isolation of piperamides with anti-Helicobacter activity will be                discussed.                  References:                 1.     Pauli, G.F. Pro, S.M., Friesen J.B., Countercurrent separation of natural products, J. Nat. Prod. 2008, 71,                1489-1508.                2.     Morley, R. Minceva, M. Operating mode and parameter selection in liquid-liquid chromatography, J.                Chromatogr. A 2020, 1617, 21.                3.     Skalicka-Woźniak, K., Garrard, I., A comprehensive classification of solvent systems used for natural                product purifications in countercurrent and centrifugal partition chromatography, Nat. Prod. Rep. 2015, 32, 1556-                1561.                                                                                                                        23","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL18                       Essentials in the acquisition, interpretation, and reporting of plant                                                   metabolite profiles                                                                     1,                                                    Christian Zidorn *                     1  Pharmazeutisches Institut, Abteilung Pharmazeutische Biologie, Christian-Albrechts-                               Universität zu Kiel, Gutenbergstraße 76, 24118 Kiel, Germany                                   *Corresponding author: czidorn@pharmazie.uni-kiel.de                  Plant metabolite profiling reveals the diversity of secondary or specialized metabolites in the                plant  kingdom.  Specialized  plant  metabolites  constitute  a  vast  class  of  chemicals  posing                significant challenges in analytical chemistry. In order to be of maximum scientific relevance,                scientific reports in this field must be transparent, make use of standards and reference materials                [1], and be based on correctly and traceably identified plant material [2]. This includes: (i)                critical review of previous literature and a reasoned sampling strategy; (ii) transparent plant                sampling with material documented by vouchers in public herbaria and, optimally, seed banks;                (iii)  if  possible,  inclusion  of  generally  available  reference  plant  material;  (iv)  transparent,                documented state-of-the art chemical analysis, ideally including chemical reference standards;                (v)  testing  for  artefacts  during  preparative  extraction  and  isolation,  using  gentle  analytical                methods;  (vi)  careful  chemical  data  interpretation,  and  (vii)  taking  all  previous  scientific                knowledge  into  account  in  reporting  the  scientific  data.  From  the  current  stage  of  the                phytochemical literature, selected comments and suggestions are given. In the past, proposed                revisions of botanical taxonomy were sometimes based on metabolite profiles, but this approach                (“chemosystematics” or “chemotaxonomy”) is outdated due to the advent of DNA sequence-                based phylogenies. In contrast, systematic comparisons of plant metabolite profiles in a known                phylogenetic framework remain relevant. This approach, known as chemophenetics [3], allows                characterizing  species  and  clades  based  on  their  array  of  specialized  metabolites,  aids  in                deducing the evolution of biosynthetic pathways and coevolution, and can serve in identifying                new sources of rare and economically interesting natural products.                 Acknowledgements: Many thanks are due to Niels Agerbirk (Copenhagen), Serhat Sezai Çiçek                (Hamburg), Franziska S. Hanschen (Großbeeren), and Alfonso Mangoni (Naples), who were                my invaluable co-authors of the publication, this presentation is based upon.                 References:                1.     Çiçek,  S.S.,  Mangoni,  A.,  Hanschen,  F.S.,  Agerbirk,  N.,  Zidorn,  C.  Essentials  in  the  acquisition,                interpretation,  and  reporting  of  plant  metabolite  profiles.  Phytochemistry  2024,  220,  114004.                doi:10.1016/j.phytochem.2024.114004                2.     Zidorn, C. Guidelines for consistent characterisation and documentation of plant source materials for                studies   in   phytochemistry   and   phytopharmacology.   Phytochemistry   2017,   139,   56-59.                doi:10.1016/j.phytochem.2017.04.004                3.     Zidorn, C. Plant chemophenetics − a new term for plant chemosystematics/plant chemotaxonomy in the                macro-molecular era. Phytochemistry 2019, 163, 147-148. doi:10.1016/j.phytochem.2019.02.013                                                                                                               24","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL19                      Quality, safety and efficacy of natural health product – how HPTLC,                                    bioassays, and KI can contribute to evidence                                                                     1,2,                                                    Evelyn Wolfram *                   1  Zürich University of Applied Sciences, Institute for Chemistry and Biotechnology, Natural                      Products and Phytopharmacy Research Group, CH-8820 Wädenswil, Switzerland                               2  Planar4 GmbH, CH-8712 Stäfa, Switzerland, info@planar4.ch                                      *Corresponding author: evelyn.wolfram@zhaw.ch                 In recent years quicker and more thorough examination of natural products and their intricate                compositions  sourced  from  nature  have  been  enabled  by  modern  Phytochemical Analysis                techniques like Liquid-Column and Gas Chromatography coupled with MS, along with NMR.                The aims are still quantification of secondary metabolic analysis and standardization marker                quantitation. Yet, even amidst these advancements, the simplicity of planar chromatography                remains vital. Modernized into High Performance Thin Layer Chromatography (HPTLC), it                retains its importance in pharmaceutical quality control, especially in Ph Eur monographs where                it verifies plant material authenticity. However, HPTLC offers more than just quality control—                it serves as both a planar solid phase extraction and fractionation tool  in a single step. Its                                                                                           1                versatility extends to integration with MS or bioassays, such as bioautography , also known as                \"effect-directed analysis.\" Planar Mutagenicity assessment, and anti-fungal efficacy screening,                are  offering  valuable  insights  and  showcase  its  multifaceted  applications  in  assessing  the                quality, safety, and efficacy of natural health products.                Moreover, when considering the quality, safety, and efficacy of natural health products, the                combined  application  of  Phytochemical  Analysis  and  computational  tools  and  artificial                intelligence (AI) for natural products can significantly contribute to building robust evidence.                                              2                Computational  methodologies   ensure  that  data  from  various  sources  are  synthesized                effectively, enhancing the overall evaluation of product quality and safety and computational                literature mining tools  offer efficacy data of single compounds as well as extracts.                                      3                 Acknowledgements: The authors thank the organizers for the invitation and all collaborators                that significantly contributed to the experiences and data presented in this overview lecture                  References:                1.     Bräm,  S  and  Wolfram,  E.  Recent  advances  in  effect-directed  enzyme  assays  based  on  thin-layer                chromatography. Phytochemical Analysis. 2017, 28, 74-86. https://doi.org/10.1002/pca.2669                2.     Wolfram E and Trifan A. Computational aids for assessing bioactivities. Book Chapter 11 In: Sarker S                and Nahar L (Eds.) Computational Phytochemistry. Elsevier Second Edition 2024, ISBN 978-0-443-16102-5.                https://doi.org/10.1016/B978-0-443-16102-5.00005-5                3.     Lardos, A, Aghaebrahimian, A,  Koroleva, A,  Sidorova,  J,  Wolfram,  E, Anisimova,  M  and  Gil,  M.                Computational Literature-based Discovery for Natural Products Research: Current State and Future Prospects.                Frontiers in Bioinformatics 2022, 2:827207. https://doi.org/10.3389/fbinf.2022.827207                                                                                                                25","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL20 Dra. Mariola Macías Award                     Taking Advantage of Allelopathy: Discovery of Natural Products and                        Design of New Strigolactone Analogues for Weed Management                                                           1                                                                       1                                                                                                    3                        Jesús G. Zorrilla  1,2, *, Antonio Cala , Carlos Rial , Mónica Fernández-Aparicio , Rosa                                 1                                                                                        2                                                                     2                                                       1                        M. Varela , José M. G. Molinillo , Marco Masi , Alessio Cimmino , Antonio Moreno-                                                         3                                                   Robles , Francisco A. Macías                                                                               1                        1  University of Cadiz, Organic Chemistry Department, Facultad de Ciencias, Institute                           of Biomolecules, C/Avenida República Saharaui, s/n, 11510 Puerto Real, Spain                           2  University of Naples Federico II, Chemical Sciences Department, Complesso                                   Universitario Monte S. Angelo, Via Cintia, 80126 Naples, Italy                           3  Institute for Sustainable Agriculture (IAS), CSIC, Plant Breeding Department,                                        Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain                                            *Corresponding author: jesus.zorrilla@uca.es                 Parasitic weeds pose a threat to both crops and ornamental plants. Ineffective control can lead                to substantial crop losses, highlighting the need for  suitable management strategies. Recent                research has notably focused on the species of Orobanche, Phelipanche and Cuscuta genera,                which represent ongoing challenges in agronomy due to their persistent seedbank, the difficulty                of controlling them after crop infection, and their capacity to evolve new biotypes that expand                host species range and/or overcome existing crop resistance. Since agrochemicals based on                allelochemicals  are  promising  materials  for  sustainable  weed  control,  a  key  approach  is                discovering accessible compounds capable of stimulating or inhibiting seed germination and                seedling growth. Costunolide, a sesquiterpene lactone found in certain plants, presents a unique                opportunity for designing, via semi-synthesis, bioactive compounds that emulate the activity of                some phytohormones naturally influencing parasitic germination in ecosystems. Following this                approach, various collections of strigolactone analogues and mimics were synthesized, which                were active in bioassays against parasitic weeds. Their isomeric features led to the discussion                of structure-activity relationships, being compared to those of natural strigolactones. Seeking                to describe novel allelochemicals with activity on parasitic germination, subsequent screening                studies of diverse chemical structures have yielded promising results, notably with compounds                such as 2-benzoxazolinone, hydrocinnamic acid, and caffeic acid. Besides, new natural sources                of bioactive compounds have been searched and identified, including extracts from safflower                (Carthamus tinctorius) and Centaurea cineraria, which contained active strigolactones and                sesquiterpene lactones. This presentation highlights the approaches of long-term research aimed                at developing sustainable solutions for parasitic weeds, also helping to describe new models for                the development of future agrochemicals based on natural products.Acknowledgements: Work                cofunded  by  PID2020-115747RB-I00,  PID2020-114668RB-I00,  and  RYC-2015-18961                projects,  and  Plan  Propio-UCA,  call  “Investigadores  Noveles,  Proyectos  para  impulsar  su                Carrera Científica” (PR2023-026). J.G.Z. thanks Univ. of Cadiz for Margarita Salas support                (2021-067/PN/MS-RECUAL/CD), funded by the NextGenerationEU program of the European                Union. A.M.R. thanks JAE-Intro ICU (JAEIntro2021-5-IAS-02), CSIC.                                                                                                                 26","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL21                  CYP1-catalysed bio-activation of the polymethoxy flavone chrysosplenetin                                                                                                   1,                                                            2                                                  1                       Gael Noel Neh Neba Ambe , Suat Sari , Didem Şöhretoğlu , Randolph Arroo *                                                                                2                                   1  De Montfort University, Leicester School of Pharmacy,                                           The Gateway, Leicester LE1 9BH, UK                                        2  Hacettepe University, Faculty of Pharmacy,                                               06100 Sıhhiye Ankara, Türkiye                                        *Corresponding author: rrjarroo@dmu.ac.uk                  The potential role of flavonoids, notably polyhydroxy flavones and flavonols, was confirmed                in  numerous  in  vitro  assays,  where  these  compounds  showed  anti-proliferative  and  anti-                inflammatory activity.                 Polymethoxy flavones generally show less activity in  in vitro tests. However,  in vivo, they                remain in systemic circulation for a prolonged period. In pharmacological terms, their ‘area                under the curve’ or AUC is larger, which means that their clearance rate from the body is slower                and the extent of exposure to these compounds is higher.                 Previous work in our group has identified a range of polymethoxy flavones that are substrates                for the dealkylating enzymes CYP1A1 and CYP1B1. These two enzymes are over-expressed                in a range of tumour cell lines. This means that dietary methoxy flavones can circulate in the                human body for a prolonged period, causing little overall effect since they have low biological                activity. However, only in selected tumour cells, the polymethoxy flavones are converted into                polyhydroxy flavones – significantly increasing their anti-proliferative activity.                 The  polymethoxy  flavone  chrysosplenetin,  a  by-product  of  artemisinin  extraction  from                Artemisia annua L., was shown to selectively inhibit the proliferation of CYP-1 expressing                tumour cell lines. Our data indicate that chrysosplenetin itself shows little bioactivity, but the                CYP1-catalysed conversion products have anti-proliferative activity.                                                                                                                                    27","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL22                         The challenges in the study of bioactive natural products using                                       comprehensive analytical approaches                                                                                1                                            Stefano Dall’Acqua *, Stefania Sut                                                               1,                     1  Natural Product Lab, Department of Pharmaceutical and Pharmacological Sciences                                  University of Padova, via Marzolo 5, 35121 Padova, Italy                                     *Corresponding author: stefano.dallacqua@unipd.it                    The study of natural compounds from plants in the field of health and medicinal chemistry is                full of successful examples leading to the development of many drugs as morphine, digitoxin,                                   1                quinine  and  Taxol.   Furthermore,  in  the  last  years  plant  have  been  studied  as  functional                ingredients for the production of nutraceuticals and herbal medicine. The research in this field                have strongly evolved thanks to the technical improvement of chromatographic approaches,                and  the  larger  availability  and  easy  of  use  of  Nuclear  Magnetic  Resonance  and  Mass                Spectrometry the application of metabolomics. But, the challenges in this field are still many.                Isolation of new compounds, solubility and stability issues of purified compounds, availability                of plant material for the extraction and purification processes are some of the “traditional”                challenges  in  this  field  of  research.  The  large  diffusion  of  HR-MS  spectroscopy  and  the                improvement of software give new emphasis to analytical tools with the opportunity to perform                dereplication studies in attempt to select more promising plant sources and the possible new                compounds. In this talk the need of comprehensive approach combining different techniques                comprising  High-  and  Low-resolution  Mass  Spectrometry   as  well  as  Nuclear  Magnetic                                                                           2                Resonances, bioactivity assays and multivariate data analysis will be described showing the                importance in the study of natural bioactive compounds of comprehensive and multidisciplinary                approaches.                  References:                 1.     David, J. Newman and Gordon, M. Cragg. Natural Products as Sources of New Drugs over the Nearly                Four    Decades   from    01/1981   to   09/2019. J.   Nat.   Prod. 2020,   83,   3,   770–803.                https://doi.org/10.1021/acs.jnatprod.9b01285                2.     Raffaelli, A. and Saba, A. Ion scanning or ion trapping: Why not both? Mass Spec Rev. 2023; 42, 1152–                1173. https:// 10.1002/mas.21746                                                                                                                           28","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL23                     Semisynthetic derivatives of galanthamine and their biological activity                 Lucie Cahlíková *, Abdullah Al Mamun , Ondřej Janďourek , Filip Pidaný , Eman Khaled ,                                                        1                                                                                                        1,3                                1,                                                                                         1                                                                           2                                                      Radim Havelek  4                  1 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles                                                University, Czech Republic                 2 Department of Biological and Medical Sciences, Faculty of Pharmacy, Charles University,                                 Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic                3 Applied Chemistry Department, Chemical Industries Institute, National Research Centre, El-                                         Buhouth st., Dokki, p.o. 12622, Giza, Egypt                 4 Department of Biological and Medical Sciences, Faculty of Pharmacy, Charles University,                                 Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic                                        *Corresponding author: cahlikova@faf.cuni.cz                  Galanthamine is a tertiary isoquinoline alkaloid isolated from several plants in the plant family                Amaryllidaceae. The idea for developing a medical product from these species seems to be                based on the local use of one of these species in a remote part of Europe. Today, galanthamine,                especially under its brand names Reminyl® and Nivalin®, is commonly used in the treatment                of Alzheimer's disease (AD) as a reversible competitive inhibitor of acetylcholinesterase. Its                additional activity, the allosteric modulation of nicotinic ACh receptors, is responsible for its                therapeutic benefit in AD.                Since  this  alkaloid  has  been  isolated  in  gram  amounts  from  plant  material  (Narcissus                pseudonarcissus cv. Carlton), it has been used for the preparation of semisynthetic derivatives.                The  semisynthetic  derivatives  of  galanthamine  were  screened  for  their  in  vitro                antimycobacterial activity against Mycobacterium tuberculosis H37Ra and other mycobacterial                strains (M. aurum, M. avium, M. smegmatis, and M. kansasii). The most active compounds                were also studied for their in vitro hepatotoxicity on the hepatocellular carcinoma cell line                HepG2. In general, the derivatization of galanthamine was associated with a significant increase                in antimycobacterial activity.                Moreover, newly developed derivatives were also screened on a panel of cancerous and non-                cancerous cell lines. Some of the derivatives showed promising cytotoxic activity against tested                cell lines.                 Acknowledgements: Current study has been supported by the project New Technologies for                Translational  Research  in  Pharmaceutical  Sciences  /NETPHARM,  project  ID                CZ.02.01.01/00/22_008/0004607, is co-funded by the European Union.                 References:                1.  Global tuberculosis report 2022, Global Tuberculosis Report 2022 (who.int)                                                                                                                   29","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PL24                    Corn-Cockle phytochemical cocktail – How to make good stuff from old                                                          weed?                                                      Adam Matkowski*                       Botanical Garden of Medicinal Plants, Department of Pharmaceutical Biology and                                    Biotechnology, Wroclaw Medical University, Poland                                   *Corresponding author: pharmaceutical.biology@wp.eu                  Plants from the Caryophyllaceae family are widely known for their saponin content. Many of                them are also abundant in type 1 ribosome-inactivating proteins (RIP) that are postulated to act                in concert with certain saponins in boosting the RIPs toxicity by facilitating cell entry.                For example, an ancient and almost forgotten annual weed  - Agrostemma githago L., once                notorious for contaminating cereal grains, stands out for its notable combination of RIP and                triterpenoid glycosides. Nowadays, modern weeding practices have nearly eradicated this plant,                to the point where it is now considered endangered.                In addition to the well-known triterpenoid saponins and RIP, there is a high content of flavonoid                C-glycosides, such as orientin that may render interesting pharmacological properties beyond                the saponin/RIP related toxicity.                In history, some records were known on using A. githago in both official and folk medicine, but                all are scarce and lack coherent information. Currently, the seeds are being marketed for use in                blooming meadows in the urban environments.                In this paper, the state-of-the-art in corn-cockle phytochemistry will be presented as well as                some  outlooks  into  using  biotechnological  approach  to  understand  the  mechanisms  of                production and regulation of the major classes of phytochemicals and bioactive proteins. This                direction may put the corn-cockle back on stage in pharmaceutical applications.                 Acknowledgements: Funding of the project: National Science Center of Poland (NCN), grant                no. 2020/39/I/NZ7/01515 (RIPSAPO). I would also like to honor all partners and collaborators                in  the  project:  Monika  Bielecka,  Fabian  Bülow,  Weronika  Kozłowska,  Weronika                Korzeniowska,  Iza  Nawrot-Hadzik,  Alexander  Smakosz,  Marta  Stafiniak,  Marcin  Surma,                Marek  Surma,  Alexander  Weng,  Daria  Zblewska,  Sylwia  Zielińska  as  well  as  Reneta                Gevrenova, Łukasz Pecio, Solomija Pecio.                                                                                                                              30","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                     List of Oral Presentations (Listed according to appearance in program)                 OL1     Kateřina Dadáková                         Lignans in wine                 OL2     Jonas Stehlin                         The Receptarium of Burkhard from Hallwyl – from digital indexing historical                         recipes to pharmacological screening of plant ingredients                 OL3     Magdalena Grajzer                         Unlocking the powers of Fragaria vesca: A revelatory dive into the impact of                         extraction techniques on seed oil potency and applications                 OL4     Ernesto Gargiulo                         Phytochemical investigation and TRPA1/TRPM8 modulation profile of the                         cannabigerol-rich Cannabis sativa L. Chemotype IV                 OL5     Veronika Stoilkovska Gjorgievska                         Unlocking the potential of Macedonian Cannabis wild-types: phytochemical                         profiling and UPOV descriptors assessment                 OL6     Francesca Guzzo                         New steroidal saponins from leaves of Agave bracteosa: structural                         characterization and cytotoxic activity                 OL7     Chao Zhang                         In situ characterisation of plant defence phytohormones and related metabolites                         via advanced mass spectrometry imaging                 OL8     Jan Günther                         Plant triterpenoid saponins – from structure to bioactivity                 OL9     Hafize Yuca                         In vitro antidiabetic and anticholinesterase potentials of eleven medicinal plants                         from Lamiaceae naturally grown in Turkey                 OL10  Sylvia Kalli                         Cellular responses and targets in food-spoilage yeasts exposed to antifungal                         prenylated isoflavonoids                 OL11  Adam Junka                         Integration of plant-based antimicrobials and magnetic fields for enhanced                         penetration of pathogenic biofilms                 OL12  Selen İlgün                         Evaluation of the chemical content and biological activities of “Orobanche                         ramosa” L.                 OL13  Katerina Tomisova                         The interaction of silymarin with the gut microbiota and its differences with age                 OL14  Valeria Iobbi                         Characterization and sustainable agrochemical application of Vessalico garlic                         ecotype                 OL15  Iasmina Honceriu                         Anti-Alzheimer effects of Paradol, mansorin A, and mansonone G in a zebrafish                         model of Alzheimer’s disease induced with okadaic acid                 OL16  Radim Havelek                         The antiproliferative activity and apoptosis-inducing mechanism of the                         Amaryllidaceae alkaloid montanine on A549 and MOLT-4 human cancer cells                 OL17  Melek Karaaslan                         Analgesic and anti-inflammatory activity of some Glaucium taxons                 OL18  Adrian H. Kopf                         Valorisation of North Atlantic macroalgae: Antimicrobial activity of phlorotannins                                                                                                            31","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 OL19  Shamee Bhattacharjee                         Spice-derived bioactive components inhibit cancer metastasis by targeting cellular                         signaling pathways                 OL20  Janniek Ritsema                         Antibacterial prenylated (iso)flavonoids from Fabaceae: inhibition and                         permeabilization of methicillin-resistant Staphylococcus aureus                 OL21  Jana Křoustková                         Carltonines from Narcissus pseudonarcissus cv. Carlton and lead optimization                 OL22  Chidoh Kootlole                         NMR-based metabolomics and anti-leukemic activity of plants used in traditional                         medicine in Botswana                 OL23  Chiara Collevecchio                         Selective isolation and concentration of oleuropein and elenolic acid from Olea                         europaea L. aqueous leaves extracts                 OL24  Hasan Kırmızıbekmez                         Unprecedented phenolic compounds from Prospero autumnale (L.) Speta with in                         vitro cytotoxic activities                 OL25  Nisa Beril Sen                         Comparative phytochemical analysis and bioactivity evaluation of Castanea sativa,                         Salix and Quercus spp. flower pollen and their bee pollen                 OL26  Livia Reguengo                         Anti-inflammatory properties of jabuticaba peel bioactive compounds during in                         vitro gastrointestinal and colonic digestion                 OL27  Sylwester Ślusarczyk                         Pulse Electric Field (PEF) treatment as innovative approach for milking the plant                         roots; advantage and disadvantage of this technique                 OL28  Augustin Bildstein                         Originality of wild hops from northern France - a chemosensory approach                 OL29  Kajetan Grzelka                         Application of Pulsed Electric Fields increases yield of flavonoids and enables                         their extraction from Scutellaria baicalensis Georgi living roots                 OL30  Cristina Terenzi                         Valorization of legume by-products for pharmaceutical, cosmetic, and                         nutraceutical applications                 OL31  Maria João Rodrigues                         Establishment and elicitation of Limonium algarvense Erben callus cultures:                         Implications for primary and secondary metabolism                 OL32  Senthil Kumar Kanthasamy Jayabal                         Cosmetic applications of essential oils extracted from indigenous forest species of                         Taiwan                 OL33  Jonas Kappen                         Chasing molecules - a structure elucidation journey                 OL34  Fabian Alperth                         Extraction of Spilanthol from Acmella oleracea with Natural Deep Eutectic                         Solvents                 OL35  Ceren Öztürk                         Two new anthraquinones and other secondary metabolites from Asperula sintenisii                         Achers ex Bornm                                                                                                                  32","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 OL36  Vahap Murat Kutluay                         Integrating network pharmacology and experimental validation: In vitro                         assessment of neuroprotective effects and enzyme inhibition studies on Prunella                         vulgaris L. extract and isolated compounds                 OL37  Micol Caichiolo                         Anti-inflammatory properties of biomolecules from the Euganean thermal district                 OL38  Derya Çiçek Polat                         Antioxidant activity and phenolic compounds contents of Tribulus terrestris L.                                                                                                                                                                                    33","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL1                                                     Lignans in wine                                                         1,                                                                                      2                                                                      2                                    Kateřina Dadáková *, Miloš Vidlář , Mojmír Baroň                  1  Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625                                                  00 Brno, Czech Republic                 2  Department of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno,                                       Valtická 337, 691 44 Lednice, Czech Republic                                      *Corresponding author: k.dadakova@mail.muni.cz                 In the plant kingdom, a large number of different specialized metabolites can be found that help                the plant in its interaction with the environment. These specialized metabolites include lignans,                which serve as protection for the plant against pathogenic microorganisms and herbivores. In                humans, lignans are converted to enterolignans after ingestion. Both lignans and enterolignans                exhibit antimicrobial, anti-inflammatory, antioxidant, and estrogen-like activities. One source                of  lignans  in  the  human  diet  is  wine,  where,  depending  on  the  particular  type  of  wine,                lyoniresinol,  isolariciresinol,  secoisolariciresinol,  and  to  a  lesser  extent  syringaresinol,                matairesinol and lariciresinol may be present. To investigate the possibilities of influencing the                lignan concentrations in wine, we validated an LC-MS method for the determination of lignans                                  1                in wine and grapes . Our results show that the content of lignans in wine depends on the variety                and vintage, but it can also be increased by using different maturation methods. In concrete, the                content of lyoniresinol and syringaresinol depends on the barrel used for wine maturation, while                the content of isolariciresinol and secoisolariciresinol can be increased by maturation in contact                with the grape seeds and skins . Consequently, wines richer in lignans may provide health                                               1,2                benefits associated with the intake of these plant metabolites.                 Acknowledgements:  Authors  thank  to  Internal  Grant  Agency  of  MENDELU  Faculty  of                Horticulture,  IGA-ZF/2021-SI2009,  Study  of  polyphenolics  compounds  in  wines  and  parts                vines; Ministry of Education Youth and Sports, CZ.02.1.01/0.0/0.0/l6_0l7/0002334, Research                Infrastructure for Young Scientists; Grant Agency of Masaryk University, MUNI/A/1604/2020,                Support for biochemical research in 2021.                 References:                1.     Dadáková, K.; Jurasová, L.; Kašparovský, T.; Průšová, B.; Baroň, M.; Sochor, J. Origin of Wine Lignans.                Plant Foods Hum Nutr 2021, 76 (4), 472–477. https://doi.org/10.1007/s11130-021-00928-1.                2.     Vidlar, M.; Dadakova, K.; Kasparovsky, T.; Baron, M.; Průšová, B.; Sochor, J. Study of Lignans in Wine                Originating  from  Different  Types  of  Oak  Barrels.  OENO  One  2023,  57  (3),  233–242.                https://doi.org/10.20870/oeno-one.2023.57.3.7128.                                                                                                                      34","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL2                      The Receptarium of Burkhard from Hallwyl – from digital indexing                      historical recipes to pharmacological screening of plant ingredients                               1,                 Jonas Stehlin *, Ina Albert , Evelyn Wolfram , Thomas Frei , Barbara Frei Haller , Andreas                                                            1                                                                           3                                                                                               3,4                                           2                                                                1,3                                                         Lardos                      1  ZHAW Zurich University of Applied Sciences, School of Life Sciences and Facility                   Management, Institute of Chemistry and Biotechnology, Natural Product Chemistry and                         Phytopharmacy Group, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland                     2  ZHAW Zurich University of Applied Sciences, School of Life Sciences and Facility                   Management, Institute of Chemistry and Biotechnology, Pharmaceutical Technology and                          Pharmacology Group, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland                  3  Pro Thesauro Sanitatis (PTS), c/o Thomas Frei, Kappelen 20, 5706 Boniswil, Switzerland                4  ETH Zürich, Institute of Pharmaceutical Sciences (IPW), Vladimir-Prelog-Weg 1-5/10, 8093                                                    Zurich, Switzerland                                       *Corresponding author: jonasstehlin@gmail.com                 The ethnopharmacological analysis of historical texts offers promising opportunities for drug                discovery. Despite the vast amounts of knowledge contained in historical texts few studies have                attempted  to  transfer  this  information  into  a  modern  context.  In  this  work,  we  present  the                development of  a relational database for indexing  recipes contained in the Receptarium of                                                 th                Burkhard from Hallwyl (RBH) (16  century Switzerland) and the subsequent in vitro screening                of plants mentioned in this resource.                Recipes  in  RBH  directed  at  the  treatment  of  skin complaints  or  topically  manifest  disease                symptoms  were  recorded  in  a  specially  developed  relational  database  consisting  of  20                interlinked tables. Focusing on herbal recipes, we explored the mentioned plants and their uses                by  employing  specific  literature.  Botanical  identities  of  the  plant  names  mentioned  were                determined and ranked based on their plausibility of being the correct identification. Historical                plant uses were interpreted from a modern clinical perspective and the pharmacological basis                of the associated diseases elucidated.                The database allows for quantitative and qualitative analysis of individual recipe parameters                and targeted identification of candidate plants for screening campaigns. In order to assess the                potential  of  RBH  as  a  resource  for  pharmacological  investigations,  a  preliminary  in  vitro                screening was conducted. Eleven plants were selected the historical use of which seems to be                associated  with  inflammatory  conditions.  Hydroethanolic  extracts  were  then  tested  for                cytotoxicity in NIH-3T3 murine fibroblasts and modulation of LPS induced IL-6 and TNF                release in human whole blood and PBMC. Promising candidate plants were identified among                which  Vincetoxicum  hirundinaria  Medik.  stands  out,  with  the  ethanolic  macerate  strongly                inhibiting IL-6 release in PBMC (IC50 3.6 µg/mL).                Our work illustrates one possible approach to ethnopharmacological data mining of historical                texts.  We  hope  that  our  methodology  will  contribute  to  the  discussion  on  solutions  for                transferring premodern pharmaco-botanical information into a modern context.                                                                                                               35","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL3                   Unlocking the powers of Fragaria vesca: A revelatory dive into the impact                         of extraction techniques on seed oil potency and applications                                          1,                                                           2                                                                            3                                                                                             4                       Magdalena Grajzer *, Benita Wiatrak , Paulina Jawień , Łukasz Marczak , Anna                                               5                                4                                                                                    6                                                                                                      1                                                            5                    Wojakowska , Rafał Wiejak , Edward Rój , Wojciech Grzebieluch  and Anna Prescha                  1  Department of Dietetics and Food Science, Borowska 211, 50-556 Wroclaw, Department of                                                                                            2                Pharmacology, Mikulicza-Radeckiego; Wroclaw 50-345, Wroclaw Medical University, Poland                  3  Department of Biostructure and Animal Physiology, Wroclaw University of Environmental                                 and Life Sciences, Norwida 25/27, 50-375 Wroclaw, Poland                    4  European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry,                                        Polish Academy of Sciences, Poznań, Poland                    5  Łukasiewicz Research Network – New Chemical Syntheses Institute, Research Group                   Supercritical Extraction, Al. Tysiąclecia Państwa Polskiego 13a, 24-110 Puławy, Poland                   6  Department of Conservative Dentistry with Endodontics, Wroclaw Medical University,                                          Krakowska 26, 50-425 Wroclaw, Poland                                   *Corresponding author: magdalena.grajzer@umw.edu.pl                 Wild strawberry (Fragaria vesca L.) seed oil (WSO) recovered by two methods – cold pressing                (CP)  and  extraction  with  supercritical  carbon  dioxide  (SCO2E)  –  taking  into  account  the                different extraction time, was characterized for its composition and quality. The cytotoxicity                assessment of WSOs was also carried out using normal human dermal fibroblast (NHDF) cell                    1                line . Tocopherol, and total polyphenol contents were significantly higher in WSO recovered                by SCO2E, up to 1901.0, and 58.5 mg/kg, respectively in comparison with CP oil. In CP oil the                highest  content  of  carotenoids  and  squalene  was  determined  (123.8  and  31.4  mg/kg,                respectively).  Phytosterol  summed  up  to  5396  mg/kg  in  WSO  collected  in  30  minutes  of                SCO2E, moreover the highest oxidative stability was found for this oil. All studied WSOs were                non-cytotoxic in lactate dehydrogenase (LDH) leaching and sulforhodamine B (SRB) assays,                however oils collected by SCO2E in 15 and 30 minutes were found to be cytotoxic  in the                tetrazolium salt (MTT) test with the CC50 at the concentration of 3.4 and 5.5%, respectively. In                conclusion, the composition of WSO indicates that depending on the method of its recovery                from seeds can have different bio-potency and various applications.                 Acknowledgements:  Authors  thank  to  Wroclaw  Medical  University,  Grant  No.                SUBK.D040.22.030,  Bioaktywność  oleju  z  poziomki  (Fragaria vesca  L.)  i  tłoczonego  na                zimno   ekstrahowanego  nadkrytycznym  dwutlenkiem  węgla\"  and  National  Science  Center,                Grant  No.  2019/03/X/NZ9/00764[ MINI.D040.19.009]:”Charakterystyka  i  stabilność                oksydatywna oleju z nasion poziomek uzyskanego metodą ekstrakcji dwutlenkiem węgla w                warunkach nadkrytycznych”                 References:                1.  Grajzer,  M.; Wiatrak,  B.;  Gębarowski, T.;  Matkowski, A.;  Grajeta,  H.;  Rój,  E.;  Kulma, A.;  Prescha, A.                    Chemistry, oxidative stability and bioactivity of oil extracted from Rosa rugosa (Thunb.) seeds by supercritical                    carbon dioxide. Food Chem. 2021, 335, 127649, doi:10.1016/j.foodchem.2020.127649.                                                                                                               36","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL4                  Phytochemical investigation and TRPA1/TRPM8 modulation profile of the                               cannabigerol-rich Cannabis sativa L. Chemotype IV                                                                 2                                                                                                       2                                                                               3                                   1,                   Ernesto Gargiulo *, Aniello Schiano Moriello , Lolita Arnoldi , Luciano De Petrocellis ,                                                                 1                                              1                                                                                                 1                         Giuseppina Chianese , Rosa Maria Vitale , and Orazio Taglialatela-Scafati                 1  Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II,                                         Via D. Montesano 49, 80131 Napoli, Italy                   2  Institute of Biomolecular Chemistry, National Research Council (ICB-CNR), Via Campi                                           Flegrei 34, 80078, Pozzuoli (NA), Italy                             3  R\&D, Indena SpA, via Don Minzoni, 6 – 20049 Settala (MI), Italy                                      *Corresponding author: ernesto.gargiulo@unina.it                 Cannabis  sativa  L.  Chemotype  IV  derives  from  a  homozygotic  B0/B0  breed  of  cannabis                resulting in the presence of CBG/CBGA being the major phytocannabinoids (PCs), followed                                         1                by CBD/CBDA (<0.05%) . In our endeavour to explore lesser-known cannabis chemotypes ,                                                                                                         2                we undertook the first extensive phytochemical investigation of an industrial extract of C. sativa                chemotype IV. Our previously developed expeditious workflow for the phytochemical analysis                of cannabis extracts  allowed the isolation of 13 secondary metabolites, thereby enriching the                                   3                repertoire of the PCs chemical family, with a particular emphasis on the identification of minor                constituents pivotal for uncovering novel biological endpoints.                In addition to the expected CBGA/CBG, CBDA/CBD, nine new PCs were isolated and fully                characterized by HR-ESIMS and 1D and 2D NMR. Newly isolated PCs included mono- or                dihydroxylated CBGA/CBG analogs, a congener with a truncated side chain, cyclocannabigerol                B, and cannabifuranols A and B, characterized by unprecedented structural architecture within                PCs chemical family.                Isolated PCs were assayed on the receptor channels TRPA1 and TRPM8 unveiling a potent dual                TRPA1  agonist/TRPM8  antagonist  profile  for  some  compounds.  For  one  of  isolated                phytocannabinoids, chiral separation of the two enantiomers was carried out and resulted in the                discovery of a synergistic effect on TRPA1.                Acknowledgements: This research was funded by MUR, research grant PRIN2017, Project                WN73PL (Bioactivity-directed exploration of the phytocannabinoid chemical space).                 References:                1.  de Meijer, E. P. M.; Hammond, K. M. Euphytica. 2005, 145, 189−198. https://doi.org/10.1007/s10681-005-                    1164-8.                2.  Salamone, S.; Waltl, L.; Pompignan, A.; Grassi, G.; Chianese, G.; Koeberle, A.; Pollastro, F. Plants. 2022, 11                    (16). https://doi.org/10.3390/plants11162130.                3.  Chianese, G.; Sirignano, C.; Benetti, E.; Marzaroli, V.; Collado, J. A.; De La Vega, L.; Appendino, G.; Muñoz,                    E.; Taglialatela-Scafati, O.  J. Nat. Prod. 2022, 1089–1097. https://doi.org/10.1021/acs.jnatprod.1c01198.                                                                                                                 37","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL5                 Unlocking the potential of Macedonian Cannabis wild-types: phytochemical                                    profiling and UPOV descriptors assessment                      Veronika Stoilkovska Gjorgievska*, Ivana Cvetkovikj Karanfilova, Ana Trajkovska,                         Iskra Davkova, Marija Karapandzova, Svetlana Kulevanova, Gjoshe Stefkov                  Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University, Majka                                    Tereza 47, 1000 Skopje, Republic of North Macedonia                                    *Corresponding author: vsgjorgievska@ff.ukim.edu.mk                 The EU Plant Variety Database lists 177 varieties of Cannabis sativa L. (hemp) so far. Wild                growing Cannabis plants have been detected in Macedonia but their origin remains uncertain                in  terms  of  their  origin  and  classification  as  autochthonous,  domesticated,  or  remnants  of                cultivated populations.                Our study aimed to evaluate the distinctiveness of Macedonian wild-growing Cannabis plants                by conducting morphological and phytochemical characterization. Plants were collected in seed                maturation  stage  from  two  areas  in  Eastern  Macedonia  and  seeds  were  obtained.  After                successful germination with scarification method, they were outdoor cultivated, along with four                commercial Cannabis strains (high CBD (Charlotte’s Angel), high Δ9-THC (Bubba Kush x OG                Kush),  and  two  50:50  CBD:Δ9-THC  strains).  Morphological  assessment  based  on  the                International  Union  for  the  Protection  of  New Varieties  of  Plants  (UPOV)  descriptors  was                conducted for all cultivated Cannabis samples. Additionally, germination, growth and flower                yield  were  accessed.  Phytochemical  analysis  on  cannabinoids  followed  the  German                pharmacopoeial monograph for Cannabis inflorescence.                All commercial strains' seeds showed 100% germinability, whereas wild-types germination                rates varied from 65% to 20%. The highest flower yield mass from a single plant was obtained                from Charlotte’s Angel (128.5 g), while the lowest was from Bubba Kush x OG Kush plant                (22.5 g). Cannabis cultivated wild-types yield of flowers varied from 104.7-39.9 g. According                to UPOV descriptors cultivated wild-type plants were distinguished from the commercial ones.                Analysis of cannabinoids in all cultivated wild-type plants exhibited a prevalence of CBD, with                total CBD concentrations ranging from 3.43% to 7.61% w/w. The highest CBD concentration                of  12.85%  w/w  was  determined  in  a  commercial  Charlotte’s Angel.  Principal  Component                Analysis (PCA) was performed with four main components that cumulatively explain 99% of                the  variability  of  the  parameters.  The  statistical  descriptors  of  the  Partial  Least  Square-                discriminatory  (PLS-DA)  model  (R2X=0.852,  R2Y=0.856,  Q2=0.817)  indicate  satisfactory                predictive capabilities for cannabinoid-based differentiation of the analyzed samples.                This study reveals the UPOV morphological distinctiveness of wild Macedonian Cannabis from                commercial  strains  and  underscores  its  prevalent  CBD  content,  suggesting  its  potential                registration as a distinct Macedonian Cannabis landrace.                                                                                                                  38","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL6                       New steroidal saponins from leaves of Agave bracteosa: structural                                       characterization and cytotoxic activity                                                                 2                                                                                    2                   Francesca Guzzo *, Alexandra García Durán , Laura López Rostoll , Francisco Antonio                                    1,                                                      2                                               Macías , Ana María Simonet                                                                           2                   1  Department of Biological and Pharmaceutical Environmental Sciences and Technologies-                  DiSTABiF, University of Campania \"Luigi Vanvitelli\", Via Vivaldi 43, 81100 Caserta, Italy.                  2  Allelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO);                              School of Science, University of Cádiz, Puerto Real, Cádiz, Spain.                                   *Corresponding author: francesca.guzzo@unicampania.it                 The genus Agave comprises more than 400 species and are known for their various uses and                applications, as source of fiber, food and beverages. Currently, species of this genus have been                investigated for their metabolic content, and, in particular, saponins are the compounds of main                interest. These  metabolites  play  a  critical  role  in  the  response  of  plants  to  different  stress                situations, and the use of different separation techniques is required, to achieve their structure                elucidation or the evaluation of their biological activity. Since no saponins have been described                for this species, the current work was addressed to the phytochemical study of Agave bracteosa                                                                                                   E,                leaves.  Dried  plant  material  was  extracted  and  screened  through  UPLC-QTOF-MS NMR                spectroscopy for the identification of the main saponins, and cytotoxicity assays. Subsequently,                the crude extract was chromatographed and the individual fractions so obtained purified by                HPLC, thus obtaining saponins in pure form. Characterization of each compound was carried                out  using  the  “HMAI  method”  (NMR  spectroscopy  and  the  HMBC  method  for  aglycone                identification),  for  aglycone  and  1D  and  2D  NMR  for  sugar  chains.  In  this  way,  steroidal                saponins were elucidated and tested for the cytotoxic activity.                 References:                1.     Sidana, J.; Singh, B.; Sharma, O.P. Saponins of Agave: chemistry and bioactivity. Phytochemistry 2016,                130, 22–46. https://doi.org/10.1016/j.phytochem.2016.06.010                2.     Oleszek, W.; Bialy, Z. Chromatographic determination of plant saponins—an update (2002–2005). J.                Chromatogr. A 2006, 1112, 78–91. https://doi.org/10.1016/j.chroma.2006.01.037                3.     Simonet, A.M.; Durán, A.G.; Pérez, A.J.; Macías, F.A. Features in the NMR spectra of the aglycones of                Agave spp. saponins. HMBC Method for Aglycone Identification (HMAI). Phytochem. Anal. 2021, 32, 38–61.                https://doi.org/10.1002/pca.2946                                                                                                                            39","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL7                      In situ characterisation of plant defence phytohormones and related                              metabolites via advanced mass spectrometry imaging                                              1                              1,                                                               2                                                                                   3                                                                                                   1,2                  Chao Zhang *, Jitka Siroka , Asta Žukauskaitė , Tetiana Kalachova , Karel Doležal , and                                                                    1                                                      Ondřej Novák                    1  Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of                      Sciences \& Faculty of Science, UPOL, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic                  2  Department of Chemical Biology, Faculty of Science, UPOL, Šlechtitelů 27, CZ-78371 Olomouc,                                                       Czech Republic                 3  Institute of Experimental Botany of the Academy of Sciences of the Czech Republic, Rozvojová 313,                                               165 02 Prague 6, Czech Republic                                          *Corresponding author: chao.zhang@upol.cz                 Phytohormones  are  a  group  of  small  signalling  molecules  that  play  an  important  role  in                activating  defense-related  gene  expressions  and  counteracting  exogenous  phytotoxic                metabolites.  Although  the  presence  of  defense  phytohormones  including  salicylic  acid,                jasmonic  acid,  ethylene  as  well  as  their  metabolites  have  been  identified  in  plant  immune                networks, the metabolomics interactions between host plants and invading pathogens are still                unclear. Identification of the large number of low molecular weight compounds resulting from                the plant defense response requires an untargeted metabolomic method with the implementation                of  multiple  analyte  detection  platforms.  Plant  biotic  stress  induced  by  pathogenic  microbe                infections  such  as  phytobacteria  or  oomycetes  represents  a  significant  problem  in  crop                           1                production .  However,  current  studies  focusing  on  host  plant  immunity  and  metabolites                involved  in  the  defense  response  are  very  limited.  We  have  recently  applied  label-free                desorption electrospray ionization mass spectrometry imaging (DESI-MSI) in high-throughput                visualizations of multiple phytohormone species and synthetic phytohormone derivatives from                Arabidopsis samples . DESI-MSI coupled with ion mobility was performed on the untargeted                                    2,3                analysis  of  plant  defense-related  compounds  from  Arabidopsis  leaves  pre-inoculated  with                Pseudomonas  syringae.  Our  results  demonstrated  unique  distributions  of  predicted  defense                phytohormones with their precursors and metabolites from infected and control leaves. Further                segmentation  analysis  of  infected  leaf  areas  established  different  spatial  correlations  of                endogenous molecules with selected microbial metabolites. This suggests that DESI-MSI is not                only able to interpret various biomolecules from both plant host and invasive microbes with up                to  200  μm  spatial  resolution,  but  also  provides  insight  into  plant  hormonomics  and                metabolomics changes under the microbially induced biotic stress.                 Acknowledgements: Authors  thank  to  Czech  Science  Foundation,  project  No.  22-17435S,                “Mass spectrometry approaches for plant immunity studies“.                References:                1.     Korniienko,  N.;  Kharina,  A.;  Budzanivska,  I.;  Burketová,  L.;  Kalachova,  T.  2022,  58,  81.                https://doi.org/10.17221/147/2021-PPS                2.     Zhang, C.; Žukauskaitė, A.; Petřík, I.; Pěnčík, A.; Hönig, M.; Grúz, J.; Široká, J.; Novák, O.; Doležal, K.                Analyst 2021, 146, 2653-2663. https://doi.org/10.1039/D0AN02118K                3.     Zhang, C.; Bieleszová, K.; Žukauskaitė, A.; Hladík, P.; Grúz, J.; Novák, O.; Doležal, K. Anal Bioanal                Chem 2024, 416, 125-139. https://doi.org/10.1007/s00216-023-04996-x                                                                                                              40","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL8                            Plant triterpenoid saponins – from structure to bioactivity                   Jan Günther*, Malbor Dervishi, Jincheng Shen, Rajeswari Gopal Geetha, Anja T. Fuglsang,                                               Pablo D. Cárdenas, Søren Bak                         Department of Plant and Environmental Sciences, University of Copenhagen,                                    Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark                                            *Corresponding author: jg@plen.ku.dk                 Saponins are triterpenoid amphipathic molecules that exhibit detergent-like properties. Due to                their potential ability to integrate into membranes with the hydrophobic triterpenoid moiety, it                is proposed that they wedge into membranes and thereby disrupt the cell membrane integrity of                herbivore pests, causing cell death. These specialized metabolites usually occur in its natural                source in a complex mixture of several diverse chemical structures and therefore it has been                difficult to determine individual saponin biological activities.                We present a fast and efficient way to fractionate and purify large amounts of single saponins                from a complex plant extract via flash chromatography coupled to UV and ELSD. Additionally,                we use yeast integrity assays to correlate saponin structure with biological activity. In this way,                we elucidate potentially highly bioactive saponins that bear potential for commercially useful                biopesticides. Furthermore, these purified saponins allow us to elucidate the general principle                of saponin mode of action.                Recently we have employed large unilamellar vesicles in order to test purified saponins and                study their behaviour in simple artificial membrane systems composed of different lipid and                sterol  compositions.  We  found  that  non-plant  derived  sterols  make  vesicles  susceptible  to                saponin induced pore formation whereas plant sterols protect vesicles from breaking.                 Acknowledgements: The authors thank the Novo Nordisk Foundation for the ECO-SAP Grant;                Grant No.: NNF20OC0060298, and Grant No.: NNF21OC0071296 for funding.                 References:ACS Style, maximum 3                                                    1–3                1.     Cárdenas, P. D.; Almeida, A.; Bak, S. Evolution of Structural Diversity of Triterpenoids. Front. Plant                Sci. 2019, 10 (2019), 1–10. https://doi.org/10.3389/fpls.2019.01523.                2.     Günther, J.; Erthmann, P. Ø.; Khakimov, B.; Bak, S. Reciprocal Mutations of Two Multifunctional b -                Amyrin Synthases from Barbarea Vulgaris Shift a / b -Amyrin Ratios. Plant Physiol. 2021, 1–13.                https://doi.org/10.1093/plphys/kiab545                3.     Liu, Q.; Khakimov, B.; Cárdenas, P. D.; Cozzi, F.; Olsen, C. E.; Jensen, K. R.; Hauser, T. P.; Bak, S. The                Cytochrome P450 CYP72A552 Is Key to Production of Hederagenin-Based Saponins That Mediate Plant                Defense against Herbivores. New Phytol. 2019, 222 (3), 1599–1609. https://doi.org/10.1111/nph.15689.                                                                                                                       41","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL9                   In vitro antidiabetic and anticholinesterase potentials of eleven medicinal                               plants from Lamiaceae naturally grown in Turkey                                                   1,                                      Hafize Yuca *, Ömer Çeçen , Songül Karakaya                                                                  2                                                                                    3                  1  Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey                   2  Department of Plant and Animal Production, Vocational School of Technical Sciences,                                   Karamanoğlu Mehmetbey University, Karaman, Turkey                 3  Department of Pharmaceutical Botany, Faculty of Pharmacy, Ataturk University, Erzurum,                                                          Turkey                                     *Corresponding author: hafize.yuca@atauni.edu.tr                 Lamiaceae family is one of the largest and most widespread flowering plant families, found                notably in the Mediterranean, Central and Minor Asia and is represented with over 7200 species                and  240  genera .  In  vitro  antidiabetic  and  anticholinesterase  activities  of  essential  oils,                                1                methanol,  and  aqueous  extracts  obtained  from  aerial  parts  of  Origanum  vulgare,  O.                leptocladum,  Nepeta  sulfuriflora,  N.  isaurica,  N.  cilicica,  Thymus  cilicicus,  Ziziphora                clinopodioides,  Cyclotrichium  origanifolium,  Hyssopus  officinalis,  Ajuga  chamaepitys,                Marrubium globosum growing in Turkey were herein examined. The antidiabetic activity was                evaluated  against  both  α-glucosidase  and  α-amylase.  Anticholinesterase  activity  was  tested                against both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) . Among the tested                                                                                         2                essential  oils,  O.  leptocladum  and  N.  isaurica  exhibited  the  highest  activity  against  α-                glucosidase, with inhibitions of 14.39 % and 14.25 %, respectively, while acarbose showed                74.72% inhibition. In terms of extracts, both MeOH and aqueous extracts of O. leptocladum                displayed significant inhibitions, with 90.10% and 76.22% inhibition, respectively. T. cilicicus                MeOH extract also showed notable inhibition at 60.05%, surpassing the effect of acarbose. For                α-amylase  inhibition,  C.  origanifolium  and  T.  cilicicus  essential  oils  exhibited  the  highest                activities, with inhibitions of 49.54% and 42.67%, respectively, while acarbose showed 67.87%                inhibition. Among the extracts,  M. globosum and  N. cilicica MeOH extracts displayed the                highest  inhibitions,  with  rates  of  33.76%  and  31.66%,  respectively.  In  anticholinesterase                inhibition assays, C. origanifolium essential oil showed the highest activity against AChE with                11.84% inhibition, and O. leptocladum displayed 17.06% inhibition against BChE. Among the                extracts, O. vulgare MeOH extract exhibited the best activity against AChE at 29.10%, while                T. cilicicus showed the highest activity against BChE at 35.44%. Donepezil demonstrated high                efficacy in both experiments, from both essential oils and extracts.                 References:                1.     Kanjevac, M., Zlatić, N., Bojović, B., \&  Stanković, M. Pharmaceutical and biological properties of                Stachys species: A review. Braz. J. Pharm. Sci. 2023, 58. https://doi.org/10.1590/s2175-97902022e20211                2.     Karakaya, S., Yuca, H., Yılmaz, G., Aydın, B., Tekman, E., Ekşi, G., Bona, M., Göger, G., Karadayı, M.,                Gülşahin, Y., Öztürk, G., Demirci, B., \& Güvenalp, Z. Phytochemical screening, biological evaluation, anatomical,                and  morphological  investigation  of  Ferula  tingitana  L.  (Apiaceae).  Protoplasma  2023,  260,  1581–1601.                https://doi.org/10.1007/s00709-023-01874-2                                                                                                                42","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL10                  Cellular responses and targets in food-spoilage yeasts exposed to antifungal                                               prenylated isoflavonoids                                                    3                                                                                      4                                                                 3                                                                                                       4                   Sylvia Kalli 1,2, *, Cindy Vallieres , Joseph Violet , Jan-Willem Sanders , John Chapman ,                                                                                           1                                                 1                                                                  3                               Jean-Paul Vincken , Simon V. Avery  and Carla Araya-Cloutier                   1  Laboratory of Food Chemistry, Wageningen University \& Research, P.O. Box 17, 6700 AA                                               Wageningen, the Netherlands                  2  Wageningen Food Safety Research, Part of Wageningen University \& Research, European                      Union Reference Laboratory plant toxins and mycotoxins, P.O. Box 230, 6700 AE,                                               Wageningen, the Netherlands                      3  School of Life Sciences, University of Nottingham, Nottingham, United Kingdom                  4  Unilever Foods Innovation Centre, Bronland 14, 6708 WH Wageningen, the Netherlands                                         *Corresponding author: sylvia.kalli@wur.nl                 Prenylated isoflavonoids are phytochemicals with promising antifungal properties. The highly                promising, antifungal prenylated isoflavonoids, glabridin and wighteone were shown to disrupt                the plasma membrane (PM) of the food spoilage yeast Zygosaccharomyces parabailii in distinct                ways. This observation prompted a more in-depth investigation into their modes of antifungal                action (MoA) by using advanced molecular tools on spoilage yeasts. Transcriptomic profiling                with Z. parabailii showed that genes encoding transmembrane ATPase transporters, including                Yor1  and  genes  homologous  to  the  PDR  subfamily  in  Saccharomyces  cerevisiae,  were                upregulated in response to both compounds. Gene functions involved in fatty acid and lipid                metabolism, proteostasis and DNA replication processes were over-represented among genes                upregulated by glabridin and/or wighteone. Chemogenomic analysis using the genome-wide                deletant collection for S. cerevisiae, further suggested an important role for PM lipids and PM                proteins for both compounds. Using lipid biosynthesis inhibitors, we corroborated roles for                sphingolipids and ergosterol in prenylated isoflavonoid action. The PM ABC transporter Yor1,                and  Lem3-dependent  flippases  conferred  sensitivity  and  resistance,  respectively,  to  the                compounds,  suggesting  an  important  role  for  PM  phospholipid  asymmetry  in  their  MoAs.                Impaired tryptophan availability, likely linked to perturbation of the PM tryptophan permease                Tat2, was evident in response to glabridin. Last, substantial evidence highlighted a role of the                endoplasmic  reticulum  (ER)  in  cellular  responses  to  wighteone,  including  gene  functions                associated with ER membrane stress or with phospholipid biosynthesis, the primary lipid of the                ER  membrane.  Overall,  this  study  highlights  that  cellular  actions  of  these  two  prenylated                isoflavonoids share similarities (at the level of the plasma membrane) but also differences.                Understanding  the  mode  of  action  of  these  novel  antifungal  agents  is  essential  for  their                application in food preservation. This study exemplifies the use of genomic tools combined                with testing of arising hypotheses to shed new light on the antifungal action of prenylated                isoflavonoids.                              1                 1.     Kalli, S.; Vallieres, C.; Violet, J.; Sanders, J.-W.; Chapman, J.; Vincken, J.-P.; Avery, S. V.; Araya-Cloutier,                C.  Cellular Responses  and Targets  in  Food  Spoilage Yeasts Exposed  to Antifungal  Prenylated  Isoflavonoids.                Microbiology Spectrum 2023, 11 (4), e01327-01323. https://doi.org/ 10.1128/spectrum.01327-23                                                                                                               43","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL11                  Integration of plant-based antimicrobials and magnetic fields for enhanced                                          penetration of pathogenic biofilms                                                                                            2                                                    1                                1,                                                                        1                    Adam Junka *, Malwina Brożyna , Bartłomiej Dudek , Karol Fijałkowski , Magdalena                                                                     1                                              1                                  Markowska , Jędrzej Dzięgielewski , Wojciech Rybicki                                                                                        1                  1  Platform for Unique Model Application, Department of Pharmaceutical Microbiology and                     Parasitology, Wrocław Medical University, Borowska 211, 50-534 Wrocław, Poland                   2  Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal                   Husbandry, West Pomeranian University of Technology in Szczecin, Piastow 45, 70-311                                                     Szczecin, Poland                                      *Corresponding author: adam.junka@umw.edu.pl                 The  escalating  challenge  of  antibiotic  resistance  underscores  the  urgent  need  for  novel                                     1                therapeutic strategies . Plant-derived antimicrobials, with their broad and non-specific modes                of action, exemplified by compounds such as 1,8-cineole, thymol, and abietic acid, offer a                promising avenue against pathogenic biofilms. However, the protective extracellular matrix of                biofilms significantly impedes the penetration of these compounds, limiting their efficacy . In                                                                                                       2                this study, we introduce a novel approach employing a magnetic field with precisely controlled                parameters, including frequency and waveform, to disrupt the integrity of the biofilm matrix                and alter microbial cell walls. This innovation enables plant-derived antimicrobials to infiltrate                biofilms  more  effectively  and  exert  their  antimicrobial  action.  Our  findings  suggest  that                integrating magnetic field exposure with plant-based antimicrobials can significantly reduce                the  required  effective  concentration  of  these  compounds  by  up  to  several  dozen  percent,                depending on the specific antimicrobial, microbial strain, and magnetic field characteristics.                This reduction has promising implications for the pharmacokinetics and pharmacodynamics of                antimicrobials designed to combat biofilms, particularly those associated with chronic wounds,                                                          3                pulmonary  infections,  and  osteomyelitis .  Our  research  opens  new  pathways  for  the                development of more efficient, targeted treatments for biofilm-related infections, highlighting                the potential of combining plant-based antimicrobials with magnetic field technology.                 Acknowledgements: Authors thank to Agency National Science Centre, Poland, for providing                resources within grant number: 2021/41/N/NZ6/03305                 References:                1.     Grooters  KE.  Strategies  for  combating  antibiotic  resistance  in  bacterial  biofilms.  Front  Cell  Infect                Microbiol. 2024, 19; 14:1352273. doi:10.3389/fcimb.2024.1352273.                2.     Goodman SD. Bacterial Biofilms Utilize an Underlying Extracellular DNA Matrix Structure That Can Be                Targeted for Biofilm Resolution. Microorganisms. 2022, 18;10. doi:10.3390/microorganisms10020466.                3.     Thaarup  IC.  Biofilm  Survival  Strategies  in  Chronic  Wounds.  Microorganisms,  2022;  10(4)                doi:10.3390/microorganisms10040775                                                                                                                  44","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL12                  Evaluation of the chemical content and biological activities of “Orobanche                                                        ramosa” L.                                                                                                       2                                                        1                               1,                    Selen İlgün *, Sena Akçakaya Mutlu , Esra Köngül Şafak , Gökçe Şeker Karatoprak ,                                                                            2                                                                    3                                                      Gülsüm Yıldız                   1  Department of Pharmaceutical Botany, Faculty of Pharmacy, Erciyes University, Kayseri,                                                          Türkiye                  2  Department of Pharmacognosy, Faculty of Pharmacy, ErciyesUniversity, Kayseri, Türkiye                   3  Department of Pharmacognosy, Faculty of Pharmacy, Van Yuzuncu Yil University, Van,                                                          Türkiye                                       *Corresponding author: erturkselen@gmail.com                 The  Orobanche  genus,  belonging  to  the  Orobanchaceae  family,  is  globally  distributed  and                entirely parasitic in nature. Orobanche species have been found to contain a diverse array of                compounds, including phenylethanoid glycosides, steroids, terpenoids, organic acids, alkaloids,                lignans,  and  flavonoids.  These  plants  are  utilized  in  traditional  medicinal  practices  across                Europe, America,  and Africa.  Extracts  of  the  plant,  collected  from  agricultural  lands  and                identified,  underwent  comprehensive  LC-MS/MS  analysis,  quantifying  53  phytochemicals.                Antioxidant (DPPH and ABTS assay), enzyme inhibition (anti-tyrosinase, anti-elastase, anti-                acetylcholinesterase, and α-glucosidase), and cytotoxic activities were evaluated. The highest                total phenol and flavonoid content were observed in the butanol extract at 333.32±5.3 mg GA/g                extract  and  298.31±7.7  mg  RE/gextract,  respectively.  LC-MS/MS  analysis  revealed  quinic  acid                (58.619 mg/gextract) in methanol extract, protocatechuic acid (3.478 mg/gextract) in ethyl acetate                extract, and vanillic acid (6.198 mg/gextract) in butanol extract among the compounds detected                in high amounts. Evaluation of the biological activities of the extracts demonstrated significant                ABTS and DPPH radical scavenging effects for ethyl acetate, butanol, and methanol extracts.                In cancer cell lines, cell viability was 18% for HeLa-CCL-2 cells treated with ethyl acetate                extract at 1000 µg/mL, and 15.51% and 13.86% for COLO 205-CCL-222 cells treated with                hexane and ethyl acetate extracts, respectively, at the same concentration. Butanol, hexane, and                ethyl acetate extracts exhibited significant tyrosinase inhibitory activity of 99%, 97%, and 91%,                respectively,  at  a  concentration  of  2500  µg/mL. Additionally,  hexane  extract  demonstrated                strong α-glucosidase enzyme inhibition activity (95% at 1 mg/mL concentration).                 Acknowledgments:  Authors  thanks  to  Erciyes  University  Scientific  Research  Projects                Coordination Office, for TSA-2023-13000 “Evaluation of the Chemical Content and Biological                Activities of “Orobanche ramosa” L.”                  References:                1.     Shi,  Ruyu,  et  al. The  genus  Orobanche  as  food  and  medicine: An  ethnopharmacological  review.  J.                Ethnopharmacol. 263, 2020: 113154. https://doi.org/10.1016/j.jep.2020.113154                                                                                                                45","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL13                 The interaction of silymarin with the gut microbiota and its differences with                                                             age                                                                                                 1                                                                       1                                                          1                                     1,                   Katerina Tomisova *, Veronika Jarosova , Petr Marsik , Anna Mascellani Bergo , Ondrej                                                                              3                                            2                                                                                                  4                    Cinek , Lucie Hlinakova , Pavel Kloucek , Vaclav Janousek , Katerina Valentova , and                         2                                                            1                                                      Jaroslav Havlik 1                 1  Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University                          of Life Sciences Prague, Kamycka 129, 165 00 Prague, Suchdol, Czech Republic                   2  Department of Pediatrics, 2  Faculty of Medicine, Charles University and University Hospital                                             nd                                       Motol, V Uvalu 84, 150 06 Prague, Czech Republic                                  3  Biodviser Ltd, 2 Pass St, OL9 6HZ Oldham, United Kingdom                 4  Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 00 Prague, Czech                                                          Republic                                         *Corresponding author: tomisova@af.czu.cz                 This study evaluates the interaction between dietary silymarin, a flavonolignan complex from                milk  thistle, and the gut  microbiota, highlighting the effect of age on this interaction. The                investigation used a combination of next-generation sequencing (NGS),  H-NMR, and LC-MS                                                                                      1                technologies to study the  interactions between silymarin and the gut microbiota  in healthy                young (12–45 years) and healthy elderly (70–80 years) individuals whose microbiota differ.                Twenty microbial silymarin catabolites were identified. The biosynthesis of short-chain fatty                acids was found to be suppressed by silymarin. The study also revealed significant age-related                differences in silymarin metabolism, with older subjects producing two silymarin metabolites                at a significantly higher rate than younger subjects. It was found that the production of these                catabolites,  which  are  typical  of  older  donors,  was  related  to  the  abundance  of  the  genus                Oscillibacter. In contrast, the genus Faecalibacterium was more abundant in the younger group.                This was correlated with the degradation of silymarin components. Additionally, the presence                of silymarin altered the properties of the intestinal microbiota by increasing its alpha diversity                at the order level. These findings highlight the importance of age-specific dietary strategies and                contribute to our understanding of the role of the gut microbiome in flavonolignan metabolism.                The  insights  gained  from  this  study  provide  a  basis  for  the  development  of  personalized                nutritional interventions to improve gut health at different stages of life.                 Acknowledgements:  Authors  thank  to  Grant  Agency  of  the  Czech  Republic  (GACR  23-                04655S),  the  Faculty  of Agrobiology,  Food  and  Natural  Resources  (FAFNR)  at  the  Czech                University  of  Life  Sciences  Prague  (SV22-5-21310),  a  METROFOOD-CZ  research                infrastructure project (MEYS Grants LM2023064 and LM2018100), including access to its                facilities, and a grant from the Ministry of Education, Youth and Sports of the Czech Republic                (Talking  Microbes  –  Understanding  Microbial  Interactions  within  One  Health  Framework;                CZ.02.01.01/00/22_008/0004597).                                                                                                                46","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL14                    Characterization and sustainable agrochemical application of Vessalico                                                     garlic ecotype                               1,                                                                     3                                                                                     4                                                                                                        4                                                2                 Valeria Iobbi *, Valentina Parisi , Anna Paola Lanteri , Norbert Maggi , Mauro Giacomini ,                                                                                       3                                                    1                                                                       3                    Giuliana Drava , Marta Lo Vetere , Giovanni Minuto , Andrea Minuto , Nunziatina De                                   1                                                          2                                                 Tommasi , Angela Bisio                                                                         1                   1  Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy                     2  Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084                                                       Fisciano, Italy                    3  Centro di Sperimentazione e Assistenza Agricola (CeRSAA), Regione Rollo 98, 17031                                                       Albenga, Italy                   4  Department of Informatics, Bioengineering, Robotics and System Science, University of                                      Genova, Via Opera Pia 13, 16145 Genova, Italy                                      *Corresponding author: valeria.iobbi@edu.unige.it                 Vessalico  garlic  (“Aglio  di  Vessalico”),  one  of  the  best-known  Italian  garlic  ecoytpes,  is                cultivated in Ligurian region, being very appreciated for its shape and aroma, and considered                as agri-food excellence of the region. Local farmers use every year garlic cloves of two border                French cultivars, Messidor and Messidrôme for sowing, and this fact can affect the “Protected                Designation of Origin” registration process. To improve the geographical characterization of                Vessalico garlic and to identify its peculiar features in terms of bioactive compounds, LC/MS                followed by multivariate data analysis, was firstly applied. Results allowed to differentiate the                Vessalico garlic from the French cultivars, and to identify three sub-classes in the accessions of                Vessalico  ecotype. An  NMR-based  analytical  approach  (1,  2)  was  then  applied  to  identify                characteristic  chemical  markers  to  classify  the  product  of  the  farms  of  the  Vessalico                geographical  area  as  true  Vessalico  garlic.  Results  showed  that  the  content  of  primary                metabolites  appeared  to  be  more  related  to  genetic  characteristics,  while  the  secondary                metabolites appeared to be  more related to the area and  to the methods of cultivation and                harvesting.  Self-organizing  maps  (SOMs)  showed  that  only  one  homogeneous  product  of                Vessalico  appeared  well  characterized  compared  to  the  others.  Among  the  secondary                metabolites,  S-allylcysteine was quantified, leading to the possibility of considering it  as a                marker  to identify Vessalico garlic. Finally,  to promote  a sustainable agriculture, Vessalico                garlic  extracts  were  investigated  as  possible  agrochemicals. All  extracts  showed  ability  to                deactivate two plant virus infectivity, as a possible consequence of disassembly of the virus coat                protein (CP). Molecular docking showed a strong interaction of the sulfur compounds with a                high number of residues into ToBRFV CP binding site.                 References:                1.     Chenomx software (Chenomx NMR Suite 8.6, Chenomx Inc., Edmonton, 252 Canada).                2.     Jacob, D. NMRProcFlow: a graphical and interactive tool dedicated to 1D spectra processing for NMR-                based metabolomics. Metabolomics. 2017; 13: 36. https://doi.org/10.1007/s11306-017-1178-y                                                                                                               47","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL15                     Anti-Alzheimer effects of Paradol, mansorin A, and mansonone G in a                       zebrafish model of Alzheimer’s disease induced with okadaic acid                                              Iasmina Honceriu *, Lucian Hritcu 1                                                             1,                       1  “Alexandru Ioan Cuza” University of Iasi, 20A Carol I Boulevard, Iasi, Romania                                  *Corresponding author: iasmina.honceriu@student.uaic.ro                 Alzheimer's Disease (AD) is the most prevalent type of dementia and is still incurable. Given                its  high  socio-economic  impact,  the  World  Health  Organization  stated  that  novel  anti-AD                treatments  are  imperative  to  discover  and  develop.  Paradol  (PD),  mansorin  A  (MA),  and                mansonone G (MG) are plant-derived phenolic ketones with neuroprotective effects. This study                explored the anti-AD effects of PD, MA, and MG using an okadaic acid-induced zebrafish                model of AD. First, the animal model was induced by immersion of animals in okadaic acid 10                nM for four days, then treated with PD, MA, and MG, each in three concentrations: 1, 3, and 6                μg/L. The study used several tests to evaluate the effects of these treatments on the animal                model. Y-maze and Novel Object Recognition (NOR) tests were performed to assess short-term                memory in the experimental groups. The Novel Tank Diving Test (NTT) and Novel Object                Approach  Test  (NOA)  were  used  to  evaluate  the  anxiety-like  state.  Biochemical  analysis                assessed the levels of oxidative stress markers and the levels of acetylcholinesterase. In silico                analysis was performed to investigate some physicochemical and pharmacokinetic parameters.                The results indicate the promnesic effects of PD, MA, and MG by enhancing the short-term                spatial  memory  in  the  animal  model,  as  indicated  by  the Y-maze  task  and  the  short-term                recognition memory (NOR test). They also have anxiolytic benefits, according to the results of                the NTT and NOA tests. PD, MA, and MG act as antioxidants in the animal model by increasing                the  catalase  and  reduced  glutathione  levels.  They  also  inhibited  the  acetylcholinesterase                activity. The in silico analysis indicated that PD, MA, and MG are blood-brain barrier permeant                drug-like  molecules.  In  conclusion,  through  promnesic,  anxiolytic,  antioxidant,  and                acetylcholinesterase inhibitory properties, MA, MG, and PD are potential dementia treatments                with good bioavailability.                                                                                                                                  48","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL16                    The antiproliferative activity and apoptosis-inducing mechanism of the                   Amaryllidaceae alkaloid montanine on A549 and MOLT-4 human cancer                                                           cells                                                                1                                 1                                               2                                                                                3                                                                                                  2                  Darja Koutova , Negar Maafi , Darina Muthna , Karel Kralovec , Jana Kroustkova , Filip                                                1                          2                                                                                                      1                                                                                   2                                                                 4                   Pidany , Abdul Aziz Timbilla , Eva Cermakova , Lucie Cahlikova , Martina Rezacova ,                                                                    1,                                                     Radim Havelek *                   1  Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles                              University, Simkova 870, Hradec Kralove 500 03, Czech Republic                  2  ADINACO Research Group, Department of Pharmacognosy and Pharmaceutical Botany,                 Faculty of Pharmacy, Charles University, Heyrovskeho 1203, Hradec Kralove 500 05, Czech                                                         Republic                   3  Department of Biological and Biochemical Sciences, Faculty of Chemical Technology,                         University of Pardubice, Studentska 573, Pardubice 532 10, Czech Republic                    4  Department of Medical Biophysics, Faculty of Medicine in Hradec Kralove, Charles                              University, Simkova 870, Hradec Kralove 500 03, Czech Republic                                        *Corresponding author: havelekr@lfhk.cuni.cz                  There  is  increasing  interest  in  isoquinoline  alkaloids  from Amaryllidaceae  plants  for  new                anticancer drugs. Montanine-type alkaloids are a less common subclass in this family, yet their                specific  anticancer  mechanisms  remain  poorly  documented.  In  this  study,  we  screened                montanine, manthine, and 15 semisynthetic montanine analogues at a test dose of 10 μM to                explore  their  cytotoxic  activities.  In  the  initial  single-dose  screening,  montanine  and  its                derivatives, specifically 12 and 14, exhibited the highest cytostatic activity. However, native                montanine demonstrated the most potent antiproliferative activity. Montanine also displayed                significant antiproliferative activity with IC50 values of 1.09 µM and 1.26 µM against A549 and                MOLT-4 cells, respectively. Investigations on cell death mechanisms revealed ability to induce                apoptosis  in  MOLT-4  cells  through  caspase  activation,  mitochondrial  depolarization,  and                Annexin V staining. The Western blot demonstrated that montanine induced the upregulation                or  activation  of  pro-apoptotic  and  cell  cycle  checkpoint  proteins,  including  p21,  Bax,  p38                MAPK phosphorylated at Thr180/Tyr182, and Chk1 phosphorylated at Ser345. These findings                provide novel  insights into the  cytostatic,  cytotoxic, and proapoptotic  effects of montanine                alkaloid in lung adenocarcinoma A549 and acute lymphoblastic leukemia MOLT-4 cells.                 Acknowledgements: This work was supported by the Cooperatio Program, research area DIAG                and SVV-2023-260656 of the Charles University.                 References:                Koutova  D,  Maafi  N,  Muthna  D,  et  al.  Antiproliferative  activity  and  apoptosis-inducing  mechanism  of                Amaryllidaceae alkaloid montanine on A549 and MOLT-4 human cancer cells. Biomed Pharmacother. 2023, 166,                115295. doi:10.1016/j.biopha.2023.115295                                                                                                                49","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL17                       Analgesic and anti-inflammatory activity of some Glaucium taxons                                                                     1,                                                    Melek Karaaslan *                    1  Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, Yenimahalle,                                                          Ankara                                     *Corresponding author: mkaracaoglu@ankara.edu.tr                 The genus Glaucium Mill. (Papaveraceae) is distributed in Europe, Southeast and Central Asia,                North Africa and North America with approximately 30 species, and is represented by 11 taxa                in Turkey. Plants of this genus stand out especially with the isoquinoline alkaloids they contain.                In  the  content  of  this  study,  the  analgesic  and  anti-inflammatory  effects  of  Glaucium                corniculatum (L.) Rud. subsp. refractum (NAB) Cullen ve Glaucium leiocarpum Boiss. were                evaluated. The ethanolic extracts and alkaloid fractions were prepared using the aerial parts.                LC-MS analyses were conducted for the purpose of evaluation of the phytochemical profiles of                the total extracts and the alkaloid fractions.                Carrageenan-induced hind paw edema test was conducted on Wistar Albino rats in order to                determine the anti-inflammatory activity, while tail flick and hot plate tests were performed on                Mus musculus Balb/C mice for the evaluation of analgesic activity. For all three experimental                models, the animals were divided into twelve groups comprising 6 members in each, namely;                control, reference, extract groups (for the doses of 50; 100; 200 mg/kg) and fraction groups (for                the doses of 50; 100 mg/kg).                The ethanolic extract of G. leiocarpum (200 mg/kag) was found to possess anti-inflammatory                activity between the 2nd and 6th hours, while the alkaloid fraction of G. corniculatum (100 and                50 m/kg) inhibited the edema between 1st and 3rd hours, although the activity was not found                to be in a dose-dependent manner. As the result of analgesic activity tests, it was determined                that the ethanolic extract of G. leiocarpum showed the highest activity at the dose of 200 mg/kg.                The results of the study revealed that the total extract of  G. leiocarpum exhibited a higher                analgesic and anti-inflammatory effects than the alkaloid fractions.                 Acknowledgements:  This  study  was  supported  by Ankara  University,  Scientific  Research                Projects Coordination Unit (BAP) (Project Code: 20B0237005).                                                                                                                               50","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL18                      Valorisation of North Atlantic macroalgae: Antimicrobial activity of                                                     phlorotannins                                                              1                                                                         2                           Adrian H. Kopf *, Anna Hammer , René Dirks , Carla Araya-Cloutier   1                                          1,                  1  Laboratory of Food Chemistry, Wageningen University and Research, Bornse Weilanden 9,                                          6708WG, Wageningen, The Netherlands                 2  Wageningen Food Safety Research, Wageningen University and Research, Akkermaalsbos                                         2, 6708WB, Wageningen, The Netherlands                                         *Corresponding author: adrian.kopf@wur.nl                 Multidrug-resistant bacteria are a severe threat to society. Discovery of novel antimicrobials                with distinctive mechanisms of action is pertinent. Marine environments, specifically, seaweeds                (macroalgae) are excellent sources to look for new bioactive compounds. Due to the harsh                environment that seaweeds grow in, they produce a variety of bioactives with unique chemical                         1                scaffolds .                In this work, fourteen species of seaweed harvested in the North Atlantic (North Sea) waters                were screened for their antimicrobial potential. Crude extracts were obtained by ultrasound-                assisted extraction using sequential solvents of varying polarity. Their antimicrobial activity                was  assessed  in  a  particularly  sensitive  agar-well  diffusion  assay  against  bacteria  Bacillus                                                                                                     2                cereus, B. pumilus, B. subtilis, Micrococcus luteus, and Yersinia ruckeri (NAT-screen) . At a                concentration of 1 mg/mL, 57 out of the 85 extracts showed antimicrobial activity, with the                largest  zones  of  inhibition  (~7  mm)  detected  in  both  highly  apolar  (hexane)  and  polar                (methanol) solvent extracts. The most potent antimicrobial activity was observed for brown                seaweed  Ascophyllum  nodosum,  Fucus  vesiculosus,  Sargassum  pallidum,  and  Undaria                pinnatifida.                As a highlight, methanol extracts (A. nodosum and F. vesiculosus) were further investigated by                activity-guided fractionation through purification with FLASH chromatography and analysis                by RP-UHPLC-PDA-ESI-MS. Bioactive fractions were found to be enriched in phlorotannins                                                                            3                (polymers of phloroglucinol units, such as fucophlorethol A) . This class of unique phenolic                compounds shows promise for the valorisation of brown seaweed species.                This  work  demonstrates  the  systematic  study  of  seaweeds,  including  the  isolation  and                characterization of bioactive secondary metabolites. In summary, we have developed a highly                sensitive pipeline for non-targeted screening and characterization of antimicrobial compounds                in seaweeds, for the valorisation of this abundant marine resource.                 References                1.  Pérez, M. J.; Falqué, E.; Domínguez, H. Antimicrobial Action of Compounds from Marine Seaweed. Marine                    Drugs, 2016, 14(3), 52. https://doi.org/10.3390/md14030052                2.  Pikkemaat, M. G.; Oostra-van Dijk, S.; Schouten, J.; Rapallini, M.; van Egmond, H. J.  A new microbial                    screening method for the detection of antimicrobial residues in slaughter animals: The Nouws antibiotic test                    (NAT-screening). Food Control, 2008, 19, 8, 781-789. https://doi.org/10.1016/j.foodcont.2007.08.002                3.  Vissers, A. M.; Caligiani, A.; Sforza, S.; Vincken, J.-P.; Gruppen, H. Phlorotannin Composition of Laminaria                    digitata. Phytochem. Anal. 2017, 28(6), 487-495. https://doi.org/10.1002/pca.2697                                                                                                               51","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL19                  Spice-derived bioactive components inhibit cancer metastasis by targeting                                             cellular signaling pathways                      Shamee Bhattacharjee*, Ashikur Rahaman, Arnab Sarkar, Jahnabi Ghosal, Deba Prasad                                                          Mandal                             Department of Zoology, West Bengal State University, Kolkata, India                                     *Corresponding author: shamee.zoology@wbsu.ac.in                 Spices have been used to impart flavour, colour and taste to cuisines in various countries. The                distinct aroma of spices, which provide an ethnic identity to cuisines, is contributed by their                constituent bioactive secondary metabolites. Owing to these phytochemicals, the culinary use                of  spices  is  complemented  by  several  health-promoting  benefits  including  anti-cancer                potential . Cancer is a significant public health challenge globally with metastasis causing 90%                         1                of  all  cancer-related  deaths. Therefore,  development  of  anti-metastatic  agents  is  extremely                         2                pertinent .  We  present  here  the  in  vitro  and  in  vivo  anti-metastatic  activity  of  two  spice                principles, 1,8-cineole (active component of cardamom) and capsaicin (active component of                chili peppers) in cancer cells and murine transplantable tumor models. Scratch wound assay,                transwell  migration  assay,  gelatine  zymography  and  detection  of  molecular  markers  of                metastasis and epithelial to mesenchymal transition (EMT) by qPCR, immunofluorescence and                immunoblotting were performed.                1,8-cineole and capsaicin respectively inhibited B16 melanoma and AGS gastric carcinoma cell                migration and invasion accompanied by a reduction in mesenchymal markers such as Slug,                Snail and Vimentin and increase in the epithelial marker E-cadherin. The phytochemicals also                downregulated matrix metallo protease (MMP) activities. The results were validated in vivo                using transplantable tumor models viz. B16 melanoma and 4T1 breast carcinoma. Finally, we                also  demonstrated  that  the  anti-metastatic  activity  of  1,8  cineole  and  that  of  capsaicin  is                mediated by targeting the PI3K/Akt/mTOR and TGFβ signalling pathways respectively.                Collectively the results underline the potential of these phytochemicalsas anti-metastatic agents.                However, the application of spice active principles in the treatment of cancer is often marked                                                                          3                with caution because of their hormetic effect on cancer cells . Therefore, future studies should                be directed at assessing the effect of phytochemicals over a wide-range of concentrations alone                and in combination with conventional chemotherapeutic agents.                 Acknowledgements: Authors thank ICMR, Grant No.5/13/49/2010/NCD-III,“An Investigation into                the regulation of Nrf2 transcription factor by diosgenin and 1, 8-cineole using the p53/p21 axis in lung                and  leukaemic  cancer”\&  DBT  Grant  No.  BT/PR15116/GBD/27/327/2011,  “Investigation  into  the                modulation of TGFb signaling by capsaicin and eugenol using the p53/p21 axis in gastric carcinoma.”                 References:                1.     Talib WH, AlHur MJ, Al Naimat S, Ahmad RE, Al-Yasari AH, Al-Dalaeen A, Thiab S, Mahmod AI.                Anticancer Effect of Spices Used in Mediterranean Diet: Preventive and Therapeutic Potentials. Front Nutr. 2022,                14;9: 905658. doi: 10.3389/fnut.2022.905658                                                                                                              52","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL20                    Antibacterial prenylated (iso)flavonoids from Fabaceae: inhibition and                        permeabilization of methicillin-resistant Staphylococcus aureus                                                        2                                                                         1                                   1,2,                                                                                            3                    Janniek Ritsema *, Nynke Kramer , Wouter de Bruijn , Maurice Franssen , Jean-Paul                                                      1                                              Vincken , Carla Araya-Cloutier 1                   1  Laboratory of Food Chemistry, Wageningen University \& Research, Bornse Weilanden 9,                                          6709 WG Wageningen, The Netherlands                    2  Toxicology Chair group, Wageningen University \& Research, Stippeneng 4, 6708 WE                                               Wageningen, The Netherlands                 3  Laboratory of Organic Chemistry, Wageningen University \& Research, Stippeneng 4, 6708                                             WE Wageningen, The Netherlands                                       *Corresponding author: janniek.ritsema@wur.nl                 Prenylated  (iso)flavonoids  are  plant-derived  compounds  with  potent  antimicrobial  activity                against  Methicillin-resistant  Staphylococcus  aureus  (MRSA).  They  primarily  act  on  the                bacterial  membrane  and  disrupt  membrane  integrity  by  fast  permeabilization . Very  potent                                                                                             1                antimicrobial diprenylated (iso)flavonoids, however, did not show membrane permeabilization                                         1                in Listeria monocytogenes . So far, the permeabilization capacity of prenylated (iso)flavonoids                is only assessed at singular concentrations, making it impossible to establish dose-response                relationships and accurately compare permeabilization potencies. In this study antimicrobial                activity and permeabilization capacity of 36 different prenylated (iso)flavonoids were assessed                against MRSA. Antimicrobial activity was determined by the broth microdilution assay and                membrane permeabilization by measuring the uptake of propidium iodide using fluorescence                microscopy  and  spectroscopy.  Dose-response  curves  were  established  and  used  to  extract                effective concentrations (EC10).                No  relationship  between  antimicrobial  activity  and  permeabilizing  capacity  was  found  for                compounds having good antimicrobial activity (minimum inhibitory concentration (MIC) ≤ 50                μg/mL).  Luteone  (1)  (EC10:  23±7  μg/mL)  and  neobavaisoflavone  (2)  (EC10:  28±8  μg/mL)                showed most potent permeabilization but were not the most potent antimicrobials (MIC of  25                and 38 μg/mL, respectively). Molecular descriptors analyses revealed that permeabilization                (EC10)  could  not  be  explained  by  relative  hydrophobic  surface  area,  as  found  for  Listeria                monocytogenes. Conversely, polar surface area was positively correlated to pEC10, suggesting                that  membrane  permeabilization  occurs  through  interactions  with  polar  phospholipid                headgroups. These results highlight the complexity of prenylated (iso)flavonoids’ antimicrobial                action, suggesting other (membrane) effects besides permeabilization.                 References:                1.     Araya-Cloutier, C.; Vincken, J.-P.; van de Schans, M. G. M.; Hageman, J.; Schaftenaar, G.; den Besten,                H. M. W.; Gruppen, H. QSAR-based molecular signatures of prenylated (iso)flavonoids underlying antimicrobial                potency against and membrane-disruption in Gram positive and Gram negative bacteria. Scientific Reports 2018,                8 (1), 9267. DOI: 10.1038/s41598-018-27545-4                                                                                                              53","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL21                        Carltonines from Narcissus pseudonarcissus cv. Carlton and lead                                                      optimization                            Jana Křoustková *, Filip Pidaný , Abdullah Al Mamun , Lucie Cahlíková  1                                                         1                                                                               1                                          1,                  1  Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles                           University, Heyrovského 1203, 500 03 Hradec Králové, Czech Republic                                        *Corresponding author: marikoj2@faf.cuni.cz                 The  newly  discovered  alkaloids,  carltonines  A-E,  were  isolated  from  Narcissus                pseudonarcissus  cv.  Carlton.  Following  comprehensive  structure  analysis,  the  pure  new                compounds  were  evaluated  for  in  vitro  inhibitory  activity  on  human  acetylcholinesterase                (hAChE), butyrylcholinesterase (hBChE), and propyl oligopeptidase. Notably, they exhibited a                                                                                   1                selective inhibition of hBChE over hAChE with excellent IC50 values .                                            Figure:  An overview of the workflow in this project. Isolation, followed by structure optimization, yields a                molecule capable of inhibiting hBChE at a nanomolar concentration.                 Indeed, the outstanding activities associated with the relatively simple scaffold motivated us to                optimize the structure to achieve enhanced hBChE inhibition (Figure). This endeavor resulted                                                                             2                in the synthesis of a derivative with IC50 hBChE = 3.8 ± 0.2 nM .                 References:                1.     Al Mamun, A., Maříková, J., Hulcová, D. et al. Amaryllidaceae alkaloids of belladine-type from Narcissus                pseudonarcissus  cv.  Carlton  as  new  selective  inhibitors  of  butyrylcholinesterase. Biomolecules 2020, 10,  800.                https://doi.org/10.3390/biom10050800                2.     Pidany, F., Kroustkova, J., Al Mamun, A., et al. Highly selective butyrylcholinesterase inhibitors related                to Amaryllidaceae alkaloids – Design, synthesis, and biological evaluation. Eur. J. Med. Chem. 2023, 252, 115301.                https://doi.org/10.1016/j.ejmech.2023.115301                                                                                                              54","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL22                     NMR-based metabolomics and anti-leukemic activity of plants used in                                          traditional medicine in Botswana                                                                                                1                      Chidoh Kootlole *, Vincenzo Carafa , Giulia Verrilli , Antonio Fiorentino , Lucia                                                                          2                                      1,                                                         2,3                                             Altucci 2,3,4 , Monica Scognamiglio 1                   1  Department of Environmental Biological and Pharmaceutical Sciences and Technologies,                       University of Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100, Caserta, Italy                    2  Department of Precision Medicine, University of Campania “Luigi Vanvitelli”, L. De                                              Crecchio 7, 80138 Naples, Italy                       3  Biogem Institute of Molecular and Genetic Biology, 83031 Ariano Irpino, Italy                 4  IEOS, Institute for Endocrinology and Oncology “Gaetano Salvatore”, 0131 Naples, Italy                                   *Corresponding author: chidoh.kootlole@unicampania.it                 Throughout the years, there have been great advancements in cancer medicine with an effort to                treat leukemia. Regardless of these several immunotherapies, leukemia remains the leading                cause of cancer related deaths in the world. Some of these therapies are associated with adverse                effects,  as  well  as  surfacing  of  drug  resistance.  Therefore,  continuous  search  for  new                compounds remains crucial.                With the help of NMR-based metabolomics, this study aims at identifying compounds from                selected  Botswana  plants  and  further  evaluating  their  anti-leukemic  activity.  Through                ethnopharmacological knowledge, nine plant species from different families were identified                and extracted. The extracts were subjected to NMR analysis for their chemical characterization.                Partial purification of the plants’ crude extracts using amberlite XAD-4 and XAD-7 was done                using water and methanol as eluents. The effects of the obtained methanol fractions on cell                viability, cell cycle and cell death in human U937 leukemia cell line were examined using flow                cytometry-based assays. Following the treatment, induction of cell death and strong modulation                of cell cycle was observed, particularly with fractions deriving from Maytenus senegalensis,                Elaeodendron transvaalense, and Ozoroa paniculosa. The NMR profiles showed that these                fractions were characterized by the presence of phenolic compounds, terpenoids, and alkaloids.                Further experiments are ongoing to obtain pure compounds, including their structures, from the                most active extracts.                                                                                                                               55","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL23                   Selective isolation and concentration of oleuropein and elenolic acid from                                     Olea europaea L. aqueous leaves extracts                                            1,                      Chiara Collevecchio *, Serena Fiorito , Francesco Epifano , Salvatore Genovese                                                           1                                                                               1                                                                                                    1                   1  Department of Pharmacy, University of study “G. D’annunzio” of Chieti-Pescara, Via dei                                            Vestini 31, 66100 Chieti (CH), Italy                                    *Corresponding author: chiara.collevecchio@unich.it                 The cultivation of olive trees and the production of olive oil generate massive amounts of waste.                For  this  reason,  the  valorization  of  by-products  deriving  from  Olea  europaea  L.  (Fam.                                                                         1                Oleaceae), has been a topic of great interest in recent years . Leaves, the most abundant waste,                are  already  widely  known  as  remedies  for  infections,  hypertension,  arrhythmia,  intestinal                spasms, cancer, and many others and thus their extract is used as main ingredient of several                pharmaceutical products. Indeed, they are rich in health-promoting functional components, of                which  the  secoiridoid  glycoside  oleuropein  is  one  of  the  most  abundant,  together  with  its                aglycones hydroxytyrosol (HT) and elenolic acid (EA), the latter less biologically studied until                    2                now .                To this regard, a microwave assisted procedure was performed to obtain an aqueous extract with                a high content in oleuropein. Subsequentially the active compound was selectively isolated                through solid phase adsorption (SPA) with the use of lamellar solids, powerful tools to enhance                the  chemical  stability  of  the  molecule,  and  surely  facilitating  its  use  in  olive-leaf  based                phytopreparations. Finally, an eco-friendly, readily available, and reusable catalyst like H2SO4                supported on silica was applied for the hydrolysis of oleuropein into its two main components.                An extract enriched in the monoaldehydic dihydropyran form of EA was obtained by an acid-                base work-up and this was later on characterized by GC-MS. A semipreparative scale HPLC                method was also validated for the final isolation of EA.                 References:                1.     Calvano,  C.  D.,  Tamborrino, A.  Valorization  of  Olive  By-Products:  Innovative  Strategies  for  Their                Production, Treatment and Characterization. Foods. 2022, 11, 768. https://doi.org/10.3390/foods11060768                2.     Selim, S., Albqmi, M., Al-Sanea, M.M., Alnusaire, T.S, Almuhayawi, M.S., AbdElgawad, H., Jaouni,                S.K.,  Warrad,  M.,  El-Saadony,  M.T.  Valorizing  the  usage  of  olive  leaves,  bioactive  compounds,  biological                activities,   and   food   applications:   A   comprehensive   review. Front   Nutr. 2022, 9,   1008349.                https://doi.org/10.3389/fnut.2022.1008349                                                                                                                           56","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL24                   Unprecedented phenolic compounds from Prospero autumnale (L.) Speta                                           with in vitro cytotoxic activities                                       1,                                                                                  4                                                                                                   1                                                    2                                                                      3                 Hasan Kırmızıbekmez *, Başak Aru , Jana Křoustková , Jiří Kuneš , Murat Erdoğan , Lucie                                                        Cahlíková 3                   1  Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, TR-34755, Kayışdağı,                                                      İstanbul, Türkiye                    2  Department of Immunology, Faculty of Medicine, Yeditepe University, TR-34755, Kayışdağı,                                                      İstanbul, Türkiye                     3  Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles                              University, Heyrovskeho 1203, Hradec Kralove, 500 05, Czech Republic                    4  Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy, Charles University,                                   Heyrovskeho 1203, Hradec Kralove, 500 05, Czech Republic                                       *Corresponding author: hasankbekmez@yahoo.com                 In  our  previous  study,  we  reported  the  isolation  of  six  secondary  metabolites  (1-6)  from                P. autumnale . As a part of our ongoing study on the same species, we aimed to isolate further                             1                secondary  metabolites  and  evaluate  the  in  vitro  cytotoxic  activities  of  all  compounds.                Phytochemical studies on the EtOAc fraction of the EtOH extract prepared from the bulbs led                to  the  isolation  of  a  new  homoisoflavonoid,  3R-(4′-hydroxybenzyl)-5,6,8-trihydroxy-7-                methoxy-4-chromanone (7), a new dimeric homoisoflavonoid (8) and a novel homoisoflavone-                stilbene type heterodimer (9). Chemical structures of the isolates were determined by NMR and                HR-MS analyses. This is the first report on the isolation of a homoisoflavone-stilbene type                heterodimer as well as a dimeric homoisoflavonoid from a natural source. The in vitro cytotoxic                activities  of  all  compounds  (1-9)  were  evaluated  against  colon  (HCT116,  LoVo),  prostate                (DU145, PC3), liver (HEP3B, HEPG2) and breast (MCF7, MDA-MB-231) cancer cell lines by                MTS assay. Compounds 1-3 and 9 exhibited potent cytotoxicity towards one or more cell lines                (IC50: 8.2-36.7 µM). Furthermore, these compounds were shown to induce apoptosis, necrosis                and cell cycle arrest showing variation depending on the cancer cells.                                Reference:                1.     Kırmızıbekmez,  H., Aru,  B.,  Křoustková,  J.,  Kuneš,  J.,  Erdoğan,  M.,  Cahlíková,  L.  Stilbenes  and                Homoisoflavonoids  from  Prospero  autumnale,  PSE  Natural  drug  discovery:  Current  approach  and  future                perspectives, October 12-15, 2023, Antalya-Türkiye. https://www.pse2023.org                                                                                                               57","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL25                 Comparative phytochemical analysis and bioactivity evaluation of Castanea                        sativa, Salix and Quercus spp. flower pollen and their bee pollen                                           1,                           Nisa Beril Sen *, Irena Vovk , Hasan Kırmızıbekmez , Etil Guzelmeric 1                                                                              1                                                       2                 1  Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Atasehir, Istanbul                                                      34755, Türkiye                  2  Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000                                                    Ljubljana, Slovenia                                    *Corresponding author: nisaberil.sen@yeditepe.edu.tr                 In Türkiye and Slovenia, apitherapy has been traditionally practiced for the prevention and/or                treatment  of  diseases  for  many  years  by  using  various  bee  products  (bee  pollen,  propolis,                honey). Among them, bee pollen is produced from pollen grains found in anthers of flowering                plants. Therefore, it should be taken into account that the chemical composition of bee pollen,                                                                                                    1                which is directly linked to its main floral source, is responsible for its health benefits . This                study  aims  to  compare  chemical  compositions  and  bioactivity  profiles  of  hydroalcoholic                extracts of Castanea sativa, Salix sp., and Quercus sp. flower pollen grains and their bee pollen                samples  obtained  from  Türkiye  and  Slovenia.  Firstly,  the  botanical  origins  of  bee  pollen                samples were evaluated by palynological analysis and HPTLC. After, fingerprinting profiles                were comparatively evaluated and the dominant and common compound in all samples was                purified by successive chromatographic methods and its structure was elucidated as N ,N ,N -                                                                                                     5                                                                                                        10                                                                                                  1                tricaffeoyl spermidine (TCS) by NMR and HRMS analyses. Besides TCS, other compounds                rutin, hyperoside, quercetin, quercitrin, isoquercitrin, and myricitrin were determined in flower                pollen  grains  and  quantified  in  all  samples  by  using  a  validated  UPLC  method.  Then,                standardized hydroalcoholic extracts were evaluated for their antioxidant and xanthine oxidase                (XO) inhibitory activities by  in-vitro tests and bioautography analyses (HPTLC-DPPH and                HPTLC-XO). C. sativa flower pollen and bee pollen showed the highest antioxidant and XO                inhibitory activities. Moreover, TCS was found to be the most bioactive compound that may be                responsible for the pharmacological activities of the extracts. Standardized C. sativa bee pollen                extract over TCS could have potential to be used as a food supplement in apitherapy.                 Acknowledgements: This study was supported by Yeditepe University Research Projects and                Scientific  Activities  Support  (YAP;  Grant  No:  YAP-US-SAB-22026),  The  Scientific  and                Technological  Research  Council  of  Türkiye  (TÜBİTAK;  Project  No:  119N569)  and  the                Slovenian  Research  and  Innovation Agency  (research  core  funding  No.  P1-0005  and  the                bilateral project BI-TR/20-23-004).                 References:                1.     Sen, N. B.; Guzelmeric, E.; Vovk, I.; Glavnik, V.; Kırmızıbekmez, H.; Yesilada, E. Phytochemical and                bioactivity  studies  on  Hedera  helix  L.  (Ivy)  flower  pollen  and  ivy  bee  pollen.  Antioxidants  2023,  12,  1394.                https://doi.org/10.3390/antiox12071394                                                                                                               58","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL26                    Anti-inflammatory properties of jabuticaba peel bioactive compounds                               during in vitro gastrointestinal and colonic digestion                                                                      3                                                                                                        2,4                                1,                                                                                   3                                                   2                Livia Reguengo *, Ana Sofia Saliba , Mateus Salgaço , Katia Sivieri , Severino de Alencar ,                                                 Mário Roberto Maróstica                                                                         1                   1  Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil                 2  Centro de Energia Nuclear na Agricultura, Universidade de São Paulo (USP), Piracicaba,                                                         SP, Brazil                   3  Postgraduate Program of Food and Nutrition, School of Pharmaceutical Sciences, State                                  University of São Paulo (UNESP), Araraquara, SP, Brazil                   4  Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo (USP),                                                   Piracicaba, SP, Brazil                                        *Corresponding author: liviareguengo@gmail.com                 Inflammation involves multiple processes in response to harmful stimuli, such  as bacterial                metabolites  (lipopolysaccharide),  producing  cytokines  and  pro-inflammatory  biomarkers.                Therefore, the inhibition of the inflammatory cascade response could attenuate this unbalanced                status. Vegetable food rich in polyphenols are correlated to health benefits in many studies,                especially  for  their  anti-inflammatory  potential.  Notwithstanding,  the  potential  effects  of                bioactive  compounds  are  directly  correlated  to  their  bioavailability  during  digestion.                Jaboticaba, also known as “Brazilian berry”, is a small fruit with a dark purple skin, normally                discarded, which is rich in anthocyanins, tannins and phenolic acids. Despite jaboticaba peel                (JP) health benefits being extensively reported in literature, the bioavailability of its bioactive                compounds is not always considered. Therefore, the objective of this work was to evaluate the                influence  of  the  digestion  of  JP  in  a  Simulator  of  Human  Intestinal  Microbial  Ecosystem                (SHIME) on its phenolic composition, antioxidant capacity and anti-inflammatory effects in                RAW 264.7 macrophage cell line. Antioxidant capacity analysis presented a slight divergence                but were in unison for JP extract superiority to stomach and, especially, duodenum values.                However, FRAP presented lower antioxidant results for colonic samples than JP extract, while                colonic samples results for ORAC overcame JP extract. As expected, monomeric anthocyanins                are degraded in stomach, and in duodenum in a greater extent, but there is a small recovery on                colonic samples, followed by a significant decay after 7  hours of  colonic fermentation. JP                extract (500 μg/mL) effectively reduced NF-κB, TNF-α and MIP-2 levels by 94.2%, 78.6% and                94.2%, respectively. Most of the samples have shown valuable TNF-α modulation, with the                lowest inhibition at 10%. However, NF-κB and MIP-2 could only be modulated to a certain                extent after JP digestion, being only stomach and duodenum samples effective regarding NF-                κB, whereas reductive effects could be observed in MIP-2 until 8 hours of digestion.                  Acknowledgements: This study was financed in part by the Coordenação de Aperfeiçoamento                de Pessoal de Nível Superior - Brasil (CAPES) -Finance Code 001; CNPq (307314/2023-5;                403976/2021-9; 301496/2019-6) and FAPESP (2022/09493-9; 2019/25048-2).                                                                                                                59","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL27                 Pulse Electric Field (PEF) treatment as innovative approach for milking the                           plant roots; advantage and disadvantage of this technique                                 Sylwester Ślusarczyk *, Kajetan Grzelka , Adam Matkowski                                                                                          1,2                                                    1,                                                                       1                    1  Department of Pharmaceutical Biology and Biotechnology, Wroclaw Medical University,                                     wyb. Ludwika Pasteura 1, 50-367 Wrocław, Poland                            2  Botanical Garden of Medicinal Plants, Wroclaw Medical University,                                   al. Jana Kochanowskiego 10, 51-610 Wrocław, Poland                                  *Corresponding author: sylwester.slusarczyk@umw.edu.pl                 The  aim  of  this  work  was  to  develop  and  optimize  PEF  treatment  for  the  reversible                electroporation from the roots and rhizome of 3 model plants Iris domestica (syn. Belamcanda                chinensis L. DC., Iridaceae) Scutellaria baicalensis L. and Cicerbita alpina (L.), all cultivated                in  aeroponic  system.  In  available  literature   is  improved  that  electroporation  can  be                                                             1,2                successfully used in extraction of compounds from plant tissue. In our work we expand on these                findings and further optimize the treatment parameters.  3 months old aeroponic cultivar of                model plants were electroporated using different pulsed electric field strength from 0,2 to 7.5                kV/cm with constant 50 or 100 μs duration of pulse and repetition N 50, 100 and 200 pulses.                We successfully extracted secondary metabolites after PEF, treated plants survived this process                and it can be regularly repeated on the same individual plants. The most promising  variant was                when we applied: E = 0.5 kV/cm N = 100 f = 1 Hz t = 100 us (A) and E = 0.5 kV/cm N = 200                f = 10 Hz t = 50 us (B) For extraction following solvents were used: choline chloride : glucose                (1:2) + 30% water; choline chloride : ethylene glycol (1:2); choline chloride : fructose (1:2) +                30% water; choline chloride : saccharose (1:2) + 40% water and tap water. After PEF plants                were cultivated in aeroponic systems and observed throughout the next 4 months, compared to                control. For HPLC-MS analysis all collected samples were first purified on SPE system to 10                mg/ml  concentration.  Main  disadvantage  is  frequent  infection  of  aeroponic  cultivars  with                typical pests and frequent fungal and bacterial overfeeding, which must be combated.                 Acknowledgements:  The  research  was  funded  in  whole  of  the  National  Science  Center                POLAND  basis  of  the  decision  number  National  Science  Centre  Poland;  UMO-                2020/39/D/NZ9/01402                  References:                1.     Kim  Y.  N.,  Kwon  H.  J.,  and  Lee  D.  U.,  “Effects  of  pulsed  electric  field  (PEF)  treatment  on                physicochemical  properties  of  Panax  ginseng,”  Innov.  Food  Sci.  Emerg.  Technol.  2019,  58,  1-7.                https://doi.org/10.1016/j.ifset.2019.102232                2.     Fincan M., DeVito F., and Dejmek P., “Pulsed electric field treatment forsolid-liquid extraction of red                beetroot pigment,” J. Food Eng., 2004, 64, 381–388. https://doi.org/10.1016/j.jfoodeng.2003.11.006                                                                                                                 60","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL28                  Originality of wild hops from northern France - a chemosensory approach                                                                 2                   Augustin Bildstein *, Mathilde Vandenberghe , Anne-Sophie Paguet , Gabriel Lefèvre ,                                     1,                                                                                                       1                                                                                     1                                                                                                       3                                  2                                                                  1                                                                                      1                                                 1                  Louis Raymond , Gaëtan Kientz , Franck Michels , Jennifer Samaillie , Séverine Derbré ,                               1                                                4                                                                                                1                                                                          1                 Cécile Palaric , David Mathiron , Marie-Laure Fauconnier , Jean-Xavier Fontaine , Rolland                                         Molinié , Sylvie Chollet , Céline Rivière 1                                                                 2                                                 1                  1  UMRt 1158 BioEcoAgro, Univ. Lille, JUNIA, UPJV, Univ. Liège, INRAE, Univ. Artois, Univ.                  Littoral Côte D’Opale, ICV—Institut Charles Viollette, F59650 Villeneuve D’Ascq, France                          2  ICL, Junia, Université Catholique de Lille, LITL, F-59000 Lille, France                 3  Université d’Angers, SONAS, SFR QUASAV, Faculty of Health Sciences, Dpt Pharmacy, 28                                          rue Roger Amsler, 49045 Angers, France                    4  Université de Picardie Jules Verne, Plateforme analytique, Rue Dallery – Passage du                                        sourire d'Avril, 80039 Amiens cedex, France                                  *Corresponding author: augustin.bildstein.etu@univ-lille.fr                 Humulus lupulus L., or hops, is a key ingredient used in brewing as an antiseptic, bittering agent                and flavouring agent. In recent years, brewers have been looking for new hop varieties with                original aromatic profiles. In our local context, a study highlighted the genetic and chemical                diversity of wild hops harvested in northern France, suggesting that wild hops could constitute                a potential resource for the creation of new varieties. The present study aims to confirm their                brewing potential using a chemosensory approach. Twenty-nine wild accessions were collected                in northern France and replanted ex-situ in an experimental hop field. They were compared to                19 commercial varieties and two heirloom varieties. The demonstration of the phytochemical                diversity of wild hops was based on the composition of non-volatile compounds, in particular                of prenylated phenolic compounds, by targeted and untargeted analyses by UHPLC-UV and                UHPLC-HRMS/MS, as well as on the composition of volatile compounds by HS-SPME GC-                MS. In addition, experimental beers were brewed and hopped with 13 wild hops from our                collection, 14 cultivars and 2 heirloom varieties. Sensory analysis was carried out by an expert                panel of 19 people trained in the characterization of hopped beers. Wild hops from northern                France showed a lower cohumulone/total alpha acid ratio. In addition, several accessions stood                out both in chemical analysis, with high levels of aromadendrene, zingiberene and farnesene                derivatives, and in sensory analysis, on specific descriptors such as floral and yellow fruit. Some                wild hops were comparable to commercial varieties, showing strong brewing potential.                 Acknowledgments: The authors thank the Haut-de-France Region, the French Foundation of                Brewery and Maltery, Junia-ISA, the french inter-branch organization of hop “Interhoublon”,                the french brewery union “Syndicat des Brasseurs des Hauts-de-France” as well as the CPER                project “BiHauts Eco de France” (Netwhop project) for their financial contribution.                                                                                                                    61","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL29                     Application of Pulsed Electric Fields increases yield of flavonoids and                   enables their extraction from Scutellaria baicalensis Georgi living roots                                                    2                Kajetan Grzelka , Joanna Jaśpińska , Adam Matkowski , and Sylwester Ślusarczyk *                                                                      1,2                                                                                                 1,                                1                    1  Dpt of Pharmaceutical Biology and Biotechnology, Div. of Pharmaceutical Biology and                  Botany, Wroclaw Medical University, wyb. Ludwika Pasteura 1, 50-367 Wrocław, Poland                            2  Botanical Garden of Medicinal Plants, Wroclaw Medical University,                                   al. Jana Kochanowskiego 10, 51-610 Wrocław, Poland                                  *Corresponding author: sylwester.slusarczyk@umw.edu.pl                 Scutellaria baicalensis Georgi (Lamiaceae) is an important medicinal herb. Under the name                Huang-Qin its rhizome has been used for centuries in Traditional Chinese Medicine. Nowadays                                                                             1                it’s  listed  in  Chinese,  European  and  British  Pharmacopoeias .  It  produces  many  phenolic                compounds, among which flavonoids (mainly baicalin, wogonoside, baicalein and wogonin)                are  dominant.  Thanks  to  these  substances,  root  extract  of  S.  baicalensis  has  cytotoxic,                                                                 2                anxiolytic, antioxidant and antimicrobial properties . Our aim was to use Pulsed Electric Fields                (PEF) in order to extract these secondary metabolites from living roots of S. baicalensis grown                in aeroponic cultures, and hopefully promote plant growth due to abiotic stress. In our studies                we’ve shown that most plants survive such treatment and keep on growing, reaching size and                content of root phytochemicals larger than control .                                                                3                In this experiment 6  months old  S. baicalensis specimens were electroporated in a special                cuvette filled with a conductive solvent. After 3 weeks of observation plants were harvested,                measured and dried. In most cases a significant increase in average mass and size of PEF-treated                plants’ roots was observed. Subsequently methanolic extracts of harvested roots were prepared.                We  found  that  the  extracts  were  synonymous  quality-wise,  but  differed  in  quantity  of                compounds when compared to control, in some cases exceeding the concentrations found in                this group. This highlights the potential of such electrostimulation procedure, which can be                further  developed  and  applied  to  virtually  any  other  plant  model  after  some  necessary                adjustments.                Acknowledgements:  This  research  is  funded  by  WMU  grant  to  Kajetan  Grzelka  no.                SUBK.D030.24.011, supported with the funds of the National Science Center POLAND basis                of the decision number National Science Centre Poland; UMO-2020/39/D/NZ9/01402                References:                1.     Wang,  Z. A  comprehensive  review  on  phytochemistry,  pharmacology,  and  flavonoid  biosynthesis  of                Scutellaria baicalensis. Pharm. Biol. 2018, 56, 465–484. https://doi.org/10.1080/13880209.2018.1492620                2.     Zhao,  T.  Scutellaria  baicalensis  georgi.  (Lamiaceae):  a  review  of  its  traditional  uses,  botany,                phytochemistry,  pharmacology  and  toxicology.  J.  Pharm.  Pharmacol.  2019,  71,  1353–1369.                https://doi.org/10.1111/jphp.13129                3.     Grzelka, K. Electrostimulation improves plant growth and modulates the flavonoid profile in aeroponic                culture   of   Scutellaria   baicalensis   Georgi.   Front.   Plant   Sci.   2023,   14:1142624.                https://doi.org/10.3389/fpls.2023.1142624                                                                                                               62","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL30                     Valorization of legume by-products for pharmaceutical, cosmetic, and                                              nutraceutical applications                                                    1,                                   Cristina Terenzi *, Franz Bucar , Vincenza Andrisano                                                                  2                                                                                       1                   1  Department for Life Quality Studies, University of Bologna, Corso D’Augusto 237, 47921                                                        Rimini, Italy                  2  Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz,                                           Beethovenstraße 8, 8010 Graz, Austria                                      *Corresponding author: cristina.terenzi2@unibo.it                 Approximately  5  billion  tons  of  biomass  residues  are  generated  annually  as  agri-food  by-                products globally. Among them, hulls, shells, peels, squeezing residues, and seed residues are                typically  discarded  as  waste,  leading  to  several  environmental  problems . Today,  food  by-                                                                                        1                products  generated  from  industrial  processes  are  considered  valuable  in  pharmaceutical,                 nutraceutical, and cosmetic fields mostly due to their high content of bioactive molecules, such                                                                    2                as polyphenols with a wide range of healing properties . Thus, a new idea of using by-products                as a source of bioactive, rather than discarding them, was born to reduce waste generation                following  circular  economy  principles.  This  research  aimed  to  investigate  the  phenolic                composition of different legume waste products supplied by a Romagna agri-food industry such                as soy, peas, green beans, and beans to valorise them. A polyphenol-rich liquid extract was                obtained from legume samples by a suitable Ultrasound-Assisted Extraction (UAE) method.                Then, a thorough analysis of polyphenols in UAE extracts was conducted using UHPLC-DAD-                ESI-MS .  After  meticulously  comparing  the  phenolic  profile  of  legume  waste  to  that  of                        n                commercially  available  final  products,  it  was  proved  that  by-products  can  be  effectively                considered a valuable resource. Then, the possible biological activities were explored to assign                different applications of each by-product UAE extract. Interesting results were obtained for                bean by-products containing glucaric acid derivatives, which have been shown to significantly                reduce  blood  cholesterol  levels .  Results  confirmed  that  the  agri-food  by-products  can                                                3                potentially  be  exploited  as  a  promising  source  of  bioactive  ingredients  to  design  new                formulations  with  a  wide  range  of  applications  in  the  pharmaceutical,  cosmetic,  and                nutraceutical industries falling within a circular economy perspective.                 References:                1.     Torres-Valenzuela, L. S.; Ballesteros-Gómez, A.; Rubio, S. Green Solvents for the Extraction of High                Added-Value  Compounds  from  Agri-Food  Waste.  Food  Eng  Rev.  2020,  12  (1),  83–100.                https://doi.org/10.1007/s12393-019-09206-y                2.     Baiano, A. Recovery of Biomolecules from Food Wastes — A Review. Molecules 2014, 19 (9), 14821–                14842. https://doi.org/10.3390/molecules190914821                3.     Walaszek, Z.; Szemraj, J.; Hanausek, M.; Adams, A. K.; Sherman, U. D-Glucaric Acid Content of Various                Fruits and Vegetables and Cholesterol-Lowering Effects of Dietary d-Glucarate in the Rat. Nutrition Research                1996, 16 (4), 673–681. https://doi.org/10.1016/0271-5317(96)00045-0                                                                                                                63","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL31                       Establishment and elicitation of Limonium algarvense Erben callus                         cultures: Implications for primary and secondary metabolism                                                                            1                                                                                                 1,                        Leonardo Lescano , Zoltán Cziáky , Luísa Custódio , Maria João Rodrigues *                                         1                                                         2                  1  Centre of Marine Sciences (CCMAR/CIMAR LA), University of Algarve, Faculty of Sciences                and Technology, Ed. 7, Campus of Gambelas, University of Algarve, 8005-139 Faro, Portugal                 2  Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400                                                   Nyíregyháza, Hungary                                        *Corresponding author: mjrodrigues@ualg.pt                 Limonium  algarvense  Erben,  a  medicinal  halophyte,  holds  significant  pharmacological                promise, attributed to its rich repertoire of bioactive compounds. The objective of this study                was to establish robust callus cultures as a sustainable, in vitro model for studying the plant's                metabolic responses, particularly focusing on the synthesis and accumulation of primary and                secondary metabolites under various elicitation treatments. Callus cultures were initiated from                leaf explants on Murashige and Skoog's medium supplemented with 1 mg/L picloram for 4                weeks. Afterwards, callus cultures were subjected to two elicitor treatments, including salicylic                acid–SA and yeast extract–YE at 50 and 100 mg/L for four weeks. Water extracts were assessed                for  their  shifts  in  primary  (total  soluble  sugars  and  proteins,  and  proline),  and  secondary                metabolism  (total  phenolics,  flavonoids,  and  condensed  tannins).  In  addition,  a  detailed                metabolic  profiling  was  conducted  using  high-performance  liquid  chromatography  with                electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). Elicitation induced significant                shifts  in  the  metabolite  synthesis  of  elicited  cultures  compared  to  controls.  While  YE50                markedly increased the callus yield, the total levels of phenolics, flavonoids condensed tannins                and total soluble proteins, the SA50 led to the highest increase in proline content. Metabolomic                analysis identified 10 metabolites, including gluconic acid, pyridoxine, and p-coumaric acid,                that  were  found  across  all  cultures.  Specific  elicitation  treatments  resulted  in  unique                metabolomic  profiles;  for  instance,  uralenneoside  and  riboflavin  were  missing  in  control                samples,  while  SA50  treatment  led  to  the  exclusive  presence  of  hydroxybenzoic  acid  O-                hexoside  and  the  absence  of  seven  other  compounds.  This  suggests  that  elicitation  can                effectively enhance the biosynthesis of primary and secondary metabolites in  L. algarvense                callus cultures, offering great potential for medicinal applications.                 Acknowledgements: This research was funded by Portuguese national funds from the FCT -                Foundation  for  Science  and  Technology,  through  projects  UIDB/04326/2020,                UIDP/04326/2020,  LA/P/0101/2020.  MJR  benefited  from  an  FCT  program  contract                (UIDP/04326/2020),  and  LC  was  supported  by  the  FCT  Scientific  Employment  Stimulus                (CEECIND/00425/2017).                                                                                                                  64","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL32                    Cosmetic applications of essential oils extracted from indigenous forest                                                   species of Taiwan                                                          1,                                                                                 2                      Senthil Kumar Kanthasamy Jayabal *, Gokila Vani Muthuraj , Sheng-Yang Wang     2                   1  Bachelor Program of Biotechnology and Center for General Education, National Chung                                            Hsing University, Taichung, Taiwan                        2  Department of Forestry, National Chung Hsing University, Taichung, Taiwan                                   *Corresponding author: zenkumar@dragon.nchu.edu.tw                 Essential oils, also known as plant volatiles, have garnered attention in cosmetic applications                due to their array of beneficial properties, including their aromatic fragrances and potential                therapeutic effects on the skin and hair. These oils serve as natural sources of biologically active                ingredients, offering a broad spectrum of bioactivities such as antioxidant, anti-inflammatory,                dermatoprotection, antiaging, and antimicrobial properties, thus becoming integral components                of herbal cosmetic products. Taiwan's rich forest biodiversity hosts over 5000 known native                species of flora. Our ongoing research focuses on exploring the skin health benefits of essential                oils  extracted  from  indigenous  forest  species  of Taiwan.  In  our  investigations,  we  initially                screened  the  anti-melanogenic  properties  of  16  essential  oils  extracted  from  native  forest                species in Taiwan, along with 31 commercial essential oils. Among them, Alpinia nantoensis                                   1                                                                   2                leaf  and  rhizome,   Calocedrus  formosana  wood,   Pogostemon  cablin,  and  Glossogyne                tenuifolia,  along  with  their  bioactive  compounds,  exhibit  robust  melanin  and  tyrosinase                inhibitory activities in skin melanocytes. Furthermore, our studies have unveiled that these                essential oils not only inhibit cellular tyrosinase activity but also modulate melanin biosynthesis                pathways.  Additionally,  we  have  discovered  that  Glossogyne  tenuifolia  essential  oil  and                limonene  from  Alpinia  nantoensis  essential  oil  protect  skin  keratinocytes  from  ultraviolet                                                                 3                irradiation-induced photodamage and photoaging.  Collectively, our findings underscore the                potential  of  essential  oils  as  promising  natural  sources  for  the  development  of  skin                whitening/lightening and anti-aging agents for cosmetic applications.                Acknowledgements: Authors thank the National Science and Technology Council, Taiwan for                supporting this study. Grant No. NSTC 111-2313-B-005-052-MY3. Title of project “The effects                of essential oils from native trees of Taiwan on premature skin aging and hyperpigmentation                and the modulatory effects of skin microbiota”.                 References:                1.     Kumar,  K.J.S.  et al.  Essential  oils  of  Alpinia  nantoensis  retard  forskolin-induced  melanogenesis  via                ERK1/2-mediated      proteasomal      degradation     of      MITF. Plants. 2020, 9,   1672.                https://doi.org/10.3390/plants9121672                2.     Hsiao, W.W. et al. Anti-melanogenic activity of Calocedrus formosana wood essential oil and its chemical                composition analysis. Plants. 2021, 1, 62. https://doi.org/10.3390/plants11010062                3.     Kumar, K.J.S. et al. Limonene protects human skin keratinocytes against UVB-induced photodamage and                photoaging by activating the Nrf2-dependent antioxidant defense system. Environ Toxicol. 2022, 37, 2897-2909.                https://doi.org/10.1002/tox.23646                                                                                                               65","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL33                                Chasing molecules - a structure elucidation journey                                                 1                   Jonas Kappen , Tristan Fuchs , S. Phani B. Vemulapalli , Lea Schmitz , Andrej Frolov ,                                 1                                                                         2                                                                                       1                                                                                                      1                                                                                5                                          Jeprianto Manurung 1,3,4 , Andria Agusta ,                                                                        2                                                 3,4                     Alexandra N. Muellner-Riehl , Christian Griesinger , Katrin Franke 1,4,6, *, Ludger A.                                                      Wessjohann 1,4, *                   1  Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry (IPB), Halle                                                     (Saale), Germany                    2  Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical                                              Chemistry, Göttingen, Germany                  3  Department of Molecular Evolution and Plant Systematics \& Herbarium (LZ), Institute of                                           Biology, Leipzig University, Germany                  4  German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Germany                    5  Research Center for Pharmaceutical Ingredients and Traditional Medicine, National                                Research and Innovation Agency (BRIN), Jakarta, Indonesia                   6  Institute of Biology/Geobotany and Botanical Garden, Martin Luther University Halle-                                                Wittenberg, Halle, Germany                          *Corresponding authors: kfranke@ipb-halle.de; wessjohann@ipb-halle.de                 The  newly  discovered  natural  compound  lumnitzeralactone,  identified  as  an  ellagic  acid                derivative,  was  isolated  from  the  antibacterial  extract  of  the  Indonesian  mangrove  species                Lumnitzera racemosa Willd. The structure of lumnitzeralactone, characterized by a deficit in                protons and a complex  condensed aromatic ring system, was unequivocally determined by                comprehensive spectroscopic analyses, including high-resolution mass spectrometry (HRMS),                    1                1D  H and  C nuclear magnetic resonance spectroscopy (NMR), and 2D NMR techniques,                            13                including the more unusual experiments 1,1-ADEQUATE and 1,n-ADEQUATE. The structural                elucidation was further supported by computer-assisted structure elucidation (CASE system                utilizing ACD-SE), density functional theory (DFT) calculations, and finally by  a two-step                chemical synthesis.                 Acknowledgements:  Financial  support  was  provided  by  the  project  “Indonesian  Plant                Biodiversity  and  Human  Health—BIOHEALTH”  (BMBF  grants  no.  16GW0120K  and                16GW0123),  the  project  ProCognito  by  EFRE  and  the  state  Saxony-Anhalt                (ZS/2018/11/95581)  and  by  the  German Academic  Exchange  Service  (DAAD  project  no.                91653688)                 References:                1.     Kappen et al. Challenging Structure Elucidation of Lumnitzeralactone, an Ellagic Acid Derivative from                the Mangrove Lumnitzera racemosa. Mar. Drugs 2023, 21, 242. https://doi.org/10.3390/md21040242                                                                                                                 66","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL34                   Extraction of spilanthol from Acmella oleracea with natural deep eutectic                                                         solvents                                                                                         2                                                                          1                                                         1                                                                                                     1                                    1,                     Fabian Alperth *, Theresa Feistritzer , Melanie Huber , Olaf Kunert , Franz Bucar                   1  Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz,                                           Beethovenstraße 8, 8010 Graz, Austria                 2  Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University                                      of Graz, Universitätsplatz 1, 8010 Graz, Austria                                     *Corresponding author: fabian.alperth@uni-graz.at                 Green chemistry approaches gain more and more importance in the field of natural product                research. The use of natural deep eutectic solvents (NADES), eutectic mixtures of hydrogen                bond donors and acceptors, as extraction agents for natural products has therefore become a                growing field of research . For the first time, 20 choline chloride-based NADES were tested                                         1-3                in comparison with ethanol as solvents for the extraction of spilanthol from Acmella oleracea                (L.) R.K.Jansen flower heads, analysed by HPLC-DAD quantification. Preceding UHPLC-                DAD-ESI-MS   analysis  as  well  as  isolation  using  a  dichloromethane  extract  revealed  the                              n                composition  of  Acmella  oleracea  extracts  and  yielded  spilanthol  along  with  further  main                constituents as reference compounds. The effects of water addition, time and temperature on                NADES extractions were also investigated. Best results in screening were achieved through                extractions with NADES consisting of choline chloride and 1,2-propanediol (1:2 molar ratio,                +20% water), comparable to ethanol yields. Choline chloride and methylurea (1:2, +20% water)                also showed promising results. These combinations were used for effect testing experiments, in                which time had the least effect on spilanthol yield, a raised temperature up to 80 °C increased                extraction, and water addition greater than 20% decreased spilanthol extraction. Since non-toxic                constituents were used for NADES preparations, promising extraction results could lead to                directly applicable formulations, among others for medicinal purposes.                  Acknowledgements: The authors acknowledge financial support by the University of Graz.                 References:                1.     Cannavacciuolo,  C.;  Pagliari,  S.;  Frigerio,  J.;  Giustra,  C.M.;  Labra,  M.;  Campone,  L.  Natural  Deep                Eutectic  Solvents  (NADESs)  Combined  with  Sustainable  Extraction  Techniques:  A  Review  of  the  Green                Chemistry Approach in Food Analysis. Foods 2022, 12, 56. https://doi.org/10.3390/foods12010056                2.     González-Laredo, R.F.; Sayago-Monreal, V.I.; Moreno-Jiménez, M.R.; Rocha-Guzmán, N.E.; Gallegos-                Infante, J.A.; Landeros-Macías, L.F.; Rosales-Castro, M. Natural deep eutectic solvents (NaDES) as an emerging                technology for the valorisation of natural products and agro-food residues: A review. Int. J. Food Sci. Tech. 2023,                58, 6660–6673. https://doi.org/10.1111/ijfs.16641                3.     Wawoczny, A.; Gillner, D. The Most Potent Natural Pharmaceuticals, Cosmetics, and Food Ingredients                Isolated  from  Plants  with  Deep  Eutectic  Solvents.  J.  Agric.  Food  Chem.  2023,  71,  10877–10900.                https://doi.org/10.1021/acs.jafc.3c01656                                                                                                                67","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL35                   Two new anthraquinones and other secondary metabolites from Asperula                                               sintenisii Achers ex Bornm                                            Ceren Öztürk*, Hasan Kırmızıbekmez                     Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, TR-34755,                                                Kayışdağı, İstanbul, Türkiye                                    *Corresponding author: ceren.ozturk@yeditepe.edu.tr                 Asperula  L.  is  one  of  the  foremost  genera  of  the  family  Rubiaceae,  which  comprises                approximately 200 species worldwide. It is represented by 41 species in the flora of Türkiye                with 49% endemism ratio. Some  Asperula species have been used as a diuretic, tonic and                against diarrhea in Anatolian folk medicine. Previous phytochemical investigations on Asperula                demonstrated that the genus contains anthraquinones, iridoids, flavonoids and phenolic acids                as the main secondary metabolites . A. sintenisii Achers ex Bornm is  a perennial subshrub                                                  1                endemic to Ida Mountains which has never been studied. In this study, we aimed to isolate and                identify the secondary metabolites from the whole plant of  A. sintenisii. The  air-dried and                powdered plant material was extracted with MeOH. The crude MeOH extract was suspended                in  H2O  and  submitted  to  liquid-liquid  extraction  with  n-hexane,  EtOAc  and  n-BuOH,                respectively. Sequential chromatographic separations on EtOAc and n-BuOH subextracts by                MPLC (C18 and SiO2) and Sephadex LH-20 afforded 14 secondary metabolites, including two                new  anthraquinones  (1  and  2)  along  with  five  known  iridoid  glycosides  (asperuloside,                deacetylasperuloside,  asperulosidic  acid,  deacetylasperulosidic  acid  and  geniposide),  four                flavonoids  (quercetin,  isoquercitrin,  rutin  and  kaempferol  3-O-rutinoside)  as  well  as  three                phenolic  acid  derivatives  (chlorogenic  acid,  ferulic  acid  4-O-β-D-glucopyranoside  and                rosmarinic acid). The chemical structures of purified compounds were elucidated on the basis                of extensive 1D and 2D NMR experiments and HR-MS analysis. Compounds 1 and 2 are rare                anthraquinone  derivatives  bearing  a  prenyl  group  cyclized  to  tetrahydrofuran  skeleton.                Moreover, geniposide, kaempferol 3-O-rutinoside and rosmarinic acid are being reported for                the first time from the genus Asperula.                               References                1.     Kırmızıbekmez, H., Tiftik, K., Kúsz, N., Orban‐Gyapai, O., Zomborszki, Z. P., Hohmann, J. Three new                iridoid  glycosides  from  the  aerial  parts  of  Asperula  involucrata.  Chem.  Biodivers. 2017,  14(3),  e1600288.                https://doi.org/10.1002/cbdv.201600288                                                                                                              68","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL36                   Integrating network pharmacology and experimental validation: In vitro                    assessment of neuroprotective effects and enzyme inhibition studies on                                Prunella vulgaris L. extract and isolated compounds                                             1,                                                                                   1                                                              1                       Vahap Murat Kutluay *, Zeynep Doğan  , Halime Eda Yalçın , İclal Saraçoğlu  1                  1  Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100, Ankara,                                                          Türkiye                                   *Corresponding author: muratkutluay@hacettepe.edu.tr                 Prunella vulgaris (PV) has been used traditionally for its anti-inflammatory effects and known                to be rich of rosmarinic acid (RA) . RA is frequently researched in recent years for Alzheimer's                                                 1                Disease (AD) treatment. However, the mechanism has not been elucidated yet. This study is                focused to predict potential targets-pathways of PV and isolated compounds in AD and support                these through in vitro assays.                RA, ursolic acid (UA) and rutin was isolated from the aerial parts of PV. RA content, the major                compound  of  PV,  was  determined  through  LC-MS  analysis  as  11.2%  (w/w).  For  network                pharmacology studies, target proteins of the compounds retrieved and GO and KEGG pathway                enrichment analysis performed. TNF, HIF-1, and TLR signalling pathways, and response to                LPS and H2O2 was determined as possible mechanisms of PV against AD. In vitro assays were                conducted parallel to network pharmacology studies. SOD and MDA levels in H2O2-induced                SH-SY5Y cells were evaluated and found that extract and compounds reduce oxidative damage                by  decreasing  MDA  levels.  UA  (100  μM)  was  identified  as  the  most  effective  compound                (reduction  of  91%). To  determine  neuroprotective  activity,  samples  were  applied  to  H2O2-                damaged SH-SY5Y cells and viability significantly increased even at 0.1 μM concentration for                all  compounds.  Rutin  showed  the  highest  activity. TNFα  levels  was  investigated  on  LPS-                stimulated BV2 cells, but samples didn’t show any significant change. In acetylcholine esterase                (AChE) and butyrylcholine esterase (BuChE) inhibition assays, PV and compounds showed                significant inhibition, with higher activity on BuChE. RA showed the highest activity for AChE                and  BuChE  (IC50  values  of  97.5  and  25.3  μM,  respectively).  Results  showed  that  PV  and                compounds showed significant neuroprotective activity and also showed AChE and BuChE                inhibitory activities that suggests as a potential for treatment of AD.                 Acknowledgements: Authors thank to The Scientific and Technological Research Council of                Türkiye (TUBITAK) (Project Number:121S491), and Hacettepe University Scientific Research                Project Coordination Unit (Project number THD-2023-20732).                 References:                1.     Pan, J., Wang H., Chen, Y. Prunella vulgaris L. – A Review of its Ethnopharmacology, Phytochemistry,                Quality   Control   and   Pharmacological   Effects.   Fron.   Pharmacol.   2022,   13:903171.                https://doi.org/10.3389/fphar.2022.903171                                                                                                               69","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL37                  Anti-inflammatory properties of biomolecules from the Euganean Thermal                                                         District                                                                                                        3                                   1,                                                                    2                                                                                       3                   Micol Caichiolo *, Raffaella Margherita Zampieri , Alessandra Adessi , Matilde Ciani ,                       Giuliana d’Ippolito , Fabrizio Caldara , Luisa Dalla Valle , Nicoletta La Rocca 1                                                            5                                                                              1                                          4                           1  Department of Biology, University of Padova, Via Ugo Bassi 58/b, Padua, Italy                    2  Research Institute on Terrestrial Ecosystems (IRET), CNR, Via Madonna del Piano 10, Sesto                                                 Fiorentino (Florence), Italy                  3  Department of Agriculture, Food, Environment and Forest (DAGRI), University of Florence, Via                                                Maragliano 77, Florence, Italy                   4  Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, Pozzuoli (Naples), Italy                        5  Pietro D’Abano Thermal Studies Center, Via Jappelli 5, Abano Terme (Padua), Italy                                   *Corresponding author: micol.caichiolo@phd.unipd.it                 The Euganean Thermal District (North-East Italy) is one of Europe’s largest and oldest thermal                centres. The Italian Health System acknowledges the use of its therapeutic thermal muds as a                beneficial treatment for patients with arthro-rheumatic diseases. Cyanobacteria represents one                of the most abundant Phyla among the microorganisms that constitute mud’s biofilms and are                known to produce complex polysaccharides and glycoglycerolipids with potential therapeutic                        1-3                efficacy . In this study, we assessed the anti-inflammatory activity of polysaccharides (PS)                extracted from muds matured at different temperatures in five spas of the Euganean Thermal                District,  as well as total  lipids (TL) extracted from the three  most abundant cyanobacteria                strains, cultivated under optimal conditions on a lab scale. PS were characterized by analyzing                the FT-IR spectrum, the zeta potential, the monosaccharide composition and the abundance of                sulfate groups. TL were characterized by analyzing the fatty acid composition and the relative                abundance of lipid classes. Subsequently, we evaluated recovery from an inflammatory state                using a zebrafish model of copper sulfate-induced inflammation. Following treatment with our                bioactive  compounds,  we  examined  the  recovery  of  the  developmental  delays  due  to  the                inflammatory state through morphometric analyses of swim bladder insufflation and operculum                bone  area.  The  results  indicate  that  all  PSs,  extracted  from  muds  matured  at  different                temperatures,  possess  chemical  composition  homogeneity  and  exhibit  similar  anti-                inflammatory  activity.  Additionally,  TL  also  presents  an  anti-inflammatory  potential.  In                conclusion, investigating the healing effects of compounds produced by the Euganean mud                microbiota represents a crucial step in the scientific validation of the Euganean mud therapies                and further supports their potential in the recovery from chronic inflammatory diseases.                 References:                1.     Zampieri, R.M.; Adessi, A.; Caldara, F.; Codato, A.; Furlan, M.; Rampazzo, C.; De Philippis, R.; La Rocca,                       N.; Valle, L.D. Anti-Inflammatory Activity of Exopolysaccharides from Phormidium Sp. ETS05, the Most                       Abundant  Cyanobacterium  of  the Therapeutic  Euganean Thermal  Muds,  Using  the  Zebrafish Model.                       Biomolecules 2020, 10, doi:10.3390/biom10040582.                2.     Zampieri, R.M.; Adessi, A.; Caldara, F.; De Philippis, R.; Dalla Valle, L.; La Rocca, N. In Vivo Anti-                       Inflammatory and Antioxidant Effects of Microbial Polysaccharides Extracted from Euganean Therapeutic                       Muds. Int. J. Biol. Macromol. 2022, doi:10.1016/J.IJBIOMAC.2022.04.123.                3.     Lalli, A.; Andreoli, C.; Ceschi Berrini, C.; De Appolonia, F.; Marcolongo, G. Anti-Inflammatory Active                       Principles in Euganean Thermal Mud. European Patent 1571203 (B1), 2013.                                                                                                              70","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                OL38                             Antioxidant activity and phenolic compounds contents of                                                  Tribulus terrestris L.                                    Derya Çiçek Polat *, Safa Gümüşok , Ceyda Sibel Kılıç                                                   1,                                                                     1                                                                                        1                   1  Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara,                                                          Türkiye                                        *Corresponding author: polatd@ankara.edu.tr                 Tribulus L. genus, belonging to Zygophyllaceae family, has about 20 species, but only Tribulus                terrestris grows naturally in Türkiye. It is known as \"demirdikeni, çobançökerten, çarıkdikeni,                or deveçökerten\" in Türkiye and used as infusion (5%) to treat kidney stones, as diuretic and                strengthener. Due to the traditional use of T. terrestris species, many biological activity studies                have been performed on the species such as anticancer, antioxidant, antimicrobial, analgesic,                anti-inflammatory, antiurolitic, antidiabetic, cardiotonic, aphrodisiac, and antispasmodic etc.                Phenolic compounds that are found in plants have significant biological activities. Therefore, it                is important to determine the amount of these compounds.  In this study, amounts of some                                                                          1,2                phenolic compounds were determined on methanol extracts prepared from different parts of                 Tribulus terrestris (flowers, fruits and herb) collected from Dikili, Türkiye. In addition, total                phenolic and total flavonoid contents of extracts were determined as gallic acid and quercetin                equivalents, respectively. Antioxidant activities were carried out by two methods (DPPH free                radical scavenging capacity assay and ABTS radical cation radical scavenging assay).                As a result of HPLC analysis, chlorogenic acid, ferulic acid, gallic acid, and rutin were detected.                The highest amount of chlorogenic acid and gallic acid were determined as 0.75% and 0.15%                in the extract of the flowers, respectively. The highest amount of ferulic acid and rutin were                determined as 0.1% and 0.23% in the herb extract, respectively. Extracts of the flowers also had                the highest total phenolic content (35.03 mg GAE/g extract); on the other hand, extracts of the                fruits had the highest total flavonoid content (113.22 mg QE/g extract). In terms of antioxidant                activity, the herb extract (DPPH: 0.19 mg/mL (IC50); ABTS: 30.81 mg TE/g extract) was found                to be more active than the extracts prepared from the other parts of the plant.                 References:                1.     Çömlekçioğlu, N., Çırak, R. Comparison of fatty acid composition and antioxidant contents of Tribulus                Terrestris   L.   collected   from   different   localities.   TURJAF.   2021,   9(8),   1448-1454.                https://doi.org/10.24925/turjaf.v9i8.1448-1454.4273                2.     Ștefănescu, R., Tero-Vescan, A., Negroiu, A., Aurică, E., Vari, C. E. A comprehensive review of the                phytochemical, pharmacological, and toxicological properties of Tribulus terrestris L. Biomolecules. 2020, 10(5),                752. https://doi.org/10.3390/biom10050752                                                                                                                      71","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                List of Poster Presentations (Listed alphabetically)                 PO1     Miriam Bačkorová                         The pharmacological potential of the lichens Pseudevernia furfuracea (L.) Zopf.                         and Cladonia stellaris (Opiz) Pouzar \& Vězda                 PO2     Kateřina Berková                         Effect of subcritical water extraction fractionation on antioxidant activity and total                         phenolic content of teas and tea-like plants                 PO3     Monika Bielecka                         Expression analysis of ribosome-inactivating proteins and genes related to                         triterpenoid saponin biosynthesis in Agrostemma githago L.                 PO4     Silvia Bittner Fialová                         Phytochemical and antibacterial profile of Perilla frutescens L.                 PO5     Malwina Brożyna                         From koala snack to anti-microbial attack: Potency of 1,8-cineole against drug                         resistant microorganisms                 PO6     Beata Budíková                         Using mass spectrometry for study of salicylic acid metabolism in plants                 PO7     Kateřina Cermanová                         Quantification of Yang cycle intermediates and related compounds by LC-MS/MS                         in plants                 PO8     Luísa Custódio                         Functionalization of goat cheese with biomass and extracts from the halophyte                         species Helychrisum picardii picardii                 PO9     Luísa Custódio                         How ecotype influences the nutritional profile of the edible halophyte Sarcocornia                         perennis cultivated under saline conditions?                 PO10  Eva Čonková                         Comparison of antibiofilm efficacy of selected azole antifungals and plant essential                         components in Candida albicans strains                 PO11  Kamila Dokupilová                         Study of antimicrobial activity of Sorbus domestica L. leaf extract against                         Staphylococcus aureus                 PO12  Margita Dvorská                         Chromatographic separation of xanthones from Maclura pomifera                 PO13  Magdalena Fast                         Saccharides in Polygoni cuspidati rhizoma et radix decoction as biologically active                         compounds stimulating the proliferation of gingival fibroblasts                 PO14  Barbora Fiserova                         Susceptibility of intestinal bacteria involved in colorectal cancer pathogenesis to                         extracts from Philippine medicinal plant species and plant-derived compounds                 PO15  Mária Gáborová                         Antiproliferative diterpenes from three Plectranthus s.l. species                 PO16  Jiří Hanyk                         p-Topolin and its mesylate as possible active components of skin care products                 PO17  Patrícia Harčárová                         Iron and copper chelation by benzoic acids and their effect on metal catalyzed                         Fenton reaction and hemolysis                 PO18  Martin Helcman                         Evaluating of anti-quorum sensing activity of natural phenolic compounds                                                                                                                72","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 PO19  Pavel Hladík                         Auxin metabolism: unravelling novel pathways and metabolites by liquid                         chromatography-mass spectrometry analysis                 PO20  Jan Hošek                         Yeast glucan particles – bioactive drug vehicles                 PO21  Marketa Houdkova                         Liquid matrix volatilization methods for susceptibility testing of respiratory                         bacteria to volatile agents in liquid and vapour phase                 PO22  Subhasis Chattopadhyay                         Bioactive 3D supramolecular coordination architectures of bile acids                 PO23  Smiljana Janković                         Satureja montana and S. kitaibelii essential oil variability – chemotaxonomic                         approach                 PO24  Smiljana Janković                         The alkane profile as a significant tool for the chemotaxonomy of the genus                         Clinopodium L.                 PO25  Dagmar Jankovská                         Anticholinesterase activity of methanolic extract of Amorpha fruticosa flowers and                         isolation of putrescine and spermidine derivatives                 PO26  Jincheng Shen                         Exploring structure-activity-relationships of insect deterrent saponins                 PO27  Ondřej Jurček                         Steroidal molecules as building blocks in supramolecular and material chemistry                 PO28  Iva Jurčević Šangut                         Assessment of polyphenol variability, flavonoids, phenolic acids, pigments, and                         antioxidant activity across two growth stages of ginkgo leaves                 PO29  Didem Şöhretoğlu                         Assessment of cytotoxicity and antioxidant capacity of Ranunculus arvensis L. and                         isolation of its flavonoids                 PO30  Sylvia Kalli                         LC-(HR)MS/MS method to determine cucurbitacins in Cucurbitaceae – Survey in                         Dutch retail market                 PO31  Jonas Kappen                         Chasing molecules - interdisciplinary approaches in modern structure elucidation                 PO32  Ema Kostovčíková                         The study of the antibacterial activity of agrimony against gram-positive bacteria                 PO33  Weronika Kozłowska                         Isoquinoline alkaloids in Corydalis cheilanthifolia cultured in vitro                 PO34  Weronika Kozłowska                         In vitro culture and phytochemical profiling of Agrostemma githago and                         Gypsophila elegans                 PO35  Elena Kurin                         Oregano and red wine: an antioxidant combination that leads to synergy                 PO36  Slavomír Kurhajec                         Evaluation of Antidiabetic Properties of Roselle plant extracts (Hibiscus sabdarifa                         L.)                 PO37  Jovana Lisičić                         Evaluating the anti-inflammatory potential of prenylated (iso)flavonoids from Ficus                         cyathistipula in an in vitro model of inflammatory response                 PO38  Marta Lo Vetere                         Antimicrobial activity of two orange varieties essential oils for the valorisation of                         peel wastes                                                                                                            73","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 PO39  Zuzana Lomozová                         Constituents of hawthorn (Crataegus spp.) and their ability to inhibit intestinal α-                         glucosidases                 PO40  Agnieszka Łubek-Nguyen                         Anti-inflammatory activity and phytochemical profile of different plant material                         from Rhododendron luteum Sweet – comparative analysis                 PO41  Agnieszka Łubek-Nguyen                         Optimization of extraction of bioactive pentacyclic triterpenes and polyphenols                         from rose roots                 PO42  Milan Malaník                         α-Glucosidase inhibitory activity and phytochemical profile of fruit extracts of                         Czech Sorbus endemics                 PO43  Juraj Markuš                         Screening of Glycyrrhiza species for anti-inflammatory phenols                 PO44  Lenka Molčanová                         Phlorotannins from brown algae Ecklonia radiata (C.Agardh) J.Agardh – isolation,                         structural elucidation, and antiproliferative properties                 PO45  Izabela Nawrot-Hadzik                         Agrostemma githago as a rich source of flavonoid-C-glycosides and triterpene                         saponins                 PO46  Daniela Nykodýmová                         Determination of PPAR gamma agonistic activity of mimulone and diplacone in                         nanoparticle formulations                 PO47  Won Keun Oh                         Formulation with three herbs exhibits antiviral activity against highly pathogenic                         influenza a virus in chickens                 PO48  Bartosz Pencakowski                         Evaluation of reference gene expression stability by quantitative real-time PCR in                         Agrostemma githago                 PO49  Filip Pidaný                         Synthesis and screening of carltonine mimetics for selective butyrylcholinesterase                         inhibition                 PO50  Anna Prescha                         Colon-targeted delivery pomegranate and blackcurrant seed oils exhibiting                         antitumor activity                 PO51  Péter Püski                         Quality assessment of Boswellia serrata extract- containing food supplements on                         the Hungarian market                 PO52  Carlo Raucci                         2D- NMR chemical investigation of bioactive fractions of Clinopodium nepeta                 PO53  Maria João Rodrigues                         Nature versus cultivation: exploring the phytochemical diversity and bioactive                         potential of the halophyte Plantago coronopus L.                 PO54  Michal Rybnikář                         Isolation and identification of compounds from Schisandra chinensis fruit and                         effect of dibenzocyclooctadiene lignans on gap junction intercellular                         communication                 PO55  Chandrama Roy Chowdhury                         Effect of short-term apple consumption on fecal metabolites: A pre-post                         interventional study using NMR analysis                                                                                                                74","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 PO56  Katja Schoss                         Extraction of cannabinoids, volatile terpenes, and polyphenols from Cannabis                         sativa: subcritical water vs. supercritical CO2 and organic solvents                 PO57  Gabriela Škovranová                         Antimicrobial properties of phenolics against fusidic acid-resistant MRSA in wound                         healing                 PO58  Aleksander Smakosz                         Agrostemma githago (common corn-cockle) seeds as a new source of unsaturated                         fatty acids                 PO59  Ondřej Smištík                         Anti-inflammatory and antioxidant activity of dibenzocyclooctadiene lignans from                         Schisandra chinensis                 PO60  Angela Sorice                         Phytochemical profile of wild and cultivated Nigella damascena species collected                         in Sicily (Italy)                 PO61  Marta Stafiniak                         How flavonoid-C-glycosides are made in the Caryophyllaceae? – a contribution to                         biosynthesis-related genes discovery                 PO62  Miroslava Stankovianska                         Oncostatic activities of plant nutraceuticals in rodent models of breast carcinoma                 PO63  Mariam Taha                         Environmentally friendly solid-liquid extraction for the recovery of polyphenols                         from almond okara                 PO64  Jakub Treml                         Identification of plant phenolics as novel PPARγ agonists and hypoglycemic agents                 PO65  Giovanna Valentino                         Bioactive small molecules from Mediterranean plants as ligands for DNA                         secondary structures                 PO66  Hristo Vasilev                         Five new tamarixetin glycosides from Astragalus thracicus Griseb., including some                         substituted with the rare 3-hydroxy-3-methylglutaric acid and their collagenase                         inhibitory effects in vitro                 PO67  Rudolf Vrabec                         Vincarubine: Unraveling a cytotoxic mechanism against cancer cells                 PO68  Sutthida Wongsuwan                         Semi-targeted metabolomics of Selaginella plants based on LC-MS coupled with                         bioactivity assay for identification of biomarkers                 PO69  Iwan Zalewski                         The analysis of the in vitro flax plant with tocopherol-pathway gene VTE2                         overexpression                 PO70  Sylwia Zielińska                         Biotic stress as modulator of isoquinoline alkaloid profile in Chelidonium majus in                         hydroponic and in vitro cultures                 PO71  Marcel Białas                         Anti-Inflammatory Effect and Cytotoxic Properties of Glaucium flavum Extracts                 PO72  Bartłomiej Dudek                         Bug vs Bug: Use of Galleria mellonella larvae to test activity of Essential Oils and                         their components against drug-resistant microbes                                                                                                                  75","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO1                   The pharmacological potential of the lichens Pseudevernia furfuracea (L.)                                Zopf. and Cladonia stellaris (Opiz) Pouzar \& Vězda                                                                                             3                              Miriam Bačkorová *, Lucia Ungvarská-Maľučká , Eva Čonková                                                                             2                                                1,                    1  Department of Pharmaceutical Technology, Pharmacognosy and Botany, University of                            Veterinary Medicine and Pharmacy, 041 81, Košice, Slovak Republic                 2  Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine                                      and Pharmacy, 041 81, Košice, Slovak Republic                    3  Department of Pharmacology and Toxicology, University of Veterinary Medicine and                                         Pharmacy, 041 81, Košice, Slovak Republic                                      *Corresponding author: miriam.backorova@uvlf.sk                 Lichens are symbiotic organisms that are composed of a mycobiont (fungus) and one or more                photobionts (algae or cyanobacteria). The products of lichen secondary metabolism represent a                unique  group  of  natural  organic  substances  with  significant  pharmacological  activity.  The                present work evaluates the antiproliferative, antifungal, antiangiogenic and antioxidant effects                of lichen extracts Pseudevernia furfuracea (PSE) and Cladonia stellaris (CLA). The metabolic                activity of PSE and CLA extracts at different concentrations was determined by MTS assay on                four tumor cell lines. The lichen CLA extract significantly inhibited the metabolic activity of                the cells compared to the PSE extract. The antifungal activity of PSE and CLA extracts was                tested on three reference yeast strains. Candida albicans was the most sensitive to the PSE                extract with an average MIC of 0.16%. Candida parapsilosis and Malassezia pachydermatis                showed very good sensitivity to PSE and CLA.e. In terms of angiogenesis evaluation in the                CAM model, there was a significant inhibition of vessel density after application of CLA extract                at a concentration of 200 µg/ml. Total vessel length was not significantly affected and vessel                branching was significantly affected by PSE extract at  a concentration of 200 µg/ml. PSE                extract showed higher antioxidant activity compared to CLA. Based on the comparison of the                                                                           13                                                                    1                chemical shifts of hydrogen and carbon atoms in the  H and  C NMR spectra of the standard                and in the lichen extract samples, it can be concluded that atranorin was present in the PSE                extract and the presence of usnic acid was confirmed in the CLA extract.                 Acknowledgements: Authors thank to  Ministry of  Education KEGA, Grant No. 003UVLF-                4/2024,  Medicinal  mushrooms  in  pharmacy  and  building  3D  collections  of  medicinal                mushrooms                 References:                1.     Zhao, Y. et al. A comprehensive review on secondary metabolites and health-promoting effects of edible                lichen. J. Funct. Food. 2020, 80, 104283. http://doi: 10.1016/j.jff.2020.104283                                                                                                                  76","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO2                   Effect of subcritical water extraction fractionation on antioxidant activity                               and total phenolic content of teas and tea-like plants                                                   1,                                 Kateřina Berková *, Asad Walayat , Johana Rondevaldová                                                                                          1                                                                  1                    1  Laboratory of Ethnobotany and Ethnopharmacology, Department of Crop Science and                  Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague,                                   Kamýcká 129, 165 00 Praha – Suchdol, Czech Republic                                      *Corresponding author: berkovak@rektorat.czu.cz                 Tea (Camellia sinensis) and tea-like plants (e.g. maté – Ilex paraguariensis) are rich sources of                antioxidants. Unfortunately, methods used for their extraction are usually environmentally non-                friendly,  time-consuming,  and  with  low  extraction  efficiency.  Subcritical  Water  Extraction                (SWE) is a novel green method used for the extraction of natural products that can improve                heat  and  mass  transfer,  thus  increasing  extraction  yields  and  efficiency.  However,  the                methodology  for  SWE  extraction  of  beverage  plants  is  globally  not  uniform,  especially  in                temperature and extraction time, which strongly affect the extraction efficiency. In this study, 3                different plant beverages (green tea, black tea, maté) were extracted using the SWE method                where the extracts were collected in the form of time-separated fractions (1×10 min, 2×5 min                and 5×2 min). In  addition, as representative of the conventional extraction methods, water                infusion  was  also  performed.  Subsequently,  antioxidant  activity  (DPPH,  ORAC)  and Total                phenolic content (TPC) were evaluated in all samples to describe the best extraction conditions.                The strongest antioxidant activity and highest TPC in green and black tea were found in the 2nd                5-min fraction, where IC50 for DPPH was 9.733 µg/mL (green tea) and 16.23 µg/mL (black                tea), IC50 for ORAC was 6.503 µg/mL (green tea) and 6.4383 µg/mL (black tea), and TPC was                778.53 mg GAE/g (green tea) and 650.55 mg GAE/g (black tea). In the case of maté, the results                of DPPH and ORAC testing were not unequivocal, but the highest TPC was also found in the                2nd 5-min faction (540.314 mg GAE/g) and in addition in the 4th 2-min fraction (543.308 mg                GAE/g). These findings indicate that the chemical composition of the SWE extracts changes                during the extraction time and the fractionation is a powerful tool for achieving the strongest                possible extracts.                Acknowledgements: This research was financially supported by the Internal Grant Agency of                the Faculty of Tropical AgriSciences (IGA No. 20233109) and Foundation \"Nadání Josefa,                Marie a Zdenky Hlávkových\".                 References:                1.     Hiep, N. T.; Duong, H. T.; Anh, D. T.; Hoai Nguyen, N.; Thai, D. Q.; Linh, D. T. T.; Anh, V. T. H.; Khoi,                N. M. Subcritical Water Extraction Of Epigallocatechin Gallate From Camellia Sinensis And Optimization Study                Using Response Surface Methodology. Processes 2020, 8 (9). https://doi.org/10.3390/pr8091028.                2.     Essien, S. O.; Young, B.; Baroutian, S. Recent Advances In Subcritical Water And Supercritical Carbon                Dioxide Extraction Of Bioactive Compounds From Plant Materials. Trends Food Sci. Technol. 2020, 97, 156-169.                https://doi.org/10.1016/j.tifs.2020.01.014.                3.     Ko, M. -J.; Cheigh, C. -I.; Chung, M. -S. Optimization Of Subcritical Water Extraction Of Flavanols From                Green Tea Leaves. J. Agric. Food Chem. 2014, 62 (28), 6828-6833. https://doi.org/10.1021/jf5012006.                                                                                                                77","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO3                  Expression analysis of ribosome-inactivating proteins and genes related to                          triterpenoid saponin biosynthesis in Agrostemma githago L.                                                      1                                 1,                                                                           1                                                                                                 1                 Monika Bielecka *, Marta Stafiniak , Bartosz Pencakowski , Weronika Kozłowska , Izabela                  Nawrot-Hadzik , Fabian Bülow , Aleksander Weng , Christoph Weise , Adam Matkowski                                                                                                        1                                                                                     3                                                                   2                                                2                                 1                   1  Department of Pharmaceutical Biology and Biotechnology, Wroclaw Medical University,                                                     Wroclaw, Poland                   2  Department of Pharmaceutical Biology, Institute of Pharmacy, Freie Universität Berlin,                                                     Berlin, Germany                     3  Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany                                    *Corresponding author: monika.bielecka@umw.edu.pl                 The seeds of Agrostemma githago L (corn cockle, Caryophyllaceae) are toxic and poisonings                were frequent in the past from contaminated flour. The toxicity of corn cockle is attributed to a                toxic two component-system (TTS) which consists of type I ribosome-inactivating proteins                                                     1                (RIPs) and specific triterpene saponins . RIPs are N-glycosylases that inactivate the ribosomal                RNA, leading to an irreversible inhibition of protein synthesis and cell death. A. githago has                                              2                been found to produce agrostin , the type I RIP, and saponins such as gypsogenin and quillaic                acid glycosides. The biosynthesis of agrostin and saponins has not been studied in A. githago.                To get insight into the mechanisms regulating the TTS, we performed expression analysis of its                components in various tissues and organs at the transcription level. The plant material grown in                the experimental field of the Botanical Garden of Medicinal Plants (BGMP) at the Wroclaw                Medical University (Poland) and from in vitro cultures was used. Nucleotide sequences of genes                putatively involved in the biosynthesis of triterpenes and these encoding RIP were identified in                                                                  2                the A. githago transcriptome obtained by RNAseq . Selected sequences were used for PCR                primer design and the tissue-specific expression of genes encoding for: RIP, squalene synthase,                squalene monooxygenase, β-amyrin synthase as well as several cytochromes P450 and UDP-                dependent glycosyltransferases, was studied.                The analysis contributed to an elucidation of the unexplored toxic synergism within plants of                the carnation family. In a long run, our investigation may lead to an optimization of triterpene                saponin profile in A. githago and serve for further research in their biomedical applications.                 Acknowledgements: This research is funded by the National Science Center of Poland (NCN),                grant no. 2020/39/I/NZ7/01515 (RIPSAPO).                 References:                1.     Hebestreit, P.; Melzig, M. F. Cytotoxic Activity of the Seeds from Agrostemma githago var. githago.                Planta Med. 2003, 69, 921–925. https://doi.org/10.1055/s-2003-45101.                2.     Weise, C.; Schrot, A.; Wuerger, L. T. D.; Adolf, J.; Gilabert-Oriol, R.; Sama, S.; Melzig, M. F.; Weng, A.                An  Unusual  Type  I  Ribosome-Inactivating  Protein  from  Agrostemma  Githago  L.  Sci.  Rep.  2020,  10,                https://doi.org/10.1038/s41598-020-72282-2.                                                                                                               78","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO4                        Phytochemical and antibacterial profile of Perilla frutescens L.                                                              2                                      1,                                                                                   3                                                                                                        1                  Silvia Bittner Fialová *, Lívia Slobodníková , Svetlana Dokupilová , Kamila Dokupilová ,                                                                             1                                                               4                                                 1                                                                                            1                               Ema Kostovčíková , Iveta Čičová , Elena Kurin , Pavel Mučaji                    1  Comenius University Bratislava, Department of Pharmacognosy and Botany, Faculty of                                    Pharmacy, Odbojárov 10, 832 32 Bratislava, Slovakia                 2  Comenius University Bratislava, Faculty of Medicine, Institute of Microbiology, Sasinkova                                               4, 811 08 Bratislava, Slovakia                   3  Comenius University Bratislava, Faculty of Pharmacy, Department of Pharmaceutical                         Analysis and Nuclear Pharmacy, Odbojárov 10, 832 32 Bratislava, Slovakia                   4  National Agricultural and Food Centre, Research Institute of Plant Production, 921 01                                                     Piešťany, Slovakia                                      *Corresponding author: fialova@fpharm.uniba.sk                 Perilla frutescens L. is an aromatic medicinal plant widely used in food and as a medicine. Two                P.  frutescens  varieties  (green  and  purple)  were  examined  in  terms  of  antioxidant  and                antibacterial  action.  Phytochemical  analysis  was  performed  using  LC-MS,  HPLC,  and                spectrophotometry.  Of  tested  gram-positive  and  gram-negative  bacterial  strains,  the  most                susceptible in the microdilution test was methicillin-resistant Staphylococcus aureus (MRSA;                MIC/MBC 2.5 mg/mL). The antibacterial effect against MSSA/MRSA was further examined                using microcalorimetry (Fig. 1). In antioxidant activity testing by the DPPH method, the purple                variety of perilla was 3-fold stronger than the green one (IC50 DPPH 7.7 μg/mL). Phytochemical                analysis revealed the presence of phenolic compounds (phenolic acids and flavonoids), among                which  rosmarinic  acid  dominated  (30  –  50  mg/g  dry  leaves  extract).  Perilla  leaves  rich  in                polyphenols showed strong antioxidant and moderate antibacterial action.                                           Figure 1. Heat flow of MSSA (green) and MRSA (red) nontreated/treated by perilla water                                          extract determined by microcalorimetry.                 Acknowledgments: This work was supported by grants APVV-19-0056 and VEGA 1/0170/24.                                                                                                              79","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO5                   From koala snack to anti-microbial attack: Potency of 1,8-cineole against                                           drug resistant microorganisms                           Malwina Brożyna *, Bartłomiej Dudek , Milena Szymańska , Adam Junka     1                                          1,                                                               1                                                                                   1                     1 Wroclaw Medical University, Platform for Unique Models Application, Department of                                       Pharmaceutical Microbiology and Parasitology                                    *Corresponding author: malwina.brozyna@umw.edu.pl                 The escalating resistance of  microorganisms to antibiotics poses a formidable  challenge  to                global health, necessitating the exploration and validation of novel antimicrobial agents beyond                antibiotics. In this context, plant-derived compounds, notably essential oils, emerge as potent                alternatives due to their broad-spectrum antimicrobial properties. These oils, characterized by                their complex compositions of multiple constituents in varying proportions, may offer effective                strategies against antibiotic-resistant pathogens. Interactions among the constituents of essential                oils contribute to their overall antimicrobial activity, which may differ significantly from the                effects of their individual components.                Our  preceding  investigations  have  shown  that  eucalyptus  essential  oil,  predominantly                comprising 1,8-cineole, which accounts for 80% of its composition, substantially inhibits the                proliferation of a range of pathogens. This observation propelled a focused analysis on the                antimicrobial  activity  of  1,8-cineole  against  reference  strains  of  Staphylococcus  aureus,                Pseudomonas aeruginosa, and Candida albicans employing the microdilution method.                The study revealed that staphylococcal cells showed more than 90% reduction in 1,8-cineole                concentrations between 100 and 25 mg/ml. Complete inhibition of C. albicans was achieved at                a minimal concentration of 25 mg/ml, with a 50% reduction of cells at 1.6 mg/ml. However,                1,8-cineole exhibited no activity against P. aeruginosa within the tested concentration range.                The results underscore 1,8-cineole's potent inhibitory effect on the growth of S. aureus and                C. albicans,  highlighting  its  potential  as  a  promising  candidate  for  developing  treatments                targeting infections caused by these pathogens. Opting for a singular, well-defined component                such as 1,8-cineole rather than the complex essential oil might offer advantages in terms of                reduced  side  effects  and  circumventing  the  challenges  associated  with  essential  oil                standardization.  This  study  contributes  to  the  burgeoning  field  of  antimicrobial  research,                offering a foundation for further exploration of plant-derived compounds in the fight against                antibiotic resistance.                 This  research  was  funded  by  the  National  Science  Centre,  Poland  (Grant  No.                2021/41/N/NZ6/03305).                                                                                                                   80","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO6                    Using mass spectrometry for study of salicylic acid metabolism in plants                                                             1                                            1,                             Beata Budíková *, Jitka Široká , Asta Žukauskaitė , Ondřej Novák                                                                              2                                                                                             1                                              1  Laboratory of Growth Regulators                  Institute of Experimental Botany of the Czech Academy of Sciences \& Palacký University,                                       Šlechtitelů 27, 78371 Olomouc, Czech Republic                                             2  Department of Chemical Biology                  Faculty of Science, Palacký University, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic                                       *Corresponding author: beata.budikova@upol.cz                  Plant immunity is defined as the capacity of plants to prevent or ward off biological attacks by                pathogens. It involves the recognition of the pathogen by specific receptors and the triggering                of signalling pathways leading to processes that help plants to defend themselves. The defence                signalling is mediated by cross-communication of groups of plant hormones. Salicylic acid                (SA)  is  one  of  the  most  pronounced  plant  hormones  involved  in  control  of  immunity  and                defence mechanisms in plants. Defence related SA accumulation comes from its biosynthesis,                transport and possible release from some of its metabolites. The involvement of SA biosynthetic                pathways  as  well  as  extend  of  SA  metabolism  in  various  plant  species  are  still  under                investigation. Liquid chromatography tandem mass spectrometry (LC-MS/MS) is one of the                most commonly used analytical technics in plant hormone quantification. The selectivity and                high  sensitivity  enables  to  track  typically  low  concentrations  of  phytohormones.  The                comprehensive multiclass phytohormone LC-MS/MS profiling methods usually include only                SA (as the active compound) from the group of SAs. LC-MS/MS methods for determination of                defence  related  phytohormones  focus  on  SA,  or  some  on  its  biosynthetic  precursors  or                metabolites. In this study, we focus on identification of new SA metabolites and development                of liquid chromatography mass spectrometry methods for targeted, semi- or untargeted analysis                of biosynthetic precursors and metabolites of SA in plants.                  Acknowledgements: The authors thank  Palacký University (IGA_PrF_2024_013) and grant                (22-17435S) from the Czech Science Foundation (GAČR) for funding.                                                                                                                               81","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO7                   Quantification of Yang cycle intermediates and related compounds by LC-                                                   MS/MS in plants                                    Kateřina Cermanová *, Ondřej Novák , Michal Karady    1                                                                       1                                                      1,                 1  Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of                   Sciences, Faculty of Science of Palacký University, Olomouc CZ-78371, Czech Republic                                   *Corresponding author: katerina.cermanova01@upol.cz                   The amino acid methionine represents a fundamental compound in plant metabolism. Through                a set of methionine-recycling reactions, collectively called “Yang cycle”, and the compounds                arising  from  them,  it  influences  essentially  all  plant  processes.  Its  first  metabolite,                S-adenosylmethionine  (SAM)  represents  one  of  the  most  used  substrates  for  enzymatic                reactions in plants and mammals, providing the role of a methyl donor. In plants, SAM is used                for synthesis of 1-aminocyclopropane-1-carboxylic acid, which is converted to an indispensable                gaseous plant hormone ethylene, and serves also as a precursor in polyamine biosynthesis.                Accurate determination of the levels of Yang cycle metabolites is therefore very important in                understanding plant ontogenesis and various aspects of plant life, however verified profiling                methods are lacking. Quantification of these highly polar compounds presents a challenge due                to their low retention on reversed-phase columns, as well as the chemical instability and low                abundance of some of these metabolites. In this study, we have developed an UHPLC-MS/MS                method  for  simultaneous  determination  of  these  metabolic  intermediates  based  on  isotope                dilution. To improve retention and ionization efficiency, amino groups were derivatized and the                chromatographic  separation  was  achieved  using  a  C18  column  with  water  and  acetonitrile                containing formic acid as mobile phases. Multiple reaction monitoring mode was employed for                qualitative and quantitative detection of the target metabolites.                In addition to analysis of different plant biological samples, we have further used the method                to study the effect of exogenous methionine on endogenous levels of these compounds, as well                as the changes in metabolism upon stress treatments.                    Acknowledgements: The authors thank Palacký University for funding (IGA_PrF_2023_031)                and junior grant (20-25948Y) from the Czech Science Foundation (GAČR).                                                                                                                      82","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO8                      Functionalization of goat cheese with biomass and extracts from the                                   halophyte species Helychrisum picardii picardii                                                                                    2                                                                                                 1                                                         2                   Paula Carvalho , Maria João Rodrigues , Viana Castañeda-Loaiza , Isabel Ratão , Célia                                  1                                                        1                                                Quintas , Luísa Custódio *                                                                        2,                 1  MED, Mediterranean Institute for Agriculture, Environment and Development, Universidade                                do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal                    2  Centre of Marine Sciences (CCMAR), University of Algarve, Faculty of Sciences and                             Technology, Ed. 7, Campus of Gambelas, 8005-139, Faro, Portugal                                          *Corresponding author: lcustodio@ualg.pt                 In the food industry, additives play a vital role in extending the shelf life of products, facilitating                mass production, and enabling distribution across global markets. However, the use of synthetic                additives has been associated with an increased risk of health issues, including allergies and                cancers. There is a growing global trend towards healthier diets, favoring foods with natural                preservatives or ingredients. Commonly, such enhancements are applied to familiar foods like                cheese.  This  study  introduces  biomass  and  leaf  infusions  from  the  medicinal  halophyte                Helichrysum picardii, harvested in Southern Portugal, into goat cheese production both post-                and  pre-coagulation.  The  cheeses  were  assessed  for  microbial  safety,  physicochemical                properties, and antioxidant activity over 8 days at 4°C. The addition post-coagulation resulted                in a less yellow cheese, and the use of biomass led to a higher dry extract percentage than other                methods. The pH was lower when additions were made post-coagulation. Interestingly, the                introduction of plant material did not affect the cheese's antioxidant capacity. It did, however,                increase microorganism and fungal colony counts at 30°C, likely due to the plants' microbial                quality. Despite this, all cheese samples met legal standards for the examined pathogens. While                this  study  demonstrated  that  the  addition  of  plant  material  can  alter  the  physicochemical                characteristics of cheese without impacting its antioxidant properties, it also led to an increased                presence  of  microorganisms,  underscoring  the  importance  of  ensuring  the  microbiological                quality of the natural additives used.                 Acknowledgements: This work received Portuguese national funds from FCT - Foundation                for Science and Technology through projects UIDB/04326/2020                (https://doi.org/10.54499/UIDB/04326/2020), UIDP/04326/2020                (https://doi.org/10.54499/UIDP/04326/2020), LA/P/0101/2020                (https://doi.org/10.54499/LA/P/0101/2020), and PTDC/BAA-AGR/1391/2020. Viana                Castañeda-Loaiza acknowledges FCT for the PhD grant with the reference 2020.04541.BD.                M.J.R. was supported through the FCT program contract (UIDP/04326/2020) and L.C. by the                FCT Scientific Employment Stimulus (CEECIND/00425/2017).                                                                                                                83","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO9                     How ecotype influences the nutritional profile of the edible halophyte                             Sarcocornia perennis cultivated under saline conditions?                                                      1                                1,                                                                                                        3                  Luísa Custódio *, Eliana Fernandes , Maria João Rodrigues , Muki Shpigel , Moshe Sagi                                                                            1                                                                                           2                     1  Centre of Marine Sciences (CCMAR), University of Algarve, Faculty of Sciences and                             Technology, Ed. 7, Campus of Gambelas, 8005-139, Faro, Portugal                  2  Morris Kahn Marine Research Station, The Leon H. Charney School of Marine Sciences,                                         University of Haifa, Haifa 3498838, Israel                 3  The Jacob Blaustein Institutes for Desert Research, The Albert Katz Department of Dryland                                       Biotechnologies, Ben-Gurion University, Israel                                          *Corresponding author: lcustodio@ualg.pt                 Halophytes,  plants  thriving  in  saline  conditions,  are  known  for  their  rich  nutritional  and                bioactive profiles, offering numerous health benefits. The cultivation of halophytes presents a                strategic solution to combat the effects of climate change, particularly addressing issues such                as freshwater scarcity and soil and water salinization. These plants can be used directly as fresh                vegetables with unique flavors or processed into functional food additives that confer health                benefits. Sarcocornia A.J. Scott is a perennial halophyte suited to harsh saline environments                and is valued in gourmet cooking for its unique flavor and crunchy texture. This study evaluated                how different Sarcocornia perennis ecotypes affect the plant's nutritional content when grown                under greenhouse conditions with saline irrigation. Two ecotypes were chosen based on their                distinct habitats and growth traits. Cuttings taken in January 2021 in Ria Formosa (Algarve,                Portugal) were rooted and potted in a coco peat and perlite mix and irrigated with brackish well                water. After 30 days, plants were harvested at a marketable size of 10-15 cm and evaluated for                proximate  composition  and  minerals  contents.  Both  ecotypes  showed  similar  proximate                compositions; however, mineral content varied, with Sp.E1 showing higher Na (103.06 mg/g                DW), Mn (85.23 µg/g DW), and Zn (30.06 µg/g DW). In contrast, Sp.E2 was richer in K (21.89                µg/g DW) and Pb (3.12 µg/g DW). These findings imply that selecting the right Sarcocornia                ecotype is crucial for optimizing the nutritional value of the biomass for saline agriculture.                 Acknowledgements: This work received Portuguese national funds from FCT - Foundation                for Science and Technology through projects UIDB/04326/2020                (https://doi.org/10.54499/UIDB/04326/2020), UIDP/04326/2020                (https://doi.org/10.54499/UIDP/04326/2020), LA/P/0101/2020                (https://doi.org/10.54499/LA/P/0101/2020), PT-IL/0003/2019), and PTDC/BAA-                AGR/1391/2020. Viana Castañeda-Loaiza acknowledges FCT for the PhD grant                2020.04541.BD. M.J.R. was supported through the FCT program contract                (UIDP/04326/2020) and L.C. by the FCT Scientific Employment Stimulus                (CEECIND/00425/2017).                                                                                                               84","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO10                   Comparison of antibiofilm efficacy of selected azole antifungals and plant                                  essential components in Candida albicans strains                                      Eva Čonková *, Peter Váczi , Miriam Bačkorová                                                                                     2                                                  1,                                                                 1                     1 Department of Pharmacology and Toxicology, University of Veterinary Medicine and                                         Pharmacy, 041 81, Košice, Slovak Republic                    2 Department of Pharmaceutical Technology, Pharmacognosy and Botany, University of                            Veterinary Medicine and Pharmacy, 041 81, Košice, Slovak Republic                                        *Corresponding author: eva.conkova@uvlf.sk                 The ability to form a biofilm is one of the important factors of virulence in C. albicans, which                usually leads to the emergence of resistance to commonly used antifungal agents in clinical                        1,2                practice . The present study is aimed at comparing the antibiofilm effectiveness of three azole                antifungals (miconazole, itraconazole, clotrimazole) and three components of plant essential                oils (eugenol, cinamaldehyde, carvacrol). For the antifungals tested, the planktonic cells of                C. albicans showed the highest susceptibility (30 strains, 100%) to miconazole (MIC 0.03-0.06                µg/mL) and itraconazole (MIC 0.03 µg/mL), followed by clotrimazole (MIC 0.03-1 µg/mL, 29                strains, 96.7%). Regarding the tested plant essential oil components, the best antifungal efficacy                was found with carvacrol (MIC 50 µg/mL), followed by cinnamaldehyde (MIC 50-200 µg/mL)                and eugenol (MIC 200-800 µg/mL). All three tested antifungals (miconazole, itraconazole and                clotrimazole) were ineffective against adhered cells (0 h biofilm) and mature biofilm (48 h                biofilm) of C. albicans. Out of the three plant essential oil components tested, carvacrol reached                the highest activity against adhered cells (MIC 100 µg/mL), then cinnamaldehyde (MIC 100-                400 µg/mL) and eugenol (MIC 400-800 µg/mL). In mature biofilm, the best antibiofilm activity                was  observed  for  carvacrol  and  cinnamaldehyde,  both  MIC  400-800  µg/mL,  followed  by                eugenol (MIC 800 µg/mL). The obtained results indicate that the components of plant essential                oils could play an important role in the fight against biofilm formation in C. albicans.                 Acknowledgements: Authors thank to  Ministry of Education KEGA, Grant No. 003UVLF-                4/2024,  Medicinal  mushrooms  in  pharmacy  and  building  3D  collections  of  medicinal                mushrooms                 References:                1.     Angiolella, L., Rojas, F., Giammarino, A., Bellucci, N., Giusiano, G. Identification of virulence factors in                isolates  of Candida  haemulonii, Candida  albicans and Clavispora  lusitaniae with  low  susceptibility  and                resistance   to     fluconazole    and     amphotericin   B. Microorganisms, 2024, 12,   212.                https://doi.org/10.3390/microorganisms12010212                2.     Massey,  J.,  Zarnowski,  R., Andes, D.  Role  of  the  extracellular  matrix  in Candida biofilm  antifungal                resistance, Fems Microbiol. Rev., 2023, 47, 6, 1–6. https://doi.org/10.1093/femsre/fuad059                                                                                                                85","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO11                   Study of antimicrobial activity of Sorbus domestica L. leaf extract against                                                Staphylococcus aureus                 Kamila Dokupilová *, Ema Kostovčíková , Eva Drobná , Elena Kurin , Pavel Mučaji , Silvia                                   1,                                                                                    1                                                                                                   1                                                         1                                                                       2                                                                    1                                                      Bittner Fialová                    1  Comenius University Bratislava, Department of Pharmacognosy and Botany, Faculty of                                      Pharmacy, Odbojárov 10, SK – 832 32 Bratislava                    2  Comenius University Bratislava, Department of Cell and Molecular Biology of Drugs                                Faculty of Pharmacy, Odbojárov 10, SK – 832 32 Bratislava                                       *Corresponding author: dokupilova6@uniba.sk                 Sorbus domestica L. (Rosaceae) is a tree cultivated in vineyards and gardens for its apple or                pear-shaped fruits. Nowadays, it is almost forgotten, and its therapeutic potential is still not                efficiently described. This study aimed to explore the antimicrobial properties of the leaf water                extract of S. domestica against methicillin-susceptible Staphylococcus aureus (MSSA). The                minimal inhibitory (MIC) and minimal bactericidal (MBC) concentrations were determined by                the  conventional  broth-microdilution  method  (BMD). The  antibacterial  mechanism  against                MSSA was studied via a new approach of isothermal microcalorimetry (IMC) measuring the                metabolic  activity  of  bacteria.  Phytochemical  analysis  was  performed  to  determine  the                polyphenolic compounds of the extract. Broth-microdilution method showed MIC 10,0 mg/ml                and MBC over 20,0 mg/ml. By the IMC, the antimicrobial activity of the extract, shown in the                graphical part of this abstract, was evident. All the measured concentrations (10,0 – 1,25 mg/ml)                delayed the time to peak and decreased the total heat, compared to growth control, from which                the concentration 10,0 mg/ml showed the most significant effect (Fig. 1). These results showed,                that the leaf extract of S. domestica has some antibacterial potential against MSSA evaluated                by  BMD  and  IMC  methods.  Moreover,  with  the  IMC  method,  it  was  possible  to  notice                antimicrobial activity in 8 times lower concentrations than BMD.                                  Figure 1 The influence of S. domestica leaf extract on growing curves of MSSA expressed as                                                     heat flow in time.                  Acknowledgements: Authors thank Grants: UK/3037/2024, VEGA 1/0170/24, APVV-19-0056.                                                                                                              86","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO12                       Chromatographic separation of xanthones from Maclura pomifera                                   1,                                                     1                Margita Dvorská *, Ivana Machalová , Jan Chmura , Milan Malaník , Marie Čulenová , Emil                                                                  1                                                                                   1                                                                                                    1                                                                  1                                                        Švajdlenka                           1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University,                                     Palackého tř. 1946/1, 612 00 Brno, Czech Republic                                      *Corresponding author: dvorskam@pharm.muni.cz                 Research  on  biologically  active  substances  focuses,  among  other  things,  on  monitoring                biological activity as a function of the chemical structure of the tested compounds. Substitution                of the basic skeleton and its modification often play a major role.                Xanthones are a remarkable group of natural secondary metabolites occurring in only a few                families and having  interesting pharmacological  effects. For example,  in  vitro  cytotoxic or                antitumor  activity  [1],  cholinesterase  inhibition  [2]  or  antioxidant  effect  [3]  have  been                described. The stem and root bark of Maclura pomifera (Raf.) C.K. Schneid. (Moraceae) was                selected as a suitable source for their isolation. The root bark was extracted with methanol and                subsequently separated. Ethanolic extract of stem bark was firstly fractionated by liquid/liquid                extraction  and  n-hexane,  chloroform,  and  ethyl  acetate  fractions  were  obtained.  Individual                extracts and subsequently selected fractions were separated by chromatographic methods.                Column chromatography, preparative TLC, and semipreparative HPLC led to the isolation of                ten xanthones (prenylated and unprenylated), two of them were new compounds. TLC, HPLC,                MS, and 1D and 2D NMR experiments were used to identify these compounds. The highest                yields were for toxyloxanthone C and osajaxanthone from stem bark, and macluraxanthone                from root bark.                The isolated xanthones will be subjected to biological activity screening mainly focusing on                anti-inflammatory, antioxidant or cytotoxic effects for future potential therapeutic applications.                Due to the isolation of compounds with differently modified substituents, their set will also help                to obtain information on the structure-activity relationship.                 Acknowledgements:  GAČR  project  GA23-04655S,  Role  of  prenylation  and  glycosylation                patterns in anti-inflammatory activity and metabolism of natural phenolic compounds                 References:                1.     da Costa, C.T. Comparison of methods for extraction of flavanones and xanthones from the root bark of                the   osage   orange   tree   using   liquid   chromatography. J.   Chromatogr.  A. 1999, 831,   167-178.                https://doi.org/10.1016/S0021-9673(98)00937-6                2.     Khan, M.T.H. Cholinesterase inhibitory activities of some flavonoid derivatives and chosen xanthone and                their    molecular    docking    studies.   Chem.     Biol.    Interact. 2009, 181,   383-389.                https://doi.org/10.1016/j.cbi.2009.06.024                3.     Luo, C.-T. Antioxidant xanthones from Swertia mussotii, a high altitude plant. Fitoterapia. 2013, 91, 140-                147. https://doi.org/10.1016/j.fitote.2013.08.021                                                                                                                87","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO13                 Saccharides in Polygoni cuspidati rhizoma et radix decoction as biologically                    active compounds stimulating the proliferation of gingival fibroblasts                                                           2                                                                              3                                       1,4,                        Magdalena Fast *, Jakub Hadzik , Anna Choromańska , Adam Matkowski        1,                                                          4                                        Bożena Karolewicz , Izabela Nawrot-Hadzik  1                  1  Department of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical                                              University, 50-556 Wroclaw, Poland                                  2  Department of Dental Surgery, Wroclaw Medical University,                 3  Department of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University,                                                   50-556 Wroclaw, Poland                   4  Department of Drug Form Technology, Wroclaw Medical University, 50-556 Wroclaw, Poland                                       *Corresponding author: magdalena.fast@.umw.edu.pl                Rhizomes of Polygonum cuspidatum were used in Traditional Chinese Medicine (TCM) for                centuries. Currently, it is considered as pharmacopoeial raw material in Europe and China.                Scientific literature indicates that this raw material is a rich source of compounds with anti-                inflammatory  and  antioxidant  activity.  Latest  study  (1)  of  our  research  team  showed  that                extracts  from  rhizomes  of  Polygonum  cuspidatum,  especially  the  water  decoction  possess                wound  healing  stimulating  activity.  Decoction  significantly  stimulated  human  gingival                fibroblasts  to  proliferate,  migrate,  and  increase  the  synthesis  of  collagen  III. These  results                confirm the traditional use of a decoction of this raw material in the treatment of inflammation                and wounds mouth. The aim of this research project was to test the hypothesis that saccharides                were  responsible  for  the  observed  high  activity  of  the  decoction.  For  this  purpose  water                decoctions were prepared from the raw material. A gradient precipitation of saccharides from                aqueous solutions was performed using appropriate concentrations of ethanol. The decoctions,                different fractions of saccharides and the residue after isolation of saccharides were subjected                to in vitro MTT assays on human gingival fibroblasts. All the above samples were subjected to                phytochemical  analysis  using  FTIR  (Fourier  Transform  Infrared  Spectroscopy),                thermogravimetry, HPLC-MS (HPLC/DAD/ESI-HR-QTOF-MS method) and HPLC with RI                refractometric  detector  for  further  separation  and  identification.  The  highest  increase  in                fibroblast viability was observed for the oligosaccharide fraction, but the decoction and other                fractions  also  showed  statistically  significant  activity.  Phytochemical  studies  confirm  the                abundance of saccharides in the most active fraction. The isolation of saccharides with a studied                chemical structure that exhibit wound-healing stimulating properties provides opportunities for                their use in regenerative dentistry. Our reports confirm the validity of the traditional use of                decoctions as oral rinses and identify saccharides as an important group of compounds with                significant biological activity.                 Acknowledgements:  The  study  was  supported  by  grants:  National  Science  Centre,  Poland                2023/07/X/NZ7/00166 and SUBK.D190.24.076                References:                1.     Hadzik, J.; Choromańska, A.; Karolewicz, B.; Matkowski, A.; Dominiak, M.; Złocińska, A.; Nawrot-                Hadzik, I. Oral Wound Healing Potential of Polygoni Cuspidati Rhizoma et Radix Decoction—In Vitro Study.                Pharmaceuticals 2023, 16, 267. https://doi.org/10.3390/ph16020267                                                                                                               88","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO14                       Susceptibility of intestinal bacteria involved in colorectal cancer                 pathogenesis to extracts from Philippine medicinal plant species and plant-                                                   derived compounds                          Barbora Fiserova*, Tomas Kudera, Marketa Houdkova, Ladislav Kokoska                  Laboratory of Ethnobotany and Ethnopharmacology, Department of Crop Science and Agroforestry,                  Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 00                                               Praha – Suchdol, Czech Republic                                           *Corresponding author: fiserovab@ftz.czu.cz                Colorectal cancer (CRC) is the second most deadly cancer with 0.9 million deaths worldwide                        1                in 2020 , with China and  the US having the  highest estimated number of new cases . Gut                                                                                                     2                dysbiosis is one of the factors associated with an increased risk of developing CRC. Various                phytochemicals  and  their  synthetic  analogs  (e.g.,  quinoline  derivates),  together  with                                                                                                3                traditionally used herbs, have been found to inhibit gut pathogenic microorganisms ; however,                their effect on CRC associated microorganisms has not been determined yet. Therefore, we                tested in vitro growth-inhibitory effects of 12 compounds (berberine, bismuth subsalicylate,                carbadox,  ferron,  8-hydroxyquinoline,  chloroxine,  nitroxoline,  olaquindox,  salicylic  acid,                sanguinarine, tannic acid, and zinc pyrithione), and 10 ethanol extracts obtained from medicinal                plant  species  traditionally  used  in  Philippines  to  cure  gastrointestinal  disorders  (Acalypha                grandis,  Artocarpus  blancoi,  A.  camansi,  A.  elasticus,  A.  lakoocha,  Citrus  microcarpa,                Crescentia cujete, Dysoxylum blancoi, Euphorbia thymifolia, and Merremia peltata), and six                antibiotics  against  CRC-causing  pathogens  (Bacteroides  fragilis,  Clostridium  septicum,                Esherichia coli, F. necrophorum, F. nucleatum, P. anaerobius, and Streptococcus bovis) using                broth-microdilution assay. In this study, all  compounds and plant  extracts shown a  certain                degree of antimicrobial effect with minimum inhibitory concentrations (MICs) ranging from                0.5-512  µg/ml.  Carbadox  (MICs  =  0.5-32  μg/mL),  nitroxoline  (MICs  =  8-16  μg/ml),  zinc                pyrithione (MICs = 4-32 μg/ml) and chloroxine (MICs = 4-64 μg/ml) were the most active.                Plant extract of A. lakoocha bark (256 µg/ml) inhibited growth of E. coli, A. elasticus leaves                (512 µg/ml) inhibited growth of S. bovis. Leaves extract of A. blancoi, A. elasticus (256 µg/ml)                and D. blancoi (bark) (512 µg/ml) significantly inhibited bacterial growth of S. bovis. E. coli,                C. septicum,  and S. bovis were the most susceptible bacteria with MICs ≥ 4 μg/ml. These                findings indicate that studied chemicals and plant-extracts have potential to inhibit growth of                microorganisms associated with CRC development.                Acknowledgement: This research was financially supported by the Czech University of Life                Sciences Prague (projects IGA 20233109) and Foundation \"Nadání Josefa, Marie a Zdeňky                Hlávkových\".                 References:                1.     World Health Organization. Colorectal Cancer Awareness Month 2021                Available online: https://www.iarc.who.int/featured-news/ccam2021/ (accessed on 11 May 2022)                2.     Yue, X., Pengfei, X. Global colorectal cancer burden in 2020 and projections to 2040. Transl. Oncol.                2021, 14 (10), 101174. https://doi.org/10.1016/j.tranon.2021.101174                3.     Kudera, T. et al. In vitro selective growth-inhibitory activities of phytochemicals, synthetic phytochemical                analogs,  and  antibiotics  against  diarrheagenic/probiotic  bacteria  and  cancer/normal  intestinal  cells.                Pharmaceuticals. 2020, 13 (9), 233. https://doi.org/10.3390/ph13090233                                                                                                              89","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO15                        Antiproliferative diterpenes from three Plectranthus s.l. species                                                                              3                                                                                               3                                                       2                                    1,                    Mária Gáborová *, Máté Vágvölgyi , Bizhar Ahmed Tayeb , Renáta Minorics , István                   Zupkó , Ondřej Jurček , Szabolcs Béni , Renata Kubínová , György Tibor Balogh , and                                                                                                   6                         3                                                                            1                                                         5                                         1,4                                                      Attila Hunyadi 2                    1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, 612 00 Brno,                                                          Czechia                   2  Institute of Pharmacognosy, Faculty of Pharmacy, University of Szeged, 6720 Szeged,                                                         Hungary                   3  Institute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of                                               Szeged, 6720 Szeged, Hungary                  4  Department of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czechia                5  Department of Analytical Chemistry, Institute of Chemistry, Eötvös Loránd University, 1117                                                    Budapest, Hungary                6  Department of Pharmaceutical Chemistry, Semmelweis University, 1092 Budapest, Hungary                                     *Corresponding author: gaborovam@pharm.muni.cz                 The genus Plectranthus s.l. (Lamiaceae) has been used in traditional medicine for centuries,                particularly in the treatment of various digestive, respiratory, urogenital, and dermatological                disorders. Phytochemical studies revealed a production of phenolic compounds and terpenes as                major secondary metabolites. Among them, structurally diverse, highly oxygenated diterpenes                are  the  most  prominent  group  which  gathered  considerable  scientific  interest  due  to  their                promising biological effects, especially antimicrobial, antiprotozoal, and cytotoxic activities.                Abietane diterpenes from subclasses of royleanones, hydroquinones and quinone methides were                shown to be the most potent antiproliferative and/or cytotoxic agents.                In our current study, we focused on exploring the methanol extracts derived from the aerial                parts of C. comosus, C. forsteri 'Marginatus', and P. ciliatus. We isolated fourteen diterpenes                from these extracts, which belong to the abietane, ent-clerodane, and ent-kaurane classes. Three                of them, including 3-O-acetylornatin G, 3,12-di-O-acetylornatin G, and ornatin B methyl ester,                were identified as new natural products. The isolated compounds were examined in silico for                their  physicochemical  and  early  ADME  parameters.  Their  antiproliferative  effects  were                investigated in vitro using human breast (MDA-MB-231 and MCF-7), cervical (HeLa), and                glioblastoma  (U-87  MG)  cancer  cell  lines.  The  royleanone  and  hydroquinone  abietanes                exhibited  the  most  potent  antiproliferative  activity  against  all  cancer  cell  lines  tested,                particularly against glioblastoma cells, with IC50 values ranging from 1.1 to 15.6 μM.                 Acknowledgements: Authors thank to the GACR (GA23-04655S), the NKFIH, Hungary (K-                134704), and the ITM, Hungary (TKP2021-EGA-32), and Josef Dadok National NMR Centre                of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2023042) and European Regional                Development  Fund-Project  „UP  CIISB“  (No.  CZ.02.1.01/0.0/0.0/18_046/0015974).  M.                Vágvölgyi was supported by the KIM, Hungary (ÚNKP-23-4-222).                                                                                                              90","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO16                      p-Topolin and its mesylate as possible active components of skin care                                                         products                                                             1                                                                            1                            1,                                                                                               1                  Jiří Hanyk *, Alena Rajnochová Svobodová , Alena Ryšavá , Daniela Horáková , Bohumil                                                                                         1                                                       3                                         2                                                                        3                                  Zálešák , Dardan Klos , Lucie Plíhalová , Jitka Vostálová                    1  Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry,                            Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic                 2  Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, I. P. Pavlova 6,                                             775 20 Olomouc, Czech Republic                3  Department of Chemical Biology, Faculty of Science, Palacký University, Šlechtitelů 27, 783                                                71, Olomouc, Czech Repubic                                         *Corresponding author: jiri.hanyk@upol.cz                 Purine-based  phytohormones  are  involved  in  the  regulation  of  many  processes  in  plants,                including senescence and are therefore of interest in skin care. The best known is kinetin (KIN;                  6                N -furfurylaminopurine) that has antioxidant, cell regenerative and anti-ageing properties and                                                             1                is already used in dermatological preparations . Structurally close to KIN is para-topolin (pT;                6-(4-hydroxybenzylamino)purine). Recently, pT has been shown to have beneficial effects on                skin structure, wrinkles, roughness and non-inflammatory acne lesions . pT use in skin care is                                                                                    2                limited due to its poor water solubility. Therefore, a much more water soluble mesylate salt of                pT (pTM) was prepared.                In this study we compared biological activity of pTM with the parent substance pT. KIN was                used  as  a  standard  component  used  in  practice.  The  basic  safety  characteristics  for  using                substances  in  dermatological  preparations,  particularly  (photo)stability  and  (photo)toxicity                were evaluated. Further effect on the signalling pathway controlled by Nrf2 transcription factor                that modulates cell  antioxidant  capacity, and UVA-photoprotective properties were studied.                Results will be discussed at the poster presentation.                 Acknowledgements: This work was financially supported by the grant IGA_LF_2024_011 and                Institutional Support of Palacký University Olomouc (RVO 61989592).                 References:                1.     Kadlecová, A.; Maková, B.; Artal-Sanz, M.; Strnad, M.; Voller, J. The plant hormone kinetin in disease                therapy and healthy aging. Ageing Res Rev. 2019, 55:100958. doi: 10.1016/j.arr.2019.100958.                2.     Garcia, R.L.; McCullough, J.L.; Reece, B.; Massino, F.J.  Novel Topical Cytokinin Improves Coarse                Wrinkles and Skin Roughness Without Skin Irritation. J Drugs Dermatol. 2018, 17(7): 772-779. PMID: 30005100                                                                                                                       91","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO17                      Iron and copper chelation by benzoic acids and their effect on metal                                      catalyzed Fenton reaction and hemolysis                                  Patrícia Harčárová*, Zuzana Lomozová, Přemysl Mladěnka                         Charles University, Faculty of Pharmacy in Hradec Králové, Czech Republic                                        *Corresponding author: harcarop@faf.cuni.cz                 Flavonoids  are  secondary  plant  metabolites  that  are  a  common  part  of  our  diet  and  their                consumption has positive health effects at least according to epidemiological studies. However,                flavonoid can have both antioxidant and prooxidant effects . The biological relevance of such                                                                         1,2                effects  is  disputable,  as  flavonoids  are  massively  metabolized  into  small  phenolics  by                microbiota before reaching systemic circulation. One class of these metabolites are benzoic                acids. It is possible that the biological properties attributed to flavonoids are, in fact, caused by                                3                their metabolites .                This analysis included investigation of the interactions between benzoic acids and transition                metals iron and copper. The chelating activity of benzoic acids was pH dependent. From the                tested substances, only 3,4-dihydroxybenzoic acid demonstrated a strong capacity for chelating                iron ions. The same compound also demonstrated the capability to reduce ferric and cupric ions.                The  screening  of  copper  chelation  activity  with  hematoxylin  method  showed  that  3,4-                dihydroxybenzoic acid demonstrated the highest copper-chelating activity, again. Subsequently,                the determination of the effect on copper-induced lysis of rat red blood cells was used to verify                the antioxidant or pro-oxidative action of the tested compounds. None of the tested compounds                exhibited  the  ability  to  protect  rat  erythrocytes  from  copper  toxicity.  Only  2,4-                dihydroxybenzoicacid and 3,4-dihydroxybenzoic acid were able to decrease the production of                hydroxyl radicals arising during the metal-catalyzed Fenton reaction at pH 4.5 and 7.5. These                results  showed  significant  differences  in  the  anti-  and  prooxidant  activities  of  the  tested                substances based on experimental conditions.                 Acknowledgements: Authors thank to the Charles University project Cooperatio.                 References:                1.  Del Rio D., Rodriguez-Mateos A., Spencer J. P., Tognolini M., Borges G., Crozier A. Dietary (poly)phenolics                    in  human  health:  structures,  bioavailability,  and  evidence  of  protective  effects  against  chronic  diseases.                    Antioxid. Redox Signal. 2013, 18, 1818. https://doi.org/10.1089/ars.2012.4581                2.  Panche  A.  N.,  Diwan  A.  D.,  Chandra  S.  R.  Flavonoids:  an  overview.  J.  Nutr.  Sci.  2016,  5,  e47.                    https://doi.org/10.1017/jns.2016.41                3.  Kim D. H., Jung E. A., Sohng I. S., Han J. A., Kim T. H., Han M. J. Intestinal bacterial metabolism of                    flavonoids  and  its  relation  to  some  biological  activities.  Arch.  Pharm.  Res.  1998,  21,  17.                    https://doi.org/10.1007/BF03216747                                                                                                                   92","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO18                  Evaluating of anti-quorum sensing activity of natural phenolic compounds                                                                              1                                                                                             1                             Martin Helcman *, Jakub Treml , Karel Šmejkal , Marie Čulenová                                                            2                                             1,                                               1  Department of Natural Drugs,                  2  Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University,                                     Palackého tř. 1946/1, 612 00 Brno, Czech Republic                                     *Corresponding author: helcmanm@pharm.muni.cz                 Inhibition of quorum sensing, bacterial cell-to-cell communication, represents a very attractive                target  for  novel  antibacterial  compounds.  Using  a  light-producing  biosensor  strain  Vibrio                harveyi MM30, we tested 16 natural compounds: 7 cannabinoids, 7 arylbenzofurans and 2                flavonoids for anti-quorum sensing activity. There are two main genetic circuits responsible for                the  regulation  of  quorum  sensing  behaviour  in  V.  harveyi: AI-1  and AI-2.  While AI-1  is                regulated by strain-specific molecules, AI-2 production is common among various species of                bacteria. The mutant strain V. harveyi MM30 has knockout genes for production of AI-2 but is                still able to react to exogenous AI-2. Therefore, we added AI-2 containing medium to its culture                and investigated the direct activity of subinhibitory concentrations of our substances on the AI-                2 receptor. For comparison, we measured the activity of the same compounds, but without                adding AI-2, so we obtained the results also for the AI-1 inhibitory activity. According to the                results of this experiment, we selected 3 compounds and added them at various subinhibitory                concentrations to the culture of methicillin-resistant Staphylococcus aureus (MRSA). After the                spent media was added to the culture of V. harveyi MM30, we obtained the information on the                activity of these compounds on AI-2 production in the MRSA strain.                                                                                                                                             93","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO19                   Auxin metabolism: unravelling novel pathways and metabolites by liquid                                   chromatography-mass spectrometry analysis                                                                                                      1                                                                                     2                             1                                                1                                                                   2                 Pavel Hladík , Federica Brunoni , Asta Žukauskaitė , Marek Zatloukal , Ondřej Novák , and                                                                  1,                                                       Aleš Pěnčík *                  1 Laboratory of Growth Regulators, Institute of Experimental Botany, The Czech Academy of                       Sciences \& Faculty of Science, Palacký University - Olomouc (Czech Republic)                        2 Department of Chemical Biology and Genetics, Centre of the Region Haná for                    Biotechnological and Agricultural Research, Faculty of Science, Palacký University -                                                 Olomouc (Czech Republic)                                         *Corresponding author: ales.pencik@upol.cz                 Proper  plant  growth  and  development  are  intricately  regulated  by  a  group  of  bioactive                compounds known as plant hormones (phytohormones). These substances function optimally                within a narrow concentration range, necessitating strict regulation of their levels in plant cells                and organs. This regulation primarily occurs through biosynthesis, metabolism and transport                mechanisms. Among the diverse groups of phytohormones, auxins were the first to be identified                owing to their profound effects on plant tropisms.  Two essential endogenous auxins are indole-                3-acetic acid (IAA) and phenylacetic acid (PAA). These compounds exhibit biological activity                only in their free, unconjugated form, as enzymatic reactions produce metabolites that serve as                temporary storage and transport forms, as well as degradation products. This conversion occurs                either through irreversible oxidation or reversible conjugation with amino acids and sugars. So                far, the metabolism of PAA and IAA appears quite similar, as the same enzymes catalyse the                synthesis of identical conjugates.                In  recent  years,  many  new  discoveries  have  been  made  in  the  field  of  auxin  metabolism,                expanding our knowledge about conjugates and catalysing enzymes. However, most of this                research is connected to IAA and Arabidopsis thaliana as a model plant. Therefore, we aim to                broaden the understanding of metabolism of two endogenous auxins (IAA and PAA) across                various land plant species and their organs. By employing liquid chromatography coupled with                tandem  mass  spectrometry  (LC-MS/MS)  we  identified  novel  IAA  and  PAA  endogenous                metabolites. Following experiments involved complex IAA and PAA metabolite profiling in                various land plant species and their organs and elucidation of enzymes responsible for these                reactions utilizing bacterial enzymatic assays.                 References:                1.     Hladík P., Petřík I., Žukauskaitė A., Novák O., Pěnčík A. (2023) Metabolic profiles of 2-oxindole-3-                acetyl-amino acid conjugates differ in various plant species. Front. Plant Sci. 14, 1217421.                2.     Brunoni F., Pěnčík A., Žukauskaitė A., Ament A., Kopečná M., Collani S., Kopečný D., Novák O. (2023)                Amino acid conjugation of oxIAA is a secondary metabolic regulation involved in auxin homeostasis. New Phytol.                238, 2264–2270.                                                                                                                 94","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO20                                  Yeast glucan particles – bioactive drug vehicles                                                                   1,                                                       Jan Hošek *                  1  Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Palackého                                        tř. 1946/1, CZ-612 00 Brno, Czech Republic                                       *Corresponding author: hosekj@pharm.muni.cz                 Yeast  glucan  particles  (YGPs)  are  promising  agents  for  the  delivery  of  biologically  active                compounds as drugs. YGPs possess their own biological activities and can act synergistically                with their cargo. β-Glucans, the main component of yeast cell walls, can modulate both innate                and adaptive immune responses via their recognition by innate immune cells and activated                immune cells then promote immune responses.                Glucan particles protect payloads from the harsh conditions in the gastrointestinal tract and can                interact with the intestinal immune system,  they  might increase the bioavailability of  their                cargo.  Immune  cells  associated  with  Peyer’s  patches  in  small  intestine  can  then  use  the                lymphatic  system  to  distribute  the  cargo  of  the  YGPs  to  various  organs  of  the                reticulo-endothelial  system,  such  as  the  liver,  the  lung,  the  spleen,  and  the  kidney,  or  into                inflamed sites and tumours. These features make YGPs suitable candidates for the oral delivery                of diagnostic or therapeutic compounds.                 Acknowledgements: Authors thank to the Czech health research council of the Ministry of                Health  of  the  Czech  Republic,  Grant  No.  16-  27522A,  Natural  bioactive  compounds                encapsulated in glucan microparticles as biomaterials suitable in the treatment of inflammatory                bowel diseases.                                                                                                                                              95","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO21                 Liquid matrix volatilization methods for susceptibility testing of respiratory                               bacteria to volatile agents in liquid and vapour phase                                  Marketa Houdkova*, Aishwarya Chaure, Ladislav Kokoska                    Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech                        University of Life Sciences Prague, 165 00 Prague - Suchdol, Czech Republic                                        *Corresponding author: houdkovam@ftz.czu.cz                 Respiratory infections belong to the leading causes of morbidity and mortality throughout the                world. Inhalation therapy is possible way of treatment when the active agents are delivered                                                                 1                directly to the site of infection in respiratory system . Plant volatile agents such as essential oils                (EOs) and their constituents are of great potential for inhalation due to their volatility. However,                lack of knowledge on antimicrobial activity of their vapours is a main obstacle for their practical                use. Due to the high volatility and hydrophobicity of plant volatiles, conventional laboratory                methods of antimicrobial susceptibility testing face specific problems. Recently, broth micro-                and  macro-dilution  volatilization  assays  have  been  developed  in  our  laboratory  for  the                evaluation of the antimicrobial potential of volatiles in liquid and vapour phase . With aim to                                                                                            2,3                validate an appropriateness of both methods, the antibacterial activity of plant-derived volatiles                namely  8-hydroxyquinoline,  β-thujaplicin,  thymohydroquinone,  thymol,  thymoquinone  and                EOs obtained from Alpinia oxymitra pericarp, A. elegens leaf, Cymbopogon citratus aerial part,                and  Trachyspermum  ammi  seed  was  evaluated  against  respiratory  bacterial  pathogens                Haemophilus influenzae, Staphylococcus aureus, and Streptococcus pneumoniae. As a result,                all volatiles tested have shown a certain degree of antibacterial effect with minimum inhibitory                concentrations  (MICs)  ranging  2–512  and  8–1,024  µg/mL  in  liquid  and  vapour  phase,                respectively. 8-hydroxyquinoline possessed the highest antibacterial potential against S. aureus                in the liquid phase (MIC = 2 µg/mL). Vapours of 8-hydroxyquinoline and thymoquinone were                the most active against S. aureus and H. influenzae, respectively (both MIC = 8 µg/mL).                In summary, presented assays are suitable for simple and rapid susceptibility testing of bacterial                pathogens to volatiles in the liquid and vapour phase. They allow a cost- and labour-effective                high-throughput  screening  using  commercially  available  microtubes  or  microplates.  Both                methods have been validated for a development of applications for treatment of respiratory                infections based on volatile antimicrobials.                 Acknowledgements: This research was financially supported by the Czech University of Life                Sciences Prague [project IGA 20233109].                 References:                1.     Houdkova, M; Kokosko, L. Volatile antimicrobial agents and in vitro methods for evaluating their activity                in the vapour phase: A review. Planta Med. 2020, 86(12), 822-857. https://doi.org/10.1055/a-1158-4529                2.     Houdkova, M.; Rondevaldova, J.; Doskocil, I.; Kokoska, L. Evaluation of antibacterial potential and                toxicity of plant volatile compounds using new broth microdilution volatilization method and modified MTT                assay. Fitoterapia. 2017, 118, 56-62. https://doi.org/10.1016/j.fitote.2017.02.008                3.     Houdkova, M.; Chaure, A.; Doskocil, I.; Havlik, J.; Kokoska, L. New broth macrodilution volatilization                method for antibacterial susceptibility testing of volatile agents and evaluation of their toxicity using modified                MTT assay in vitro. Molecules. 2021, 26, 3179. https://doi.org/10.3390/molecules26144179                                                                                                              96","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO22                      Bioactive 3D supramolecular coordination architectures of bile acids                                                                                         5                                                                         5                                                             4,5                                                                                                      2,3                  Subhasis Chattopadhyay  1,2,3, *, Robin Durník , Jan Kuta , Pavel Babica , Radek Marek ,                                                     Ondřej Jurček 1,2,3                 1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého 1946/1,                                                61200 Brno, Czech Republic                2  Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno,                                                      Czech Republic                   3  CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5,                                                62500 Brno, Czech Republic                  4  Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500                                                   Brno, Czech Republic                    5  RECETOX, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czech                                                         Republic                                    *Corresponding author: schattopadhyay@mail.muni.cz                 Natural  chiral  hydrophobic  cavity/pocket  containing  structures  are  important  for  many                biological  functions  (e.g.,  catalytic  metalloenzymes  or  transport  proteins). To  mimic  these                natural systems and mechanisms, development of artificial supramolecular systems (e.g., cages                or macrocycles) from chiral natural molecules is desirable. Coordination-driven self-assembly                is  a  well-established  method  to  build  hollow  metallosupramolecular  (MSM)  structures.                However, utilization of natural-molecule-based  ligands (L) to construct MSM systems was                greatly unexplored until recently, when first bile acid (BA)-based MSM macrocycles Pd3L6                                                             1,2                were  introduced  and  studied  in  our  group.   Additionally,  utilization  of  BAs  provides                possibility for potential biomedical application of the MSM systems because of their inherent                                                                 3                transportation ability and enterohepatic selectivity.  Therefore, our latest study explores novel                                                                                    2+                BA-based tris-pyridyl ligand and its adaptive self-assembly with Pd  resulting in selective                formation of octahedral Pd6L8 (3.6 nm diameter, 6.3 kDa) or first-ever cuboctahedral Pd12L16                (5 nm diameter, 12.6 kDa) MSM cages by regulating the reaction kinetics. These large, Pd-                containing, highly charged (+12 or +24), and water-soluble cages display anticancer activity                and enhanced absorption when employed in vitro on the hepatospheroids of HepG2 cell line.                 Acknowledgements:  Authors  thank  to  grant  Agency  of  Masaryk  University                (MUNI/A/1575/2023).                References:                1.     Jurček, O.; Bonakdarzadeh, P.; Kalenius, E.; Linnanto, J. M.; Groessl, M.; Knochenmuss, R.; Ihalainen,                J. A.;  Rissanen,  K.  Superchiral  Pd 3L 6  Coordination  Complex  and  Its  Reversible  Structural  Conversion  into                Pd 3L 3Cl 6   Metallocycles.   Angew.   Chem.   Int.   Ed.   2015,   54   (51),   15462–15467.                https://doi.org/10.1002/anie.201506539.                2.     Jurček, O.; Nonappa; Kalenius, E.; Jurček, P.; Linnanto, J. M.; Puttreddy, R.; Valkenier, H.; Houbenov,                N.; Babiak, M.; Peterek, M.; Davis, A. P.; Marek, R.; Rissanen, K. Hexagonal Microparticles from Hierarchical                Self-Organization  of  Chiral  Trigonal  Pd 3L 6  Macrotetracycles.  Cell  Rep.  Phys.  Sci.  2021,  2  (1),  100303.                https://doi.org/10.1016/j.xcrp.2020.100303.                3.     Durník, R.; Šindlerová, L.; Babica, P.; Jurček, O. Bile Acids Transporters of Enterohepatic Circulation                for Targeted Drug Delivery. Molecules 2022, 27 (9), 2961. https://doi.org/10.3390/molecules27092961.                                                                                                              97","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO23                 Satureja montana and S. kitaibelii essential oil variability – chemotaxonomic                                                        approach                                   1                    Tanja Dodoš , Smiljana Janković *, Jelica Novaković , Petar D. Marin  and Nemanja                                                                        1                                                                                         1                                                    1,                                                         Rajčević 1                        1  University of Belgrade, Faculty of Biology, Studentski trg 16, Belgrade, Serbia                                   *Corresponding author: smiljana.jankovic@bio.bg.ac.rs                 Satureja montana L. and S. kitaibelii Wierzb. ex Heuff. are aromatic and medicinal plants used                in traditional medicine for different purposes, mostly for urinary, digestive  and pulmonary                disorders. These uses are due to the plants' proven antimicrobial and antioxidant properties.                However, although both species possess medicinal properties, they have different chemical                profiles of essential oils, resulting in different modes of action. Individual essential oils (EOs)                were isolated by simultaneous hydrodistillation and extraction (SDE) method from 17 natural                populations, 14 belonging to S. montana and 3 to S. kitaibelii. These natural populations inhabit                open rocky arid habitats, though the climate differs from the Mediterranean to more continental.                GC/MS and GC-FID analyses show intra- and interpopulation variability of Satureja EOs. In                most populations from S. montana, phenolic compounds (carvacrol and thymol) dominated the                profile, while in S. kitaibelii, those were non-phenolic compounds (limonene, linalool, geraniol                and cis-sabinene hydrate). Populations of both species that grow deeper in the continent with                more arid climates are dominated by non-phenolic compounds in EO, with no or very little                presence of phenolic compounds (< 1%). While dominant compounds did not differ between                continental S. montana and all of the studied S. kitaibelii populations, CDA analysis showed a                clear separation of these two species based on their entire EO profile. These results suggest that                the  phytochemical  profile  of  essential  oils  could  be  used  as  a  taxonomic  marker  for                differentiation between these two species.                 Acknowledgements: Authors thank to the Ministry of Science, Technological Development and                Innovation  of  Republic  of  Serbia  Agency,  Grant  Nos.  451-03-65/2024-03/200178,  451-03-                66/2024-03/200178.                                                                                                                                98","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO24                  The alkane profile as a significant tool for the chemotaxonomy of the genus                                                     Clinopodium L.                                    1,                                                   1                 Smiljana Janković *, Tanja Dodoš , Jelica Novaković , Petar D. Marin , Nemanja Rajčević                                                                     1                                                                                      1                                                                                                         1                        1  University of Belgrade, Faculty of Biology, Studentski trg 16, Belgrade, Serbia                                   *Corresponding author: smiljana.jankovic@bio.bg.ac.rs                 Alkanes are essential components of the plant cuticle, positioned in the epicuticular layer. Their                importance for chemotaxonomy comes from their wide distribution in plants, chemical stability                and easy isolation. Previous studies have shown that alkanes can be excellent markers at the                species level in some very complex groups but are restricted to higher taxonomic levels in                others. The taxonomic problem of  the genus  Clinopodium L. is that formerly separate  and                morphologically distinct genera have been merged into a single genus. This study aims to                evaluate the variability of  alkane profiles in the genus  Clinopodium and to assess whether                alkanes  can  contribute  to  solving  this  taxonomic  problem.  Alkanes  were  isolated  from  24                populations  of  ten  Clinopodium  taxa  from  the  Balkans.  Most  of  the  tested  populations                comprised three pooled samples to characterise the variability of each population better. The                solvent was n-hexane and the samples were analysed by GC/MS and GC/FID. Along with 15                n-alkanes commonly found in cuticular waxes, a rare series of iso- and anteiso-alkanes were                also detected in a significant percentage.  Tritriacontane (n-C33) had the highest concentration                in 52 of 62 samples. In the remaining samples, hentriacontane (n-C31) was the most abundant                alkane and all of these taxa are from the former genus Acinos Mill. In former Acinos taxa, all                populations showed intrapopulation variability concerning the dominant alkane (n-C33 or n-                C31), separating them from the other studied taxa. Discriminant analysis (DA) with the taxa as                groups  showed  a  clear  separation  into  five  groups.  n-C33,  n-C31  and  iso-C31  showed  the                greatest influence on the separation of the taxa. The DA test also showed that the alkane profile                is an excellent marker for delimiting the former genus Calamintha Mill species, a particularly                morphologically  complex  group.  These  results  suggest  that  alkanes  could  be  used  in  the                taxonomy of the genus Clinopodium.                 Acknowledgements: Authors thank the Ministry of Science, Technological Development and                Innovation of the Republic of Serbia, Grant Nos. 451-03-65/2024-03/200178, 451-03-66/2024-                03/200178.                                                                                                                          99","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO25                     Anticholinesterase activity of methanolic extract of Amorpha fruticosa                         flowers and isolation of putrescine and spermidine derivatives                                                                                           1                                                                                                        1                                                                        1                                                     1                                    1,                  Dagmar Jankovská *, Nikol Jurčová , Renata Kubínová , Emil Švajdlenka , Jiří Václavík ,                                                                   1,                                                     Milan Malaník *                 1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého 1946/1,                                               612 00 Brno, Czech Republic                       *Corresponding authors: jankovskad@pharm.muni.cz; malanikm@pharm.muni.cz                 Amorpha fruticosa L. is a leguminous shrub indigenous to North America and later introduced                to Asia as a windbreak and to Europe as an ornamental shrub. Previous phytochemical studies                revealed that it was rich in rotenoids, stilbenes, flavanones, and isoflavones, some of which                                                                                         1                exhibited  significant  cytotoxic,  antidiabetic,  and  antimicrobial  activities.   The  methanolic                extract of flowers  exhibit interesting inhibition activity of cholinesterases. The isolation of                substances responsible for this activity was the goal of our work.                Ethyl-acetate extract was separated on silica gel by column chromatography, then selected                fractions were purified by means of semi-preparative HPLC with a UV/VIS detector. Five                putrescine and spermidine derivatives were obtained from ethyl-acetate extract of the flowers                of A. fruticosa. Isolated compounds were identified by UV, HRMS, and NMR. After evaluation                of the results of spectroscopic methods and comparing them with the literature, the substances                                                                                                 6                                                                                          1                were  identified  as  two  derivatives  of  putrescine:  mongolicine  A  (or  N -(E)-N -(Z)-di-p-                                              1                coumaroylputrescine)  and  N ,N -(E)-di-p-coumaroylputrescine;  and  three  derivatives  of                                                 6                                                    1                                                                                                  1                                                               10                                                                                                      5                                                            5                spermidine: safflospermidine B (or N -(E)-N ,N -(Z)-tri-p-coumaroylspermidine), N ,N -(Z)-                  10                N -(E)-tri-p-coumaroylspermidine and the last compound was obtained in the largest amount                                                                                                     10                                                                                              1                                                                                                  5                and based on the spectroscopic data, it was determined as „all trans“ isomer - N ,N ,N -(E)-                tri-p-coumaroylspermidine.                                           2                A.  fruticosa  contains  several  promising  substances  and  should  be  further  investigated. The                anticholinesterase activity of isolated compounds was measured. The interaction of the studied                compounds  with  the  target  enzymes  was  studied  using  molecular  docking.  Isolated                phenolamides  have  specific  functions  in  plant  development  and  defence,  as  metabolic                intermediates, and final products.  These compounds were isolated from A. fruticosa, Fabaceae                                                2                for the first time.                 References:                1.     Kozuharova, E. et al. A Noxious Invasive Alien Plant in Europe or a Medicinal Plant against Metabolic                Disease? Front. Pharmacol. 2017, 8, 333, https://doi: 10.3389/fphar.2017.00333                2.     Bassard, J.E. et al. Phenolamides: Bridging polyamines to the phenolic metabolism.  Phytochemistry.                2010, 71,16,1808-1824, https://doi.org/10.1016/j.phytochem.2010.08.003                                                                                                                  100","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO26                     Exploring structure-activity-relationships of insect deterrent saponins                                      Jincheng Shen *, Pablo D. Cardenas , Søren Bak                                                                         1                                                                                     1                                                   1,                  1  University of Copenhagen, Department of Plant and Environmental SciencesFrederiksberg                                                     C 1871, Denmark                                          *Corresponding author: shen@plen.ku.dk                 Brassicaceae plants are widely distributed around the world and have extremely high economic                value. Plutella xylostella is a highly insecticidal resistant pest that only feeds on Brassicaceae                plants, causing $4-5 billion in annual economic losses. Previous research our group identified                                                                                                         1                a triterpenoid saponin called hederagenin cellobioside that prevents feeding by P. xylostella .                The  genus  Barbarea  is  the  only  known  Brassicaceae  plant  that  accumulates  saponins.  B.                vulgaris has been divided into two different ecotypes with different saponin profiles, where the                G-type is resistant to most crucifer pest insect and the P-type susceptible.  Saponins are 2,3-                oxidosqualene  derived  amphipathic  triterpenoid  glycosides  that  can  help  plants  prevent                                                                                                     2                herbivores  consumption  by  affecting  cell  membranes  and  imparting  a  bitter  flavor .  The                oxidosqualene cyclase (OSC) LUP2 mainly produces lupeol, and is high expressed in insect-                susceptible B. vulgaris P-type plants, whereas the OSC LUP5 produces β-amyrin and α-amyrin,                and is high expressed in resistant G-type plants . B. vulgaris can therefore be developed into an                                                             3                ecological model system to metabolic engineer and evaluate saponins potential as biopesticide.                Saponins’  toxicity  is  determined  both  by  the  triterpenoid  backbone  structure  and  the                glycosylation  pattern  and  if  it  is  hydroxylated  at  C23.  To  determine  structure-activity-                relationships for saponins toward  P. xylostella  larvae, LUP5 was overexpressed in  the two                genotypes  and  CYP72A552  (C-23  hydroxylation)  was  knocked  down  in G-type. We  found                LUP5 overexpression in the P-type and silencing of CYP72A552 in the G-type changed the                saponin profiles and the resistance level to P. xylostella.                In conclusion, using metabolic engineering, we showed that the saponin profile in B. vulagrais                can be altered, and that this significantly affects its resistance level to feeding by P. xylostella                larvae.  Knowledge  of  the  structure-activity-relationships  of  saponins  towards  agricultural                economically important insect pest is important for the future development of biopesticides for                the  Green Transition  and  for  providing  biosolutions  for  a  more  sustainable  food  and  feed                production system.                 Acknowledgements: Authors thank the Novo Nordisk Foundation, Distinguished Investigator                grant,  NNF  200C0060298.,  EcoSap:  triterpenoid  saponins  as  green  solutions  for  future                sustainable food production; China Scholarship Council, 202108330041.                 References:                1.     Kuzina, V.; Ekstrøm, C. T.; Andersen, S. B.; Nielsen, J. K.; Olsen, C. E.; Bak, S. Identification of Defense                Compounds  in  Barbarea  vulgaris  against  the  Herbivore  Phyllotreta  nemorum  by  an  Ecometabolomic                Approach  Plant Physiol. 2009, 151 (4), 1977-1990. DOI: https://doi.org/10.1104/pp.109.136952.                2.     Augustin, J. M.; Kuzina, V.; Andersen, S. B.; Bak, S. Molecular activities, biosynthesis and evolution of                triterpenoid   saponins.     Phytochemistry    2011,     72      (6),     435-457.     DOI:                https://doi.org/10.1016/j.phytochem.2011.01.015.                3.     Khakimov,  B.;  Kuzina,  V.;  Erthmann,  P.  Ø.;  Fukushima,  E.  O.;  Augustin,  J.  M.;  Olsen,  C.  E.;                Scholtalbers, J.; Volpin, H.; Andersen, S. B.; Hauser, T. P.; et al. Identification and genome organization of saponin                pathway genes from a wild crucifer, and their use for transient production of saponins in Nicotiana benthamiana.                Plant J. 2015, 84 (3), 478-490. DOI: https://doi.org/10.1111/tpj.13012.                                                                                                             101","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO27                     Steroidal molecules as building blocks in supramolecular and material                                                        chemistry                                Ondřej Jurček 1,2,3, *, Subhasis Chattopadhyay 1,2,3 , Radek Marek                                                                                           2,3                 1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého 1946/1,                                                61200 Brno, Czech Republic                2  Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno,                                                      Czech Republic                   3  CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5,                                                62500 Brno, Czech Republic                                       * Corresponding author: jurceko@pharm.muni.cz                 Bile acids (BA) belong to a group of triterpenoid steroidal molecules. The BAs are the main                component of bile. Once the bile is excreted into the small intestine, the BAs form nanosized                mixed  micelles  with  lipophilic  nutrients  mediating  their  digestion,  transport  through  ileal                                                                                1                membrane,  and  distribution  within  the  enterohepatic  circulation.   Moreover,  the  BAs  have                intriguing physicochemical properties such as molecular rigidity, amphiphilicity, chirality, and                possessing hydroxyls and carboxylic acid groups they provide a wide range of possibilities for                chemical  transformations  aiming  at  various,  mostly  pharmacy-related,  applications.   In  our                                                                                                   2                approach, we also set a goal to understand their behavior from the metallo-supramolecular point                of view. We utilized the BAs as building blocks of coordinating ligands which can form large                supramolecular self-assemblies. We have mastered the preparation of many large coordination                species and further advanced to control the formation of higher self-organized structures, micro-                or nanoparticles (Figure),  which opens an exciting area of intriguing applications.                                         3                               References:                1.  Durník, R.; Šindlerová, L.; Babica, P.; Jurček, O. Bile Acids Transporters of Enterohepatic Circulation for                    Targeted Drug Delivery. Molecules 2022, 27 (9), 2961. https://doi.org/10.3390/molecules27092961.                2.  Li, H.; Valkenier, H.; Judd, L. W.; Brotherhood, P. R.; Hussain, S.; Cooper, J. A.; Jurček, O.; Sparkes, H. A.;                    Sheppard, D. N.; Davis, A. P. Efficient, non-toxic anion transport by synthetic carriers in cells and epithelia.                    Nat. Chem. 2016, 8, 24-32. https://doi.org/10.1038/NCHEM.2384                3.  Jurček, O.; Nonappa; Kalenius, E.; Jurček, P.; Linnanto, J. M.; Puttreddy, R.; Valkenier, H.; Houbenov, N.;                    Babiak, M.; Peterek, M.; Davis, A. P.; Marek, R.; Rissanen, K. Hexagonal microparticles from hierarchical                    self-organization  of  chiral  trigonal  Pd 3L 6  macrotetracycles.  Cell  Rep.  Phys.  Sci.  2021,  2  (1),  100303.                    https://doi.org/10.1016/j.xcrp.2020.100303.                                                                                                              102","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO28                  Assessment of polyphenol variability, flavonoids, phenolic acids, pigments,                       and antioxidant activity across two growth stages of ginkgo leaves                                             Iva Jurčević Šangut*, Dunja Šamec                    University North, Department of Food Technology, Trg dr. Žarka Dolinara 1, Koprivnica,                                                          Croatia                                          *Corresponding author: ijurcevic@unin.hr                 Ginkgo  leaves  are  esteemed  for  their  abundance  of  specialized  metabolites,  including                flavonoids, terpenoids, and trilactones, known for their manifold medicinal properties like anti-                inflammatory,  antiviral,  antifungal,  and  anticancer  effects.  Nonetheless,  the  composition  of                these  metabolites  is  subject  to  various  factors  such  as  plant  variety,  growth  stage,  and                                                            1                environmental  conditions  during  cultivation .  This  study  aimed  to  compare  the  levels  of                phenols,  flavonoids,  phenolic  acids,  pigments,  and  antioxidant  activity  in  Ginkgo  leaves                harvested in May and October. The analysis revealed significant differences in all phenolic                components, with higher concentrations of polyphenols and flavonoids found in October leaves.                Conversely,  May  leaves  exhibited  higher  levels  of  phenolic  acids  and  antioxidant  activity.                While these findings suggest the promising potential of autumn leaves as a source of bioactive                compounds, further research is warranted to thoroughly explore the phytochemical composition                of Ginkgo leaves during the autumn season.                 Acknowledgements: This  work  was  funded  by  the  Croatian  Science  Foundation  under  the                project: “Biflavonoids role  in plants:  Ginkgo biloba  L. as  a model system” (UIP-2019-04-                1018).                 References:                1.     Šamec, D.; Karalija, E.; Dahija, S.; Hassan, S.T.S. Biflavonoids: Important Contributions to the Health                Benefits of Ginkgo (Ginkgo biloba L.). Plants 2022, 11 (10), 1381. 10.3390/plants11101381                                                                                                                                    103","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO29                  Assessment of cytotoxicity and antioxidant capacity of Ranunculus arvensis                                            L. and isolation of its flavonoids                                                                                    4                                                                    3                                                     2                                                                                                       1                    Çiğdem Kahraman *, Kübra Şener , Michal Šoral , Golshan Zare , Didem Şöhretoğlu                                      1,                 1  Hacettepe University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Türkiye                        2  Gazi University, Faculty of Science, Department of Biology, Ankara, Türkiye                   3  Slovak Academy of Sciences, Institute of Chemistry, Analytical Department, Bratislava,                                                      Slovak Republic                 4  Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara,                                                          Türkiye                                     *Corresponding author: cigdemm@hacettepe.edu.tr                 The genus Ranunculus L. (Ranunculaceae) is represented by 84 species in Flora of Turkey and                is recorded to be used for the treatment of muscle and joint pain, rheumatism, psoriasis, wounds,                and edema. It is also used worldwide for asthma, fever, and gout, as well as skin disorders in                homeopathic preparations. There are species recorded to be used against various types of cancer                in Traditional Chinese Medicine.                This study aimed to evaluate the cytotoxic and antioxidant capacity of Ranunculus arvensis L.                and  isolate  its  secondary  metabolites.  The  methanolic  extract  of  the  whole  plant  was                successively  partitioned  with  n-hexane  and  n-butanol.  The  n-butanol  sub-extract  was                fractionated by polyamide column chromatography which yielded 8 fractions. The methanol                and n-butanol extracts along with mentioned fractions were tested for their cytotoxicity by MTT                assay on the A549 cell line and for antioxidant capacity by the ferric reducing antioxidant power                (FRAP) and the cupric reducing antioxidant capacity (CUPRAC) assays. Only one fraction,                which was not sufficient for the isolation studies, displayed moderate activity with 49,21 ±                2,37% inhibition at a dose of 200 µg/ml. Two of the fractions exhibiting antioxidant capacity                were  used  for  further  chromatographic  separation,  allowing  the  isolation  of  isovitexin,                hyperoside, isoorientin, and gossypitrin as well as a mixture of isoorientin-2''-O-[6'''-O-E-p-                coumaroyl-beta-D-glucopyranoside]       and     isoorientin-2''-O-[6'''-O-E-p-feruloyl-beta-D-                glucopyranoside]. These metabolites were isolated from R. arvensis for the first time.                 Acknowledgements:  Authors  thank  to  Hacettepe  University  Scientific  Research  Projects                Coordination Unit, Project Number: TYL-2021-19188, Phytochemical and Biological Activity                Studies on Ranunculus arvensis L.; and to the Grant Agency of the Ministry of Education,                Research, Development and Youth of the Slovak Republic VEGA (project no. 2/0118/23).                                                                                                                     104","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO30                    LC-(HR)MS/MS method to determine cucurbitacins in Cucurbitaceae –                                            Survey in Dutch retail market                                                                           2                                                                                          1                                               1                             1,                 Sylvia Kalli *, Patrick Mulder , Ilaria di Marco Pisciottano , Elena de Vries , Josipa Grzetic                                                         Martens 1                   1  Wageningen Food Safety Research, Part of Wageningen University \& Research, European                      Union Reference Laboratory plant toxins and mycotoxins, P.O. Box 230, 6700 AE,                                               Wageningen, the Netherlands                  2  Department of Chemistry, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici                                                       (Naples), Italy                                         *Corresponding author: sylvia.kalli@wur.nl                 Cucurbitacins (CBCs) constitute a group of oxygenated  triterpenoid substances, commonly                found in the family of Cucurbitaceae. They are predominantly known for their bitterness and                toxicity, yet some exhibit positive bioactivities. Several human-poisoning incidents from CBCs                have  been  reported  over  the  last  decades.  In  2018,  two  women  developed  acute  toxicity                symptoms upon consumption of a pumpkin soup made of bitter pumpkin and had hair loss                                                                             3                weeks later   1, 2 . Furthermore, fatal cases in Germany (2015)   and Twente, the Netherlands                (2021) potentially linked to CBCs ingestion were reported. Breeding has minimized the CBCs                content  in  edible  species  but  crossbreeding  with  bitter/wild  types  (for  example  ornamental                pumpkins) or uncontrolled growth can strongly elevate concentrations. The aim of the study                presented herein was to develop  a method  to detect CBCs in the fruits of three species of                Cucurbitaceae (i.e. zucchini, pumpkin and cucumber). Additionally, the study aimed to perform                a market survey analysing the presence of CBCs in products obtained from local (super)markets                and homegrown products. A straightforward method for the determination of nine CBCs was                developed. A simple extraction of CBCs from the food matrices followed by detection and                quantification using LC-MS/MS was employed. The method was intra-day validated, showing                acceptable precision and recoveries. The obtained LOQs in the two matrices were in the range                of 10-25 μg/kg. Fifty samples were analysed, from retail, local market and homegrown origin,                including five ornamental pumpkin samples and four samples of bitter zucchini, three of which                were  from  an  intoxication  case.  The  majority  of  samples  were  from  sweet  varieties  and                contained negligible CBC levels. In contrast, ornamental pumpkin and bitter zucchini samples                exhibited  significant  CBC  concentrations,  reaching  up  to  1850  mg/kg  fresh  weight.  High-                resolution mass spectrometry identified two new CBCs in these samples. Because ornamental                pumpkins  and  bitter  zucchini  cannot  always  be  easily  visually  discriminated  from  sweet                varieties, accidental consumption cannot be excluded.                References:                1.     Assouly, P. Hair loss associated with cucurbit poisoning. JAMA dermatology 2018, 154 (5), 617-618.                https://doi.org/10.1001/jamadermatol.2017.6128.                2.     Le Roux, G.; Leborgne, I.; Labadie, M.; Garnier, R.; Sinno-Tellier, S.; Bloch, J.; Deguigne, M.; Boels,                D. Poisoning by non-edible squash: retrospective series of 353 patients from French Poison Control Centers. Clin                Toxicol (Phila) 2018, 56 (8), 790-794. https://doi.org/10.1080/15563650.2018.1424891.                3.     on-line,  S.  Duitser  dood  na  eten  courgette  (German  man  dies  after  eating  zuccini).  Telegraaf  2015,                https://www.telegraaf.nl/nieuws/544262/duitser-dood-na-eten-courgette.                                                                                                              105","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO31                     Chasing molecules - interdisciplinary approaches in modern structure                                                        elucidation                                                 1                                 1                                                                                                      1                                                                                       1                   Jonas Kappen , Tristan Fuchs , S. Phani B. Vemulapalli , Lea Schmitz , Andrej Frolov ,                                                                         2                                          Jeprianto Manurung 1,3,4 , Andria Agusta ,                                                                                5                                                 3,4                                                                        2                     Alexandra N. Muellner-Riehl , Christian Griesinger , Katrin Franke 1,4,6, *, Ludger A.                                                      Wessjohann 1,4, *                   1  Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry (IPB), Halle                                                     (Saale), Germany                    2  Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical                                              Chemistry, Göttingen, Germany                  3  Department of Molecular Evolution and Plant Systematics \& Herbarium (LZ), Institute of                                           Biology, Leipzig University, Germany                  4  German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Germany                    5  Research Center for Pharmaceutical Ingredients and Traditional Medicine, National                                Research and Innovation Agency (BRIN), Jakarta, Indonesia                   6  Institute of Biology/Geobotany and Botanical Garden, Martin Luther University Halle-                                                Wittenberg, Halle, Germany                           *Corresponding author: kfranke@ipb-halle.de; wessjohann@ipb-halle.de                 The  newly  discovered  natural  compound  lumnitzeralactone,  identified  as  an  ellagic  acid                derivative,  was  isolated  from  the  antibacterial  extract  of  the  Indonesian  mangrove  species                Lumnitzera racemosa Willd. The structure of lumnitzeralactone, characterized by a deficit in                protons and a complex  condensed aromatic ring system, was unequivocally determined by                comprehensive spectroscopic analyses, including high-resolution mass spectrometry (HRMS),                    1                1D  H and  C nuclear magnetic resonance spectroscopy (NMR), and 2D NMR techniques,                            13                including the more unusual experiments 1,1-ADEQUATE and 1,n-ADEQUATE. The structural                elucidation was further supported by computer-assisted structure elucidation (CASE system                utilizing ACD-SE), density functional theory (DFT) calculations, and finally by  a two-step                chemical synthesis.                 Acknowledgements:  Financial  support  was  provided  by  the  project  “Indonesian  Plant                Biodiversity  and  Human  Health—BIOHEALTH”  (BMBF  grants  no.  16GW0120K  and                16GW0123),  the  project  ProCognito  by  EFRE  and  the  state  Saxony-Anhalt                (ZS/2018/11/95581)  and  by  the  German Academic  Exchange  Service  (DAAD  project  no.                91653688)                 References:                1.     Kappen et al. Challenging Structure Elucidation of Lumnitzeralactone, an Ellagic Acid Derivative from                the Mangrove Lumnitzera racemosa. Mar. Drugs 2023, 21, 242. https://doi.org/10.3390/md21040242                                                                                                              106","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO32                   The study of the antibacterial activity of agrimony against gram-positive                                                         bacteria                                                                                                      1                                                                1                                          1                                                                              1                        Ema Kostovčíková *, Kamila Dokupilová , Elena Kurin , Silivia Bittner Fialová , Eva                                                             2                                                      Drobná , Pavel Mučaji 1                         1 Comenius University Bratislava, Department of Pharmacognosy and Botany, Faculty                                         of Pharmacy, Odbojárov 10, SK- 832 32 Bratislava                           2 Comenius University Bratislava, Department of  Cell and Molecular Biology of                                 Drugs, Faculty of Pharmacy, Odbojárov 10, SK- 832 32 Bratislava                                          *Corresponding author: kostovcikova2@uniba.sk                  Agrimonia eupatoria (Rosaceae) is a well-known perennial herb widely spread in Europe. Its                secondary  metabolites  comprise  tannins,  flavonoids,  phenolic  acids,  and  triterpenoids.                Agrimony is traditionally used to treat minor skin inflammations and its secondary metabolites                are known for their antimicrobial effects. Still, only a limited number of studies have been                conducted in this field. This study aims to explore the antibacterial properties of agrimony                methanol extract against gram-positive bacteria using isothermal microcalorimetry (IMC). All                biological processes generate or absorb energy in the form of heat. Metabolically active bacteria                produce  heat  that  corresponds  to  their  growth  rate.  IMC  measures  the  heat  of  biological                processes  that  are  directly  related  to  the  speed  at  which  the  given  process  progresses. A                phytochemical analysis of the extract was also performed. Except for IMC, the conventional                broth microdilution method (BMC) to determine the minimal inhibitory concentration (MIC)                and the minimal bactericidal concentration (MBC) has been used. As shown in Fig. 1, the                metabolic activity of MSSA decreases alongside the increasing  agrimony concentration. All                observed parameters (heat flow, total heat, and time to peak) in bacteria exposed to extract,                were  reduced.  The  results  of  both  methods  (IMC  and  BMC)  confirm  the  antimicrobial                properties of agrimony.                                       Acknowledgments: Authors thank Grants VEGA 1/0226/22, VEGA 1/0170/24 and                FAF/19/2024.                                                                                                              107","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO33                       Isoquinoline alkaloids in Corydalis cheilanthifolia cultured in vitro                    Weronika Kozłowska , Monika Bielecka , Marta Stafiniak , Bartosz Pencakowski , Adam                                                         2                                                                                                 2                                       1                                                                          2                                                         2                                               Matkowski , Sylwia Zielińska 1                               1 Department of Pharmaceutical Biology and Biotechnology, Division of                         Pharmaceutical Biotechnology,* Student Scientific Club no K76, Wroclaw Medical                                                University, 50-556 Wroclaw, Poland                              2  Department of Pharmaceutical Biology and Biotechnology, Division of                         Pharmaceutical Biology and Botany, Wroclaw Medical University, 50-556 Wroclaw,                                                              Poland                                     *Corresponding author: weronika.kozlowska@umw.edu.pl                  Isoquinoline alkaloids are specialised metabolites produced by poppy plants (Papaveraceae).                Chelidonium majus is a well-known medicinal plant, widely used in folk medicine and known                for its high content.  A less well known but no less rich source of isoquinoline alkaloids is the                fern leaf corydalis - Corydalis cheilanthifolia. Techniques are being developed to efficiently                extract bioactive substances such as isoquinoline alkaloids under laboratory conditions, using                the possibilities of growing medicinal plants in glass and applying classical biotechnological                procedures. Isoquinoline alkaloids have the potential to be used as effective drugs without side-                effects, with major implications for the prevention and treatment of many inflammatory and                cancerous  diseases.  The  structure  of  natural  compounds  can  be  difficult  to  reproduce  by                chemical  synthesis,  and  only  in  their  original  form  can  they  exhibit  valuable  biological                properties.                  The rationale for using a biotechnological approach lies in the greater control and chance of                isolating and pinpointing the factors at work that are crucial in regulating the biosynthesis and                accumulation of plant metabolites such as isoquinoline alkaloids.                  The  aim  of  this  research  project  is  to  evaluate  the  biosynthetic  pathways  for  isoquinoline                alkaloids  in  plants  and  to  increase  the  efficiency  of  their  extraction.  The  identification  of                potential enzyme candidates involved in the biosynthesis of isoquinoline alkaloids in the plant                C. cheilanthifolia and the in vitro cultivation of selected clones is the first step of the project.                  Acknowledgements: This research is funded by National Science Center of Poland (NCN) #                2023/07/X/NZ7/01628.                                                                                                                              108","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO34                    In vitro culture and phytochemical profiling of Agrostemma githago and                                                   Gypsophila elegans                                                                   2                                                                                                        1                                                                                     1                                       1,                  Weronika Kozłowska *, Weronika Korzeniowska , Karolina Sołowiej , Sylwia Zielińska ,                       Monika Bielecka , Marta Stafiniak , Izabela Nawrot-Hadzik , Adam Matkowski   2                                                        2                                                                                 2                                       2                    1  Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical                Biotechnology, Student Scientific Club no K76, Wroclaw Medical University, 50-556 Wroclaw,                                                          Poland                   2  Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical                         Biology and Botany, Wroclaw Medical University, 50-556 Wroclaw, Poland                                  *Corresponding author: weronika.kozlowska@umw.edu.pl                 Plants from the Caryophyaceae family are a rich source of valuable phytochemicals known for                their  medicinal  but  also  preventive  properties  against  f.  ex.  cardiological  or  civilization                diseases. The  main  groups  of  compounds  found  in  Caryophyllaceae  species  are  saponins,                phytoecdysteroids,  polyphenolic  compounds  including  flavonoids,  and  N-containing                compounds. Agrostemma githago was once a noxious grainfield weed causing mass poisoning                due to its toxic metabolites while Gypsophila elegans is an ornamental plant native to Asia and                Europe. Despite the specialized compounds both species contain ribosome inactivating proteins                type 1 (RIP1) agrostin and gypsophilin, respectively, a specific class of plant protein toxins.                The aim of the study was to 1) verify if the compounds of interest can be produced under the in                vitro conditions of  A. githago and G. elegans in organ and/or  tissue culture,  as well  as 2)                determination  of  conditions  allowing  stable  growth  and  efficient  production  of  specialized                compounds.                The  explants  obtained  from  aseptic  two-week-old  seedlings  were  cultured  on  MS  media                supplemented with plant growth regulators (PGR): kinetin (KIN), benzylaminopurine (BAP),                2-isopentenyladenine (2-iP) and indole-3-acetic acid (IAA) in different concentration.                The effect of PGR on the secondary metabolite profile of both species was monitored using                chromatographic techniques (HPLC-DAD, UHPLC-MS). Flavone C-glycosides orientin and                vitexin  were  identified  in  in  vitro  grown  shoots  as  well  as  saponin  derivatives  including                gypsophilin ones. The influence of PGR on the growth and morphology of both species was                also  recorded  on  the  number  and  length  of  main  and  axillary  shoots  per  explant,  biomass                production, and the spontaneous vitrification occurrence.                In conclusion, A. githago and G. elegans in vitro culture are a feasible and responsive model                for induction of growth and morphogenesis as well as for specialized compound biosynthesis.                 Acknowledgements: This research is funded by National Science Center of Poland (NCN) #                2020/39/I/NZ7/01515.                                                                                                               109","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO35                   Oregano and red wine: an antioxidant combination that leads to synergy                                                        1                                                                                        2                                                                          1                    Elena Kurin *, Kamila Dokupilová , Ema Kostovčíková , Iveta Čičová , Silvia Bittner                               1,                                                   1                                                                1                                                                               1                                           Fialová , Milan Nagy , Pavel Mučaji                    1  Comenius University Bratislava, Department of Pharmacognosy and Botany, Faculty of                                    Pharmacy, Odbojárov 10, 832 32 Bratislava, Slovakia                   2  National Agricultural and Food Centre, Research Institute of Plant Production, 921 01                                                     Piešťany, Slovakia                                        *Corresponding author: elena.kurin@uniba.sk                 Origanum vulgare L., known as oregano, is a popular culinary herb. It is a frequent ingredient                for flavoring in Mediterranean cuisine. However, in addition to its taste and smell, which are                given by the content of essential oil, it is a plant with a wide potential of biological activities.                Red wine is known for many as an integral part of a South Europe dinner and a rich source of                polyphenols. As the oregano and red wine combination in meals is quite common, we decided                to study mutual interaction in five antioxidant  assays with different  mechanisms of action.                Lyophilizates  of  red  wine  (cuvée  Blaufränkisch  and  Dunaj,  Matula  2021,  BFD)  and  water                infusion from oregano leaves (OV) were mixed in three ratios: 1:1, 1:10, and 10:1. Rosmarinic                acid was used as a positive control. Combination index (CI) was used for quantification of                interaction, where CI =, ˂ or ˃ 1, which means additive, synergic or antagonistic reaction. In                ABTS and DPPH assays, where hydrogen atom transfer is the  main mechanism of  action,                synergy was observed in BFD:OV 1:1 (ABTS: CI = 0.68; DPPH: CI = 0.76) and 10:1 (ABTS:                CI =0.72; DPPH CI = 0.52) combination, while 1:10 combination was slightly antagonistic                (ABTS: CI = 1.33) or nearly additive (DPPH: CI = 1.06). In the FRAP assay, where the main                mechanism of action is based on single electron transfer, the mixture exhibited slight synergy                in all combination ratios (BFD:OV 1:1 CI = 0.91; 10:1 CI = 0.90; 1:10 CI = 0.86). Synergy was                observed also in Fenton reaction based assays with H2O2 with 1,10-phenantroline (BDF:OV 1:1                CI = 0.75; 10:1 CI = 0.38; 1:10 CI = 0.67) an salicylic acid (BDF:OV 1:1 CI = 0.85; 10:1 CI =                0.66; 1:10 CI = 0.46). Phytochemical analysis of total polyphenols in both lyophilizates was                performed spectrophotometrically, where BFD has 7.7% and OV 18.4% of total polyphenols.                Even though the research brought several questions to the interpretation of the interaction, the                presence  of  different  synergistic  combination  of  BFD  and  OV  confirms  that  mixtures  of                antioxidants are more active, as if they are alone.                 Acknowledgments: This work was supported by grants APVV-19-0056 and VEGA 1/0170/24.                                                                                                                     110","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO36                    Evaluation of Antidiabetic Properties of Roselle plant extracts (Hibiscus                                                       sabdarifa L.)                                                              1,2                                                                               1                                           Slavomír Kurhajec *, Karel Šmejkal                   1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk university, Palackého třída                                               1946/1, 612 00 Brno, Czechia                   2  Department of Paharmaceutical Technology, Pharmacognosy and Botany, University of                        Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia                                      *Corresponding author: slavomir.kurhajec@uvlf.sk                    The Roselle plant (Hibiscus sabdariffa L.) produces various substances in its metabolism that                have  potential  antidiabetic  properties. As  these  metabolites  are  characterized  by  different                polarity and extractability into different solvents the experiment was designed for three types                of solvent (water, methanol, chloroform). In the prepared extracts, selected substances (total                polyphenols  and  total  anthocyanins)  which  are  assumed  to  be  co-carriers  of  the  observed                antidiabetic  activity  were  quantitatively  determined. The  highest  level  of  total  polyphenols                expressed  as  gallic  acid  equivalent  (10.96  ±  0.13  mg/g)  and  anthocyanins  expressed  as                cyanidin-3-glucoside (0.28 ± 0.01 mg/g) were detected in the aqueous extract. Antioxidants                contribute to the protection of the pancreatic β cells from oxidative damage and at the same                time reduce the prevalence of diabetes complications. That is why the antioxidant activity of                the extracts was evaluated using two methods. In the DPPH assay the aqueous extract showed                the  highest  antioxidant  activity  (0.40  ±  0.03  μmol Trolox /μl)  and  in  the  FRAP  assay  the                                                                          ®                methanolic extract (58.67 ± 0.92 FRAP/ml). By inhibiting α-amylase, the amount of resorbable                glucose  from  the  intestine  is  significantly  reduced,  which  limits  the  development  of                postprandial hyperglycemia in diabetics. The water extract of roselle plant was in vitro able to                inhibit α-amylase (70.63 ± 1.59%). The prepared aqueous extract thus provides great potential                in the complementary treatment of patients suffering from diabetes.                   References:                 1.     Bule, M. Albelbeisi, A.H. Nikfar, S. Amini, M. Abdollahi, M. The antidiabetic and antilipidemic effects                of Hibiscus sabdariffa: A systematic review and meta-analysis of randomized clinical trials. Food Res Int. 2024,                130, 1 - 11. https://doi:10.1016/j.foodres.2020.108980                                                                                                                      111","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO37                   Evaluating the anti-inflammatory potential of prenylated (iso)flavonoids                     from Ficus cyathistipula in an in vitro model of inflammatory response                                                               1                                                                               2                                                                                           2,3                                            1,                              Jovana Lisičić *, Milan Malaník , Renata Hežová , Jan Hošek                   1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého třída                                                    1946/1, 612 00 Brno                    2  Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova                                                    296/70, 621 00 Brno                 3  Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Palackého                                                 třída 1946/1, 612 00 Brno                                          *Corresponding author: 507401@muni.cz                 In recent years, there has been renewed interest in natural treatments and plants as potential                sources  of  new  drugs  for  the  treatment  of  inflammatory  diseases.  Prenylated  phenolics,                a diverse group of natural compounds found in various plants, are known for their significant                anti-inflammatory potential. Therefore, roots of Ficus cyathistipula  Warb. (Moraceae) were                subjected to extensive chromatographic separation leading to the isolation of twelve prenylated                (iso)flavonoids of which five compounds were isolated from plant material for the first time.                Their potential anti-inflammatory effect was investigated in an in vitro study. The first screening                was conducted on the human leukemia monocytic cell line THP1-Blue™ NF-κB stimulated                with lipopolysaccharide. The anti-inflammatory potential was evaluated as the inhibition of                NF-κB, one of the key pro-inflammatory transcriptional factors, using the QUANTI-Blue™                colorimetric enzyme assay.                Results demonstrated that seven prenylated (iso)flavonoids showed the same or better effect on                the inhibition of the NF-κB signaling pathway compared to the standard drug prednisolone. The                inhibition of nuclear translocation of NF-kB, as one of the potential mechanisms of action, was                investigated in the two most active compounds. The obtained results opened the door for further                study of the anti-inflammatory potential of these compounds.                                                                                                                                  112","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO38                       Antimicrobial activity of two orange varieties essential oils for the                                               valorisation of peel wastes                                                                   4                Marta Lo Vetere *, Valeria Iobbi , Christina Sykora , Anna Paola Lanteri , Giovanni Minuto ,                                                                                                         2                                1,                                                1                                                                                       2                                                                                 1                                                                   3                                         2                           Andrea Minuto , Nunziatina De Tommasi , Angela Bisio , Sabine Glasl 4                              1 Deparment of Pharmacy, University of Genova, Viale Cembrano 4                   2  Centro di Sperimentazione e Assistenza Agricola (CeRSAA), Regione Rollo 98, Albenga                3  Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano,                                                           Italy                   4  Department of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2                                    *Corresponding author: marta.lo.vetere@edu.unige.it                 Citrus fruits are one of the most important commercial crops cultivated all over the world. These                fruits have attracted attention for their medicinal value. Citrus fruits contain a wide range of                bioactive  components,  and  by-products  obtained  from  Citrus  waste  are  recognized  as  a                renewable source of valuable substances for application in the pharmaceutical industry [1].                Essential  oils  are  natural  plant  constituents  known  for  their  antimicrobial,  antifungal  and                antioxidant properties [2]. In this study, essential oils from two citrus species (Citrus sinensis                “Navel orange” and Citrus sinensis “Tarocco”) were investigated for their antimicrobial activity                against plant infecting agents. Fruits were collected at the ripening stage. After squeezing the                juice, the orange peels were removed and dried in the sun. The peels were cut into small pieces                and subjected to hydro-distillation using a Clevenger apparatus. The essential oils were dried                over anhydrous magnesium sulphate and stored in sealed vials until the characterization by                 GC/EI-MS analysis. The identification of components was based on the comparison of their                mass spectral fragmentation pattern with databases. Subsequently, a screening of the biological                activity of the essential oils was conducted on various bacterial and fungal strains, including                those known to infest numerous varieties of fruits, vegetables, and ornamentals. This analysis                aimed to study and compare the efficacy and potential impact of the two essential oils on the                development and growth of the tested pathogens. The results of this screening provide valuable                insights into the antibacterial and antifungal activity of the two Citrus essential oils and their                potential utility as agrochemicals.                 References:                2.     Sharma, K.; Mahato, N.; Cho, M. H.; Lee, Y. R. Converting Citrus Wastes into Value-Added Products:                Economic    and    Environmently   Friendly   Approaches.   Nutrition   2017,   34,   29–46.                https://doi.org/10.1016/j.nut.2016.09.006.                3.     Brah,  A.  S.;  Armah,  F.  A.;  Obuah,  C.;  Akwetey,  S.  A.;  Adokoh,  C.  K.  Toxicity  and  Therapeutic                Applications of Citrus Essential Oils (CEOs): A Review. International Journal of Food Properties 2023, 26 (1),                301–326. https://doi.org/10.1080/10942912.2022.2158864.                                                                                                                 113","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO39                     Constituents of hawthorn (Crataegus spp.) and their ability to inhibit                                                intestinal α-glucosidases                                  Zuzana Lomozová*, Patrícia Harčárová, Přemysl Mladěnka                     Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského                                      1203/8, Hradec Králové 500 03, Czech Republic                                        *Corresponding author: lomozovz@faf.cuni.cz                 Hawthorn (Crataegus spp., Rosaceae) is an old traditional and popular herbal medicine that                contains a broad spectrum of substances (e.g., flavonoids, triterpenic acids, amines or phenolic                                                                                            1                acids) and has been used for the treatment of different cardiovascular diseases . In traditional                Chinese medicine, it has been administered for the treatment of hyperglycaemia for a long time.                Even though several studies have confirmed the antidiabetic effects of Crataegus extract, the                mechanism of action is not known. A possible clue can be α-glucosidase, the main enzyme                catalysing the final step of carbohydrate hydrolysis to glucose in the digestive system. Inhibition                of this enzyme is associated reduction in glucose absorption and glycaemia 2, 3 .                For this reason, several major constituents of hawthorn were tested for their ability to block α-                glucosidases. Both yeast and mammalian (rat) glucosidases were used. While triterpenic and                phenolic acids showed a rather mild effect, flavonoids and especially flavonoid glycosides were                potent inhibitors. The most effective compound was spireoside, which inhibited α-glucosidase                more effectively than the registered drug acarbose, which was chosen as the standard agent.                There  were  significant  discrepancies  between  yeast  and  mammalian  (rat)  intestinal  α-                glucosidase inhibition. Yeast α-glucosidase does not appear to be a suitable model to predict                effect in humans.                 Acknowledgements: Authors thank to the Charles University project Cooperatio.                 References:                 1.  Cloud, A.; Vilcins, D.; McEwen, B. The effect of hawthorn (Crataegus spp.) on blood pressure: A systematic                    review. Adv. Integr. Med. 2020, 7 (3), 167-175. https://doi.org/10.1016/j.aimed.2019.09.002.                 2.   Li, T.; Fu, S.; Huang, X.; Zhang, X.; Cui, Y.; Zhang, Z.; Ma, Y.; Zhang, X.; Yu, Q.; Yang, S.; Li, S.                    Biological properties and potential application of hawthorn and its major functional components: A review.                    J. Funct. Foods 2022, 90, 104988. https://doi.org/10.1016/j.jff.2022.104988.                 3.  Aierken, A.; Buchholz, T.; Chen, C.; Zhang, X.; Melzig, M. F. Hypoglycemic effect of hawthorn in type II                    diabetes mellitus rat model. J. Sci. Food Agric. 2017, 97 (13), 4557-4561. https://doi.org/10.1002/jsfa.8323.                                                                                                                         114","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO40                    Anti-inflammatory activity and phytochemical profile of different plant                      material from Rhododendron luteum Sweet – comparative analysis                            Lena Łyko, Agnieszka Łubek-Nguyen*, Marta Olech and Renata Nowak                     Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street,                                                   20-093 Lublin, Poland                                  *Corresponding author: agnieszka.lubek-nguyen@umlub.pl                 Terpenes  are  plant  secondary  metabolites  known  for  their  anti-inflammatory  potential.                Rhododendron  luteum  Sweet  (RL)  was  previously  reported  to  present  anti-inflammatory                        1                activity.  This species is a deciduous shrub that grows to a height of 4 m with oblanceolate                leaves and yellow, exquisitely scented flowers.                                                             2                 The present research investigates the terpene profile and anti-inflammatory activity of ASE                (accelerated solvent extraction) extracts obtained from RL leaves, flowers, fruits, and woody                stems. Samples were purified using SPE (solid phase extraction) before the chromatographic                analysis. LC-ESI-MS/MS (liquid chromatography with electrospray ionization coupled with                triple quadrupole mass spectrometry) analysis revealed the presence of pentacyclic triterpenes                and phytosterols. Samples were also subjected to in vitro anti-inflammatory activity assays                using  spectrophotometric  methods.  IC50  (half  maximal  inhibitory  concentration)  values  for                investigated extracts, as a measure of their potency in inhibiting the specific activity of enzymes                lipoxygenase, hyaluronidase, and xanthine oxidase, were determined. Additionally, the mode                of lipoxygenase and xanthine oxidase inhibitory was determined. Significant differences in                enzymes inhibition were observed depending on the tested sample.                The rich abundance of compounds known for their pharmacological potential and the observed                high anti-inflammatory activity of the extracts obtained from various RL organs encourage                further studies of this species. Based on the obtained results, it can be concluded that R. luteum                is a valuable source of biologically active pentacyclic terpenes and phytosterols.                 References                1.     Łyko, L.; Olech, M.; Nowak, R. LC-ESI-MS/MS Characterization of Concentrated Polyphenolic                Fractions from Rhododendron Luteum and Their Anti-Inflammatory and Antioxidant Activities. Molecules 2022,                27 (3). https://doi.org/10.3390/molecules27030827.                2.     Sawidis, T.; Theodoridou, T.; Weryszko-Chmielewska, E.; Bosabalidis, A. M. Structural Features of                Rhododendron Luteum Flower. Biologia (Bratisl). 2011, 66 (4), 610–617. https://doi.org/10.2478/s11756-011-                0059-5.                                                                                                                            115","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO41                          Optimization of extraction of bioactive pentacyclic triterpenes                                          and polyphenols from rose roots                     Agnieszka Łubek-Nguyen*, Marta Olech, Natalia Nowacka-Jechalke, Justyna Wójcik                     Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street,                                                   20-093 Lublin, Poland                                  *Corresponding author: agnieszka.lubek-nguyen@umlub.pl                 Rose roots contain several groups of bioactive metabolites e.g. triterpenes, flavonoids, and                tannins and constitute a source of herb medicines described in Chinese Pharmacopoeia. In Asian                medicine, they have been used e.g. to treat diabetes, diarrhoea, pain and chronic inflammatory                diseases. However, in Europe, rose roots are almost unused and treated as crop waste. Few                experimental studies indicated the high pharmacological potential of extracts from this plant                material. They were found to demonstrate antioxidant, antiproliferative, anti-inflammatory and                antihyperglycemic properties (Giménez et al., 2015). Therefore, this study aimed to determine                the optimal conditions for obtaining pentacyclic triterpenes and polyphenols from Rosa rugosa                root using relatively simple, cheap and eco-friendly methods.                Ultrasound-assisted  extraction  (UAE;  30  min,  60°C)  and  16  extractants  (different                water/methanol/acetone  ratios)  were  tested. The  content  of  metabolites  in  the  samples  was                determined using LC-ESI-MS/MS and spectrophotometric methods. Moreover, antioxidant and                enzyme inhibitory activity was assayed. It was revealed that the acetone, acetone-methanolic                and acetone-water or methanol-water extract had the highest triterpene and polyphenol content,                respectively  (130.24  mg/g  DW  and  63.76  mg/g  DW,  respectively).  However,  the  highest                extraction  yield  was  obtained  using  a  mixture  of  solvents:  water,  acetone,  methanol.  The                strongest radical scavenging activity was presented by extracts where the eluent contained water                and  more  than  60%  of  organic  solvent  (134.29-152.35  µg  of  Trolox/mg).  The  highest                glucosidase  inhibitory  ability  was  observed  for  sample  obtained  with  50%  methanol.  LC-                MS/MS  analysis  revealed  large  amount  of  catechin  (17.14  mg/g  DW)  and  other  bioactive                polyphenols e.g. quercetin, hyperoside, isoquercetin, quercitrin, as well as, gallic, chlorogenic,                protocatechuic, p-coumaric and salicylic acid.                 References                1.     Giménez,  E.;  Juan,  M.  E.;  Calvo-Melià,  S.;  Barbosa,  J.;  Sanz-Nebot,  V.;  Planas,  J.  M.  Pentacyclic                triterpene in Olea europaea L: A simultaneous determination by high-performance liquid chromatography coupled                to mass spectrometry. J. Chromatogr. A, 2015, 1410, 68-75. https://doi.org/10.1016/j.chroma.2015.07.064.                                                                                                                           116","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO42                 α-Glucosidase inhibitory activity and phytochemical profile of fruit extracts                                               of Czech Sorbus endemics                                              Milan Malaník*, Renata Kubínová                     Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého tř.                                            1946/1, 612 00 Brno, Czech Republic                                      *Corresponding author: malanikm@pharm.muni.cz                 Sorbus  L.  sensu  lato  (Rosaceae)  comprises  over  250  species  widespread  throughout  the                                      1                Northern Hemisphere.  However, taxonomy is very complicated due to the ability of apomixis                and hybridization of Sorbus species. The landscape of the Czech Republic provides a unique                environment that enabled the creation of several endemic microspecies  that are sometimes                referred as Karpatiosorbus or Aria species based on the latest taxonomic revision of Sorbus                s.l.  Unfortunately, these endemic species are overlooked and we know nothing about  their                   2                content compounds and biological activities so far. Therefore,  this study  is focused on  the                comparison of the phytochemical profile of Czech Sorbus endemics with emphasis on their α-                glucosidase inhibitory activity. Fruits collected in the Dendrological Garden, Průhonice were                extracted with ethanol, and crude extracts were further partitioned with n-butanol and water to                remove  carbohydrates  and  other  polar  compounds.  n-Butanol  fractions  were  analyzed  by                                                1                HPLC-DAD, HPLC-ELSD, and  H NMR to compare their phytochemical profile and to reveal                the compounds responsible for α-glucosidase inhibitory activity because n-butanol fractions of                all  evaluated  Sorbus  s.l.  species  displayed  greater  inhibition  of  α-glucosidase  than  that  of                acarbose used as a positive control. Results of phytochemical profiling revealed triterpenes as                dominant compounds in n-butanol fractions of all evaluated Sorbus s.l. species and therefore                very probably responsible for bioactivity.                 Acknowledgements: Authors thank Ing. Jiří Velebil and Ing. Martin Lepší for their introduction                to the identification of Czech Sorbus endemics. We would also like to express deep thanks to                Ing.  Jiří  Velebil  for  his  permission  to  collect  Sorbus  fruits  in  the  Dendrological  Garden,                Průhonice.                 References:                1.     Sołtys, A.; Galanty, A.; Podolak, I. Ethnopharmacologically Important but Underestimated Genus Sorbus:                A Comprehensive Review. Phytochem Rev 2020, 19 (2), 491–526. https://doi.org/10.1007/s11101-020-09674-9.                2.     Sennikov, A. N.; Kurtto, A. A Phylogenetic Checklist of Sorbus s.l. (Rosaceae) in Europe. 2017, 93, 1–                78.                                                                                                                      117","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO43                         Screening of Glycyrrhiza species for anti-inflammatory phenols                                         Juraj Markuš *, Karel Šmejkal , Jakub Treml                                                    1,                                                                                   2                                                                     1                  1  Department of Natural Drugs,  Department of Molecular Pharmacy, Faculty of Pharmacy,                                                2                                                    Masaryk University                                        Palackého tř. 1, 612 00 Brno, Czech Republic                                        *Corresponding author: 528110@mail.muni.cz                 Roots and rhizomes of  Glycyrrhiza species (Fabaceae) have  long been used for their  anti-                                                                   1,2                inflammatory, expectorant, and antimicrobial effects.  Our research is dedicated to screening                and selection of different Glycyrrhiza taxa for further isolation of bioactive compounds with                with  an  emphasis  on  prenylated  phenols.  Plant  material  was  obtained  by  cooperation  with                Mendel University, Faculty of Horticulture. Raw material from total of thirteen Glycyrrhiza                varieties  including  six  species  (G.  glabra,  G.  uralensis,  G.  pallidiflora,  G.  foetida,  G.                yunnanensis, and G. echinata) was extracted with ethanol. Extracts were fractionated by liquid-                liquid  extraction  (n-hexane,  chloroform,  ethyl  acetate  and  water). All  fractions  were  then                analysed by HPLC-DAD and ELSD. Chloroform and ethyl acetate fractions were tested for                cytotoxicity and inhibition of NFκB on THP-1 cells. The major compounds from G. uralensis                were isolated by flash chromatography and purified by semipreparative HPLC. HPLC-DAD                analysis  has  not  revealed  any  apparent  intraspecific  difference  in  content  of  phenolic                compounds. Structures of isolated compounds will be elucidated by various spectral methods.                Identified compounds will undergo further bioassays for anti-inflammatory effects.                 Acknowledgements: Authors  thank  to  GAČR,  No.  GA23-04655S  and  Masaryk  University,                Grant No. MUNI/A/1414/2023, Screening and selection of Glycyrrhiza species taxa for anti-                inflammatory prenylated phenols.                 References:                1.     Mahrous,  R.  Metabolic  Bioprofiling  of  Different  Glycyrrhiza  Glabra  Solvent  Fractions  for  the                Identification of Anti-Adenoviral Compounds Using LC-HRMS/MS and in-Vitro Cytopathic Assay Coupled with                Chemometry. BMC Complement. Med. Ther. 2023, 23 (1), 259. https://doi.org/10.1186/s12906-023-04063-z.                2.     Zhao,  L.  Prenylated  Phenolic  Compounds  from  Licorice  (Glycyrrhiza  Uralensis)  and  Their  Anti-                Inflammatory   Activity   against   Osteoarthritis.   Food   Funct.   2022,   13   (2),   795–805.                https://doi.org/10.1039/D1FO03659A.                                                                                                                           118","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO44                   Phlorotannins from brown algae Ecklonia radiata (C.Agardh) J.Agardh –                        isolation, structural elucidation, and antiproliferative properties                                                                              1                     Lenka Molčanová *, Tereza Kauerová , Simona Kapalová , Ludmila Vojtová , Elena                                                                                               1                                                          2                                      1,                                                                                       1                                                                       2                                                         3                                            1                                   Alvarado , Petr Maršík , Peter Kollár , Karel Šmejkal                                                  2                  1  Department of Natural Drugs,  Department of Pharmacology and Toxicology, Faculty of                     Pharmacy, Masaryk University, Palackého tř. 1946/1, 612 00 Brno, Czech Republic                3  Department of Food Quality and Safety, Czech University of Life Sciences Prague, Kamýcká                                       129, 165 00 Prague 6–Suchdol, Czech Republic                                     *Corresponding author: molcanoval@pharm.muni.cz                 Ecklonia radiata (C.Agardh) J.Agardh (Lessoniaceae) is a brown algae found abundantly in the                                                                                   1                warm-temperate  parts  of  South Africa, Australia,  and  New  Zealand .  It  is  a  rich  source  of                phlorotannins,  which  are  polyphenolic  compounds  formed  through  the  polymerization  of                phloroglucinol (1,3,5-trihydroxybenzene). The carbon to carbon and carbon to oxygen linkages                of phloroglucinol units form various types of phlorotannins, such as phloroeckols, phloroethols,                fucols,  and  fucophlorethols.  Phlorotannins  possess  several  bioactivities,  including  anti-                herbivory,  antioxidant,  anti-inflammatory,  antimicrobial,  antiproliferative,  antidiabetic,                radioprotective, adipogenic, antiallergic, and antiviral, demonstrating promising potential in                                                                            2                areas such as food, nutraceutical, and pharmaceutical industry .                The dried water-ethanolic extract of different parts of E. radiata was separated by liquid-liquid                extraction. Ethyl acetate portion was subsequently separated using column chromatography and                semi-preparative RP-HPLC. Identification of the isolated compounds was carried out using UV                and  IR  spectroscopy,  HRMS,  and  NMR  spectroscopy. The  antiproliferative  activity  of  the                isolated compounds was tested in cancer cell lines THP-1, MCF-7, and DU-145 using WST-1                analysis, and subsequently, cell cycle analysis was performed.                 Acknowledgements: Authors  thank  to  Masaryk  University,  Specific  research  –  support  for                student  projects,  Grant  No.  MUNI/ A/1688/2020,  Isolation  of  secondary  metabolites  with                potential biological activity from plant sources, Dr. Kannan R.R. Rengasamy for providing the                plant material, and Susanna Vogliardi, PhD for HRMS data.                 References:                1.     Shrestha, S.; Zhang, W.; Begbie, A.J.; Pukala, T.L.; Smid, S.D. Ecklonia radiata extract containing eckol                protects neuronal cells against Aβ 1–42 evoked toxicity and reduces aggregate density. Food Funct. 2020, 11, 6509–                6516. https://doi.org/10.1039/D0FO01438A                2.     Okeke,  E.S.;  Nweze,  E.J.;  Chibuogwu,  C.C.; Anaduaka,  E.G.;  Chukwudozie,  K.I.;  Ezeorba,  T.P.C.                Aquatic Phlorotannins and Human Health: Bioavailability, Toxicity, and Future Prospects. Nat. Prod. Commun.                2021, 16(12), 1–23. https://doi.org/10.1177/1934578x211056144                                                                                                                 119","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO45                       Agrostemma githago as a rich source of flavonoid-C-glycosides and                                                   triterpene saponins                                                    1                                                                         1,                             Izabela Nawrot-Hadzik , Aleksander Smakosz *, Adam Matkowski     1                  1  Department of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw                                        Medical University, 50-556 Wroclaw, Poland                                 *Corresponding author: izabela.nawrot-hadzik@umw.edu.pl                  In the past Agrostemma githago (corn-cockle) occurred in large quantities in Eurasian fields.                The seeds of this plant ripen at the same time as cereal seeds, so they were collected together                and then ground into flour. Due to the toxicity of Agrostemma githago seeds, poisoning has                occurred after eating flour products containing corn-cockle seeds. It is now known that mainly                ribosome-inactivating proteins (RIPs) are responsible for seeds toxicity. The development of                techniques for cleaning seeds before sowing has resulted in a radical decrease in the presence                of Agrostemma githago. In 2016, it was placed on the Polish red list in the NT (near threatened)                category. Despite the toxicity of the seeds, which caused Agrostemma githago to disappear from                the natural environment, it is known from historical data that the root and herb of this species                were used in folk medicine in the past. In Traditional Chinese Medicine (TCM) the root of A.                githago was known as Mai Xian Weng (麦仙翁). Due to limited data on the phytochemistry of                the herb (data limited to the saponins) and root of A. githago (no data in scientific databases),                detailed phytochemical analyzes were performed.                 Detailed phytochemical analysis (HPLC-DAD-MS, NMR) of extracts, fractions and isolated                compounds  revealed  that  the  Agrostemma  githago  herb  is  a  rich  source  of  flavonoid-C-                glycosides and triterpene saponins, while the root  is a saponin raw  material. Flavonoid-C-                glycosides  have  a  wide  spectrum  of  bioactivity,  including  antioxidant,  anti-inflammatory,                antidiabetic,  anticancer,  hepatoprotective,  antiviral,  antibacterial,  antinociceptive  and                immunomodulatory, which puts Agrostemma githago herb in a new light. The high content of                biologically active compounds in raw materials may justify their traditional use in folk medicine                and encourages further research to determine the effect on the human body.                   Acknowledgements: This research is funded by National Science Center of Poland (NCN) #                2020/39/I/NZ7/01515 (RIPSAPO).                                                                                                                     120","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO46                       Determination of PPAR gamma agonistic activity of mimulone and                                       diplacone in nanoparticle formulations                                                                                                          1                                                                                        3                                                           2                                      1,                                                                          3                 Daniela Nykodýmová *, Lenka Molčanová , Jan Kotouček , Josef Mašek  and Jakub Treml                                                       2                 1  Department of Molecular Pharmacy,  Department of Natural Drugs, Faculty of Pharmacy,                           Masaryk University, Palackého tř. 1946/1, 612 00 Brno, Czech Republic                        3  Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic                                    *Corresponding author: nykodymovad@pharm.muni.cz                 Geranylated  flavonoids  from  Paulownia  tomentosa  have  previously  displayed  potential                                                                                                     1                antidiabetic activity via inhibition of protein tyrosine phosphatase 1B and α-glucosidase.                This study aimed to determine whether these substances can also affect glucose metabolism via                activation of the PPARγ pathway in vitro, to compare the data for the compounds alone and the                same compounds in different nanoparticles, and to determine the most effective nanoparticle                delivery system with the least possible loss of biological effect of the active substance after                encapsulation.                The geranylated flavanones tested, mimulone and  diplacone, were isolated from the unripe                                                                              2                fruits of Paulownia tomentosa (Thunb.) Steud. (Paulowniaceae).                First, the effect of the compounds on the viability of the PPARγ2 CALUX® (U-2 OS) cell line                (BioDetection  Systems  BV,  Netherlands)  was  measured  using  WST-1  assay  kit  (Roche,                Switzerland). Nanoparticle formulations were prepared using the lipid film hydration technique                and detergent methods. Particles of a specific size, concentration, and surface charge were                prepared using Dynamic Light Scattering and MADLS (Multiangle Dynamic Light Scattering).                After 24 hours of incubation, their ability to induce PPARγ-mediated luciferase expression was                                                                         3                assessed using the PPARγ2 CALUX® reporter gene assay.                Mimulone  (2.5μM)  showed  partial  PPARγ-agonistic  activity  (3.9-fold,  p≤0.0001)  as  the                compound itself (the maximum response compared to rosiglitazone at conc. of 2.5 μM was                16.8%). Cyclodextrins proved to be the most effective nanoparticles (with a loss of 10.5% of                mimulone biological activity), the anionic liposome formulations were also active but with a                loss of 46% of mimulone activity. Diplacone showed no statistically significant effect, either as                a substance itself or as a component of nanoparticles.                 Acknowledgements: The authors thank Masaryk University for supporting this project through                Specific Research Programme – Support for Student Projects, Grant No. MUNI/A/1280/2021,                Determination of PPAR gamma agonism of selected natural products in nanoparticle systems.                 References:                1.     Song, Y. H.et al. Inhibition of Protein Tyrosine Phosphatase (PTP1B) and α-Glucosidase by Geranylated                Flavonoids  from  Paulownia  Tomentosa. J.  Enzyme  Inhib.  Med.  Chem.   2017, 32(1),  1195–1202.                https://doi.org/10.1080/14756366.2017.1368502                2.     Šmejkal, K. et al. C-Geranyl Compounds from Paulownia Tomentosa Fruits. J. Nat. Prod. 2007, 70(8),                1244-1248. https://doi.org/10.1021/np070063w                3.     Gijsbers, L. et al. Induction of Peroxisome Proliferator-Activated Receptor γ (PPARγ)-Mediated Gene                Expression by Tomato (Solanum Lycopersicum L.)  Extracts. J. Agric. Food Chem. 2013, 61(14), 3419–3427.                https://doi.org/10.1021/jf304790a                                                                                                             121","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                47                      Formulation with three herbs exhibits antiviral activity against highly                                      pathogenic influenza a virus in chickens                                                                                            2                                              1,                                                                            1                                                              1                                Won Keun Oh *, Eun Jin Park , Ba Wool Lee , Eunhee Kim                 1  Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul                                                 08826, Republic of Korea                  2  Choong-Ang Vaccine Laboratory, 1476-37, Yuseong-daero, Yuseong-gu, Daejeon 34055,                                                     Republic of Korea                                         *Corresponding author: wkoh1@snu.ac.kr                  Influenza,  particularly  caused  by  Influenza A  viruses  (IAVs),  poses significant  health  risks                worldwide, affecting vulnerable populations such as young children, pregnant women, and the                elderly. Despite advancements in medical technology and the development of antivirals, the                fight against flu continues, highlighted by the 2009 H1N1 pandemic resulting in up to 575,400                deaths in the U.S. alone. Traditional methods to combat IAVs, including antiviral medications                and  vaccinations,  face  challenges  due  to  the  virus's  rapid  evolution,  leading  to  resistance.                Moreover, zoonotic avian influenza viruses (AIVs) present new public health risks, with control                measures  like  mass  culling  causing  economic  and  ethical  concerns.  This  study  explores  a                multicomponent and multitarget (MCMT) approach, derived from traditional Chinese medicine                (TCM),  which  combines  different  medicinal  plants  to  lower  the  chance  of  resistance                             1                development .                We  investigated  the  antiviral  effects  of  three  herbal  formulation  consisting  of  Angelica                dahurica, Pinus densiflora, and Curcuma longa, known for their respective antiviral properties,                against  IAV  H1N1 .  Our  findings  show  promising  results,  particularly  for  a  selected                                    2,3                combination enhanced with ascorbic acid and chitosan, demonstrating significant anti-influenza                activity in chickens infected with the highly pathogenic avian influenza (HPAI) A H5N6. This                study suggests the potential of MCMT strategies in developing effective antiviral treatments                against IAVs and AIVs, offering a novel approach to addressing current limitations in influenza                management.                 Acknowledgements: This research was supported by grants from the Basic Science Research                Program (NRF-2022R1A2C2005061) and (RS-2023-00218616) through the National Research                Foundation of Korea (NRF) funded by the Ministry of Science and ICT in Korea.                 References:                1.     Park, E. J.; Lee, B. W.; Ryu, B.; Cho, H. M.; Kim, S. K.; Yoo, S. S.; Kim, W.; Oh, W. K. Antiviral activity                of CAVAC-1901, a combination of three standardized medicinal plants, against highly pathogenic influenza A                virus in chickens. Poult Sci.  2023, 102, doi: 10.1016/j.psj.2022.102315.                2.     Lee, B. W.; Ha, T. K. Q.; Cho, H. M.; An, J. P.; Kim, S. K.; Kim, C. S.; Kim, E.; Oh, W. K. Antiviral                activity of furanocoumarins isolated from  Angelica dahurica  against influenza a viruses H1N1 and H9N2.  J                Ethnopharmacol. 2020, 259, doi: 10.1016/j.jep.2020.112945.                3.     Ha, T. K. Q.; Lee, B. W.; Nguyen, N. H.; Cho, H. M.; Venkatesan, T.; Doan, T. P.; Kim, W.; Oh, W. K.                Antiviral  activities  of  compounds  isolated  from Pinus  densiflora (Pine  Tree)  against  the  Influenza  A                Virus. Biomolecules.  2020, 10, 576-666. doi: 10.3390/biom10050711.                                                                                                              122","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO48                        Evaluation of reference gene expression stability by quantitative                                       real-time PCR in Agrostemma githago                                                                            1                                                                                                 1                                                          1                 Bartosz Pencakowski *, Monika Bielecka , Marta Stafiniak , Weronika Kozłowska , Izabela                                      1,                                       1                                                          2                                                                               2                                                                                                  1                        Nawrot-Hadzik , Katarzyna Nowis , Łukasz Łaczmański , Adam Matkowski                   1  Department of Pharmaceutical Biology and Biotechnology, Wroclaw Medical University,                                                     Wroclaw, Poland                      2  Laboratory of Genomics \& Bioinformatics, Hirszfeld Institute of Immunology and                                        Experimental Therapy PAS, Wroclaw, Poland                                  *Corresponding author: bartosz.pencakowski@umw.edu.pl                 Quantitative real-time polymerase chain reaction (qPCR) is widely used for gene expression                studies, with high specificity, sensitivity and repeatability. However, for precise and reliable                expression analysis using the relative quantification approach, a selection of a stable reference                gene is a prerequisite to accurately normalize the data. Expression of internal reference genes                can  vary  considerably  during  developmental  stages  and  under  different  experimental                          1                conditions .                For  Agrostemma  githago  L.  (corn  cockle,  Caryophyllaceae),  there  are  no  reports  of  stable                reference genes. In order to study the expression of genes related to natural product biosynthesis                and regulation of specialized metabolism, we aimed at selecting most stable reference genes                across all studied plant tissues and growing conditions.                                           2,3                Based  on  previous  studies ,  10  frequently-used  housekeeping  genes:  actin,  translation                elongation  factor  1,  glyceraldehyde-3-phosphate  dehydrogenase,  tubulin  α,  tubulin  β,                ubiquitin,  histone  H3,  elongation  factor  1-α,  elongation  factor  1-β,  eukaryotic  translation                initiation factor 5A, and serine/threonine protein phosphatase 2A were selected for evaluation                as  potential  reference  genes  in  A.  githago.  The  integrated  expression  stability  of  potential                reference genes was established with the RefFinder tool (https://blooge.cn/RefFinder/), which                integrates following computational statistical methods: the geNorm, NormFinder, BestKeeper,                and the comparative Δ-Ct method. The results showed that the expression stability of candidate                genes  varies  in  different  sample  pools,  but  allowed  for  successful  selection  of  three  most                adequate reference genes.                 Acknowledgements: The study was supported by the Wroclaw Medical University, grant No.                SUBZ.D030.24.081                 References:                1.     Czechowski, T.; Stitt, M.; Altmann, T.; Udvardi, M. K.; Scheible, W. R. Genome-Wide Identification and                Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis. Plant Physiol. 2005, 139, 5–17.                https://doi.org/10.1104/pp.105.063743.                2.     Rodríguez-Parra, A.; Picazo-Aragonés, J.; Balao, F. Evaluation of Reference Genes in the Polyploid                Complex     Dianthus   Broteri   (Caryophyllaceae)   Using   QPCR.     Plants   2022,    11.                https://doi.org/10.3390/plants11040518.                3.     Yu, W.; Tao, Y.; Luo, L.; Hrovat, J.; Xue, A.; Luo, H. Evaluation of Housekeeping Gene Expression                Stability  in  Carnation  (Dianthus  caryophyllus).  New  Zeal.  J.  Crop  Hortic.  Sci.  2021,  49,  347–360.                https://doi.org/10.1080/01140671.2021.1883069.                                                                                                               123","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO49                           Synthesis and screening of carltonine mimetics for selective                                           butyrylcholinesterase inhibition                                                                                         3                                                                       3                             1,                                                   2                 Filip Pidaný *, Xavier Brazzolotto , Martina Hrabinová , Jan Korábečný , Lucie Cahlíková 1                 1  Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy                  and Pharmaceutical Botany, Akademika Heyrovského 1203, 500 05 Hradec Králové, Czech                                                         Republic                    2  Institut de Recherche Biomédicale des Armées, Départment de Toxicologie et Risques                        Chmiques, 1 Place Général Valérie André, 91220, Brétigny-sur-Orge, France                  3  University Hospital Hradec Králové, Biomedical Research Center, Sokolská 581, 500 05                                              Hradec Králové, Czech Republic                                         *Corresponding author: pidanyf@faf.cuni.cz                 Butyrylcholinesterase (BChE) assumes a pivotal role in the pathophysiology and progression                of Alzheimer's disease (AD). As AD progresses, acetylcholinesterase levels decrease by over                90%,  while  BChE  levels  rise  to  165%  of  their  normal  level,  suggesting  a  compensatory                mechanism.  We  are  exploring  innovative  Alzheimer's  drug  candidates,  focusing  on                Amaryllidaceae alkaloids carltonine A and B. Our research group has previously isolated these                                                                                          1                compounds, revealing their significant selectivity for human BChE (hBChE) .                This study encompasses the design, synthesis, and in vitro evaluation of compounds exhibiting                hBChE inhibitory potential, ranging from micromolar to low nanomolar scale. Compounds                demonstrating  the  most  significant  inhibition  underwent  theoretical  validation  for  their                potential to penetrate the CNS, using the BBB score algorithm, corroborated by in vitro PAMPA                results. Additionally,  the  safety  profiles  of  these  compounds  were  assessed  using  human                neuroblastoma and hepatocellular carcinoma cell lines. Significantly, compounds 87 (hBChE                IC50 = 3.8 ± 0.2 nM) and 88 (hBChE IC50 = 5.7 ± 1.5 nM) have emerged as exemplary inhibitors.                A  crystallographic  study  was  conducted  to  elucidate  the  binding  mode  of  the  most  potent                inhibitor. Distinguished by its novel scaffold, compound 87 emerges as a promising lead for                tackling mid to late-stage AD challenges .                                                       2                 Acknowledgements: The study was supported by Charles University SVV 260 662.                 References:                1.     Xing,  S.;  Li,  O.;  Xiong,  B.;  Chen, Y.;  Feng,  F.;  Liu, W.;  Sun,  H.  Structure  and  therapeutic  uses  of                butyrylcholinesterase:  application  in  detoxification,  Alzheimer's  disease,  and  fat  metabolism.  Med.  Res.                Rev. 2021, 41, 858-901. https://doi.org/10.1002/med.21745                2.     Pidany, F.; Kroustkova, J.; Mamun, A. A.; Suchankova, D.; Brazzolotto, X.; Nachon, F.; Chantegreil, F.;                Dolezal, R.; Pulkrabkova, L.; Muckova, L.; et al. Highly selective butyrylcholinesterase inhibitors related to                Amaryllidaceae alkaloids - design, synthesis, and biological evaluation. Eur. J. Med. Chem. 2023, 252, 115301.                https://doi.org/10.1016/j.ejmech.2023.115301                                                                                                                 124","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO50                      Colon-targeted delivery of pomegranate and blackcurrant seed oils                                            exhibiting antitumor activity                                           1                  Joanna Wojciechowska , Magdalena Grajzer , Aleksandra Dyba , Benita Wiatrak , Karol                                                             1                                                                                2,3                                                                                                 4                                                                         1                                                          2                                                Nartowski , Anna Prescha *                  1 Department of Dietetics and Bromatology, Wroclaw Medical University (WMU), Borowska                                               211, 50-556 Wroclaw, Poland;                   2                     Department of Drug Form Technology, WMU, Borowska 211, 50-556 Wroclaw, Poland;                    3                      Institute of Pharmacy, University of Innsbruck, Innrain 52c, 6020 Innsbruck, Austria;                   4 Department of Pharmacology, WMU, Mikulicza Radeckiego 2, 50-345 Wrocław, Poland                                     *Corresponding author: anna.prescha@umw.edu.pl                 Components of vegetable oils, including polyunsaturated fatty acids (PUFAs) and sterols, can                inhibit colorectal cancer cells. Pomegranate seed (PSO) and black currant seed (BCSO), laden                with various PUFAs, sterols and tocopherols with antitumor activity, are good candidates for                preventive and supportive anticancer agents in chemotherapy. Encapsulation of oils using cross-                linking with alginate and chitosan may enable colon delivery of unaffected oil components and                extend their retention time in the colon due to mucosal adhesion of chitosan.                We aimed to investigate the composition and in vitro anticancer potential of pomegranate (PG)                and blackcurrant (BC) seed oils extracted by cold-pressing, and to test the oxidative stability                and release under simulated gastrointestinal conditions of oils encapsulated by cross-linking                with alginate and chitosan or its oleoyl-derivative.                The GC analyses of fatty acids and sterols, and UPLC analyses of tocopherols and carotenoids                were performed. Oil stability was assessed using PV, AV and DSC. Chemopreventive potential                of 1 – 5% oil-in-water emulsions against LoVo, LoVo/DX and CCD 841 CoTr cell lines was                investigated  using  MTT  assay,  fluorescence  microscopy  of  the  apoptotic  cell  number,                Rhodamine-123 test and cellular antioxidant analysis (CAA).                PG and BC oils were encapsulated by drip feeding into a CaCl2 solution the alginate based                emulsion (5% Na alginate and 20% oil) with further chitosan/oleoyl chitosan coating. The                effectiveness of encapsulation was verified using FTIR and NMR.                BC was characterized by high tocopherol (1649 mg/kg) and carotenoid (483 mg/kg) amounts                and higher increase in apoptotic cells and Rhodamine-123 accumulation than in case of PG.                High punicic acid (63%) and phytosterol (6427 mg/kg) contents in PG were accompanied by a                stronger  influence  on  the  cytotoxic  effect  of  doxorubicin  on  cancer  cells.  Encapsulation                significantly  enhanced  PG  and  BC  oxidative  stability  and  enabled  safe  in  vitro  simulated                gastrointestinal transit into the colon.                 Acknowledgements:  Authors  thank  to  Wroclaw  Medical  University,  Grant  No.                SUBZ.D040.23.028  „Składniki  diety  a  stan  zapalny:  stabilność  i  biodostępność  substancji                lipofilnych  o  działaniu  przeciwzapalnym  mikrokapsułkowanych  z  użyciem  polimerów                roślinnych”.                                                                                                                     125","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO51                         Quality assessment of Boswellia serrata extract- containing food                                       supplements on the Hungarian market                                            1,                                                              1                                                                           1                                                                                         1,2                                Péter Püski *, Panna Pribelszki , Anita Barta , Tivadar Kiss                   1  Institute of Pharmacognosy, University of Szeged, H-6720 Szeged, Eötvös u. 6., Hungary                   2  HUN-REN-USz Biologically Active Natural Products Research Group, Szeged, H-6720                                               Szeged, Eötvös u. 6. Hungary                                       *Corresponding author: peterpuski@gmail.com                                                                                                          1                Boswellia serrata extract is applied in the management of osteoarthritis as a food supplement .                The quality of food supplements is scarcely monitored in Hungary. Moreover, the limited access                to the drug is a red flag in terms of product quality. The European Pharmacopeia’s quality                parameters are based on the content of marker compounds in drug (AKβBA: acetyl-11-keto-β-                boswellic  acid  and  KβBA:  11-keto-β-boswellic  acid);  whereas,  commercial  extracts  are                characterized by their total boswellic acid content (TBA). The latter parameter is not specific                                                                        2                enough to ensure the possible adulteration of the products . The AKβBA and KβBA content of                                                                 2                the drug should be not less than 1%, respectively . For extracts EFSA recommends 2.53%-                3.68% of AKβBA and 2.35%–3.57% of KβBA content. The TBA should not be lower than 65%                determined by titrimetry .                                        3                In  our  research  seven  frankincense  containing  products  were  purchased  in  Hungarian                pharmacies. The quality of the obtained products was screened applying various instrumental                analytical methods (HPLC-PDA, LC-MS,  H-NMR). Four major boswellic acids (AKβBA,                                                          1                KβBA, AβBA: acetyl-β-boswellic acid, βBA: β-boswellic acid) were quantified, as well as the                1 H-NMR fingerprint spectra of the products were compared to a USP grade reference extract.                Result of the chromatographic measurements was interpreted based on the USP-NF. The sum                of  KBA  and AKBA  was  calculated  based  on  the  labelled  amount  of  extract  in  the  food                supplements. In four out of seven products miniscule amount of boswellic acids (the sum of                AkβBA and KβBA was 0.00%–0.30%) as well as the lack of characteristic  H-NMR fingerprint                                                                                       1                were  observed.  No  quality  issues  had  been  risen  in  three  product  based  on  boswellic  acid                content; whereas, in the suitable food supplements, similar boswellic acid profile was observed:                AKβBA: 5.88%–7.07%; KβBA: 3.09%–4.12%; βBA: 10.38%–22.42%; AβBA: 3.49%–8,87%.                 References:                1.     Abdel-Tawab, M.; Werz, O.; Schubert-Zsilavecz, M. Boswellia Serrata: An Overall Assessment of in                Vitro,  Preclinical,  Pharmacokinetic  and  Clinical  Data.  Clin  Pharmacokinet  2011,  50  (6),  349–369.                https://doi.org/10.2165/11586800-000000000-00000.                2.     Indian  frankincense  -  European  Pharmacopoeia  11.2.  https://pheur.edqm.eu/app/11-2/content/11-                2/2310E.htm?highlight=on\&terms=frankincense (accessed 2023-03-08).                3.     EFSA  Panel  on Additives  and  Products  or  Substances  used in Animal  Feed  (FEEDAP).  Safety  and                Efficacy of a Feed Additive Consisting of an Extract of Olibanum from Boswellia Serrata Roxb. Ex Colebr. for                Use   in   Dogs   and   Horses   (FEFANA   Asbl).   EFSA   Journal   2022,   20   (3),   7158.                https://doi.org/10.2903/j.efsa.2022.7158.                                                                                                              126","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO52                     2D- NMR chemical investigation of bioactive fractions of Clinopodium                                                           nepeta                                                                                                 1                                       1,                                                                            2                                                           1                          Carlo Raucci *, Francesca Guzzo , Gokhan Zengin , Brigida D’Abrosca                   1  Department of Environmental Biological and Pharmaceutical Sciences and Technologies,                   DiSTABiF University of Campania Luigi Vanvitelli, Via Vivaldi 43, 81100 Caserta, Italy                      2 Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey                                    *Corresponding author: carlo.raucci@unicampania.it                 Clinopodium nepeta subsp. glandulosum belongs to the Lamiaceae family is known to be a                polymorphic  and  fragrant  plant  with  traditional  uses  including  medicinal  herbs  for  anti-                spasmodic,  diuretic,  stimulant,  and  tonic  purposes  (Debbabi  et  al.,  2020).  Several  studies                regarding essential oils have been revealed a high content of oxygenated monoterpenes, such                as  pulegone,  piperitenone  and  piperitenone  oxide  (Boškailo  et  al.,  2022)  as  well  as  their                antimicrobial,  anti-candida,  antioxidant,  and  insecticidal  activities  (Debbabi  et  al.,  2020).                Despite several investigations regarding essential oils and their biological activities, at the best                of our knowledge, still little is known about chemical composition in term of polar compounds                present  in  crude  extract.  Based  on  these  considerations,  the  aim  of  the  present  study  is  to                investigate the polar extracts of C. nepeta subsp. glandulosum in term of metabolic composition                and  biological  activities.  The  current  work  was  addresses  to  investigate  six  extracts  of                Clinopodium nepeta subsp. glandulosum through biological assays and NMR analysis, in order                to furnish information about the specialized metabolite content. The hydroalcoholic extract was                the most promising one in term of antioxidant and enzyme inhibiting activities, reporting a                DPPH radicals inhibition of 48.07 ± 0.21 mg TE/g and a AChE inhibitory capacity of 2.66 mg                GALAE/g, respectively. As highlighted by exhaustive 2D-NMR investigation hydroalcoholic                extract was dominated by resonance of flavonoidic glucosides and triterpenoidic compounds,                whose  fully  characterization  was  reach  only  after  a  liquid-liquid  separation  and                chromatographic  purification.  Through  deeply  2D-NMR  analysis,  acacetin  glycoside                derivatives,  flavonol  glycosides,  ursolic  acid  and  2,4-di-t-butylphenol  were  highlighted  as                principal component of more bioactive extract.                 References:                1.     Boškailo, E. Clinopodium nepeta (L.) Kuntze from Bosnia and Herzegovina: Chemical Characterisation                of  Headspace  and  Essential  Oil  of  Fresh  and  Dried  Samples.  Rec.  Nat.  Prod.  2022,  17:2,  300-311.                http://doi.org/10.25135/rnp.357.2207.2504                2.     Debbabi, H. Chemical Composition, Antifungal and Insecticidal Activities of the Essential Oils from                Tunisian Clinopodium nepeta subsp. nepeta and Clinopodium nepeta subsp. Glandulosum. Molecules. 2020, 25,                2137. https://doi.org/10.3390/molecules25092137                                                                                                                   127","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO53                      Nature versus cultivation: exploring the phytochemical diversity and                            bioactive potential of the halophyte Plantago coronopus L.                                                           2                                        1                                                                                 1,                                                                                                    1                       Alexia dos Santos , Riccardo Trentin , Maria João Rodrigues *, Luísa Custódio                  1  Centre of Marine Sciences (CCMAR/CIMAR LA), University of Algarve, Faculty of Sciences                and Technology, Ed. 7, Campus of Gambelas, University of Algarve, 8005-139 Faro, Portugal                    2  Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy                                        *Corresponding author: mjrodrigues@ualg.pt                 The resilience and nutritional profile of halophyte plants, particularly Plantago coronopus L.,                a member of the Plantaginaceae family, highlight their potential for sustainable agriculture in                saline-prone environments. P. coronopus thrives in the Mediterranean, North Africa, and the                Middle  East,  offering  a  rich  source  of  proteins,  vitamins,  minerals,  and  fibres,  along  with                various  bioactive  compounds  with  promising  therapeutic  applications.  With  the  escalating                interest in halophyte cultivation due to their adaptability to harsh saline conditions, this study                delves into a comparative analysis of PC's bio-properties and chemical variability in nature                versus greenhouse conditions. Methanol extracts were evaluated for their DPPH and ABTS                radical-scavenging activities, copper and iron chelation, ferric-reducing antioxidant power, and                enzymatic inhibitory properties towards AChE, BuChE, α-glucosidase, α-amylase, and lipase.                The extracts were also evaluated for their total phenolics, flavonoids, and condensed tannin                contents, followed by the chemical profiling of bioactive compounds using Ultra-Performance                Liquid Chromatography coupled with High-Resolution Mass Spectrometry/Mass Spectrometry                (UPLC–HR–MS/MS).  Generally,  samples  collected  from  nature  exhibited  higher  radical-                scavenging,  metal-chelation,  ferric-reducing,  and  enzyme-inhibitory  properties,  as  well  as                higher  total  contents  of  phenolics,  flavonoids,  and  condensed  tannins.  Moreover,  chemical                profiling  distinguished  the  metabolomes  of  wild  and  greenhouse  samples.  Out  of  the  331                compound  extract  features,  211  showed  significant  differences  between  the  wild  and                greenhouse  samples.  Library  spectral  matches  allowed  the  annotation  of  23  statistically                significant features. Trehalose, skimmine/isomer, and 13, 16, 19–docosatrienoic acid were more                abundant in greenhouse samples. The remaining twenty features showed a significant increase                in wild extracts of P. coronopus, most of which belonged to cinnamic acids and derivatives and                prenol  lipids. The  implications  of  our  findings  contribute  to  refining  halophyte  cultivation                practices, enhancing sustainable agriculture in arid regions, and investigating P. coronopus as a                viable source of health-beneficial bioactive compounds.                 Acknowledgements: This research was funded by Portuguese national funds from the FCT -                Foundation  for  Science  and  Technology,  through  projects  UIDB/04326/2020,                UIDP/04326/2020, LA/P/0101/2020, and the GreenValue (PTDC/BAAAGR/1391/2020). MJR                benefited from an FCT program contract (UIDP/04326/2020), and LC was supported by the                FCT Scientific Employment Stimulus (CEECIND/00425/2017).                                                                                                             128","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO54                   Isolation and identification of compounds from Schisandra chinensis fruit                  and effect of dibenzocyclooctadiene lignans on gap junction intercellular                                                     communication                                                                   1                                                                                                  1,2                                                  1                                 1,                Michal Rybnikář *, Milan Malaník , Karel Šmejkal , Emil Švandlejka  Polina Shpet , Pavel                                                                                    1,                                                      2                                               Babica , Stefano Dall´Acqua 3                    1  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Brno, Czech                                                         Republic                           2  Recetox, Faculty of Science, Masaryk University, Brno, Czech Republic                 3  Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Italy                                    *Corresponding author: michalrybnikar84@gmail.com                                                                                   1                Schisandra chinensis belongs to 50 basic herbs of Chinese medicine . It is a deciduous, shrub-                like,  dextrorotatory  creeper  without  tendrils.  Dibenzocyclooctadiene  lignans  are  the  most                important active substances from S. chinensis., which have a biological and pharmacological                activity.                An investigation of compounds of the fruit of S. chinensis led to the isolation and structural                determination  of  eight  dibenzocyclooctadiene  lignans  and  arisantetralones A  and  C  never                isolated  from  S.  chinensis  before.  We  isolated  new  sesquiterpene  7,7-dimethyl-11-                methylidenespiro[5.5]undec-2-ene-3-carboxylic     acid.    Gap     junction    intercellular                communication  (GJIC)  allows  communication  between  cells  and  the  exchange  of  small                                                                         2                molecules  through  connexin-based  gap  junction  channels .  Selected  dibenzocyclooctadiene                lignans were tested to evaluate their effect on gap junction intercellular communication in WB-                ras cells. This is the first report on GJIC for dibenzocyclooctadiene lignans.                 References:                1.     Wong, M. La Médicine Chinoise par les Plantes, 1st ed., Le Corps a Vivre Series, Éditions Tchou: Paris,                France, 1976; Volume 274.                2.     Caruso, G.; Di Pietro, L.; Caraci, F. Gap Junction and Connexins in Microglia-Related Oxidative Stress                and Neuroinflammation: Perspectives for drug Discovery. Biomolecules 2023, 13, 505.                3.     Rybnikář, M.; Malaník, M.; Šmejkal, K.; Švajdlenka, E.; Shpet, P.; Babica, P.; Dall’Acqua, S.; Smištík,                O.;  Jurček,  O.; Treml,  J.  Dibenzocyclooctadiene  Lignans  from  Schisandra  chinensis  with Anti-Inflammatory                Effects. Int. J. Mol. Sci. 2024, 25, 3465. https://doi.org/10.3390/ijms25063465                                                                                                                          129","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO55                    Effect of short-term apple consumption on fecal metabolites: A pre-post                                     interventional study using NMR analysis                        Chandrama Roy Chowdhury*, Šárka Knížková, Eliska Jeníčková, Jaroslav Havlik                                          Czech University of Life Sciences, Prague                                     *Correspondingg author: roy_chowdhury@af.czu.cz                 Apples  are  an  important  source  of  dietary  fibre,  vitamins  K,  C,  B6,  and  phytonutrients.                Prolonged apple consumption has demonstrated potential anti-cancerous, anti-diabetic, anti-                obesity, and anti-inflammatory properties, with many of these benefits being attributed to the                                                   1                fruit's phytonutrients or dietary fibre . Nuclear magnetic resonance (NMR) ̶based metabolomics                is a powerful method for capturing a snapshot of 40-60 abundant metabolites in biological                samples.  Meanwhile,  NMR  metabolomics  offers  insights  into  an  individual's  dietary  and                                                                                        2                digestive processes in the colon and the composition of their gut microbiota . Our objective was                to explore the changes in the faecal metabolites associated with increased apple intake. Based                on  existing  research,  we  anticipated  an  increase  in  short-chain  fatty  acids  (SCFAs)  and                metabolic by-products of dietary phenolics, such as phenylacetic acid derivatives.                Fifteen participants submitted stool samples before and after a three-day apple consumption                intervention. These samples were analysed to identify and quantify changes in faecal metabolite                concentrations.  Univariate  analysis  showed  a  moderate  decrease  of  methanol  (FC=0.35,                p=0.016, FDR=0.339), but an increase of propionate (FC=1.19, p=0.029, FDR=0.339), valine                (FC=1.24,  p=0.026,  FDR=0.339),  fumarate  (FC=1.24,  p=0.040,  FDR=0.339),  glycine                (FC=1.18, p=0.047, FDR=0.339), and aspartate (FC=1.20, p=0.045, FDR=0.339) following the                intervention.  These  changes  were  not  statistically  significant  after  adjusting  for  multiple                comparison  corrections.  Interestingly,  methanol  levels  decreased,  which  contrasts  with  the                assumption that pectin fermentation will lead to the opposite effect. The marginally significant                changes in faecal metabolites post-intervention suggest that short-term dietary intake has a                minimal effect on the stool's metabolic profile. The most substantial differences are attributed                to individual variances, which remain constant and do not fluctuate with such short-term dietary                adjustments. Further research is needed to understand the impact of diet on the gut microbiota's                composition.                 Acknowledgements: Authors thank the Grant Agency of Czech Republic GACR 23-04655S                and the Internal grant agency of the CZU - FAFNR (SV22-5-21310).                 References:                1.     Hyun, T.K. and Jang, K.I. Apple as a source of dietary phytonutrients: an update on the potential health                benefits of apple. EXCLI J., 2016, 15, p.565. https://doi.org/10.17179%2Fexcli2016-483                2.     Karu, N., Deng, L., Slae, M., Guo, A.C., Sajed, T., Huynh, H., Wine, E. and Wishart, D.S. A review on                human fecal metabolomics: Methods, applications and the human fecal metabolome database. Anal. chim. acta,                2018, 1030, pp.1-24. https://doi.org/10.1016/j.aca.2018.05.031                                                                                                             130","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO56                      Extraction of cannabinoids, volatile terpenes, and polyphenols from                      Cannabis sativa: subcritical water vs. supercritical CO2 and organic                                                         solvents                                                        1,                                           Katja Schoss *, Nina Kočevar Glavač                                                                                1                    1  University of Ljubljana, Faculty of Pharmacy, Department of Pharmaceutical Biology,                                           Aškerčeva 7, 1000 Ljubljana, Slovenia                                      *Corresponding author: katja.schoss@ffa.uni-lj.si                 The utilization of hemp (Cannabis sativa) has garnered significant attention due to its diverse                                                                                                      3                applications  across  pharmaceuticals,  food,  and  cosmetics  industries.  Employing  a  2   full                factorial Design of Experiments approach, we investigated subcritical water extraction as a                 sustainable  extraction  technique (1)  for  obtaining  hemp  extracts  (var.  'Futura  75')  as  an                alternative to supercritical CO2 extraction (2), ultrasonic extraction (organic solvents, water),                Soxhlet and high-pressure ethanol extraction and hydro-distillation.                In the extraction with subcritical water, we confirmed statistically significant models for the                yield, the antioxidant activity (determined by the DPPH and ABTS tests), and the content of                polyphenolic compounds (determined by the FC test) for the studied factors (temperature in all                models, drug to solvent ratio and product temperature* drug to solvent ratio in the yield). The                highest yield was obtained with subcritical water extraction (35.91%), and temperature and                drug to solvent ratio were the key parameters. Soxhlet extraction (36.06% CBG) and high-                pressure ethanol extraction (17.39% CBGA) proved to be the most suitable for the extraction                of  cannabinoids.  Subcritical  water  extraction  yielded  extracts  with  a  comparably  high                antioxidant activity and polyphenol (temperature was a key parameter) content as the extracts                obtained with other methods.                Essential oil and condensed Clevenger water fraction were found to be significantly different,                the former containing predominantly sesquiterpenes (82.51%) and oxygenated sesquiterpenes                (9.12%),  while  the  latter  was  rich  in  oxygenated  monoterpenes  (34.97%)  and  non-terpene                compounds with carbonyl groups (45.16%).                In conclusion, subcritical water extraction presents a promising method for obtaining novel                hemp extracts with relatively high antioxidant activity and favorable yield.                 Acknowledgements: This research was funded by the Slovenian research agency grant numbers                P4-0127; MR-54785.                 References:                1.     Chakraborty  S,  Shaik  L,  Gokhale  JS.  Subcritical  Water: An  Innovative  Processing  Technology.  In:                Innovative Food Processing Technologies. Elsevier; 2021. p. 552–66.                2.     Jokić  S,  Jerković  I,  Pavić V, Aladić  K,  Molnar M,  Kovač MJ,  et al. Terpenes and  Cannabinoids  in                Supercritical  CO2  Extracts  of  Industrial  Hemp  Inflorescences:  Optimization  of  Extraction,  Antiradical  and                Antibacterial Activity. Pharmaceuticals. 2022 Sep 1;15(9):1117.                                                                                                              131","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO57                  Antimicrobial properties of phenolics against fusidic acid-resistant MRSA                                                    in wound healing                                                                                      3                                                                                                      2                                                                1                    Gabriela Škovranová  1,2, *, Monika Trembecká , Helena Žemličková , Alice Sychrová                  1  Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Palackého                                              tř. 1946/1, 612 00 Brno, Czechia                   2  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého tř.                                               1946/1, 612 00 Brno, Czechia                  3  Centre of Epidemiology and Microbiology, National Institute of Public Health, Šrobárova                                               48, 100 00 Vinohrady, Czechia                                    *Corresponding author: skovranovag@pharm.muni.cz                 Staphylococcus  aureus  is  a  pathogen  responsible  for  skin  and  soft-tissue  infections.  The                emergence  of  methicillin-resistant  S.  aureus  (MRSA)  leads  to  antibiotic  treatment  failure,                including topical antibiotics. Plant-derived natural compounds, especially prenylated phenolics,                                                                         1                provide a significant source of potent antimicrobial agents . Firstly, the antimicrobial activity                of eight compounds was evaluated against six MRSA clinical isolates resistant to fusidic acid.                The minimum inhibitory concentrations (MICs) were assessed using the microdilution method.                The  synergistic  potential  with  fusidic  acid  was  measured  by  the  checkerboard  titration                technique,  and  the  fractional  inhibitory  concentration  (FIC)  described  synergy  (FIC  ≤0.5),                additive effect (0.5< FIC ≤1); and indifference (1< FIC ≤4). Prenylated phenolics inhibited the                growth of MRSA in a range of 4–16 μg/mL. MIC of fusidic acid proved resistance of all clinical                isolates (MIC ≥2 μg/mL). Kuwanon E, U, morusin, and Diels-Alder adducts kuwanon G and                albafuran  C  in  combination  showed  additive  effects  against  at  least  one  MRSA  isolate.                Kuwanon  C  with  the  antibiotic  revealed  additive  effect  against  five  MRSA  strains  and                represents the most effective combination. Thymol and epigallocatechin gallate showed higher                MIC (≥32 μg/mL) but, in combination with fusidic acid, revealed partial synergism against at                least  three  MRSA  strains.  No  synergism  was  observed  for  any  combinations  of  selected                compounds  with  fusidic  acid.  To  sum  up,  prenylated  phenolics  represent  prospective                antimicrobial agents against MRSA, and their anti-inflammatory and antioxidant effects could                further support wound healing.                 References:                1      Sychrová, A.; Škovranová, G.; Čulenová, M.; Bittner Fialová, S. Prenylated Flavonoids in Topical                Infections and Wound Healing. Molecules 2022, 27 (14), 4491. https://doi.org/10.3390/molecules27144491.                                                                                                                       132","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO58                      Agrostemma githago (common corn-cockle) seeds as a new source of                                                 unsaturated fatty acids                                                  1,                                                                                              1                            Aleksander Smakosz *, Izabela Nawrot-Hadzik , Adam Matkowski                                                                           1                  1  Department of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw                                        Medical University, 50-556 Wroclaw, Poland                               *Corresponding author: aleksander.smakosz@student.umw.edu.pl                 Agrostemma githago L. (corn-cockle) is a segetal weed, which in the past occurred in large                quantities in Eurasian fields. It was regarded as a significant toxicological agent, due the high                ribosome-inactivating proteins (RIPs) and saponins content. To date, most studies on this taxon                have focused on above groups of specialized metabolites.                Among neglected constituents of A. githago fatty acids may be listed. Our studies show that                seeds of corn-cockle could be used as a novel source of oil for polyunsaturated fatty acids.                The  oil  was  isolated  from  seeds,  using  hexane  as  a  solvent.  In  the  next  step,  the                transesterification was performed. The compositions of the seed oils and essential oils were                determined using a gas chromatography–mass spectrometry (GC–MS) instrument.                The most important constituents, found in tested sample are: benzyl alcohol, dodecyl acrylate,                14-methyl-pentadecanoic acid methyl ester, 9,12-Octadecadienoic acid (Z,Z) methyl ester, and                9,12,15-octadecatrienoic acid methyl ester.                Further analysis and optimalisation of isolation process could help to utilize oil of A. githago                in cosmetic, food and pharmaceutical industries.                 Acknowledgements:  Authors  thank  to  Wroclaw  Medical  University,  SIMPLE:                SUBK.D030.24.036                                                                                                                                        133","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO59                     Anti-inflammatory and antioxidant activity of dibenzocyclooctadiene                                         lignans from Schisandra chinensis                                                                                     2                                                                    2                                                                                                         3                                                   2                                1,                 Ondřej Smištík *, Michal Rybníkář , Milan Malaník , Karel Šmejkal , Stefano Dall’Acqua ,                                                             2                                               Ondřej Jurček , Jakub Treml 1                 1  Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, 61200 Brno,                                                      Czech Republic                   2  Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, 61200 Brno,                                                      Czech Republic                 3  Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131                                                        Padua, Italy                                          *Corresponding author: 474476@muni.cz                 Schisandra chinensis (Schisandraceae) is a medicinal plant used in traditional Chinese medicine                                                                                                         1                to treat many diseases. It grows wild in China, Korea, Japan, and  Eastern part of Russia .                Dibenzocyclooctadiene lignans are the main substances with biological and pharmacological                activity. These lignans stimulate liver regeneration, inhibit hepatocarcinogenesis, and suppress                                  2                lipid peroxidation . Dibenzocyclooctadiene  lignans showed  anti-HIV, antioxidant,  and anti-                                       3                inflammatory activities .                Examination  of  compounds  from  S.  chinensis  fruits  has  led  to  the  isolation  and  structural                determination of eight dibenzocyclooctadiene lignans and two aryltetralones. Furthermore, we                isolated  new  sesquiterpene  7,7-dimethyl-11-methylidenespiro[5.5]undec-2-ene-3-carboxylic                acid. These compounds were isolated by extensive chromatographic separation on normal and                                                                            13                                                                    1                reversed phase and identified by comparison of their  H and  C NMR spectra, MS, CD, and                IR spectra with the corresponding literature.                Selected dibenzocyclooctadiene lignans were tested to examine their cytotoxicity (using the                WST-1 assay), anti-inflammatory activity (in LPS-stimulated monocytic leukemia THP-1 NF-                κB cells monitoring their anti-NF-κB activity), and antioxidant activity (using CAA assay). The                WST-1 experiment led to establishing a non-toxic concetration of 10 μM for furhter biological                assays. Only some dibenzocyclooctadiene lignans have shown antioxidant activity. The highest                antioxidant activity was demonstrated by (-)-gomisin J (p < 0.001). This activity corresponded                to approximately 50% of  the  activity of quercetin  (p < 0.001), which served as a positive                control. The anti-inflammatory activity was detected for (−)-gomisin N (p < 0.0001) and (+)-γ-                schisandrin (p < 0.0001) at a concentration of 10 µM. The activity of these compounds was                                                                                                       1                higher than that of the positive control prednisolone (p < 0.0001) at a concentration 2 µM  .                 References:                1.     Rybnikář, M.; Malaník, M.; Šmejkal, K.; Švajdlenka, E.; Shpet, P.; Babica, P.; Dall’Acqua, S.; Smištík,                O.; Jurček, O.; Treml, J. Dibenzocyclooctadiene Lignans from Schisandra Chinensis with Anti-Inflammatory                Effects. Int. J. Mol. Sci. 2024, 25 (6), 3465. https://doi.org/10.3390/ijms25063465.                2.     Lin,  R.-D.;  Mao,  Y.-W.;  Leu,  S.-J.;  Huang,  C.-Y.;  Lee,  M.-H.  The  Immuno-Regulatory  Effects  of                Schisandra Chinensis and Its Constituents on Human Monocytic Leukemia Cells. Molecules 2011, 16 (6), 4836–                4849. https://doi.org/10.3390/molecules16064836.                3.     Choi, Y.-W.; Takamatsu, S.; Khan, S. I.; Srinivas, P. V.; Ferreira, D.; Zhao, J.; Khan, I. A. Schisandrene,                a Dibenzocyclooctadiene Lignan from Schisandra Chinensis:  Structure−Antioxidant Activity Relationships of                Dibenzocyclooctadiene Lignans. J. Nat. Prod. 2006, 69 (3), 356–359. https://doi.org/10.1021/np0503707.                                                                                                             134","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO60                    Phytochemical profile of wild and cultivated Nigella damascena species                                                collected in Sicily (Italy)                                                                                             1                                                                       1                     Angela Sorice *, Erika Truppo *, Severina Pacifico , Antonio Fiorentino , Maurizio                                                   1,                                  1,                                         2,3                                   Bruno , Natale Badalamenti , Monica Scognamiglio    1                                                               2,3                   1  Department of Environmental Biological and Pharmaceutical Sciences and Technologies,                       University of Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100, Caserta, Italy                    2  Department of Biological, Chemical and Pharmaceutical Sciences and Technologies,                          University of Palermo, Viale delle Scienze, Palermo, 90128, Ed. 17, Italy                             3  NBFC, National Biodiversity Future Center, Palermo, 90133, Italy                    *Corresponding authors: angela.sorice@unicampania.it; erika.truppo@unicampania.it                 Nigella damascena L. is an annual herbaceous plant with a Euro-Mediterranean distribution                range, widely used  in the food, pharmaceutical and cosmetic industries, since these plants'                                                                                  1                unique  phytochemical  composition  directly  relates  to  their  virtues . The  current  work  was                addressed to a comparison between the aerial parts of the same plants of N. damascena, both                wild (WND) and cultivated, with white flowers (CWND) and blue flowers (CBND).                Plant material for each sample type (WND, CWND, and CBND) was subjected to a preliminary                                                           2                analysis  using  a  metabolomics  approach .  A  phytochemical  investigation  of  the  three                methanolic extracts was implemented by employing a combination of NMR spectroscopy and                LC-MS  analysis  to  identify  the  metabolites  present  in  the  extracts.  Furthermore,  a  partial                purification of the extracts was conducted by dissolving them in water and partitioning in ethyl                acetate to concentrate the compounds of interest before analysis.                The  study  emphasized  the  distinct  phytochemical  composition  of  N.  damascena  extracts,                uncovering the presence of different phytochemical compounds, including triterpene saponins,                glycosylated flavonoids and other metabolites. The plants, when exposed to different growth                stress  conditions,  were  characterized  by  distinctive  chemical  profiles.  Notably,  the  relative                abundance  of  glycosylated  triterpenes  in  the  extracts  was  higher  in  the WND  and  CBND                extracts compared to CWND. Overall, the chemical analysis provided insights into the diverse                array  of  phytochemical  compounds  present  in  the  N.  damascena  extracts,  highlighting  the                plant's rich chemical profile and potential pharmacological significance. Subsequent studies                will aim to evaluate the biological activity of these new potentially bioactive compounds.                 References:                1.     Toma  C.C.,  Olah  N.K.,  Vlase  L.,  Mogoșan  C.,  Mocan  A.  Comparative  Studies  on  Polyphenolic                Composition, Antioxidant and Diuretic Effects of Nigella sativa L. (Black Cumin) and Nigella damascena L.                (Lady-in-a-Mist) Seeds. Molecules. 2015. 20(6):9560-9574.  https://doi.org/10.3390                2.     Scognamiglio  M.,  Fiumano V.,  D’Abrosca  B.,  Esposito A.,  Choi Y.  H., Verpoorte  R.,  Fiorentino A.                Chemical interactions between plants in Mediterranean vegetation: The influence of selected plant extracts on                Aegilops geniculata metabolome. Phytochemistry. 2014. 106, 69-85. https://doi.org/10.1016                                                                                                               135","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO61                        How flavonoid-C-glycosides are made in the Caryophyllaceae? –                              a contribution to biosynthesis-related genes discovery                                                                              1                                                       1                                   1,                                                                                                      1                    Marta Stafiniak *, Monika Bielecka , Bartosz Pencakowski , Izabela Nawrot-Hadzik ,                                                                                                 3                                                         2                   Weronika Kozłowska , Christoph Weise , Katarzyna Nowis , Łukasz Łaczmański , Adam                                       1                                                                            3                                                        Matkowski                                                                   1                   1  Department of Pharmaceutical Biology and Biotechnology, Wroclaw Medical University,                                                     Wroclaw, Poland                     2  Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany                      3  Laboratory of Genomics \& Bioinformatics, Hirszfeld Institute of Immunology and                                        Experimental Therapy PAS, Wroclaw, Poland                                     *Corresponding author: marta.stafiniak@umw.edu.pl                 Plants from the Caryophyllaceae family are widely known for their saponin content. However,                previous studies have shown a significant quantity and variety of flavonoid C-glycosides, which                have several medicinal properties. An HPLC analysis of Agrostemma githago L. confirmed the                presence of orientin, isoorientin, vitexin and isovitexin.                C-glycosides are less known than the more common O-glycosides and we know even less about                their biosynthesis in the Caryophyllaceae. This study aimed at expanding knowledge about the                crucial steps of flavonoid C-glycosides biosynthesis and its regulation at the molecular level                and was focused on identifying previously unknown C-glycosyltransferases. The plant material                from A. githago was obtained from field cultivation and in vitro cultures. Nucleotide sequences                of homolog genes involved in the plant flavonoid  biosynthetic pathway were  identified  by                RNAseq – including general phenylpropanoid pathway, through flavones pathway (CHS, CHI,                F3’H,  FSI/FSII),  to  hypothetical  C-glycosylflavones  biosynthesis  with  F2H/CYP93G  and,                currently unknown, CGT. Selected sequences were employed for PCR primer design and gene                expression analysis. Metabolic profiling of flavonoid C-glycosides was performed concurrently                using LC-qTOF-MS.                The discovery and characterization of the mechanisms of transcriptional regulation of the C-                glycoside biosynthetic pathway contribute to a significant  increase in knowledge about this                group of flavonoids. Their considerable importance in the prevention of many lifestyle diseases                allows us to expect that the previously poorly understood aspect of the properties of medicinal                plants  among  the  Caryophyllaceae  would  finally  find  an  appropriate  place  in  their  multi-                directional use.                Acknowledgements:  The  study  was  supported  by  Wroclaw  Medical  University,                grant No. SUBK.D030.24.016                References:                1.     Jakimiuk, K.; Wink, M.; Tomczyk, M. Flavonoids of the Caryophyllaceae; Springer Netherlands, 2022;                Vol. 21. https://doi.org/10.1007/s11101-021-09755-3.                2.     Winkel-Shirley, B. Flavonoid Biosynthesis. A Colorful Model for Genetics, Biochemistry, Cell Biology,                and Biotechnology. Plant Physiol. 2001, 126 (June), 485–493.                3.     Ferreyra, M. L. F.; Rodriguez, E.; Casas, M. I.; Labadie, G.; Grotewold, E.; Casati, P. Identification of a                Bifunctional  Maize  C-  and  O-Glucosyltransferase.  J.  Biol.  Chem.  2013,  288  (44),  31678–31688.                https://doi.org/10.1074/jbc.M113.510040.                                                                                                             136","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO62                    Oncostatic activities of plant nutraceuticals in rodent models of breast                                                       carcinoma                                                                                                    1                                                            1                                         1,                                                                                1                 Miroslava Stankovianska *, Lenka Lacková , Alena Mazuraková , Andrea Kapinová , Karol                                                         1                                                                         3                                      2                                  Kajo , Marián Adamkov , Karel Šmejkal , Peter Kubatka  1                       1  Jessenius Faculty of Medicine, Comenius University in Bratislava, Martin, Slovakia                                     2  St. Elisabeth Oncology Institute, Bratislava, Slovakia                                3  Faculty of Pharmacy, Masaryk University, Brno, Czech Republic                                     *Corresponding author: stankovianska.m@gmail.com                 Comprehensive oncology research suggests an important role of phytochemicals or whole plant                foods in the modulation of signaling pathways associated with anticancer action. The original                data of our working group demonstrated the oncostatic efficiency of  low-dose mixtures of                phytochemicals present in specific herbs, spices, or fruits in rodent models of breast carcinoma.                The goal of this study was to assess the anticancer activities of Hippophae rhamnoides L. using                rat  and  mouse  breast  carcinoma  models. H.  rhamnoides (as  a fruit  peel  powder)  was                administered in the diet at two concentrations of 0.3 % (w/w) and 3 % (w/w) during the whole                experiment in chemopreventive rat and therapeutic 4T1 mouse models.                In  the  chemopreventive  model,  sea  buckthorn  significantly  decreased  the  HG/LG  ratio  vs                controls. This result was associated with increased expression of caspase-3 and Bax/Bcl-2 ratio,                and increased levels of histone chemical modifications i.e. H4K16ac and H4K20m3. Moreover,                sea  buckthorn  decreased  expressions  of  VEGF,  Ki67,  MDA,  CD44,  and  EpCam  when                compared to control samples. In addition, the 4T1 mice model showed a decrease in tumor                volume by 48 % (and a decrease by 45 % in mitotic activity index) after sea buckthorn treatment                vs control tumors.                The present study demonstrated significant chemopreventive and therapeutic activities of H.                rhamnoides against experimental breast carcinoma in vivo.                   Acknowledgement: This study was supported by the grant VEGA 1/0045/23.                                                                                                                                    137","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO63                      Environmentally friendly solid-liquid extraction for the recovery of                                          polyphenols from almond okara                                                                           1                                                                                          1                                                         1                                    1,                       Mariam Taha *, Jennifer Samaillie , Sevser Sahpaz , Vincent Roumy , Nicolas                                                  2                                     Blanchemain , Krasimir Dimitrov , Céline Rivière 1                                                                     1                   1  Joint Research Unit 1158, BioEcoAgro, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA,                  Univ. Artois, Univ. Littoral Côte d'Opale, ICV-Institut Charles Viollette, 59650 Villeneuve-                                                      d'Ascq, France                 2  U1008 Controlled Drug Delivery Systems and Biomaterials, Institut National de la Santé et                   de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Lille,                                              University of Lille, Lille, France                                      *Corresponding author: mariam.taha@univ-lille.fr                 The plant-based milk industry represents the largest plant-based food sector today, with an                estimated annual global growth rate of 15.5% from 2022 to 2029. The reasons for this increase                are  dairy  milk  allergy,  lactose  intolerance  and  the  vegan  lifestyle.  However,  like  the                manufacturing processes of other plant-based drinks, almond beverage production generates                considerable quantities of a solid byproduct known as “okara”. Despite its potential valuable                content of bioactive components, such as polyphenols, lipids, dietary fiber and proteins, the                studies on almond okara are scarce.                Our objective is therefore to valorize almond okara, with the aim of promoting a sustainable                approach  with  zero  agro-industrial  waste  and  producing  functional  ingredients  for  the                nutraceutical and cosmetic industries (functional foods, food supplements …).                Almond milk was produced by soaking and blanching almonds for a defined time, then wet                grinding them with ultrapure water at a ratio of 1/9 (w/v). The mixture was then filtered and the                freshly obtained okara was used for characterization and evaluation.                Aqueous and ethanolic solid-liquid extractions were carried out on almond and almond okara.                The  effect  of  several  parameters  was  studied  (solid-to-solvent  ratio,  solvent  concentration,                temperature, extraction time). Our major objective is to study the influence of these parameters                on the recovery of polyphenols as well as on the antioxidant activity. We analyzed the effect of                these parameters on total polyphenol content (TPC), tannin content (TC) and total flavonoid                content (TFC), as well as on the quality of the extracts by determining the antioxidant activity.                Results obtained showed that the polyphenol contents of the almond okara are relatively close                to the values of the almond. In addition, our results showed a significant antioxidant activity of                this by-product.  These results confirm our theory that almond okara is a very promising by-                product that could be implemented at several industrial levels.                 Acknowledgments:  Authors  gratefully  thank  the  Hauts-de-France  Region  for  funding  the                research project of Mariam Taha (STaRS \"Accueil de jeunes chercheurs d’avenir\" program –                projet CPLA).                                                                                                              138","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO64                 Identification of plant phenolics as novel PPARγ agonists and hypoglycemic                                                           agents                                                                         2                                                                                          2                                     1,                         Jakub Treml *, Jiří Václavík , Lenka Molčanová , Marie Čulenová , Scarlet                                                     2                                                         4                                    3                                                                                              4,5                                                                            3                    Hummelbrunner , Cathrina Neuhauser , Verena M. Dirsch , Julian Weghuber , Karel                                                                 2                                                         Šmejkal                       1  Department of Molecular Pharmacy, Masaryk University, Brno, Czech Republic                          2  Department of Natural Drugs, Masaryk University, Brno, Czech Republic                       3  Department of Pharmaceutical Sciences, University of Vienna, Vienna, Austria                 4  Center of Excellence Food Technology and Nutrition, University of Applied Sciences Upper                                                   Austria, Wels, Austria                    5  FFoQSI GmbH-Austrian Competence Centre for Feed and Food Quality, Safety and                                                 Innovation, Tulln, Austria                                       *Corresponding author: tremlj@pharm.muni.cz                 There is a great need in finding new hypoglycemic agents as the number of diabetes mellitus                                         1                patients is rising sharply . The objective of this study was to explore PPARγ agonism and                hypoglycemic  activity  among  plant-derived  phenolic  compounds.  We  started  with  a                preselection  using  in  silico  molecular  docking,  and  later,  the  selected  compounds  were                                                                                                      2                investigated using in vitro cell culture-based assays: PPARγ luciferase reporter gene assay  and                PPARγ protein  expression (by western blot analysis). Moreover, the ability of the selected                compounds to induce GLUT4 translocation in cell culture and lower blood glucose levels in                chicken embryos was determined.                Among the thirty-six plant phenolic compounds, moracin M showed the highest hypoglycemic                effect in an in ovo experiment followed by mulberrofuran Y and diplacone. Neither moracin M                or mulberrofuran Y showed a conclusive effect on enhancement of GLUT4 translocation or                agonism on PPARγ. On the other hand, diplacone increased GLUT4-GFP signal with greater                agonism towards PPARγ. Thus, we believe that compounds moracin M, mulberrofuran Y, and                diplacone are suitable for further experiments in antidiabetic research and elucidation of their                mechanisms of action.                 References:                1.     Acts  \&  figures,  International  Diabetes  Federation  (n.d.).  https://idf.org/about-diabetes/diabetes-facts-                figures/                (accessed January 20, 2024).                2.     Atanasov,  A.  G.  et  al.  Honokiol:  A  non-adipogenic  PPARγ  agonist  from  nature. BBA-  Gen                Subj. 2013, 1830, 4813-4819. https://doi.org/10.1016/j.bbagen.2013.06.021                3.     Haselgrüber,  R.  et  al.  Insulin  Mimetic  Properties  of  Extracts  Prepared  from  Bellis                perennis. Molecules. 2018, 23, 2605. https://doi.org/10.3390/molecules23102605                                                                                                                 139","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO65                   Bioactive small molecules from Mediterranean plants as ligands for DNA                                                 secondary structures                                                                              1                                                                                                3                  Giovanna Valentino *, Nicola Borbone , Brigida D’Abrosca , Giuseppe Gattuso , Giorgia                                                        2                                     1,                                                                          1                                                                                              1                                    4                                                  5                            Oliviero , Serena Riela , Monica Scognamiglio , Antonio Fiorentino                   1  Department of Environmental Biological and Pharmaceutical Sciences and Technologies,                      University of Campania “Luigi Vanvitelli”, via Vivaldi 43 – 81100, Caserta, Italy                 2  Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 –                                                       Naples, Italy                     3  Department of Chemical, Biological, Pharmaceutical and Environmental Sciences,                        University of Messina Viale F. Stagno d'Alcontres 31, 98166 – Messina – Italy                   4  Department of Molecular Medicine and Medical Biotechnologies, University of Naples                                             Federico II, 80131 – Naples, Italy                           5  Department of Chemistry, University of Catania, Via Andrea Doria, 6,                                                   95125 – Catania, Italy                                 *Corresponding author: giovanna.valentino@unicampania.it                  Mediterranean plants have long been used by traditional medicine to treat various disorders.                They contain a wide range of bioactive molecules with distinct chemical structures showing                pharmacological activities . Experimental evidence demonstrated that bioactive molecules play                                         1                a  crucial  role  in  anticancer  therapy  by  targeting  and  interfering  with  biological  processes                responsible  for  tumor  growth  and  progression.  Several  studies  demonstrated  that  cancer                development is strictly associated with structural changes of the genomic DNA that adopts                alternative  secondary  structure.  In  particular,  the  main  gene  expression  regulator  G                quadruplexes (G4s) DNA play an important role in the cancer progression and their stabilization                by  specific  binders  as  natural  ligands  may  open  promising  avenues  for  potential  therapies                against cancer. Phytochemicals may act as ligands for DNA secondary structures in target cells,                                                                                              2,3                interfering with their regulatory activity and acting as potential anti-tumor drugs  . In the aim                to  identify  bioactive  molecules  able  to  interact  specifically  with  genomic  G4s,  different                Mediterranean  plant  extracts  were  screened  by  using  modern  NMR-based  metabolomics                methodologies  allowing  faster  and  improved  discovery  and  characterization  of  potential                biomolecules.  In  particular,  our  methodological  approach,  based  on  the  combination  of                advanced in-mixture NMR experiments with an ad hoc phytochemical strategy allowed us to                isolate  different  secondary  metabolites  such  as  carexanes,  lignans,  alkaloids,  flavonoids.                Interaction studies between the isolated pure metabolites and the G-quadruplex-folded DNA                regulatory regions of the human K-RAS oncogene are currently in progress in our labs. Targeted                delivery  studies  on  the  most  promising  ligands  will  also  be  performed  using  halloysite                nanotubes as drug carriers.                 References:                1.     Twaij, B. M.; Hasan, M. N. Bioactive Secondary Metabolites from Plant Sources: Types, Synthesis, and                Their Therapeutic Uses. Int. J. Plant Biol. 2022, 13 (1), 4-14.                2.     Kosiol, N.; et al. G-quadruplexes: a promising target for cancer therapy. Mol Cancer 2021, 20 (1), 40.                3.     Falanga, A. P.; et al.. Exploring the Relationship between G-Quadruplex Nucleic Acids and Plants: From                Plant G-Quadruplex Function to Phytochemical G4 Ligands with Pharmaceutic Potential. Pharmaceutics 2022,                14 (11). DOI: 10.3390/pharmaceutics14112377.                                                                                                              140","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO66                       Five new tamarixetin glycosides from Astragalus thracicus Griseb.,                  including some substituted with the rare 3-hydroxy-3-methylglutaric acid                                  and their collagenase inhibitory effects in vitro                                                                                       2                                                       2                                                                        2                       Hristo Vasilev 1,2, *, Karel Šmejkal , Sabina Jusková , Jiri Vaclavik , Jakub Treml 3                  1 Department of Pharmacognosy, Faculty of Pharmacy, Medical University, 2 Dunav str.,1000                                                      Sofia, Bulgaria                   2 Department of Natural Drugs, Faculty of Pharmacy, Masaryk University, Palackého tř.                                            1946/1, Brno, 61200, Czech Republic                 3 Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Palackého                                          tř. 1946/1, Brno, 61200, Czech Republic                                     *Corresponding author: christo.vasillev@gmail.com                 Along  with  the  known  kaempferol-3-O-α-L-rhamnopyranosyl-(1→2)-[6-O-(3-hydroxy-3-                methylglutaryl)]-β-D-galactopyranoside (1), five new flavonoids, containing the rarely isolated                aglycon  tamarixetin,  were  isolated  from  methanolic  extract  of  the  endemic  Balkan  species                Astragalus thracicus Griseb. Three of the new compounds are substituted with the untypical                for  genus  Astragalus  3-hydroxy-3-methylglutaryl  residue  (HMG).  The  compounds  were                identified       as        tamarixetin-3-O-α-L-rhamnopyranosyl-(1→2)-[6-O-(3-hydroxy-3-                methylglutaryl)]-β-D-galactopyranoside      (2),    tamarixetin-3-O-(2,6-di-O-α-L-rhamno-                pyranosyl)-β-D-galactopyranoside  (3),  tamarixetin  3-O-β-D-apiofuranosyl-(1→2)-β-D-                galacto-pyranoside    (4),    tamarixetin-3-O-β-D-apiofuranosyl-(1→2)-[6-O-(3-hydroxy-3-                methylglutaryl)]-β-D-galactopyranoside  (5),  and  tamarixetin-3-O-β-D-apiofuranosyl-(1→2)-                [α-L-rhamno-pyranosyl-(1→6)]-β-D-galactopyranoside  (6).  Selected  compounds  from  A.                thracicus  were  tested  for  to  evaluate  their  anticollagenase  activity. The  greatest  effect  was                observed for quercetin-3-O-β-D-apiofuranosyl-(1→2)-β-D-galactopyranoside, possibly due to                a presence of ortho-dihydroxy arrangement of flavonoid ring B. The effect on collagenase and                elastase was further evaluated also by in silico study and the test compounds showed some level                of in silico interaction.                                                                                                                                141","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO67                      Vincarubine: Unraveling a cytotoxic mechanism against cancer cells                   Darja Koutova , Abdul Aziz Timbilla , Karel Kralovec , Jan Korabecny , Martina Rezacova ,                                                                     2                                                                                                         1                                                                                     3                                                    1                               1                                                                    4,                                                    4                                                                                      1                                    Lucie Cahlikova , Rudolf Vrabec *, Radim Havelek                    1  Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles                              University, Simkova 870, Hradec Kralove 500 03, Czech Republic                   2  Department of Biological and Biochemical Sciences, Faculty of Chemical Technology,                         University of Pardubice, Studentska 573, Pardubice 532 10, Czech Republic                   3  Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences,                       University of Defence, Trebesska 1575, Hradec Kralove 500 01, Czech Republic                 4  Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles                    University, Akademika Heyrovskeho 1203/8, Hradec Kralove 500 03, Czech Republic                                         *Corresponding author: vrabecr@faf.cuni.cz                 The naturally occurring monoterpene bis-indole alkaloids vincristine and vinblastine are well-                known and established chemotherapeutics used in the conventional treatment of certain types                of cancer. They act as cytotoxic agents mainly by disrupting microtubule dynamics in cells.                Another naturally occurring alkaloid with a similar structure, vincarubine, has been isolated                from aerial parts of Vinca minor in this study. Given its structure similarity, an initial screening                of vincarubine's cytostatic and cytotoxic activity on a panel of nine tumor and one non-tumor                cell lines was performed. Vincarubine significantly inhibited cell growth at 10  M, more so                than the positive standard doxorubicine  at 1  M. The IC50 values for these cell lines were                determined with a average of 3.5 M. These results encouraged a deeper focus on its mechanism                of action. The effects of vincarubine on cell viability and proliferation in A549, MCF-7, A2780,                and MRC-5 cell lines were further studied using xCELLigence method in real time. The impact                on the cell cycle in A549 cell line suggested that vincarubine perturbs proliferation in the G1                phase, contrary to vincristine. The docking study into the active site of tubulin showed weaker                interaction as well, implying the involvement of different mechanisms. The study on activation                of caspase-3 and -7 indicated that vincarubine induces apoptosis, particularly in Jurkat and                SAOS-2 cell lines, at concentrations of 5 and 10 M. Additionally, vincarubine influence on                the  level  and  organisation  of  proteins  such  as  actin,  tubulin,  paxillin,  and  vinculin  was                determined  through  epifluorescence  imaging  of  A549  cells,  further  suggesting  a  distinct                mechanism of action from vincristine and vinblastine. This study highlights the importance of                exploring naturally occurring compounds for cancer therapy, as evidenced by vincarubine's                specific mechanism of action and its potential to offer a new therapeutic approach.                 Acknowledgements: Authors wish to thank the project SVV-260-662, SVV-2023-260656 and                Cooperatio Program, research area DIAG of the Charles University.                                                                                                               142","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO68                  Semi-targeted metabolomics of Selaginella plants based on LC-MS coupled                              with bioactivity assay for identification of biomarkers                                           1                                                                     1                                                                                           2                      Sutthida Wongsuwan , Watthanachai Jumpathong , Wanlaya Thamnarak , Jaruwan                                                                  1,2                                             1                                                                                       1,2,                                 Chatwichien , Somsak Ruchirawat , Nopporn Thasana *                    1 Program in Chemical Sciences, Chulabhorn Graduate Institute, Center of Excellence on                 environmental Health and Toxicology (EHT), Ministry of Education, 906 Kamphaeng Phet 6                                           Road Laksi, Bangkok, 10210, Thailand                 2 Laboratory of Medicinal Chemistry, Chulabhorn Research Institute, 54 Kamphaeng Phet 6                                           Road Laksi, Bangkok, 10210, Thailand                       *Corresponding author: nopporn@cri.or.th; presenting author: sutthida@cgi.ac.th                 The single genus Selaginella P. Beauv. (Selaginellaceae) is an important plant resource with                renowned for its diverse applications in food and medicinal chemistry. Selaginella plants are                recognized for their abundance in bioactive biflavonoids (BFVs). Although the presence of                BFVs  has  been  documented  in  numerous  species,  a  comprehensive  metabolomic  and                bioactivity assessment of various plant species in Thailand remains absent. Biochemometrics,                which integrates chemical profiles with biological data, serves as a robust methodology for                analyzing and identifying bioactive compounds within intricate mixtures such as fractions or                crude extracts. This study aims to establish semi-targeted metabolomic profiling of Selaginella                plants  from  different  regions  in Thailand  through  a  methodology  centered  on  LC-MS  and                bioassay-guided approaches. HPLC analysis of EtOAc extracts from Selaginella plants revealed                several BFVs, notably amentoflavone, which is extensively acknowledged for its pharmacological                properties.  The  disparities  in  metabolite  profiles  among  Selaginella  species  from  diverse                geographical locations were scrutinized using multivariate analysis. Unsupervised analysis of                this  dataset  showcases  the  efficacy  of  the  analytical  workflow  in  discriminating  between                different  Selaginella  species.  Coupled  with  supervised  analysis,  a  roster  of  metabolites                responsible for the anti-inflammatory activity under investigation was delineated. This study                serves as a valuable tool for expeditious dereplication and identification of known or unknown                BFVs.  It  offers  potential  for  amalgamating  data  from  multiple  stages  of  fractionation  and                bioassays into a cohesive analysis framework.                Acknowledgements: This project is supported by Chulabhorn Royal Academy (Fundamental                Fund  by  National  Science  Research  and  Innovation  Fund  (NSRF):  fiscal  year  2024).  The                authors thank to Program in Chemical Sciences and Program in Environmental Toxicology,                Chulabhorn Graduate Institute (CGI) for the instrumentation and cell culture facilities. SW                thank to CGI postdoctoral followship for financial support (Grant No. CGI(2022/03).                 References:                1.     Demehin, A. A.; Thamnarak, W.; Lamtha, T.; Chatwichien, J.; Eurtivong, C.; Choowongkomon, K.;                Chainok, K.; Ruchirawat, S.; Thasana, N. Phytochemistry. 2022, 203, 113374.                2.     Kellogg, J. J.; Todd, D. A.; Egan, J. M.; Raja, H. A.; Oberlies, N. H.; Kvalheim, O. M.; Cech, N. B. J.                Nat. Prod. 2016, 79, 376-386. https://doi.org/10.1021/acs.jnatprod.5b01014                3.     Wang, B.; Lu, Y.; Hu, X.; Feng, J.; Shen, W.; Wang, R.; Wang, H. J. Agric. Food Chem. 2020, 68,                14808-14823. https://doi.org/10.1021/acs.jafc.0c04532                                                                                                              143","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO69                   The analysis of the in vitro flax plant with tocopherol-pathway gene VTE2                                                      overexpression                                                                      2                                       Iwan Zalewski *, Anna Kulma , Anna Prescha   1                                                     1,                                  1  Wroclaw Medical University, Pasteura 1, 50-367 Wrocław                                 2  Wroclaw University, pl. Uniwersytecki 1, 50-137 Wrocław                                     *Corresponding author: iwan.zalewski@umw.edu.pl                 The flax is one of the oldest plants domesticated by human and having unique characteristics.                It provides fibers for the textile industry and the oil used in the food, cosmetic, chemical and                                         1                pharmaceutical  industries .  Flax  seed  oil  contains  a  large  number  of  biologically  active                components. One of these components are tocopherols, which are synthesized exclusively by                plants.  In  plants,  tocopherols  function  to  protect  cell  membranes  from  oxidative  stress.  In                humans, tocopherols from food play important role in the prevention of neurodegenerative,                cardiovascular diseases and cancer. The antioxidant properties of these components come from                the ability to reduce free radicals, before their interaction with the biological membrane . In this                                                                                                   2                work we overexpressed in the flax plants the vte2 gene, which encodes homogentisate phytyl                transferase  from  Arabidopsis  thaliana.  The  enzyme  produces  the  first  substrate  in  the                                                                                                   3                tocopherol pathway and is considered as a key enzyme in the synthesis of tocopherols .                This report shows the analysis of the transgene plants. The introduced gene was confirmed by                PCR; the tocochromanols were determined by ultra-high performance liquid chromatography                (UPLC).  The  terpenoid  pathways  activity  was  checked  by  real-time  PCR  (MEP,  MEV,                carotenoids, tocochromanols) and the content of the main metabolites formed in these pathways                checked  by  UPLC  (tocochromanols,  carotenoids,  chlorophylls).  Also,  the  effect  of  the                modifications performed on the levels of metabolites of the tocopherol synthesis pathway also                reflected  the  antioxidant  potential  of  the  plant  extracts. The  resistance  of  transgene  plants                against  fungal  infections  using  Fusarium  culmorum  and  Fusarium  oxysporum  was  also                examined.                  References:                1.     Morris, M. V.-G. A. D. H. Introduction: History of the Cultivation and Uses of Flaxseed. In Flax; CRC                Press, 2003.                2.     Saldeen, K.; Saldeen, T. Importance of Tocopherols beyond α-Tocopherol: Evidence from Animal and                Human Studies. Nutr. Res. 2005, 25 (10), 877–889. https://doi.org/10.1016/j.nutres.2005.09.019.                3.     Collakova, E.; DellaPenna, D. Homogentisate Phytyltransferase Activity Is Limiting for Tocopherol                Biosynthesis in Arabidopsis. Plant Physiol. 2003, 131 (2), 632–642. https://doi.org/10.1104/pp.015222.                                                                                                                    144","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO70                   Biotic stress as modulator of isoquinoline alkaloid profile in Chelidonium                                      majus in hydroponic and in vitro cultures                                                                                         1                                                                                                        1                  Sylwia Zielińska *, Weronika Kozłowska , Anna Jezierska-Domaradzka , Maria Malicka ,                                                          1                                  1,                                                                   2                                                                                      3                                                     2                                    Malwina Brożyna , Adam Junka , Adam Matkowski                    1  Division of Pharmaceutical Biotechnology, Department of Pharmaceutical Biology and                                Biotechnology, Wroclaw Medical University, Wroclaw, Poland                 2  Platform for Unique Models Application, Department of Pharmaceutical Microbiology and                                Parasitology, Wroclaw Medical University, Wroclaw, Poland                  3  Botanical Garden of Medicinal Plants, Departmentsevda of Pharmaceutical Biology and                                Biotechnology, Wroclaw Medical University, Wroclaw, Poland                                    *Corresponding author: sylwia.zielinska@umw.edu.pl                 Chelidonium majus L. metabolic response to biotic stress was investigated using elicitation wih                Pseudomonas  luteola  and  P.  aeruginosa  as  well  as  the  stress  signaling  mediator  methyl                jasmonate (MeJa). In vitro shoots cultured on MH3 solid media under white light (WL) or                photosynthetic active radiation (PAR), as well as callus cultured on MH3 medium with kinetin,                NAA, and 2,4D were treated with lyophilized P. luteola cells [0.02, 0.2% suspension] and MeJa                [100 µM]. In the hydroponic system, P. aeruginosa [1, 0.2, 0.02%] and MeJa [100 µM] were                used.  Following  the  application  of  biotic  elicitors,  the  isoquinoline  alkaloid  profile  was                analyzed using chromatographic techniques (HPLC, LC-MS/MS). PAR illumination resulted                in higher content of allocryptopine and coptisine in in vitro shoots treated with biotic elicitors                as well as MeJa, compared to untreated plant material and WL. In the callus cultures, P. luteola                [0.2%]  influenced  the  content  protopine  and  chelerythrine,  compared  to  untreated  plants.                Conversely,  a  lower  bioelicitor  concentration  [0.02%]  was  more  effective  for  sanguinarine                increase. However, the ultimate outcome in both in vitro culture types, shoots and callus, may                also be affected by various culture parameters, including light exposure and the developmental                phase of the plant. In the hydroponic cultures, the application of P. aeruginosa [0.2%] was most                effective in boosting the content of protoberberine and benzophenanthridine derivatives such                as  coptisine,  berberine,  chelidonine,  sanguinarine  and  chelerythrine.  Nevertheless,  in  all                experiments, the influence of MeJa on isoquinoline alkaloid content was most noticeable. This                investigation  highlights  the  need  for  further  research  into  the  specific  effects  of  microbial                strains, including optimal concentrations and exposure durations, on both in vitro and in vivo                grown plants. Such studies are crucial for elucidating the mechanisms of action and optimizing                the production of specialized metabolites.                 Acknowledgements: The  study  was  supported  by  the  National  Research  Center  of  Poland                (NCN), Grant No.: SONATA 15, 2019/35/D/NZ7/00266.                                                                                                               145","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO71                     Anti-Inflammatory Effect and Cytotoxic Properties of Glaucium flavum                                                         Extracts                                                           1                                                                               2                                                                                               1                    Marcel Białas 1#* , Weronika Kozłowska , Monika Czerwińska , Maria Malicka , Adam                                                         3                                                                            1                                               Matkowski , Sylwia Zielińska                   1  Division of Pharmaceutical Biotechnology, Department of Pharmaceutical Biology and                    Biotechnology, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland;                                                  1# Student Scientific Club                     2  Department of Biochemistry and Pharmacogenomics, Faculty of Pharmacy, Medical                                University of Warsaw, Banacha 1, 02-097 Warszawa, Poland                   3  Division of Pharmaceutical Biology and Botany, Department of Pharmaceutical Biology                  and Biotechnology, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland                                   *Corresponding author: marcel.bialas@student.umw.edu.pl                   Glaucium flavum Crantz, commonly known as yellow horn poppy (Papaveraceae) is a coastal                herb indigenous to i.a. Europe, Northern Africa (Macaronesia). Due to interesting metabolic                profile,  G.  flavum  extracts  derived  from  organ  cultures  in  vitro  were  investigated  for  their                potential anti-inflammatory and cytotoxic effect. G. flavum shoots were cultured on MH3 solid                media supplemented with kinetin (KIN) at concentrations of 2.5, 4.5, and 7µM, along with                indole-3-acetic acid at a concentration of 0.5 µM. Methanolic extracts were tested for their                cytotoxicity and the effect on IL-1, IL-8, and TNF-α secretion in human neutrophil model. For                comparative  analysis  methanolic  and  ethanolic  extracts  from  roots,  stems  with  leaves  and                flowers of plants cultivated in two botanical gardens (Kraków and Lublin) were used.                 The extracts tested demonstrated no cytotoxic effects on neutrophil cells. Their capacity to                inhibit secretion varied with concentration and was distinct across different extracted organs                and specific cytokines. For TNF-α, the stem extract from Lublin exhibited the most potent                inhibitory effect at a concentration of 1 µg/ml. Regarding IL-1 and IL-8, the extracts showing                the greatest efficacy were from in vitro shoots grown on MH3 medium containing 7 µM of KIN                and from shoots on non-supplemented 1/2 MH3 medium, each at 1 µg/mL, respectively. Further                investigation is essential to identify the phytochemical profiles of these extracts and to indicate                the group of compounds directly contributing to the observed bioactivities.                 Acknowledgements: The study was funded by the National Science Centre of Poland: SONATA                15, 2019/35/D/NZ7/00266 “Biotic and abiotic stress elicitors as modulators of isoquinoline                alkaloid  profile  towards  specific  antimicrobial  properties  of  medicinal  plants  from  the                Papaveraceae” to Sylwia Zielińska.                                                                                                               146","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                PO72                     Bug vs Bug: Use of Galleria mellonella larvae to test activity of Essential                           Oils and their components against drug-resistant microbes                                          1,*                                                                                                    1                                                            1                                                                                 1                      Bartłomiej Dudek , Malwina Brożyna , Aleksandra Woytoń , Natalia Tymińska ,                                                       Adam Junka  1                  1 Platform for Unique Models Application, Department of Pharmaceutical Microbiology and                             Parasitology, Wroclaw Medical University, 50-556 Wroclaw, Poland                                     *Corresponding author: bartlomiej.dudek@umw.edu.pl                   Galleria  mellonella,  an  invertebrate  model  organism,  presents  a  valuable  alternative  to                traditional mammalian infection models, offering several advantages for  scientific research.                This model bypasses the necessity for bioethics committee approval, due to its invertebrate                status, and provides a cost-effective and efficient solution for cultivation. G. mellonella larvae                are notable for their production of antimicrobial compounds and the circulation of hemolymph,                supported by diverse cell lines, which are instrumental for research purposes.The increasing                emphasis on reducing the use of higher animals in laboratory settings and the shift towards                alternative model organisms underscore the significance of G. mellonella in studying bacterial                pathogenicity. A pivotal attribute of G. mellonella, particularly in researching human pathogens,                is its ability to be cultured at 37˚C, paralleling some fundamental aspects of the human immune                response both structurally and physiologically. This makes G. mellonella an exemplary model                for cytotoxicity evaluations and for determining the efficacy of antimicrobial agents, including                antibiotics  and  disinfectants.  Our  study  introduces  an  innovative  approach  by  utilizing  the                external integuments of G. mellonella larvae as a platform for inducing localized infections                associated with biofilms, aiming to assess the effectiveness of antimicrobial treatments. We                explored the use of thyme essential oil (T-EO) as a potential antibiofilm agent against bacterial                biofilm-related infections. Remarkably, the application of T-EO proved to be non-toxic to the                larvae and significantly enhanced their survival rate when infected with Staphylococcus aureus,                from 48% to 85%. These findings highlight T-EO's potential as an effective antimicrobial agent                for biofilm-associated infections and affirm the utility of the G. mellonella model in mimicking                the conditions of multicellular organisms, offering promising avenues for future antimicrobial                research.                   Acnowledgments: Authors thank to Medical University of Wroclaw for providing resources                within grant numer SUBK.D230.24.039                                                                                                                147","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                                  List of participants                                      Ademola Adekoya                                     Masaryk University, Brno, Czech Republic                 OL34                Fabian Alperth                                     Institute of Pharmaceutical Sciences, Department of Pharmacognosy,                                     University of Graz, Graz, Austria                 PL7                 Giovanni Appendino                                     Dipartimento di Scienze del Farmaco, University of Novara, Novara,                                     Italy                 PL21                Randolph Arroo                                     De Montfort University, Leicester School of Pharmacy, Leicester, UK                 PL6                 Petr Babula                                     Masaryk University, Brno, Czech Republic                 PO1, PO10           Miriam Bačkorová                                     University  of  Veterinary  Medicine  and  Pharmacy,  Košice,  Slovak                                     Republic                                     Sevda Deniz Bakir                                     Faculty of Pharmacy, Hacettepe University, TR-06100, Ankara, Turkey                 PL16, PO15          Szabolcs Béni                                     Institute of Chemistry, Eötvös Loránd University, Budapest, Hungary                 PO2                 Kateřina Berková                                     Faculty of Tropical AgriSciences, Czech University of Life Sciences                                     Prague, Praha, Czech Republic                 P71                 Marcel Białas                                     Wroclaw Medical University, Wroclaw, Poland                 PO3, PO33, PO34  Monika Bielecka                 PO48, PO61          Wroclaw Medical University, Wroclaw, Poland                 PO4, PO11,          Silvia Bittner Fialová                 PO32, PO35          Comenius University Bratislava, Faculty of Pharmacy, Bratislava,                                     Slovakia                 OL19                Shamee Bhattacharjee                                     West Bengal State University, Kolkata, India                 OL28                Augustin Bildstein                                     University Lille, Lille, France                                     Hana Brožová                                     Masaryk University, Brno, Czech Republic                 PO5, OL11,          Malwina Brożyna                 PO70                Wrocław Medical University, Wrocław, Poland                 PL15, OL30,         Franz Bucar                 OL34                Graz University, Graz, Austria                 PO6                 Beata Budíková                                     Institute of Experimental Botany of the Czech Academy of Sciences \&                                     Palacký University, Olomouc, Czech Republic                 PL23, OL21,         Lucie Cahlíková                 OL24, PO49          Charles University, Hradec Králové, Czech Republic                                                                                                                   148","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 OL37                Micol Caichiolo                                     University of Padova, Padua, Italy                 PO7                 Kateřina Cermanová                                     Institute of Experimental Botany of the Czech Academy of Sciences \&                                     Palacký University, Olomouc, Czech Republic                                     Tomáš Chára                 PO22, PO27          Subhasis Chattopadhyay                                     Masaryk University, Brno, Czech Republic                 OL23, PL11          Chiara Collevecchio                                     University “Gabriele d’Annunzio” of Chieti-Pescara, Chieti Scalo                                     (CH), Italy                 PO8, OL31,          Luísa Custódio                 PO53, PO9           University of Algarve, Faculty of Sciences and Technology, Faro,                                     Portugal                 PO10, PO1           Eva Čonková                                     University of Veterinary Medicine and Pharmacy, Košice, Slovak                                     Republic                 OL1                 Kateřina Dadáková                                     Masaryk University, Brno, Czech Republic                 PL22, PO59          Stefano Dall'Acqua                                     University of Padua, Padua, Italy                 PO11, PL13,         Kamila Dokupilová                 PO4, PO32, PO35  Faculty of Pharmacy, Comenius University, Bratislava, Slovakia                 PO72                Bartłomiej Dudek                                     Wroclaw Medical University, Wroclaw, Poland                 PO12                Margita Dvorská                                     Masaryk University, Brno, Czech Republic                 PL11, OL23          Francesco Epifano                                     University “Gabriele d’Annunzio” of Chieti-Pescara, Chieti Scalo                                     (CH), Italy                 PO13                Magdalena Fast                                     Wroclaw Medical University, Wroclaw, Poland                 PL2                 Maria José Umbelino Ferreira                                     Universidade de Lisboa, Lizbon, Portugal                 OL22, PO60,         Antonio Fiorentino                 PO65                Università degli Studi della Campania Luigi Vanvitelli, Caserta,                                     Campania, Italy                 PO14                Barbora Fiserova                                     Czech University of Life Sciences Prague, Praha, Czech Republic                 PO15                Mária Gáborová                                     Masaryk University, Brno, Czech Republic                 OL4                 Ernesto Gargiulo                                     University of Naples Federico II, Napoli, Italy                 OL3, PO50           Magdalena Grajzer                                     Wroclaw Medical University, Wroclaw, Poland                 OL29, OL27          Kajetan Grzelka                                     Wroclaw Medical University, Wroclaw, Poland                 OL6, PO52           Francesca Guzzo                                     University of Campania \"Luigi Vanvitelli\", Caserta, Italy                                                                                                                149","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 OL8                 Jan Günther                                     University of Copenhagen, Frederiksberg C, Denmark                 PO16                Jiří Hanyk                                     Faculty of Medicine and Dentistry, Palacký University, Olomouc,                                     Czech Republic                 PO17, PO39          Patrícia Harčárová                                     Charles University, Faculty of Pharmacy in Hradec Králové, Czech                                     Republic                                     Simona Hašanová                                     Charles University, Faculty of Pharmacy in Hradec Králové, Czech                                     Republic                 OL16, PL23,         Radim Havelek                 PO67                Faculty of Pharmacy, Charles University, Hradec Kralove, Czech                                     Republic                 PO18                Martin Helcman                                     Masaryk University, Brno, Czech Republic                 PO19                Pavel Hladík                                     Institute of Experimental Botany, The Czech Academy of Sciences \&                                     Faculty of Science, Palacký University, Olomouc, Czech Republic                                     Daniela Hlavatá                                     Masaryk University, Brno, Czech Republic                 OL15                Iasmina Honceriu                                     “Alexandru Ioan Cuza” University of Iasi, Iasi, Romania                                     Ježková Martina                                     Masaryk University, Brno, Czech Republic                 PO20                Jan Hošek                                     Masaryk University, Brno, Czech Republic                 PO21, PO14          Marketa Houdkova                                     Czech University of Life Sciences Prague, Prague, Czech Republic                 OL12                Selen İlgün                                     Faculty of Pharmacy, Erciyes University, Kayseri, Türkiye                 OL14, PO38          Valeria Iobbi                                     University of Genova, Genova, Italy                 PO23, PO24          Smiljana Janković                                     University of Belgrade, Faculty of Biology, Belgrade, Serbia                 PO25                Dagmar Jankovská                                     Masaryk University, Brno, Czech Republic                 PO55                Eliška Jeníčková                                     Czech University of Life Sciences Prague, Prague, Czech Republic                 PO27, OL8           Shen Jincheng                                     University of Copenhagen, Frederiksberg C, Denmark                 OL11, PO6           Adam Junka                                     Wrocław Medical University, Wrocław, Poland                 PO27, PO59,         Ondřej Jurček                 PO54, PO22,         Masaryk University, Brno, Czech Republic                 PO15                 PO28                Iva Jurčević Šangut                                     University North, Department of Food Technology, Koprivnica,                                     Croatia                                                                                                                150","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 PO30, OL10          Sylvia Kalli                                     Wageningen University \& Research, Wageningen, the Netherlands                 OL34, PO30          Jonas Kappen                                     Leibniz Institute of Plant Biochemistry (IPB), Halle (Saale), Germany                 OL17                Melek Karaaslan                                     Ankara University, Faculty of Pharmacy, Ankara                 OL24, OL25,         Hasan Kırmızıbekmez                 OL35                Faculty of Pharmacy, Yeditepe University, İstanbul, Türkiye                 OL18                Adrian H. Kopf                                     Wageningen University \& Research, Wageningen, the Netherlands                 OL22                Chidoh Kootlole                                     University of Campania “Luigi Vanvitelli”, Caserta, Italy                 PO32, PL13,         Ema Kostovčíková                 PO4, PO11,          Faculty of Pharmacy, Comenius University, Bratislava, Slovakia                 PO32, PO35                 OL16, PO67          Darja Koutova                                     Faculty of Pharmacy, Charles University, Hradec Králové, Czech                                     Republic                 PO33, PO34,         Weronika Kozłowska                 PO3, PO48,          Wrocław Medical University, Wrocław, Poland                 PO61, PO70                 OL21, OL24          Jana Křoustková                                     Faculty of Pharmacy, Charles University, Hradec Králové, Czech                                     Republic                                     Michaela Kuchynka                                     Faculty of Pharmacy, Masaryk University, Brno, Czech Republic                 OL32                Senthil Kumar Kanthasamy Jayabal                                     National Chung Hsing University, Taichung, Taiwan                 PO35, PL12,         Elena Kurin                 PO4, PO12, PO32  Faculty of Pharmacy, Comenius University, Bratislava, Slovakia                 PO36                Slavomír Kurhajec                                     University of Veterinary Medicine and Pharmacy in Košice, Košice,                                     Slovakia                 OL36                Vahap Murat Kutluay                                     Faculty of Pharmacy, Hacettepe University, Ankara, Türkiye                 PO37                Jovana Lisičić                                     Masaryk University, Brno, Czech Republic                 PO38, OL14          Marta Lo Vetere                                     University of Genova, Genova, Italy                 PO39, PO17          Zuzana Lomozová                                     Charles University, Faculty of Pharmacy in Hradec Králové, Czech                                     Republic                 PO40, PO41          Agnieszka Łubek-Nguyen                                     Medical University of Lublin, Lublin, Poland                 PL17                Simon Vlad Luca                                     Technical University of Munich, Freising, Germany                 PL10                Meghan Lucas                                     Chemistry \& Biodiversity, Wiley-VCH, Weinheim, Germany                 PL1, PL20, OL6      Francisco A. Macías                                     University of Cadiz, Cadiz, Spain                                                                                                               151","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 PO42, PO12,         Milan Malaník                 PO25, PO37,         Masaryk University, Brno, Czech Republic                 PO54, PO59                 PO43                Juraj Markuš                                     Masaryk University, Brno, Czech Republic                 PL24, OL27,         Adam Matkowski                 OL29, PO3,          Wroclaw Medical University, Wroclaw, Poland                 PO13, PO33,                 PO34, PO45,                 PO48, PO58,                 PO61, PO70                 PO44, PO45,         Lenka Molčanová                 PO64                Masaryk University, Brno, Czech Republic                 PL13, PO4,          Pavel Mučaji                 PO11, PO32,         Faculty of Pharmacy, Comenius University, Bratislava, Slovakia                 PO35                 PL8, PL5            Lutfun Nahar                                     Palacký University and Institute of Experimental Botany, The Czech                                     Academy of Sciences, Olomouc, Czech Republic                 PO45                Izabela Nawrot-Hadzik                                     Agrostemma githago as a rich source of flavonoid-C-glycosides and                                     triterpene saponins                 PO46                Daniela Nykodýmová                                     Masaryk University, Brno, Czech Republic                 PO47                Won Keun Oh                                     College of Pharmacy, Seoul National University, Seoul, Republic of                                     Korea                                     Klára Odehnalová                                     Masaryk University, Brno, Czech Republic                 OL35                Ceren Öztürk                                     Faculty of Pharmacy, Yeditepe University, İstanbul, Türkiye                 PO48, PO3,          Bartosz Pencakowski                 PO33, PO61          Wroclaw Medical University, Wroclaw, Poland                 PO49, OL21          Filip Pidaný                                     Faculty of Pharmacy, Charles University, Hradec Králové, Czech                                     Republic                                     Hana Pížová                                     Faculty of Pharmacy, Masaryk University, Brno, Czech Republic                 OL38                Derya Çiçek Polat                                     Ankara University, Faculty of Pharmacy, Ankara, Türkiye                 PO50, PO70,         Anna Prescha                 OL3                 Wroclaw Medical University, Wroclaw, Poland                 PO51                Péter Püski                                     Institute of Pharmacognosy, University of Szeged, Szeged, Hungary                 PO52                Carlo Raucci                                     University of Campania Luigi Vanvitelli, Caserta, Italy                 OL26                Livia Reguengo                                     University of Campinas (UNICAMP), Campinas, SP, Brazil                 PL12, OL28          Céline Rivière                                     Université de Lille, Lille, France                 PL5                 Kenneth Ritchie                                     John Moores University Liverpool, Liverpool, UK                                                                                                            152","PSE Trends in Natural Products 2024 Young Scientists' Meeting                      th                  st                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                 OL20                Janniek Ritsema                                     Wageningen University \& Research, Wageningen, The Netherlands                 OL31, PO53,         Maria João Rodrigues                 PO9                 University of Algarve, Faculty of Sciences and Technology, Faro,                                     Portugal                 PO55                Chandrama Roy Chowdhury                                     Czech University of Life Sciences, Prague, Czech Republic                 PO54                Michal Rybnikář                                     Masaryk University, Brno, Czech Republic                 PL4, PL5            Satya Sarker                                     John Moores University Liverpool, Liverpool, UK                 OL25                Nisa Beril Sen                                     Faculty of Pharmacy, Yeditepe University, Istanbul, Türkiye                 PO56                Katja Schoss                                     University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia                 OL22, PO60,         Monica Scognamiglio                 PO65                University of Campania “Luigi Vanvitelli”, Caserta, Italy                 PO54                Polina Shpet                                     Masaryk University, Brno, Czech Republic                                     Patrik Sivulič                                     Masaryk University, Brno, Czech Republic                 PO56                Gabriela Škovranová                                     Masaryk University, Brno, Czech Republic                 OL27, OL29          Sylwester Ślusarczyk                                     Wroclaw Medical University, Wrocław, Poland                 PO58, PO45          Aleksander Smakosz                                     Wroclaw Medical University, Wrocław, Poland                 PO18, PO36,         Karel Šmejkal                 PO43, PO54,         Masaryk University, Brno, Czech Republic                 PO59, PO62,                 PO64, PO66                 PO59                Ondřej Smištík                                     Masaryk University, Brno, Czech Republic                 PL3, PL21           Didem Şöhretoğlu                 PO29                Hacetteppe University, Ankara, Turkey                 PO60                Angela Sorice                                     University of Campania “Luigi Vanvitelli”, Caserta, Italy                 PO61, PO3,          Marta Stafiniak                 PO33, PO34,         Wroclaw Medical University, Wrocław, Poland                 PO48                 PO62                Miroslava Stankovianska                                     Jessenius Faculty of Medicine, Comenius University in Bratislava,                                     Martin, Slovakia                 OL2                 Jonas Stehlin                                     ZHAW Zurich University of Applied Sciences, Wädenswil, Switzerland                 OL5                 Veronika Stoilkovska Gjorgievska                                     Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje,                                     Republic of North Macedonia                 PL14                Miroslav Strnad                                     Institute of Experimental Botany, The Czech Academy of Sciences \&                                     Faculty of Science, Palacký University, Olomouc, Czech Republic                 PL22                Stefania Sut                                                                                                           153","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                     Department of Pharmaceutical and Pharmacological Sciences                                     University of Padova, Padova, Italy                 PO63                Mariam Taha                                     Univiersity Lille, Lille, France                 OL30                Cristina Terenzi                                     University of Bologna, Rimini, Italy                 OL13                Katerina Tomisova                                     Czech University of Life Sciences Prague, Prague, Czech Republic                                     Ekaterina-MichaelaTomou                                     National and Kapodistrian University of Athens, Athens, Greece                 PO64, PO18,         Jakub Treml                 PO43, PO46,         Masaryk University, Brno, Czech Republic                 PO59,                 PO60                Erika Truppo                                     University of Campania “Luigi Vanvitelli”, Caserta, Italy                 PO25, PO64,         Jiří Václavík                 PO66                Masaryk University, Brno, Czech Republic                 PO65                Giovanna Valentino                                     University of Campania “Luigi Vanvitelli”, Caserta, Italy                 PO66                Hristo Vasilev                                     Masaryk University, Brno, Czech Republic                                     David Vetchý                                     Masaryk University, Brno, Czech Republic                 PO67                Rudolf Vrabec                                     Faculty of Pharmacy, Charles University, Hradec Kralove, Czech                                     Republic                 PL19, OL2           Evelyn Wolfram                                     ZHAW Zurich University of Applied Sciences, Wädenswil, Switzerland                 PO68                Sutthida Wongsuwan                                     Chulabhorn Graduate Institute, Center of Excellence on environmental                                     Health and Toxicology (EHT), Bangkok, Thailand                 OL9                 Hafize Yuca                                     Faculty of Pharmacy, Atatürk University, Erzurum, Turkey                 PO69                Iwan Zalewski                                     Wroclaw Medical University, Wrocław, Poland                 OL7                 Chao Zhang                                     Institute of Experimental Botany of the Czech Academy of Sciences \& Faculty                                     of Science, UPOL, Olomouc, Czech Republic                 PL18                Christian Zidorn                                     Christian-Albrechts- Universität zu Kiel, Kiel, Germany                 PO70, PO33,         Sylwia Zielińska                 PO34                Wroclaw Medical University, Wrocław, Poland                 PL20                Jesús G. Zorrilla                                     University of Cadiz, Puerto Real, Spain                                                                                                                         154","PSE Trends in Natural Products 2024 Young Scientists' Meeting                  st                      th                21  - 24  May 2024, Brno, Czech Republic                BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS                                                         Partners"];